International Journal of Biological, Life and Agricultural Sciences

Effect of Synthetic Queen Mandibular Pheromone on Pollination of Cotton by Honey Bees, Apis mellifera

Year: 2013 Volume: 7 Issue: 12 1135 - 1139 Pages

Abstract: The effectiveness of a commercial bee attractant, synthetic honey bee queen mandibular pheromone (Fruit Boost®) for enhancing pollination of Gossypium hirsutum was evaluated in a transgenic (Bt) cotton crop. The study assessed the number of bee visitations to blossoms of plants treated with Fruit Boost® as well, as effects on fruit set, yield, and lint quality. Bee activity on plots sprayed with pheromone concentrations of 50 and 500 queen equivalents (QEQ) /ha did not differ significantly from water-only control, on the day of application or the subsequent day. Application of the pheromone did not increase fruit set, yield, or lint quality. Two consecutive pheromone applications, applied two days apart, were not significantly different from a single application for any parameter.

Relative Suitability Evaluation of Two Methods of Particle-Size Analysis for Selected Soils of Sudan Savanna of Nigeria

Year: 2013 Volume: 7 Issue: 1 94 - 97 Pages

Abstract: The two widely used methods base on the sedimentation principle (Bouyoucos hydrometer and International pipette) for particle-size analysis were comparatively evaluated on soils collected from various locations in Sudan savanna of Nigeria particularly from Sokoto and Zamfara States. The hydrometer method under-estimated the silt and over-estimated the clay content. Also, the hydrometer reading proved difficult and tended to submerge when floated for clay reading in the suspension of very sandy soils (900g kg-1 sand). Furthermore, the results from the two methods were validated by subjecting the data to USDA soil textural triangle to determine their textural class names. The outcome was that 91.67 % of the experimental soils retained the same textural class names irrespective of the method. Thus, Bouyoucos hydrometer method may conveniently find a place in routine work in view of its simplicity, rapidity, and strong correlation with the pipette method.

Study of the Antimicrobial Activity of Aminoreductone against Pathogenic Bacteria in Comparison with Other Antibiotics

Year: 2013 Volume: 7 Issue: 12 1131 - 1134 Pages

Abstract: Antimicrobial activities of aminoreductone (AR), a product formed in the initial stage of Maillard reaction, were screened against pathogenic bacteria. A significant growth inhibition of AR against all 7 isolates (Staphylococcus aureus ATCC® 25923™, Salmonella typhimurium ATCC® 14028™, Bacillus cereus ATCC® 13061™, Bacillus subtilis ATCC® 11774™, Escherichia coli ATCC® 25922™, Enterococcus faecalis ATCC® 29212™, Listeria innocua ATCC® 33090™) were observed by the standard disc diffusion methods. The inhibition zone for each isolate by AR (2.5 mg) ranged from 15±0mm to 28.3±0.4mm in diameter. The minimum inhibitory concentration (MIC) of AR ranging from 20mM to 26mM was proven in the 7 isolates tested. AR also showed the similar effect of growth inhibition in comparison with antibiotics frequently used for the treatment of infections bacteria, such as amikacin, ciprofloxacin, meropennem and levofloxacin. The results indicated that foods containing AR are valuable sources of bioactive compounds towards pathogenic bacteria.

Effects of Hypoxic Duration at Different Growth Stages on Yield Potential of Waxy Corn (Zea mays L.)

Year: 2013 Volume: 7 Issue: 12 1128 - 1130 Pages

Abstract: Hypoxia has negative effects on growth and crop yield, its severity is so varied depending on crop growth stages, duration of hypoxia and crop species. The objective was to evaluate the sensitive growth stage and the duration of hypoxia negatively affecting growth and yield of waxy corn. Pot experiment was conducted using a split plot in randomized complete block with 3 growth stages: V3 (3-4 true leaves), V7 (7-8 true leaves) and R1 (silking stage), and 3 hypoxic durations: 6, 9 and 12 days, in an open –ended outdoor greenhouse during January to March 2013. The results revealed that different growth stages had significantly (p < 0.5) different responses to hypoxia, seeing that the sensitive growth stage affecting plant height, yield and yield components was mostly detected in V7 growth stage whereas leaf greenness and days to silking were sensitive to hypoxia at R1 growth stage. Different hypoxic durations significantly affected yield and yield components, hypoxic duration of 12 days showed the most negative effect greater than the others. In this present study, it can be concluded that waxy corn plants were waterlogged at V7 growth stage for 12 days had the most negative effect on yield and yield components.

Investigation the Difference of Several Hormones Correlated to Reproduction between Infertile and Fertile Dairy Cows

Year: 2013 Volume: 7 Issue: 12 1124 - 1127 Pages

Abstract: The object of this study was to investigate several hormones correlated to the reproduction and inhibin A, inhibin B and NO levels in the infertile dairy cows as attempt to illustrate the physiological causes of dairy cows infertility. 40 Holstein cow (21 infertile and 19 fertile) were used at estrous phase of the cycle, Hormones FSH, LH, E2, Testosterone, were measured using ELISA method. inhibin A and B also estimated by ELISA method, Nitric oxide was measured by Greiss reagent method. The results showed different concentrations of the hormone in which FSH illustrated significantly higher concentration in the infertile cows than fertile cows (P

The Expression of a Novel Gene Encoding an Ankyrin-Repeat Protein, DRA1, is Regulated by Drought-Responsive Alternative Splicing

Year: 2013 Volume: 7 Issue: 12 1120 - 1123 Pages

Abstract: Drought stress is a critical environmental factor that adversely affects crop productivity and quality. Because of their immobile nature, plants have evolved mechanisms to sense and respond to drought stress. We identified a novel locus of Arabidopsis, designated DRA1 (drought responsive ankyrin1), whose disruption leads to increased drought-stress tolerance. DRA1 encodes a transmembrane protein with an ankyrin-repeat motif that has been implicated in diverse cellular processes such as signal transduction. RT-PCR analysis revealed that there were at least two splicing variants of DRA1 transcripts in wild-type plants. In response to drought stress, the levels of DRA1 transcripts retaining second and third introns were increased, whereas these introns were removed under unstressed conditions. These results suggest that DRA1 protein may negatively regulate plant drought tolerance and that the expression of DRA1is regulated in response to drought stress by alternative splicing.

Political Economy of Integrated Soil Fertility Management in the Okavango Delta, Botswana

Year: 2013 Volume: 7 Issue: 11 1073 - 1081 Pages

Abstract: Although many factors play a significant role in agricultural production and productivity, the importance of soil fertility cannot be underestimated. The extent to which small farmers are able to manage the fertility of their farmlands is crucial in agricultural development particularly in sub-Saharan Africa (SSA). This paper assesses the nutrient status of selected farmers’ fields in relation to how government policy addresses the allocation of and access to agricultural inputs (e.g. chemical fertilizers) in a unique social-ecological environment of the Okavango Delta in northern Botswana. It also analyses small farmers and soil scientists’ perceptions about the political economy of integrated soil fertility management (ISFM) in the area. A multi-stage sampling procedure was used to elicit quantitative and qualitative information from 228 farmers and 9 soil researchers through the use of interview schedules and questionnaires, respectively. Knowledge validation workshops and focus group discussions (FGDs) were also used to collect qualitative data from farmers. Thirty-three composite soil samples were collected from 30 farmers’ plots in three farming communities of Makalamabedi, Nokaneng and Mohembo for laboratory analysis. While meeting points exist, farmers and scientists have divergent perspectives on soil fertility management. Laboratory analysis carried out shows that most soils in the wetland and the adjoining dry-land/upland surroundings are low in essential nutrients as well as in cation exchange capacity (CEC). Although results suggest the identification and use of appropriate inorganic fertilizers, the low CEC is an indication that holistic cultural practices, which are beyond mere chemical fertilizations, are critical and more desirable for improved soil health and sustainable livelihoods in the area. Farmers’ age (t= -0.728; p≤0.10); their perceptions about the political economy (t = -0.485; p≤0.01) of ISFM; and their preference for the use of local knowledge in soil fertility management (t = -10.254; p≤0.01) had a significant relationship with how they perceived their involvement in the implementation of ISFM.

Solvent Effect on Antioxidant Activity and Total Phenolic Content of Betula alba and Convolvulus arvensi

Year: 2013 Volume: 7 Issue: 5 351 - 356 Pages

Abstract: The potential of using herbal Betula alba (BA) and Convolvulus arvensis (CA) as a natural antioxidant for food applications were investigated. Each plant extract was prepared by using pure ethanol, different concentration of ethanol aqueous solutions, including 50% and 75%, 50% methanol aqueous and water. Total phenolic content (TPC) was determined using Folin–Ciocalteau method and antioxidant activity were analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, trolox equivalent antioxidant capacity (TEAC), Oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) respectively. Ethanol extract of CA exhibited the highest TPC and antioxidant activity; however BA showed varies of antioxidant activity value in each assay. The BA and CA exhibit the potential sources of natural antioxidant for food commodities.

Toxic Effect of Sodium Nitrate on Germinating Seeds of Vigna radiata

Year: 2013 Volume: 7 Issue: 10 1002 - 1006 Pages

Authors:
Nilima D. Gajbhiye

Abstract: Sodium nitrate has been used industrially in a number of work fields ranging from agriculture to food industry. Sodium nitrate and nitrite are associated with a higher risk of cancer in human beings. In present study, the effect of sodium nitrate on germinating seeds was studied. Two different sets of ungerminated Vigna radiata seeds were taken. In one set Vigna radiata seeds were soaked in distilled water for 4 hours and they were allowed to germinate in distilled water (Control) and 0.1 to 1% and 10% concentrations of sodium nitrate (NaNo3). In soaked seed set, on 2nd day radical developed in control and 0.1 to 1% concentrations of sodium nitrate. Seeds size was enlarged in 1% and 10% concentrations of sodium nitrate. On 3rd day in 0.1% sodium nitrate length of the radicle was 7.5cm with one leaf let and control sample showed 9cm with one leaflet. On 5th day in 0.1% sodium nitrate length of the radicle was 10 cm with one leaf let and control sample showed 11.5cm with one leaflet. No radicle developed in 1 and 10% NaNo3 concentrations. On 10th day all plants including control were dead. More number of mitotic cells was observed in apical root meristems of control germinating seeds and less mitotic cells were observed in 0.1% NaNo3 germinating seeds. But cells were elongated in 0.9%NaNo3 concentration and particles are deposited in the cells and no mitotic cells were observed. In other sets, dry seeds were allowed to germinate in Distilled water (control) and in 0.1 to 1% and 10% concentrations of sodium nitrate. In dry seed set, on 2nd day radicle developed from control set. In 0.1 to 1% concentrations of sodium nitration seed enlarged in size but but not allowed germination. But in 10% NaNo3 seeds coat colour was changed from dark green to brown. On 3rd day the radicle was developed in 0.1% concentration of NaNo3. No growth of radicle was observed in 0.3 to 10% concentrations of NaNo3 but plumule was observed in control plant. Seed coat color was changed from dark green to brown in color in 1% and 10% NaNo3. On 5th day in control seeds the radicle growth was 11cm and 0.1% NaNo3 concentration was 1.3 cm. On 10th day all plants including control were dead. More number of mitotic cells was observed in apical root meristems of control germinating seeds and less mitotic cells were observed in 0.1% NaNo3 germinating seeds. At higher concentrations of NaNo3 allowed seed germination in soaked seeds but produced radicle decay. In comparison to it, in dry seed set, germination of seeds observed only in 0.1% NaNo3 concentration. The inhibitory effect of NaNo3 on seed germination is due to reduction of water imbibition and mitotic activity.

Survival Model for Partly Interval-Censored Data with Application to Anti D in Rhesus D Negative Studies

Year: 2013 Volume: 7 Issue: 5 347 - 350 Pages

Abstract: This paper discusses regression analysis of partly interval-censored failure time data, which is occur in many fields including demographical, epidemiological, financial, medical and sociological studies. For the problem, we focus on the situation where the survival time of interest can be described by the additive hazards model in the present of partly interval-censored. A major advantage of the approach is its simplicity and it can be easily implemented by using R software. Simulation studies are conducted which indicate that the approach performs well for practical situations and comparable to the existing methods. The methodology is applied to a set of partly interval-censored failure time data arising from anti D in Rhesus D negative studies.

Intertidal Fixed Stake Net Trap (Hadrah) Fishery in Kuwait: Distribution, Catch Rate and Species Composition

Year: 2013 Volume: 7 Issue: 12 1114 - 1119 Pages

Abstract: Intertidal fixed stake net trap (Hadrah) is one of the oldest fishing gears used throughout the Arabian Gulf countries since the 1800s and also one of most the efficient methods of capturing fish from the intertidal area. This study describes the hadrah fishery in Kuwait. From October 2001 to December 2002, more than 37,372 specimens representing 95 species (89 fish, 2 mollusks and 4 crustaceans) were measured from hadrah, located in three different areas along Kuwait's coast. In Kuwait Bay, catch rates averaged 62 kg/sir-day (from 14 kg/sir-day in February to 160 kg/sir-day in October 2002). Commercial species accounted for 41% of the catches. Catches from Failakah Island averaged 96 kg/sir-day from June to September, with 61% of the catch being commercial species. In the southern area, catches averaged only 32 kg/sir-day and only 34% were commercially important. Forty percent of the hadrah catches were juveniles, which shows that Kuwait’s shallow intertidal waters, particularly in Kuwait Bay, served as prime nursery habitat,. To maintain ecosystem biodiversity and recruitment success of the fishes, we recommended that all hadrah should be removed from Kuwait Bay. In the future, removal of hadrah from other locations should be considered.

Use of Plant Antimicrobials for Food Preservation

Year: 2013 Volume: 7 Issue: 12 1110 - 1113 Pages

Abstract: Spoilage occurs in plant produce due to the action of field and storage microorganisms. The conditions of storage can also cause physiological spoilage. Various methods exist to ensure that these food substances maintain their quality long after harvesting. However, many of these methods either fail to keep the plant for the required period or predispose the plant to other spoilage risks. The major shortcoming posed by the use of many antimicrobials is the chemical residues it deposits in the food substance. The use of plants in preservation has been in use for a long period, though little understood then, it served its purposes. A better understanding of the roles of these plant parts in increasing the shelf life of farm produce has helped in the creation of more effective and safer means of pest and microbial control. This can be extended to plants that have not been used for these purposes initially. Microbial sources should also be investigated as these have provided cheaper sources of secondary metabolites.

The Potency of Sandfish (Holothuria scraba) as a Source of Natural Aphrodisiacs

Year: 2013 Volume: 7 Issue: 5 340 - 346 Pages

Abstract: Sandfish is one of marine biota that has a biomedicine (bioactive compound) potency. People in Gorontalo Province, Indonesia, have been sandfish as an aphrodisiac for men as it is believed that sandfish has a steroid hormone potency. This research aims at studying using the steroid hormone potency from every fraction of sandfish (meat and innards) and its activity of male reproduction (rooster) as an aphrodisiac. Steroid extraction was done using Touchstone and Kasparow method, and then it was utilized to study the effectiveness of bioassay of rooster. This research had five treatments and was done in complete randomized design. Based on Lieberman-Burchard and bioassay test, the author found that sandfish extract contains steroid hormone. Sandfish extract was able to enrich testosterone and cholesterol concentration in blood serum; fastening secondary reproduction characteristics of the rooster, and increasing growth as well as improving rooster’s comb. Therefore, sandfish steroid is potential to be used as an aphrodisiac for men.

Effect of Organic-waste Compost Addition on Leaching of Mineral Nitrogen from Arable Land and Plant Production

Year: 2013 Volume: 7 Issue: 6 463 - 468 Pages

Abstract: Application of compost in agriculture is very desirable worldwide. In the Czech Republic, compost is the most often used to improve soil structure and increase the content of soil organic matter, but the effects of compost addition on the fate of mineral nitrogen are only scarcely described. This paper deals with possibility of using combined application of compost, mineral and organic fertilizers to reduce the leaching of mineral nitrogen from arable land. To demonstrate the effect of compost addition on leaching of mineral nitrogen, we performed the pot experiment. As a model crop, Lactuca sativa L. was used and cultivated for 35 days in climate chamber in thoroughly homogenized arable soil. Ten variants of the experiment were prepared; two control variants (pure arable soil and arable soil with added compost), four variants with different doses of mineral and organic fertilizers and four variants of the same doses of mineral and organic fertilizers with the addition of compos. The highest decrease of mineral nitrogen leaching was observed by the simultaneous applications of soluble humic substances and compost to soil samples, about 417% in comparison with the control variant. Application of these organic compounds also supported microbial activity and nitrogen immobilization documented by the highest soil respiration and by the highest value of the index of nitrogen availability. The production of plant biomass after this application was not the highest due to microbial competition for the nutrients in soil, but was 24% higher in comparison with the control variant. To support these promising results the experiment should be repeated in field conditions.

Investigation of the Emulsifying Properties of Bambara Groundnut Flour and Starch

Year: 2013 Volume: 7 Issue: 11 1064 - 1072 Pages

Abstract: The current desire in food and industrial emulsification is the use of natural emulsifiers. Bambara groundnut flour (BGNF) and its starch (BGNS) will serve both emulsifying and nutritional purposes if found suitable. This current study was aimed at investigating the emulsifying properties of BGNF/BGNS. BGNS was extracted from the BGNF. Emulsions were prepared using a wide range of flour-oil-water and starch-oil-water composition as generated through the application of Response Surface (D-optimal) design. Prepared emulsions were investigated for stability to creaming/sedimentation (using the kinetic information from turbiscan) and flocculation/coalescence (by monitoring the droplet diameter growth using optical microscope) over 5 days. The most stable emulsions (one BGNF-stabilized and the other BGNS-stabilized) were determined. The optimal emulsifier/oil composition was 9g/39g for BGNF and 5g/30g for BGNS. The two emulsions had only 30% and 50% growth in oil droplet diameter respectively by day 5, compared to over 3000% in the unstable ones. The BGNF-stabilized emulsions were more stable than the BGNS-stabilized ones. Emulsions were successfully stabilized with BGNF and BGNS.

Consumption Pattern and Dietary Practices of Pregnant Women in Odeda Local Government Area of Ogun State

Year: 2013 Volume: 7 Issue: 11 1059 - 1063 Pages

Authors:
Ademuyiwa
M. O.
Sanni
S. A.

Abstract: The importance of maternal nutritional practices during pregnancy cannot be overemphasized. This paper assessed the consumption pattern and dietary practices of 50 pregnant women selected using purposive sampling technique from three health care centres (Primary Health Care Centre, Obantoko; Primary Health Care Centre Alabata; and the General Hospital, Odeda) in Odeda Local Government Area of Ogun State, Nigeria. Structured questionnaire was used to elicit information on socioeconomic status, consumption pattern and dietary practices. Data were analyzed using the Statistical Package for Social Sciences (SPSS, 17). The results indicated that about 58% of the pregnant women were below the age of 30 while 42% were ages 28-40 years. Only 16% had tertiary education while (38%) had secondary education, 52% earn income through petty trading. On food intake, 52% got their energy source from rice on a daily basis, followed by pap (38%) and eko (34%). For protein intake, 36% consumed bean cake on a daily basis while 66% consumed moinmoin 2-3 times a week. Orange (48%) and Green Leafy vegetable (40%) accounted for the mostly consumed fruit and vegetable on daily basis. In terms of animal origin, fish (76%), meat (58%) and eggs (30%) were consumed daily, while chicken and snail were consumed occasionally by 54% and 42%, respectively. Forty-six percent (46%) of the pregnant women eat more than three times daily; while 60% of the women eat outside their homes with 42% respondents eat out lunch and only two percent least eaten out dinner. It is important to increase in awareness campaign to sensitize the pregnant women on the importance of good nutrition especially fruits, vegetables and dairy products.

Revitalisation of Indigenous Food in Africa through Print and Electronic Media

Year: 2013 Volume: 7 Issue: 11 1055 - 1058 Pages

Abstract: Language and culture are interwoven that they cannot be separated, for the knowledge of a language cannot be complete without having the culture of the language. Indigenous food is a cultural aspect of any language that is expected to be acquired by all the speakers of the language. Indigenous food is known to be vital right from early years, which is also attributed to the healthy living of the ancient people. However it is discovered that the indigenous food is almost being replaced by fast food products such as Indomie noodles, Spaghetti and Macaroni to the extent that majority of the young folks prefer the eating of the fast foods and cannot prepare the indigenous foods which are good for growth and healthy living of people. Therefore, there is need to revitalize and re-educate people on the indigenous food which is an aspect of inter-cultural education of any language to prevent it from being forgotten or neglected. African foods are many, but this study focused on Nigerian food using some Yoruba dishes as a case study. Examples of Yoruba dishes are pounded yam and melon with vegetable and dried fish soup, beans pudding (moin moin) and pap (eko), water yam pudding with fish and meat (ikokore) and many more. The ingredients needed for the preparation of these indigenous foods contain some basic food nutrients which will be analyzed and their nutritional importance to human bodies will also be discussed. The process of re- awakening the education of indigenous food to the present and up-coming generation should be via print and electronic media in form of advertisements on posters, billboards, calendars and in rhymes on television programs, radio presentations, video tapes and CD–ROM apart from classroom teaching and learning. Indigenous food is a panacea to healthy living and longevity, a prevention of diseases and a means of accelerated healing of the body through natural foods.

Recovering Taraxacum kok-saghyz Rodin. via Seed and Callus Culture

Year: 2014 Volume: 8 Issue: 4 351 - 353 Pages

Abstract: This experiment was performed to optimize the medium for tissue culture of Taraxacum kok-saghyz Rodin. Different tissue culture approaches such as shoot regeneration from seed, callus formation from leaf explants and plant regeneration from callus were investigated in this study. All the explants were cultured on MS basal medium supplemented with 20g/l sucrose, 7g/l agar and different plant growth regulators. Seeds of Taraxacum kok-saghyzwere cultured on media containing different levels of BA and 2,4-D (0.5, 1.0 and 3.0mg/L) to direct shoot regeneration study. Leaf explants were cultured in different combination of BA (at three levels: 0.5, 1.0 and 3.0mg/L) and zeatin (at two levels: 0.5 and 1.0mg/L) to examine callus formation. After the callus formation the formed calli were cultured on different combinations of BA and NAA for shoot regeneration. BA at three levels (0.5 and 1.0 and 3.0mg/L) and NAA at two levels (0.5 and 1.0mg/L) in all possible combinations were used for shoot regeneration from callus. The results showed that the treatment containing 1.0mg/L 2,4-D in combination with 1.0mg/L BA was found to be the best one for shoot regeneration from seeds. The treatment with 1.0mg/L BA in combination with 1.0mg/L zeatin were found to be suitable treatments for callus production from leaf explants, as well. Moreover, 0.5mg/L BA alone or in combination with 1.0mg/L NAA were found to be the best treatments for shoot regeneration from callus.

The Effects of Weather Anomalies on the Quantitative and Qualitative Parameters of Maize Hybrids of Different Genetic Traits in Hungary

Year: 2013 Volume: 7 Issue: 11 1051 - 1054 Pages

Abstract: Hybrid selection and the application of hybrid specific production technologies are important in terms of the increase of the yield and crop safety of maize. The main explanation for this is climate change, since weather extremes are going on and seem to accelerate in Hungary too. The biological bases, the selection of appropriate hybrids will be of greater importance in the future. The issue of the adaptability of hybrids will be considerably appreciated. Its good agronomical traits and stress bearing against climatic factors and agrotechnical elements (e.g. different types of herbicides) will be important. There have been examples of 3-4 consecutive droughty years in the past decades, e.g. 1992-1993-1994 or 2009-2011-2012, which made the results of crop production critical. Irrigation cannot be the solution for the problem since currently only the 2% of the arable land is irrigated. Temperatures exceeding the multi-year average are characteristic mainly to the July and August in Hungary, which significantly increase the soil surface evaporation, thus further enhance water shortage. In terms of the yield and crop safety of maize, the weather of these two months is crucial, since the extreme high temperature in July decreases the viability of the pollen and the pistil of maize, decreases the extent of fertilization and makes grain-filling tardy. Consequently, yield and crop safety decrease.

The Impact of Crop Rotation and N Fertilization on the Leaf Area Index, Leaf Disease and Yield of Winter Wheat

Year: 2013 Volume: 7 Issue: 11 1047 - 1050 Pages

Abstract: The research focused on the effects of previous cropping and fertilizers on the LAI, rhythm of the dry matter, leaf disease intensity and amount of yield. Long term field experiments’ results proved that the previous crop fundamentally determines size, rate and dynamics of the dry matter formation in the spring time vegetation period. The LAI index and crop results of winter wheat can be influenced mainly by raising the fertilizer amount. N fertilization has an outstanding role in the changes in leaf area index (LAI), weight of dry matter and yield of winter wheat. According to our results, the interaction effect of leaf area index, weight of dry matter and fertilization resulted in the maximum yield in biculture and triculture.