Histogenesis of Rabbit Vallate Papillae

The gustatory system allows animals to distinguish varieties of food and affects greatly the consumption of food, hence the health and growth of animals. In the current study, we investigated the histogenesis of vallate papillae (VLP) in the rabbit tongue using light and scanning electron microscopy. Samples were obtained from rabbit embryos at the embryonic days 16-30 (E16-30), and from newborns until maturity; 6 months. At E16, the first primordia of vallate papillae were observed as small pits on the surface epithelium of the tongue-s root. At E18, the caudal part was prominent with loose mesenchymal tissue core; meanwhile the rostral part of the papilla was remained as a thick mass of epithelial cells. At E20-24, the side epithelium formed the primitive annular groove. At E26, the primitive taste buds appeared only at the papillary surface and reached their maturity by E28. The annular groove started to appear at E26 became more defined at E28. The definitive vallate papillae with substantial number of apparently mature taste buds were observed by the end of the second week. We conclude that the vallate papillae develop early and mature during the early postnatal life.

Authors:

• Elnasharty M.
• El Sharaby A.
• Nor El-din A.

Keywords:

• Rabbit
• vallate papillae
• histogenesis
• taste buds.

References:

[1] Cheecke, P.R., (1986): Potentials of rabbit production in tropical and
sub tropical agricultural systems. J. Anim. Sci., 63(5): 1581-1586.
[2] Finzi, A. and Amici, A. (1991): Traditional and alternative rabbit
breeding systems for developing countries. Rivista di Agricultura
Subtropicale e Tropicale, 6 (1):103-125.
[3] Hosley, M. A and Oakley, B. (1987): Postnatal Development of the
Vallate Papilla and Taste Buds in Rats. The Anatomical Record,
218:216-222.
[4] Miller I. J. and Smith D. V. (1988): Proliferation of taste buds in the
foliate and vallate papillae of postnatal hamsters. Growth Dev. Aging,
52: 123-131.
[5] Witt M. and I. J. Miller (1992): Comparative lectin histochemistry on
taste buds in foliate, circumvallate and fungiform papillae of the
rabbit tongue. Histochemistry and Cell Biology, Volume 98, Number
3, 173-182.
[6] Kulawik, M. and Godynicki, S. (2008): Development dynamics of
vallate papillae in the rabbit (Oryctolagus Cuniculus F. Domestica)
Acta Sci. Pol., Medicina Veterinaria, 7(1): 3-9.
[7] De Lorenzo A.J. (1958): Electron microscopic observations on the
taste buds of the rabbit. J Biophys Biochem Cytol 4:143-150.
[8] Scalzi H.A. (1967): The cytoarchitecture of gustatory receptors from
the rabbit foliate papillae. Z Zeliforsch, 80: 413-435.
[9] Nonaka, K., Zheng, J. H. And Kobayashi, K. (2008): Comparative
morphological study on the lingual papillae and their connective
tissue cores in rabbits. Okajimas Folia Anat. Jpn., 85(2): 57-66.
[10] Bancroft, J. D. and Gamble, M. (2013): Theory and Practice of
Histological Techniques. Churchill Livingstone; 7th edition.
[11] Ferrell, F. (1984): Taste Bud Morphology in the Fetal and Neonatal
Dog. Neuroscience & Biobehavioral Reviews, 8:175-183.
[12] Jitpukdeebodintra, S.; Chai, Y. and Snead M. L. (2002):
Developmental patterning of the circumvallate papilla. Int. J. Dev.
Biol. 46: 755-763.
[13] Arey, L. B. (1974): Developmental Anatomy, A Textbook and
Laboratory Manual of Embryology. Philadelphia and London: W. B.
Saunders; 7th ed., ch. 13, pp. 232-236.
[14] De Iongh, R.U.; Lovicu, F.J.; Overbeek, P.A.; Schneider; M.D.; Joya,
J., Hardeman, E.D. and Mcavoy, J.W. (2001): Requirement for TGFβ
receptor signaling during terminal lens fibers differentiation.
Development, 128: 3995- 4010.
[15] Lee, M. J.; Kim, J. Y.; Lee, S. I.; Sasaki, H.; Lunny, D. P.; Lane, E.
B., and Jung, H. S. (2006): Association of Shh and Ptc with keratin
localization in the initiation of the formation of circumvallate papilla
and von Ebner-s gland. Cell Tissue Res., 325(2): 253-261.
[16] Tichy, F. and Cerny, H. (1987): The morphogenesis of circumvallate
papillae and differentiation of taste buds in sheep ontogeny. Acta Vet.
Brno., 56: 261-274.
[17] Tichy, F. (1993): The morphogenesis of circumvallate papillae and the
differentiation of taste buds in the cat in ontogeny. Acta Vet. Brno, 62:
19-26.
[18] Tichy, F. (1992): The morphogenesis of selected lingual papillae in
ovine and porcine foetuses observed by scanning electron microscopy.
Acta Vet. Brno, 81: 3-10.
[19] Mistretta, C.M. and Haus, L.F. (1996): Temporal and spatial patterns
of tenascin and laminin immunoreactivity suggest roles for
extracellular matrix in development of gustatory papillae and taste
buds. J Comp Neurol., 15; 364(3): 535-555.
[20] Doughbag, A.E. (1988): Electron microscopic studies on the
morphogenesis of the lingual gustatory papillae of camel (Camelus
dromedarius). II. Morphogenesis of the circumvallate papillae. Z.
microsk. Anat. Forsch. Leipzig, 102(2): 259-271.
[21] Bradley, R. M. and Stern, I. B. (1967): The development of the human
taste bud during the foetal period. J Anat, 101: 743-752.
[22] Farbman, A. I. (1965): Electron microscope study of the developing
taste bud in rat fungiform papilla. Dev. Biol., 11:110-135.
[23] El-sharaby A., K. Ueda, and S. Wakisaka (2004):
Immunohistochemical Distribution of Growth-Associated Protein 43
(GAP-43) in Developing Rat Nasoincisor Papilla. Anatomical Record
Part A 277A:370-383 (2004)
[24] Uchida, N.; Kanazawa, M.; Suzuki, Y. and Takeda, M. (2003):
Expression of BDNF and TrkB in mouse taste buds after denervation
and in circumvallate papillae during development. Arch Histo Cyto,
66:17-25.
[25] El-Sharaby, A. E. (2006): Comparative Morphological Studies of the
Circumvallate Papillae in Three Mammalian Species: Rat, Dog and
Sheep. 12th Sci. Cong., Fac. Vet. Med., Assiut Univ., Egypt (661-
677).
[26] Blakemore, C. and Cooper, G. F. (1970): Development of the brain
depends on visual environment. Nature, 228: 477-478.
[27] Mistretta, C. M. and Bradley, R. M. (1977): Taste in utero: theoretical
considerations. In: Taste and Development: The Genesis of Sweet
Preference, edited by J. M. Weiffenbach. Bethesda, MD: DHEW,
PHS, NIH, pp. 51-69.
[28] Kobayashi, K. and Wanichanon, C. (1992): Stereo architecture of the
connective tissue cores of the lingual papillae in the tree shrew. Anat
Embryol (Berl), 186:511-518.
[29] Silva, M.C.; Watanabe, I. and Kronka, M.C. (2002): Threedimensional
architecture of the connective tissue core and surface
structures of the lingual papillae in the rabbit. Histol Histopathol, 17:
455-461.
[30] Abd-Elnaiem, M. M. M.; Zayed, A. E. and leiser, R. (2002):
Morphological characteristics of the tongue and its papillae in the
donkey (Equusasinus): a light and scanning electron microscopical
study. Ann.Anat., 184(5): 473-80.
[31] Bargmann, W. (1981): Histologia y anatomia microscopica humana.
Expaxs, Barcelona.
[32] Nagato, T.; Ren, X.Z.; Toh, H. and Tandler, B. (1997): Ultrastructure
of Weber`s salivary glands of the root of the tongue in the rat. Anat.
Rec., 249(4): 435-440.
[33] Paliwal, A.; Srikantan, S.; De, P.K.; Hand, A.R. and
Redman,R.S.(2006):Histological and biochemical characterization of
von Ebner-s glands in the Syrian hamster; comparison with rat von
Ebner-s glands Biotechnic & Histochemistry, 81(4_6): 139-149.
[34] Smith, R.D. and Pinkstaff, C.A (1982): Histology and mucosubstance
histochemistry of the lingual salivary glands of Galago senegalensis.
In: The Lesser Bushbaby (Galago) as an Animal Model: Selected
Topics. D.E. Haines, ed. CRC Press, Boca Raton, 220-230.
[35] Nogueira, J.C., and de Carvalho A.D.V. (1973): Histochemistry of
the mucins in the posterior lingual salivary glands of some mammals.
Rev. Bras. Pesquisas Me'd. Biol., 6:267-274.
[36] Li, X. and Snyder, S.H. (1995): Molecular cloning of Ebnerin, a von
Ebner-s gland protein associated with taste buds. J. Biol. Chem.,
270:17674-17679.
[37] Triantafyllou, A.; Fletcher, D. and Scott, J. (2001): Histochemical
phenotypes of von Ebner-s gland of ferret and their functional
implications. The Histochemical Journal, 33: 173-181.
[38] Pedini, V.; Gargiulo, A.M. and Ceccarelli, P. (1997): Basic and lectin
histochemical characterization of bovine gustatory (von Ebner-s)
glands. Anat Histol Embryol, 26: 223-226.
[39] De Blas, C. and Wiseman, J. (2010): Nutrition of the Rabbit, 2nd
Edition. CABI International, pp. 19-38.