Abstract: A study was conducted to evaluate the effects of diets containing different levels of lysine and canola oil on growth performance and fatty acid composition of meat of broilers chicks. 240-day old Ross broiler chicks were used in a 3×2 factorial arrangement with canola oil (1, 3, and 5%) and lysine (recommended, and 25% more than recommended by Ross broiler manual) in completely randomized design with four replicates and 10 birds per each. The experimental diets were iso-caloric and iso-nitrogenous. Feed intake and body weight gain were recorded at the end of starter (10 d), grower (24 d) and finisher (42 d) periods, and feed conversion ratio was calculated. The results showed that the weight gain of chickens fed diets containing 5% canola oil were greater than those of birds fed on other diets (P
Abstract: In this study, the crude extracts of Virgularia gustavina were examined as antibacterial, antifungal and anti-inflammatory agent. To assess inflammation, Xylene was applied to the ear of mice. The mice of the experimental group were fed with doses of 10 mg/kg, 20 mg/kg, and 40 mg/kg of lipid extract of chloroform and hexane as a separate group and then statistical analysis was performed on the results. Chloroform and hexane extracts of sea pen have strong anti-inflammatory effects even at low doses which is probably due to 54% arachidonic acid. Antibacterial and antifungal effects of hexane and chloroform extracts were measured with MIC and MBC methods and it is shown that chloroform extract has best activity against Staphylococcus aureus on 125 µg/ml doze in MIC method.
Abstract: In the present study, the oleaginous fungus
Mortierella alpina CBS 754.68 was screened for arachidonic
acidproduction using inexpensive agricultural by-products as
substrate. Four oilcakes were analysed to choose the best substrate
among them. Sunflower oilcake was the most effective substrate for
ARA production followed by soybean, colza and olive oilcakes. In
the next step, seven variables including substrate particle size,
moisture content, time, temperature, yeast extract supply, glucose
supply and glutamate supply were surveyed and effective variables
for ARA production were determined using a Plackett-Burman
screening design. Analysis results showed that time (12 days),
substrate particle size (1-1.4 mm) and temperature (20ºC) were the
most effective variables for the highest level of ARA production
respectively.
Abstract: Microorganisms isolated from water and soil of
Kazakhstan to identify potential high-effective producers of the
arachidonic acid, exhibiting a wide range of physiological activity
and having practical applications were screened. Based on the results
of two independent tests (the test on the sensitivity of the growth
processes of microorganisms to acetylsalicylic acid - an irreversible
inhibitor of PGH-synthase involved in the metabolism of arachidonic
acid and its derivatives, the test for inhibition of peroxidase activity
of membrane-bounding fraction of PGH - synthase by acetylsalicylic
acid) were selected microbial cultures which are potential highproducer
of arachidonic acid. They are characterized by a stable
strong growth in the laboratory conditions. Identification of
microorganism cultures based on morphological, physiological,
biochemical and molecular genetic characteristics was performed.