Abstract: The main objective of this research was to investigate
the biosorption capacity for biofilms of sulphate reducing bacteria
(SRB) to remove heavy metals, such as Zn, Pb and Cd from
rainwater using laboratory-scale reactors containing mixed support
media. Evidence showed that biosorption had contributed to removal
of heavy metals including Zn, Pb and Cd in presence of SRB and
SRB were also found in the aqueous samples from reactors.
However, the SRB and specific families (Desulfobacteriaceae and
Desulfovibrionaceae) were found mainly in the biomass samples
taken from all reactors at the end of the experiment. EDX-analysis
of reactor solids at end of experiment showed that heavy metals Zn,
Pb and Cd had also accumulated in these precipitates.
Abstract: The equilibrium, thermodynamics and kinetics of the
biosorption of Cd (II) and Pb(II) by a Spore Forming Bacillus (MGL
75) were investigated at different experimental conditions. The
Langmuir and Freundlich, and Dubinin-Radushkevich (D-R)
equilibrium adsorption models were applied to describe the
biosorption of the metal ions by MGL 75 biomass. The Langmuir
model fitted the equilibrium data better than the other models.
Maximum adsorption capacities q max for lead (II) and cadmium (II)
were found equal to 158.73mg/g and 91.74 mg/g by Langmuir model.
The values of the mean free energy determined with the D-R equation
showed that adsorption process is a physiosorption process. The
thermodynamic parameters Gibbs free energy (ΔG°), enthalpy (ΔH°),
and entropy (ΔS°) changes were also calculated, and the values
indicated that the biosorption process was exothermic and
spontaneous. Experiment data were also used to study biosorption
kinetics using pseudo-first-order and pseudo-second-order kinetic
models. Kinetic parameters, rate constants, equilibrium sorption
capacities and related correlation coefficients were calculated and
discussed. The results showed that the biosorption processes of both
metal ions followed well pseudo-second-order kinetics.