Abstract: Aim of this work was to compare the efficacy of two
loading methods of proteins onto polymeric nanocarriers: adsorption
and encapsulation methods. Preliminary studies of protein loading
were done using Bovine Serum Albumin (BSA) as model protein.
Nanocarriers were prepared starting from polylactic co-glycolic acid
(PLGA) polymer; production methods used are two different variants
of emulsion evaporation method. Nanoparticles obtained were
analyzed in terms of dimensions by Dynamic Light Scattering and
Loading Efficiency of BSA by Bradford Assay. Loaded
nanoparticles were then submitted to in-vitro protein dissolution test
in order to study the effect of the delivery system on the release rate
of the protein.
Abstract: The Institute of Product Development is dealing
with the development, design and dimensioning of micro components
and systems as a member of the Collaborative Research
Centre 499 “Design, Production and Quality Assurance of
Molded micro components made of Metallic and Ceramic Materials".
Because of technological restrictions in the miniaturization
of conventional manufacturing techniques, shape and
material deviations cannot be scaled down in the same proportion
as the micro parts, rendering components with relatively
wide tolerance fields. Systems that include such components
should be designed with this particularity in mind, often requiring
large clearance. On the end, the output of such systems
results variable and prone to dynamical instability. To save
production time and resources, every study of these effects
should happen early in the product development process and
base on computer simulation to avoid costly prototypes. A
suitable method is proposed here and exemplary applied to a
micro technology demonstrator developed by the CRC499. It
consists of a one stage planetary gear train in a sun-planet-ring
configuration, with input through the sun gear and output
through the carrier. The simulation procedure relies on ordinary
Multi Body Simulation methods and subsequently adds
other techniques to further investigate details of the system-s
behavior and to predict its response. The selection of the relevant
parameters and output functions followed the engineering
standards for regular sized gear trains. The first step is to
quantify the variability and to reveal the most critical points of
the system, performed through a whole-mechanism Sensitivity
Analysis. Due to the lack of previous knowledge about the system-s
behavior, different DOE methods involving small and
large amount of experiments were selected to perform the SA.
In this particular case the parameter space can be divided into
two well defined groups, one of them containing the gear-s profile
information and the other the components- spatial location.
This has been exploited to explore the different DOE techniques
more promptly. A reduced set of parameters is derived for
further investigation and to feed the final optimization process,
whether as optimization parameters or as external perturbation
collective. The 10 most relevant perturbation factors and 4 to 6
prospective variable parameters are considered in a new, simplified
model. All of the parameters are affected by the mentioned
production variability. The objective functions of interest
are based on scalar output-s variability measures, so the
problem becomes an optimization under robustness and reliability constrains. The study shows an initial step on the development
path of a method to design and optimize complex micro
mechanisms composed of wide tolerated elements accounting
for the robustness and reliability of the systems- output.
Abstract: This report aims to utilize existing and future Multiple-Input Multiple-Output Orthogonal Frequency Division Multiplexing Wireless Local Area Network (MIMO-OFDM WLAN) systems characteristics–such as multiple subcarriers, multiple antennas, and channel estimation characteristics–for indoor location estimation systems based on the Direction of Arrival (DOA) and Radio Signal Strength Indication (RSSI) methods. Hybrid of DOA-RSSI methods also evaluated. In the experimental data result, we show that location estimation accuracy performances can be increased by minimizing the multipath fading effect. This is done using multiple subcarrier frequencies over wideband frequencies to estimate one location. The proposed methods are analyzed in both a wide indoor environment and a typical room-sized office. In the experiments, WLAN terminal locations are estimated by measuring multiple subcarriers from arrays of three dipole antennas of access points (AP). This research demonstrates highly accurate, robust and hardware-free add-on software for indoor location estimations based on a MIMO-OFDM WLAN system.
Abstract: We propose a downlink multiple-input multipleoutput
(MIMO) multi-carrier code division multiple access (MCCDMA)
system with adaptive beamforming algorithm for smart
antennas. The algorithm used in this paper is based on the Least
Mean Square (LMS), with pilot channel estimation (PCE) and the
zero forcing equalizer (ZFE) in the receiver, requiring reference
signal and no knowledge channel. MC-CDMA is studied in a
multiple antenna context in order to efficiently exploit robustness
against multipath effects and multi-user flexibility of MC-CDMA and
channel diversity offered by MIMO systems for radio mobile
channels. Computer simulations, considering multi-path Rayleigh
Fading Channel, interference inter symbol and interference are
presented to verify the performance. Simulation results show that the
scheme achieves good performance in a multi-user system.
Abstract: The removal of hydrogen sulphide is required for reasons of health, odour problems, safety and corrosivity problems. The means of removing hydrogen sulphide mainly depend on its concentration and kind of medium to be purified. The paper deals with a method of hydrogen sulphide removal from the air by its catalytic oxidation to elemental sulphur with the use of Fe-EDTA complex. The possibility of obtaining fibrous filtering materials able to remove small concentrations of H2S from the air were described. The base of these materials is fibrous ion exchanger with Fe(III)- EDTA complex immobilized on their functional groups. The complex of trivalent iron converts hydrogen sulphide to elemental sulphur. Bivalent iron formed in the reaction is oxidized by the atmospheric oxygen, so complex of trivalent iron is continuously regenerated and the overall process can be accounted as pseudocatalytic. In the present paper properties of several fibrous catalysts based on ion exchangers with different chemical nature (weak acid,weak base and strong base) were described. It was shown that the main parameters affecting the process of catalytic oxidation are:concentration of hydrogen sulphide in the air, relative humidity of the purified air, the process time and the content of Fe-EDTA complex in the fibres. The data presented show that the filtering layers with anion exchange package are much more active in the catalytic processes of hydrogen sulphide removal than cation exchanger and inert materials. In the addition to the nature of the fibres relative air humidity is a critical factor determining efficiency of the material in the air purification from H2S. It was proved that the most promising carrier of the Fe-EDTA catalyst for hydrogen sulphide oxidation are Fiban A-6 and Fiban AK-22 fibres.
Abstract: Mobile WiMAX is a broadband wireless solution that
enables convergence of mobile and fixed broadband networks
through a common wide area broadband radio access technology and
flexible network architecture. It adopts Orthogonal Frequency
Division Multiple Access (OFDMA) for improved multi-path
performance in Non-Line-Of-Sight (NLOS) environments. Scalable
OFDMA (SOFDMA) is introduced in the IEEE 802e[1]. WIMAX
system uses one of different types of channel coding but The
mandatory channel coding scheme is based on binary nonrecursive
Convolutional Coding (CC). There are other several optional channel
coding schemes such as block turbo codes, convolutional turbo
codes, and low density parity check (LDPC).
In this paper a comparison between the performance of WIMAX
using turbo code and using convolutional product code (CPC) [2] is
made. Also a combination between them had been done. The CPC
gives good results at different SNR values compared to both the
turbo system, and the combination between them. For example, at
BER equal to 10-2 for 128 subcarriers, the amount of improvement
in SNR equals approximately 3 dB higher than turbo code and equals
approximately 2dB higher than the combination respectively. Several
results are obtained at different modulating schemes (16QAM and
64QAM) and different numbers of sub-carriers (128 and 512).
Abstract: In this paper we study the resource allocation problem
for an OFDMA based cooperative two-way relaying (TWR) network.
We focus on amplify and forward (AF) analog network coding
(ANC) protocol. An optimization problem for two basic resources
namely, sub-carrier and power is formulated for multi-user TWR
networks. A joint optimal optimization problem is investigated and
two-step low complexity sub-optimal resource allocation algorithm is
proposed for multi-user TWR networks with ANC protocol. The
proposed algorithm has been evaluated in term of total achievable
system sum-rate and achievable individual sum-rate for each userpair.
The good tradeoff between system sum-rate and fairness is
observed in the two-step proportional resource allocation scheme.
Abstract: Computer technology and the Internet have made a
breakthrough in the existence of data communication. This has
opened a whole new way of implementing steganography to ensure
secure data transfer. Steganography is the fine art of hiding the
information. Hiding the message in the carrier file enables the
deniability of the existence of any message at all. This paper designs
a stego machine to develop a steganographic application to hide data
containing text in a computer video file and to retrieve the hidden
information. This can be designed by embedding text file in a video
file in such away that the video does not loose its functionality using
Least Significant Bit (LSB) modification method. This method
applies imperceptible modifications. This proposed method strives
for high security to an eavesdropper-s inability to detect hidden
information.
Abstract: The purpose of the experiments described in this article was the comparison of integrated fixed film activated sludge (IFAS) and activated sludge (AS) system. The IFAS applied system consists of the cigarette filter rods (wasted filter in tobacco factories) as a biofilm carrier. The comparison with activated sludge was performed by two parallel treatment lines. Organic substance, ammonia and TP removal was investigated over four month period. Synthetic wastewater was prepared with ordinary tap water and glucose as the main sources of carbon and energy, plus balanced macro and micro nutrients. COD removal percentages of 94.55%, and 81.62% were achieved for IFAS and activated sludge system, respectively. Also, ammonia concentration significantly decreased by increasing the HRT in both systems. The average ammonia removal of 97.40 % and 96.34% were achieved for IFAS and activated sludge system, respectively. The removal efficiency of total phosphorus (TP-P) was 60.64%, higher than AS process by 56.63% respectively.
Abstract: Transcription factor p53 has a powerful tumor
suppressing function that is associated with many cancers. However,
p53 of the molecular weight was higher make the limitation across to
skin or cell membrane. Thymidine dinucleotide (pTT), an
oligonucleotide, can activate the p53 transcription factor. pTT is a
hydrophilic and negative charge oligonucleotide, which delivery in to
cell membrane need an appropriate carrier. The aim of this study was
to improve the bioavailability of the nucleotide fragment, thymidine
dinucleotide (pTT), using elasic liposome carriers to deliver the drug
into the skin. The study demonstrate that dioleoylphosphocholine
(DOPC) incorporated with sodium cholate at molar ratio 1:1 can
archived the particle size about 220 nm. This elastic liposome could
penetration through skin from stratum corneum to whole epidermis by
confocal laser scanning microscopy (CLSM). Moreover, we observed
the the slight increase in generation of p53 by western blot.
Abstract: Topical photodynamic therapy (PDT) with
5-aminolevulinic acid (ALA) is an alternative therapy for treating
superficial cancer, especially for skin or oral cancer. ALA, a precursor
of the photosensitizer protoporphyrin IX (PpIX), is present as
zwitterions and hydrophilic property which make the low permeability
through the cell membrane. Collagen is a traditional carrier; its
molecular composed various amino acids which bear positive charge
and negative charge. In order to utilize the ion-pairs with ALA and
collagen, the study employed various pH values adjusting the net
charge. The aim of this study was to compare a series collagen form,
including solution, gel and sponge to investigate the topical delivery
behavior of ALA. The in vivo confocal laser scanning microscopy
(CLSM) study demonstrated that PpIX generation ability was different
pattern after apply for 6 h. Gel type could generate high PpIX, and
archived more deep of skin depth.
Abstract: RF performance of SOI CMOS device has attracted
significant amount of interest recently. In order to improve RF
parameters, Strained Si/Relaxed Si0.8Ge0.2 investigated as a
replacement for Si technology .Enhancement of carrier mobility
associated with strain engineering makes Strained Si a promising
candidate for improving RF performance of CMOS technology.
From the simulation, the cut-off frequency is estimated to be 224
GHZ, whereas in SOI at similar bias is about 188 GHZ. Therefore,
Strained Si exhibits 19% improvement in cut-off frequency over
similar Si counterpart. In this paper, Ion/Ioff ratio is studied as one of
the key parameters in logic and digital application. Strained Si/SiGe
demonstrates better Ion/Ioff characteristic than SOI, in similar channel
length of 100 nm.Another important key analog figures of merit such
as Early Voltage (VEA) ,transconductance vs drain current (gm /Ids)
are studied. They introduce the efficiency of the devices to convert
dc power into ac frequency.
Abstract: Identifying the nature of protein-nanoparticle
interactions and favored binding sites is an important issue in
functional characterization of biomolecules and their physiological
responses. Herein, interaction of silver nanoparticles with lysozyme
as a model protein has been monitored via fluorescence spectroscopy.
Formation of complex between the biomolecule and silver
nanoparticles (AgNPs) induced a steady state reduction in the
fluorescence intensity of protein at different concentrations of
nanoparticles. Tryptophan fluorescence quenching spectra suggested
that silver nanoparticles act as a foreign quencher, approaching the
protein via this residue. Analysis of the Stern-Volmer plot showed
quenching constant of 3.73 μM−1. Moreover, a single binding site in
lysozyme is suggested to play role during interaction with AgNPs,
having low affinity of binding compared to gold nanoparticles.
Unfolding studies of lysozyme showed that complex of lysozyme-
AgNPs has not undergone structural perturbations compared to the
bare protein. Results of this effort will pave the way for utilization of
sensitive spectroscopic techniques for rational design of
nanobiomaterials in biomedical applications.
Abstract: A novel thermo-sensitive superabsorbent hydrogel
with salt- and pH-responsiveness properties was obtained by grafting
of mixtures of acrylic acid (AA) and N-isopropylacrylamide
(NIPAM) monomers onto kappa-carrageenan, kC, using ammonium
persulfate (APS) as a free radical initiator in the presence of
methylene bisacrylamide (MBA) as a crosslinker. Infrared
spectroscopy was carried out to confirm the chemical structure of the
hydrogel. Moreover, morphology of the samples was examined by
scanning electron microscopy (SEM). The effect of MBA
concentration and AA/NIPAM weight ratio on the water absorbency
capacity has been investigated. The swelling variations of hydrogels
were explained according to swelling theory based on the hydrogel
chemical structure. The hydrogels exhibited salt-sensitivity and
cation exchange properties. The temperature- and pH-reversibility
properties of the hydrogels make the intelligent polymers as good
candidates for considering as potential carriers for bioactive agents,
e.g. drugs.
Abstract: This paper present some preliminary work on the
preparation and physicochemical caracterization of nanocomposite
MFI-alumina structures based on alumina hollow fibres. The fibers
are manufactured by a wet spinning process. α-alumina particles were
dispersed in a solution of polysulfone in NMP. The resulting slurry is
pressed through the annular gap of a spinneret into a precipitation
bath. The resulting green fibres are sintered. The mechanical strength
of the alumina hollow fibres is determined by a three-point-bending
test while the pore size is characterized by bubble-point testing. The
bending strength is in the range of 110 MPa while the average pore
size is 450 nm for an internal diameter of 1 mm and external diameter
of 1.7 mm. To characterize the MFI membranes various techniques
were used for physicochemical characterization of MFI–ceramic
hollow fibres membranes: The nitrogen adsorption, X-ray
diffractometry, scanning electron microscopy combined with X
emission microanalysis. Scanning Electron Microscopy (SEM) and
Energy Dispersive Microanalysis by the X-ray were used to observe
the morphology of the hollow fibre membranes (thickness,
infiltration into the carrier, defects, homogeneity). No surface film,
has been obtained, as observed by SEM and EDX analysis and
confirmed by high temperature variation of N2 and CO2 gas
permeances before cation exchange. Local analysis and characterise
(SEM and EDX) and overall (by ICP elemental analysis) were
conducted on two samples exchanged to determine the quantity and
distribution of the cation of cesium on the cross section fibre of the
zeolite between the cavities.
Abstract: In the current study we present a system that is
capable to deliver proxy based differentiated service. It will help the
carrier service node to sell a prepaid service to clients and limit the
use to a particular mobile device or devices for a certain time. The
system includes software and hardware architecture for a mobile
device with moderate computational power, and a secure protocol for
communication between it and its carrier service node. On the
carrier service node a proxy runs on a centralized server to be
capable of implementing cryptographic algorithms, while the mobile
device contains a simple embedded processor capable of executing
simple algorithms. One prerequisite is needed for the system to run
efficiently that is a presence of Global Trusted Verification Authority
(GTVA) which is equivalent to certifying authority in IP networks.
This system appears to be of great interest for many commercial
transactions, business to business electronic and mobile commerce,
and military applications.
Abstract: Multi-level voltage source inverters offer several
advantages such as; derivation of a refined output voltage with
reduced total harmonic distortion (THD), reduction of voltage ratings
of the power semiconductor switching devices and also the reduced
electro-magnetic-interference problems etc. In this paper, new
carrier-overlapped phase-disposition or sub-harmonic sinusoidal
pulse width modulation (CO-PD-SPWM) and also the carrieroverlapped
phase-disposition space vector modulation (CO-PDSVPWM)
schemes for a six-level diode-clamped inverter topology
are proposed. The principle of the proposed PWM schemes is similar
to the conventional PD-PWM with a little deviation from it in the
sense that the triangular carriers are all overlapped. The overlapping
of the triangular carriers on one hand results in an increased number
of switchings, on the other hand this facilitates an improved spectral
performance of the output voltage. It is demonstrated through
simulation studies that the six-level diode-clamped inverter with the
use of CO-PD-SPWM and CO-PD-SVPWM proposed in this paper is
capable of generating multiple levels in its output voltage. The
advantages of the proposed PWM schemes can be derived to benefit,
especially at lower modulation indices of the inverter and hence this
aspect of the proposed PWM schemes can be well exploited in high
power applications requiring low speeds of operation of the drive.
Abstract: Microparticles carrier systems made from naturally occurring polymers based on chitosan/casein system appears to be a promising carrier for the sustained release of orally and parenteral administered drugs. In the current study we followed a microencapsulation technique based aqueous coacervation method to prepare chitosan/casein microparticles of compositions 1:1, 1:2 and 1:5 incorporated with chloramphenicol. Glutaraldehyde was used as a chemical cross-linking agent. The microparticles were prepared by aerosol method and studied by optical microscopy, infrared spectroscopy, thermo gravimetric analysis, swelling studies and drug release studies at various pH. The percentage swelling of the polymers are found to be in the order pH 4 > pH 10 > pH 7 and the increase in casein composition decrease the swelling percentage. The drug release studies also follow the above order.
Abstract: Vermiculite was used to develop inorganic
carrier-based formulations of fluorescent pseudomonad strains
R62 and R81. The effect of bio-inoculation of fluorescent
pseudomonad strains R62 and R81 (plant growth promoting
and biocontrol agent) on growth responses of Vigna-mungo
under field condition was enumerated. The combined bioinoculation
of these two organisms in a formuation increased
the pods yield by 300% in comparison to the control crop.
There was also significant increment in the other plant growth
responses such as dry root weight, dry shoot weight, shoot
length and number of branches per plant.
Abstract: The paper relates to a catalyst, comprising copperchromium
spinel, coated on carrier γ-Al2O3. The effect of preparation
conditions on the active component composition and activity
behavior of the catalysts is discussed. It was found that the activity of
carbon monoxide, DME, formaldehyde and methanol oxidation
reaches a maximum at an active component content of 20 – 30 wt. %.
Temperature calcination at 500oC seems to be optimal for the γ–
alumina supported CuO-Cr2O3 catalysts for CO, DME, formaldehyde
and methanol oxidation. A three months industrial experiment was
carried out to elucidate the changes in the catalyst composition
during industrial exploitation of the catalyst and the main reasons for
catalyst deactivation.
It was concluded that the CuO–Cr2O3/γ–alumina supported
catalysts have enhanced activity toward CO, DME, formaldehyde
and methanol oxidation and that these catalysts are suitable for
industrial application. The main reason for catalyst deactivation
seems to be the deposition of iron and molybdenum, coming from the
main reactor, on the active component surface.