Abstract: Langmuir–Blodgett (LB) films of polyaniline (PANI) grown onto ITO coated glass substrates were utilized for the fabrication of Uric acid biosensor for efficient detection of uric acid by immobilizing Uricase via EDC–NHS coupling. The modified electrodes were characterized by atomic force microscopy (AFM). The response characteristics after immobilization of uricase were studied using cyclic voltammetry and electrochemical impedance spectroscopy techniques. The uricase/PANI/ITO/glass bioelectrode studied by CV and EIS techniques revealed detection of uric acid in a wide range of 0.05 mM to 1.0 mM, covering the physiological range in blood. A low Michaelis–Menten constant (Km) of 0.21 mM indicates the higher affinity of immobilized Uricase towards its analyte (uric acid). The fabricated uric acid biosensor based on PANI LB films exhibits excellent sensitivity of 0.21 mA/mM with a response time of 4 s, good reproducibility, long shelf life (8 weeks) and high selectivity.
Abstract: Indium-tin oxide films are deposited by low plasma
temperature RF sputtering on highly flexible modification of glycol
polyethyleneterephtalate substrates. The produced layers are
characterized with transparency over 82 % and sheet resistance of
86.9 Ω/square. The film’s conductivity was further improved by
additional UV illumination from light source (365 nm), having power
of 250 W. The influence of the UV exposure dose on the structural
and electro-optical properties of ITO was investigated. It was
established that the optimum time of illumination is 10 minutes and
further UV treatment leads to polymer substrates degradation.
Structural and bonds type analysis show that at longer treatment
carbon atoms release and diffuse into ITO films, which worsen their
electrical behavior. For the optimum UV dose the minimum sheet
resistance was measured to be 19.2 Ω/square, and the maximum
transparency remained almost unchanged – above 82 %.
Abstract: The gustatory system allows animals to distinguish
varieties of food and affects greatly the consumption of food, hence
the health and growth of animals. In the current study, we
investigated the histogenesis of vallate papillae (VLP) in the rabbit
tongue using light and scanning electron microscopy. Samples were
obtained from rabbit embryos at the embryonic days 16-30 (E16-30),
and from newborns until maturity; 6 months. At E16, the first
primordia of vallate papillae were observed as small pits on the
surface epithelium of the tongue-s root. At E18, the caudal part was
prominent with loose mesenchymal tissue core; meanwhile the rostral
part of the papilla was remained as a thick mass of epithelial cells. At
E20-24, the side epithelium formed the primitive annular groove. At
E26, the primitive taste buds appeared only at the papillary surface
and reached their maturity by E28. The annular groove started to
appear at E26 became more defined at E28. The definitive vallate
papillae with substantial number of apparently mature taste buds
were observed by the end of the second week. We conclude that the
vallate papillae develop early and mature during the early postnatal
life.
Abstract: Bacterial molecular chaperone DnaK plays an essential role in protein folding, stress response and transmembrane targeting of proteins. DnaKs from many bacterial species, including Escherichia coli, Salmonella typhimurium and Haemophilus infleunzae are the molecular targets for the insect-derived antimicrobial peptide pyrrhocoricin. Pyrrhocoricin-like peptides bind in the substrate recognition tunnel. Despite the high degree of crossspecies sequence conservation in the substrate-binding tunnel, some bacteria are not sensitive to pyrrhocoricin. This work addresses the molecular mechanism of resistance of Helicobacter pylori DnaK to pyrrhocoricin. Homology modelling, structural and sequence analysis identify a single aminoacid substitution at the interface between the lid and the β-sandwich subdomains of the DnaK substrate-binding domain as the major determinant for its resistance.
Abstract: In the last decade, carbohydrates have attracted great
attention as renewable resources for the chemical industry.
Carbohydrates are abundantly found in nature in the form of
monomers, oligomers and polymers, or as components of
biopolymers and other naturally occurring substances. As natural
products, they play important roles in conferring certain physical,
chemical, and biological properties to their carrier molecules.The
synthesis of this particular carbohydrate glycomonomer is part of our
work to obtain biodegradable polymers. Our current paper describes
the synthesis and characterization of a novel carbohydrate
glycomonomer starting from D-glucose, in several synthesis steps,
that involve the protection/deprotection of the D-glucose ring via
acetylation, tritylation, then selective deprotection of the aromaticaliphatic
protective group, in order to obtain 1,2,3,4-tetra-O-acetyl-
6-O-allyl-β-D-glucopyranose. The glycomonomer was then obtained
by the allylation in drastic conditions of 1,2,3,4-tetra-O-acetyl-6-Oallyl-
β-D-glucopyranose with allylic alcohol in the presence of
stannic chloride, in methylene chloride, at room temperature. The
proposed structure of the glycomonomer, 2,3,4-tri-O-acetyl-1,6-di-
O-allyl-β-D-glucopyranose, was confirmed by FTIR, NMR and
HPLC-MS spectrometry. This glycomonomer will be further
submitted to copolymerization with certain acrylic or methacrylic
monomers in order to obtain competitive plastic materials for
applications in the biomedical field.
Abstract: The setting agent Ca(OH)2 for activation of slag
cement is used in the proportions of 0%, 2%, 4%, 6%, 8% and 10%
by various methods (substitution and addition by mass of slag
cement). The physical properties of slag cement activated by the
calcium hydroxide at anhydrous and hydrated states (fineness,
particle size distribution, consistency of the cement pastes and setting
times) were studied. The activation method by the mineral activator
of slag cement (latent hydraulicity) accelerates the hydration process
and reduces the setting times of the cement activated.
Abstract: Since IEC61850 substation communication standard represents the trend to develop new generations of Substation Automation System (SAS), many IED manufacturers pursue this technique and apply for KEMA. In order to put on the market to meet customer demand as fast as possible, manufacturers often apply their products only for basic environment standard certification but claim to conform to IEC61850 certification. Since verification institutes generally perform verification tests only on specific IEDs of the manufacturers, the interoperability between all certified IEDs cannot be guaranteed. Therefore the interoperability between IEDs from different manufacturers needs to be tested. Based upon the above reasons, this study applies the definitions of the information models, communication service, GOOSE functionality and Substation Configuration Language (SCL) of the IEC61850 to build the concept of communication protocols, and build the test environment. The procedures of the test of the data collection and exchange of the P2P communication mode and Client / Server communication mode in IEC61850 are outlined as follows. First, test the IED GOOSE messages communication capability from different manufacturers. Second, collect IED data from each IED with SCADA system and use HMI to display the SCADA platform. Finally, problems generally encountered in the test procedure are summarized.
Abstract: In this paper, a simple microfluidic device for monitoring algal cell behavior is proposed. An array of algal microwells is fabricated by PDMS soft-lithography using X-ray LIGA mold, placed on a glass substrate. Two layers of replicated PDMS and substrate are attached by oxygen plasma bonding, creating a microchannel for the microfluidic system. Algal cell are loaded into the microfluidic device, which provides positive charge on the bottom surface of wells. Algal cells, which are negative charged, can be attracted to the bottom of the wells via electrostatic interaction. By varying the concentration of algal cells in the loading suspension, it is possible to obtain wells with a single cell. Liquid medium for cells monitoring are flown continuously over the wells, providing nutrient and waste exchange between the well and the main flow. This device could lead to the uncovering of the quantitative biology of the algae, which is a key to effective and extensive algal utilizations in the field of biotechnology, food industry and bioenergy research and developments.
Abstract: In this manuscript, we discuss the problem of determining the optimum stratification of a study (or main) variable based on the auxiliary variable that follows a uniform distribution. If the stratification of survey variable is made using the auxiliary variable it may lead to substantial gains in precision of the estimates. This problem is formulated as a Nonlinear Programming Problem (NLPP), which turn out to multistage decision problem and is solved using dynamic programming technique.
Abstract: There are several approaches in trying to solve the
Quantitative 1Structure-Activity Relationship (QSAR) problem.
These approaches are based either on statistical methods or on
predictive data mining. Among the statistical methods, one should
consider regression analysis, pattern recognition (such as cluster
analysis, factor analysis and principal components analysis) or partial
least squares. Predictive data mining techniques use either neural
networks, or genetic programming, or neuro-fuzzy knowledge. These
approaches have a low explanatory capability or non at all. This
paper attempts to establish a new approach in solving QSAR
problems using descriptive data mining. This way, the relationship
between the chemical properties and the activity of a substance
would be comprehensibly modeled.
Abstract: The paper deals with the possibilities of modelling
vapour propagation of explosive substances in the FLUENT
software. With regard to very low tensions of explosive substance
vapours the experiment has been verified as exemplified by
mononitrotoluene. Either constant or time variable meteorological
conditions have been used for calculation. Further, it has been
verified that the eluent source may be time-dependent and may reflect
a real situation or the liberation rate may be constant. The execution
of the experiment as well as evaluation were clear and it could also
be used for modelling vapour and aerosol propagation of selected
explosive substances in the atmospheric boundary layer.
Abstract: This paper contributes to the literature concerning burden sharing. We provide a quantitative expression of the burden sharing behaviour of 174 states in the case of combating terrorist financing and address specific burden sharing issues in this context (i.e., weakest link; no substitutability). We conclude that advanced states have shown more effort to control terrorist financing than developing states. In this particular case, there is an incentive for advanced states to support developing states. Failing to do so will make the total financial system worse off.
Abstract: Vinegar or sour wine is a product of alcoholic and
subsequent acetous fermentation of sugary precursors derived from
several fruits or starchy substrates. This delicious food additive and
supplement contains not less than 4 grams of acetic acid in 100 cubic
centimeters at 20°C. Among the large number of bacteria that are
able to produce acetic acid, only few genera are used in vinegar
industry most significant of which are Acetobacter and
Gluconobacter. In this research we isolated and identified an
Acetobacter strain from Iranian apricot, a very delicious and sensitive
summer fruit to decay, we gathered from fruit's stores in Isfahan,
Iran. The main culture media we used were Carr, GYC, Frateur and
an industrial medium for vinegar production. We isolated this strain
using a novel miniature fermentor we made at Pars Yeema
Biotechnologists Co., Isfahan Science and Technology Town (ISTT),
Isfahan, Iran. The microscopic examinations of isolated strain from
Iranian apricot showed gram negative rods to cocobacilli. Their
catalase reaction was positive and oxidase reaction was negative and
could ferment ethanol to acetic acid. Also it showed an acceptable
growth in 5%, 7% and 9% ethanol concentrations at 30°C using
modified Carr media after 24, 48 and 96 hours incubation
respectively. According to its tolerance against high concentrations of
ethanol after four days incubation and its high acetic acid production,
8.53%, after 144 hours, this strain could be considered as a suitable
industrial strain for a production of a new type of vinegar, apricot
vinegar, with a new and delicious taste. In conclusion this is the first
report of isolation and identification of an Acetobacter strain from
Iranian apricot with a very good tolerance against high ethanol
concentrations as well as high acetic acid productivity in an
acceptable incubation period of time industrially. This strain could be
used in vinegar industry to convert apricot spoilage to a beneficiary
product and mentioned characteristics have made it as an amenable
strain in food and agricultural biotechnology.
Abstract: Knowledge is a key asset for any organisation to
sustain competitive advantages, but it is difficult to identify and
represent knowledge which is needed to perform activities in
business processes. The effective knowledge management and
support for relevant business activities definitely gives a huge impact
to the performance of the organisation as a whole. This is because
that knowledge have the functions of directing, coordinating and
controlling actions within business processes. The study has
introduced organisational morphology, a norm-based approach by
applying semiotic theories which emphasise on the representation of
knowledge in norms. This approach is concerned with the
identification of activities into three categories: substantive,
communication and control activities. All activities are directed by
norms; hence three types of norms exist; each is associated to a
category of activities. The paper describes the approach briefly and
illustrates the application of this approach through a case study of
academic activities in higher education institutions. The result of the
study shows that the approach provides an effective way to profile
business knowledge and the profile enables the understanding and
specification of business requirements of an organisation.
Abstract: This paper is concerned with the production of an Arabic word semantic similarity benchmark dataset. It is the first of its kind for Arabic which was particularly developed to assess the accuracy of word semantic similarity measurements. Semantic similarity is an essential component to numerous applications in fields such as natural language processing, artificial intelligence, linguistics, and psychology. Most of the reported work has been done for English. To the best of our knowledge, there is no word similarity measure developed specifically for Arabic. In this paper, an Arabic benchmark dataset of 70 word pairs is presented. New methods and best possible available techniques have been used in this study to produce the Arabic dataset. This includes selecting and creating materials, collecting human ratings from a representative sample of participants, and calculating the overall ratings. This dataset will make a substantial contribution to future work in the field of Arabic WSS and hopefully it will be considered as a reference basis from which to evaluate and compare different methodologies in the field.
Abstract: Image interpolation is a common problem in imaging applications. However, most interpolation algorithms in existence suffer visually to some extent the effects of blurred edges and jagged artifacts in the image. This paper presents an adaptive feature preserving bidirectional flow process, where an inverse diffusion is performed to enhance edges along the normal directions to the isophote lines (edges), while a normal diffusion is done to remove artifacts (''jaggies'') along the tangent directions. In order to preserve image features such as edges, angles and textures, the nonlinear diffusion coefficients are locally adjusted according to the first and second order directional derivatives of the image. Experimental results on synthetic images and nature images demonstrate that our interpolation algorithm substantially improves the subjective quality of the interpolated images over conventional interpolations.
Abstract: Creation of information society, or in other words, a
society based on knowledge, has wide consequences, both on
individual and complete society, and in general – on a economy of
one country. Development and implementation of ICT represents a
stimulant for economic growth. On individual level, knowledge,
skills and information gathered using ICT, are expanding individual
possibilities of persons, enabling them to have access to timely
sensitive information, such as market prices or investment
conditions, possibilities to access Government-s or private
development funds, etc. By doing so, productivity is increased both
on individual and national level and therefore social wellbeing in
general. In one word, creation of information society - a knowledge
society is happening.
This work will describe challenges and strategies that will follow
the development as well as obstacles in creating information society
– knowledge society in Montenegro.
Abstract: In order to characterize the soy protein hydrolysate obtained in this study, gel chromatography on Sephadex G-25 was used to perform the separation of the peptide mixture and electrophoresis in SDS-polyacrylamide gel has been employed. Protein hydrolysate gave high antioxidant activities, but didn't give any antimicrobial activities. The antioxidant activities of protein hydrolysate was in the same trend of peptide content which gave high antioxidant activities and high peptide content between fractions 15 to 50. With increasing peptide concentrations, the scavenging effect on DPPH radical increased until about 70%, thereafter reaching a plateau. In compare to different concentrations of BHA, which exhibited higher activity (90%), soybean protein hydrolysate exhibited high antioxidant activities (70%) at a concentration of 1.45 mg/ml at fraction 25. Electrophoresis analysis indicated that, low- MW hydrolysate fractions (F1) appeared, on average, to have higher DPPH scavenging activities than high-MW fractions. These results revealed that soybean peptides probably contain substances that were proton donors and could react with free radicals to convert them to stable diamagnetic molecules.
Abstract: In the present study, the oleaginous fungus
Mortierella alpina CBS 754.68 was screened for arachidonic
acidproduction using inexpensive agricultural by-products as
substrate. Four oilcakes were analysed to choose the best substrate
among them. Sunflower oilcake was the most effective substrate for
ARA production followed by soybean, colza and olive oilcakes. In
the next step, seven variables including substrate particle size,
moisture content, time, temperature, yeast extract supply, glucose
supply and glutamate supply were surveyed and effective variables
for ARA production were determined using a Plackett-Burman
screening design. Analysis results showed that time (12 days),
substrate particle size (1-1.4 mm) and temperature (20ºC) were the
most effective variables for the highest level of ARA production
respectively.
Abstract: In order to answer the general question: “What does a simple agent with a limited life-time require for constructing a useful representation of the environment?" we propose a robot platform including the simplest probabilistic sensory and motor layers. Then we use the platform as a test-bed for evaluation of the navigational capabilities of the robot with different “brains". We claim that a protocognitive behavior is not a consequence of highly sophisticated sensory–motor organs but instead emerges through an increment of the internal complexity and reutilization of the minimal sensory information. We show that the most fundamental robot element, the short-time memory, is essential in obstacle avoidance. However, in the simplest conditions of no obstacles the straightforward memoryless robot is usually superior. We also demonstrate how a low level action planning, involving essentially nonlinear dynamics, provides a considerable gain to the robot performance dynamically changing the robot strategy. Still, however, for very short life time the brainless robot is superior. Accordingly we suggest that small organisms (or agents) with short life-time does not require complex brains and even can benefit from simple brain-like (reflex) structures. To some extend this may mean that controlling blocks of modern robots are too complicated comparative to their life-time and mechanical abilities.