Abstract: Cytogenetic analysis still remains the gold standard method for prenatal diagnosis of trisomy 21 (Down syndrome, DS). Nevertheless, the conventional cytogenetic analysis needs live cultured cells and is too time-consuming for clinical application. In contrast, molecular methods such as FISH, QF-PCR, MLPA and quantitative Real-time PCR are rapid assays with results available in 24h. In the present study, we have successfully used a novel MGB TaqMan probe-based real time PCR assay for rapid diagnosis of trisomy 21 status in Down syndrome samples. We have also compared the results of this molecular method with corresponding results obtained by the cytogenetic analysis. Blood samples obtained from DS patients (n=25) and normal controls (n=20) were tested by quantitative Real-time PCR in parallel to standard G-banding analysis. Genomic DNA was extracted from peripheral blood lymphocytes. A high precision TaqMan probe quantitative Real-time PCR assay was developed to determine the gene dosage of DSCAM (target gene on 21q22.2) relative to PMP22 (reference gene on 17p11.2). The DSCAM/PMP22 ratio was calculated according to the formula; ratio=2 -ΔΔCT. The quantitative Real-time PCR was able to distinguish between trisomy 21 samples and normal controls with the gene ratios of 1.49±0.13 and 1.03±0.04 respectively (p value
Abstract: Freeze concentration freezes or crystallises the water
molecules out as ice crystals and leaves behind a highly concentrated
solution. In conventional suspension freeze concentration where ice
crystals formed as a suspension in the mother liquor, separation of
ice is difficult. The size of the ice crystals is still very limited which
will require usage of scraped surface heat exchangers, which is very
expensive and accounted for approximately 30% of the capital cost.
This research is conducted using a newer method of freeze
concentration, which is progressive freeze concentration. Ice crystals
were formed as a layer on the designed heat exchanger surface. In
this particular research, a helical structured copper crystallisation
chamber was designed and fabricated. The effect of two operating
conditions on the performance of the newly designed crystallisation
chamber was investigated, which are circulation flowrate and coolant
temperature. The performance of the design was evaluated by the
effective partition constant, K, calculated from the volume and
concentration of the solid and liquid phase. The system was also
monitored by a data acquisition tool in order to see the temperature
profile throughout the process. On completing the experimental
work, it was found that higher flowrate resulted in a lower K, which
translated into high efficiency. The efficiency is the highest at 1000
ml/min. It was also found that the process gives the highest
efficiency at a coolant temperature of -6 °C.
Abstract: Traditionally, Internet has provided best-effort service to every user regardless of its requirements. However, as Internet becomes universally available, users demand more bandwidth and applications require more and more resources, and interest has developed in having the Internet provide some degree of Quality of Service. Although QoS is an important issue, the question of how it will be brought into the Internet has not been solved yet. Researches, due to the rapid advances in technology are proposing new and more desirable capabilities for the next generation of IP infrastructures. But neither all applications demand the same amount of resources, nor all users are service providers. In this way, this paper is the first of a series of papers that presents an architecture as a first step to the optimization of QoS in the Internet environment as a solution to a SMSE's problem whose objective is to provide public service to internet with certain Quality of Service expectations. The service provides new business opportunities, but also presents new challenges. We have designed and implemented a scalable service framework that supports adaptive bandwidth based on user demands, and the billing based on usage and on QoS. The developed application has been evaluated and the results show that traffic limiting works at optimum and so it does exceeding bandwidth distribution. However, some considerations are done and currently research is under way in two basic areas: (i) development and testing new transfer protocols, and (ii) developing new strategies for traffic improvements based on service differentiation.
Abstract: In this paper, we propose an improvement of pattern
growth-based PrefixSpan algorithm, called I-PrefixSpan. The general idea of I-PrefixSpan is to use sufficient data structure for Seq-Tree
framework and separator database to reduce the execution time and
memory usage. Thus, with I-PrefixSpan there is no in-memory database stored after index set is constructed. The experimental result
shows that using Java 2, this method improves the speed of PrefixSpan up to almost two orders of magnitude as well as the memory usage to more than one order of magnitude.
Abstract: It is important to remove manganese from water
because of its effects on human and the environment. Human
activities are one of the biggest contributors for excessive manganese
concentration in the environment. The proposed method to remove
manganese in aqueous solution by using adsorption as in carbon
nanotubes (CNT) at different parameters: The parameters are CNT
dosage, pH, agitation speed and contact time. Different pHs are pH
6.0, pH 6.5, pH 7.0, pH 7.5 and pH 8.0, CNT dosages are 5mg,
6.25mg, 7.5mg, 8.75mg or 10mg, contact time are 10 min, 32.5 min,
55 min, 87.5 min and 120 min while the agitation speeds are 100rpm,
150rpm, 200rpm, 250rpm and 300rpm. The parameters chosen for
experiments are based on experimental design done by using Central
Composite Design, Design Expert 6.0 with 4 parameters, 5 levels and
2 replications. Based on the results, condition set at pH 7.0, agitation
speed of 300 rpm, 7.5mg and contact time 55 minutes gives the
highest removal with 75.5%. From ANOVA analysis in Design
Expert 6.0, the residual concentration will be very much affected by
pH and CNT dosage. Initial manganese concentration is 1.2mg/L
while the lowest residual concentration achieved is 0.294mg/L,
which almost satisfy DOE Malaysia Standard B requirement.
Therefore, further experiments must be done to remove manganese
from model water to the required standard (0.2 mg/L) with the initial
concentration set to 0.294 mg/L.
Abstract: Mobile ad hoc network is a collection of mobile
nodes communicating through wireless channels without any existing
network infrastructure or centralized administration. Because of the
limited transmission range of wireless network interfaces, multiple
"hops" may be needed to exchange data across the network. In order
to facilitate communication within the network, a routing protocol is
used to discover routes between nodes. The primary goal of such an
ad hoc network routing protocol is correct and efficient route
establishment between a pair of nodes so that messages may be
delivered in a timely manner. Route construction should be done
with a minimum of overhead and bandwidth consumption. This paper
examines two routing protocols for mobile ad hoc networks– the
Destination Sequenced Distance Vector (DSDV), the table- driven
protocol and the Ad hoc On- Demand Distance Vector routing
(AODV), an On –Demand protocol and evaluates both protocols
based on packet delivery fraction, normalized routing load, average
delay and throughput while varying number of nodes, speed and
pause time.
Abstract: Providing authentication for the messages exchanged
between group members in addition to confidentiality is an important
issue in Secure Group communication. We develop a protocol for
Secure Authentic Communication where we address authentication
for the group communication scheme proposed by Blundo et al.
which only provides confidentiality. Authentication scheme used is a
multiparty authentication scheme which allows all the users in the
system to send and receive messages simultaneously. Our scheme is
secure against colluding malicious parties numbering fewer than k.
Abstract: We introduce an extended resource leveling model that abstracts real life projects that consider specific work ranges for each resource. Contrary to traditional resource leveling problems this model considers scarce resources and multiple objectives: the minimization of the project makespan and the leveling of each resource usage over time. We formulate this model as a multiobjective optimization problem and we propose a multiobjective genetic algorithm-based solver to optimize it. This solver consists in a two-stage process: a main stage where we obtain non-dominated solutions for all the objectives, and a postprocessing stage where we seek to specifically improve the resource leveling of these solutions. We propose an intelligent encoding for the solver that allows including domain specific knowledge in the solving mechanism. The chosen encoding proves to be effective to solve leveling problems with scarce resources and multiple objectives. The outcome of the proposed solvers represent optimized trade-offs (alternatives) that can be later evaluated by a decision maker, this multi-solution approach represents an advantage over the traditional single solution approach. We compare the proposed solver with state-of-art resource leveling methods and we report competitive and performing results.
Abstract: True stress-strain curve of railhead steel is required to
investigate the behaviour of railhead under wheel loading through elasto-plastic Finite Element (FE) analysis. To reduce the rate of wear, the railhead material is hardened through annealing and
quenching. The Australian standard rail sections are not fully hardened and hence suffer from non-uniform distribution of the
material property; usage of average properties in the FE modelling can potentially induce error in the predicted plastic strains. Coupons
obtained at varying depths of the railhead were, therefore, tested under axial tension and the strains were measured using strain gauges as well as an image analysis technique, known as the Particle Image Velocimetry (PIV). The head hardened steel exhibit existence of three distinct zones of yield strength; the yield strength as the ratio of the average yield strength provided in the standard (σyr=780MPa) and
the corresponding depth as the ratio of the head hardened zone along
the axis of symmetry are as follows: (1.17 σyr, 20%), (1.06 σyr, 20%-80%) and (0.71 σyr, > 80%). The stress-strain curves exhibit limited plastic zone with fracture occurring at strain less than 0.1.
Abstract: Today-s children, who are born into a more colorful,
more creative, more abstract and more accessible communication
environment than their ancestors as a result of dizzying advances in
technology, have an interesting capacity to perceive and make sense
of the world. Millennium children, who live in an environment where
all kinds of efforts by marketing communication are more intensive
than ever are, from their early childhood on, subject to all kinds of
persuasive messages. As regards advertising communication, it
outperforms all the other marketing communication efforts in
creating little consumer individuals and, as a result of processing of
codes and signs, plays a significant part in building a world of seeing,
thinking and understanding for children. Children who are raised with
metaphorical expressions such as tales and riddles also meet that fast
and effective meaning communication in advertisements.
Children-s perception of metaphors, which help grasp the “product
and its promise" both verbally and visually and facilitate association
between them is the subject of this study. Stimulating and activating
imagination, metaphors have unique advantages in promoting the
product and its promise especially in regard to print advertisements,
which have certain limitations. This study deals comparatively with
both literal and metaphoric versions of print advertisements
belonging to various product groups and attempts to discover to what
extent advertisements are liked, recalled, perceived and are
persuasive. The sample group of the study, which was conducted in
two elementary schools situated in areas that had different socioeconomic
features, consisted of children aged 12.
Abstract: In this paper, we describe a rule-based message passing method to support developing collaborative applications, in which multiple users share resources in distributed environments. Message communications of applications in collaborative environments tend to be very complex because of the necessity to manage context situations such as sharing events, access controlling of users, and network places. In this paper, we propose a message communications method based on unification of artificial intelligence and logic programming for defining rules of such context information in a procedural object-oriented programming language. We also present an implementation of the method as java classes.
Abstract: Spam mails are unwanted mails sent to large number
of users. Spam mails not only consume the network resources, but
cause security threats as well. This paper proposes an efficient
technique to detect, and to prevent spam mail in the sender side rather
than the receiver side. This technique is based on a counter set on the
sender server. When a mail is transmitted to the server, the mail server
checks the number of the recipients based on its counter policy. The
counter policy performed by the mail server is based on some
pre-defined criteria. When the number of recipients exceeds the
counter policy, the mail server discontinues the rest of the process, and
sends a failure mail to sender of the mail; otherwise the mail is
transmitted through the network. By using this technique, the usage of
network resources such as bandwidth, and memory is preserved. The
simulation results in real network show that when the counter is set on
the sender side, the time required for spam mail detection is 100 times
faster than the time the counter is set on the receiver side, and the
network resources are preserved largely compared with other
anti-spam mail techniques in the receiver side.
Abstract: Recently, a vehicular ad-hoc networks(VANETs) for
Intelligent Transport System(ITS) have become able safety and convenience services surpassing the simple services such as
an electronic toll collection system. To provide the proper services,
VANET needs infrastructure over the country infrastructure. Thus, we have to spend a huge sum of
human resources. In this reason, several studies have been made on the
usage of cellular networks instead of new protocols
this study is to assess a performance evaluation of the
cellular network for VANET. In this paper, the result of a
for the suitability of cellular networks for VANET
experiment, The LTE(Long Term Evolution) of cellular networks found to be most suitable among the others cellular networks
Abstract: Protein-protein interactions (PPI) play a crucial role in many biological processes such as cell signalling, transcription, translation, replication, signal transduction, and drug targeting, etc. Structural information about protein-protein interaction is essential for understanding the molecular mechanisms of these processes. Structures of protein-protein complexes are still difficult to obtain by biophysical methods such as NMR and X-ray crystallography, and therefore protein-protein docking computation is considered an important approach for understanding protein-protein interactions. However, reliable prediction of the protein-protein complexes is still under way. In the past decades, several grid-based docking algorithms based on the Katchalski-Katzir scoring scheme were developed, e.g., FTDock, ZDOCK, HADDOCK, RosettaDock, HEX, etc. However, the success rate of protein-protein docking prediction is still far from ideal. In this work, we first propose a more practical measure for evaluating the success of protein-protein docking predictions,the rate of first success (RFS), which is similar to the concept of mean first passage time (MFPT). Accordingly, we have assessed the ZDOCK bound and unbound benchmarks 2.0 and 3.0. We also createda new benchmark set for protein-protein docking predictions, in which the complexes have experimentally determined binding affinity data. We performed free energy calculation based on the solution of non-linear Poisson-Boltzmann equation (nlPBE) to improve the binding mode prediction. We used the well-studied thebarnase-barstarsystem to validate the parameters for free energy calculations. Besides,thenlPBE-based free energy calculations were conducted for the badly predicted cases by ZDOCK and ZRANK. We found that direct molecular mechanics energetics cannot be used to discriminate the native binding pose from the decoys.Our results indicate that nlPBE-based calculations appeared to be one of the promising approaches for improving the success rate of binding pose predictions.
Abstract: Digital signature is a useful primitive to attain the integrity and authenticity in various wire or wireless communications. Proxy signature is one type of the digital signatures. It helps the proxy signer to sign messages on behalf of the original signer. It is very useful when the original signer (e.g. the president of a company) is not available to sign a specific document. If the original signer can not forge valid proxy signatures through impersonating the proxy signer, it will be robust in a virtual environment; thus the original signer can not shift any illegal action initiated by herself to the proxy signer. In this paper, we propose a new proxy signature scheme. The new scheme can prevent the original signer from impersonating the proxy signer to sign messages. The proposed scheme is based on the regular ElGamal signature. In addition, the fair privacy of the proxy signer is maintained. That means, the privacy of the proxy signer is preserved; and the privacy can be revealed when it is necessary.
Abstract: In a 10-week (May – August, 2008) Phase I trial, 840, 1+ rainbow trout, Oncorhynchus mykiss, received a commercial oral immunomodulator, Fin Immune™, at four different dosages (0, 10, 20 and 30 mg g-1) to evaluate immune response and growth. The overall objective of was to determine an optimal dosage of this product for rainbow trout that provides enhanced immunity with maximal growth and health. Biweekly blood samples were taken from 10 randomly selected fish in each tank (30 samples per treatment) to evaluate the duration of enhanced immunity conferred by Fin-Immune™. The immunological assessment included serum white blood cell (lymphocyte, neutrophil) densities and blood hematocrit (packed cell volume %). Of these three variables, only lymphocyte density increased significantly among trout fed Fin- Immune™ at 20 and 30 mg g-1 which peaked at week 6. At week 7, all trout were switched to regular feed (lacking Fin-Immune™) and by week 10, lymphocyte levels decreased among all levels but were still greater than at week 0. There was growth impairment at the highest dose of Fin-Immune™ tested (30 mg g-1) which can be associated with a physiological compensatory mechanism due to a dose-specific threshold level. Thus, our main objective of this Phase I study was achieved, the 20 mg g-1 dose of Fin-Immune™ should be the most efficacious (of those we tested) to use for a Phase II disease challenge trial.
Abstract: P2P Networks are highly dynamic structures since
their nodes – peer users keep joining and leaving continuously. In the
paper, we study the effects of network change rates on query routing
efficiency. First we describe some background and an abstract system
model. The chosen routing technique makes use of cached metadata
from previous answer messages and also employs a mechanism for
broken path detection and metadata maintenance. Several metrics are
used to show that the protocol behaves quite well even with high rate
of node departures, but above a certain threshold it literally breaks
down and exhibits considerable efficiency degradation.
Abstract: In this paper we describes the authentication for DHCP
(Dynamic Host Configuration Protocol) message which provides the
efficient key management and reduces the danger replay attack without
an additional packet for a replay attack. And the authentication for
DHCP message supports mutual authentication and provides both
entity authentication and message authentication. We applied the
authentication for DHCP message to the home network environments
and tested through a home gateway.
Abstract: Quantum cryptography offers a way of key agreement,
which is unbreakable by any external adversary. Authentication is
of crucial importance, as perfect secrecy is worthless if the identity
of the addressee cannot be ensured before sending important information.
Message authentication has been studied thoroughly, but no
approach seems to be able to explicitly counter meet-in-the-middle
impersonation attacks. The goal of this paper is the development of
an authentication scheme being resistant against active adversaries
controlling the communication channel. The scheme is built on top
of a key-establishment protocol and is unconditionally secure if built
upon quantum cryptographic key exchange. In general, the security
is the same as for the key-agreement protocol lying underneath.
Abstract: Currently, most of distance learning courses can only
deliver standard material to students. Students receive course content
passively which leads to the neglect of the goal of education – “to suit
the teaching to the ability of students". Providing appropriate course
content according to students- ability is the main goal of this paper.
Except offering a series of conventional learning services, abundant
information available, and instant message delivery, a complete online
learning environment should be able to distinguish between students-
ability and provide learning courses that best suit their ability.
However, if a distance learning site contains well-designed course
content and design but fails to provide adaptive courses, students will
gradually loss their interests and confidence in learning and result in
ineffective learning or discontinued learning. In this paper, an
intelligent tutoring system is proposed and it consists of several
modules working cooperatively in order to build an adaptive learning
environment for distance education. The operation of the system is
based on the result of Self-Organizing Map (SOM) to divide students
into different groups according to their learning ability and learning
interests and then provide them with suitable course content.
Accordingly, the problem of information overload and internet traffic
problem can be solved because the amount of traffic accessing the
same content is reduced.