Aim of this work was to compare the efficacy of two
loading methods of proteins onto polymeric nanocarriers: adsorption
and encapsulation methods. Preliminary studies of protein loading
were done using Bovine Serum Albumin (BSA) as model protein.
Nanocarriers were prepared starting from polylactic co-glycolic acid
(PLGA) polymer; production methods used are two different variants
of emulsion evaporation method. Nanoparticles obtained were
analyzed in terms of dimensions by Dynamic Light Scattering and
Loading Efficiency of BSA by Bradford Assay. Loaded
nanoparticles were then submitted to in-vitro protein dissolution test
in order to study the effect of the delivery system on the release rate
of the protein.
[1] M. Singh, R. Sharma and U.C. Banerjee, "Biotechnological applications
of cyclodextrins" Biotechnol Adv, vol. 20; no 5-6, pp.341-359, 2002.
[2] P. Couvreur and C. Vauthier, " Nanotechnology: Intelligent design to
treat complex disease", Pharm Res, vol 7, no, 23, pp 1417-1450, July 2006
[3] M.F. Francis, M. Cristea, F.M. Winnik, "Polymeric micelles for oral
drug delivery: Why and how", Pure Appl Chem, vol.76 , no.7-8, pp1321-
1335, 2004;.
[4] R.C. Mundargi, V.R. Babu, V. Rangaswamy, P. Patel and T.M.
Aminabhavi "Nano/micro technologies for delivering macromolecular
therapeutics using poly(D,L-lactide-co-glycolide) and its derivatives" J
Control Rel, Vol.125,no.3, pp 193-209, 2008.
[5] C. Pinto-Reis, R.J. Neufeld, A.J. Ribeiro and F. Veiga
"Nanoencapsulation II. Biomedical applications and current status of peptide
and protein nanoparticulate delivery systems", Nanomedicine, vol.2,no.2 pp
53- 65; June 2006.
[6] K. Tahara, T. Sakai, H. Yamamoto, H. Takeuchi and Y. Kawashima,
"Establishing chitosan coated PLGA nanosphere platform loaded with wide
variety of nucleic acid by complexation with cationic compound for gene
delivery", Int J Pharm, no. 354, pp 210-216, February 2008;.
[7] D. Lamalle-Bernard, S. Munier, C. Compagnon, M.H. Charles, V.S.
Kalyanaraman, T. Delair, B. Verrier and Y. Ataman-Őnal, "Coadsorption of
HIV-1 p24 and gp120 proteins to surfactant-free anionic PLA nanoparticles
preserves antigenicity and immunogenicity", J Control Rel, no. 115, pp. 57-
67, July 2006.
[8] C. Cai, U. Bakowsky, E. Rytting, A.K. Schaper and T. Kissel, "Charged
nanoparticles as protein delivery systems: A feasibility study using lysozyme
as model protein", Eur J Pharm Biopharm, no. 69, pp. 31-42, 2008.
[9] M.A. Dobrovolskaia and S.E. McNeil, "Immunological properties of
engineered nanomaterials", Nat Nanotechnol, vol 2, pp. 469-478, August
2007;
[10] P Gong and I Szleifer, "Competitive adsorption of model charged
proteins: the effect of total charge and charge distribution", J Colloid Interface
Sci, no. 278, pp. 81-90, June 2004.
[11] J.H. Kim, A. Taluja, K. Knutson, Y.H. Bae, "Stability of bovine
serum albumin complexed with PEG-poly(L-histidine) diblock copolymer in
PLGA microspheres", J Control Rel, no. 109, pp. 86-100, November 2005.
[12] I. Lynch, T. Cedervall, M. Lundqvist, C. Cabaleiro-Lago, S. Linse
and K.A. Dawson, "The nanoparticle-protein complex as a biological entity;
a complex fluids and surface science challenge for the 21st century", Adv
Colloid and Interface Sci, Vol 134-135, pp. 167-174, October 2007.
[13] E.D. Kaufman, J. Belyea, M.C. Johnson, Z.M. Nicholson, J.L. Ricks,
P.K. Shah, M. Bayless, T. Pettersson, Z. Feldoto, E. Blomberg, P. Claesson
and S. Franzen, "Probing protein adsorption onto mercaptoundecanoic acid
stabilized gold nanoparticles and surfaces by quartz crystal microbalance and
╬Â-potential measurements", Langmuir, no. 23, pp.6053-6062, March 2007.
[1] M. Singh, R. Sharma and U.C. Banerjee, "Biotechnological applications
of cyclodextrins" Biotechnol Adv, vol. 20; no 5-6, pp.341-359, 2002.
[2] P. Couvreur and C. Vauthier, " Nanotechnology: Intelligent design to
treat complex disease", Pharm Res, vol 7, no, 23, pp 1417-1450, July 2006
[3] M.F. Francis, M. Cristea, F.M. Winnik, "Polymeric micelles for oral
drug delivery: Why and how", Pure Appl Chem, vol.76 , no.7-8, pp1321-
1335, 2004;.
[4] R.C. Mundargi, V.R. Babu, V. Rangaswamy, P. Patel and T.M.
Aminabhavi "Nano/micro technologies for delivering macromolecular
therapeutics using poly(D,L-lactide-co-glycolide) and its derivatives" J
Control Rel, Vol.125,no.3, pp 193-209, 2008.
[5] C. Pinto-Reis, R.J. Neufeld, A.J. Ribeiro and F. Veiga
"Nanoencapsulation II. Biomedical applications and current status of peptide
and protein nanoparticulate delivery systems", Nanomedicine, vol.2,no.2 pp
53- 65; June 2006.
[6] K. Tahara, T. Sakai, H. Yamamoto, H. Takeuchi and Y. Kawashima,
"Establishing chitosan coated PLGA nanosphere platform loaded with wide
variety of nucleic acid by complexation with cationic compound for gene
delivery", Int J Pharm, no. 354, pp 210-216, February 2008;.
[7] D. Lamalle-Bernard, S. Munier, C. Compagnon, M.H. Charles, V.S.
Kalyanaraman, T. Delair, B. Verrier and Y. Ataman-Őnal, "Coadsorption of
HIV-1 p24 and gp120 proteins to surfactant-free anionic PLA nanoparticles
preserves antigenicity and immunogenicity", J Control Rel, no. 115, pp. 57-
67, July 2006.
[8] C. Cai, U. Bakowsky, E. Rytting, A.K. Schaper and T. Kissel, "Charged
nanoparticles as protein delivery systems: A feasibility study using lysozyme
as model protein", Eur J Pharm Biopharm, no. 69, pp. 31-42, 2008.
[9] M.A. Dobrovolskaia and S.E. McNeil, "Immunological properties of
engineered nanomaterials", Nat Nanotechnol, vol 2, pp. 469-478, August
2007;
[10] P Gong and I Szleifer, "Competitive adsorption of model charged
proteins: the effect of total charge and charge distribution", J Colloid Interface
Sci, no. 278, pp. 81-90, June 2004.
[11] J.H. Kim, A. Taluja, K. Knutson, Y.H. Bae, "Stability of bovine
serum albumin complexed with PEG-poly(L-histidine) diblock copolymer in
PLGA microspheres", J Control Rel, no. 109, pp. 86-100, November 2005.
[12] I. Lynch, T. Cedervall, M. Lundqvist, C. Cabaleiro-Lago, S. Linse
and K.A. Dawson, "The nanoparticle-protein complex as a biological entity;
a complex fluids and surface science challenge for the 21st century", Adv
Colloid and Interface Sci, Vol 134-135, pp. 167-174, October 2007.
[13] E.D. Kaufman, J. Belyea, M.C. Johnson, Z.M. Nicholson, J.L. Ricks,
P.K. Shah, M. Bayless, T. Pettersson, Z. Feldoto, E. Blomberg, P. Claesson
and S. Franzen, "Probing protein adsorption onto mercaptoundecanoic acid
stabilized gold nanoparticles and surfaces by quartz crystal microbalance and
╬Â-potential measurements", Langmuir, no. 23, pp.6053-6062, March 2007.
@article{"International Journal of Medical, Medicine and Health Sciences:58006", author = "G. Spada and E. Gavini and P. Giunchedi", title = "Protein Delivery from Polymeric Nanoparticles", abstract = "Aim of this work was to compare the efficacy of two
loading methods of proteins onto polymeric nanocarriers: adsorption
and encapsulation methods. Preliminary studies of protein loading
were done using Bovine Serum Albumin (BSA) as model protein.
Nanocarriers were prepared starting from polylactic co-glycolic acid
(PLGA) polymer; production methods used are two different variants
of emulsion evaporation method. Nanoparticles obtained were
analyzed in terms of dimensions by Dynamic Light Scattering and
Loading Efficiency of BSA by Bradford Assay. Loaded
nanoparticles were then submitted to in-vitro protein dissolution test
in order to study the effect of the delivery system on the release rate
of the protein.", keywords = "Drug delivery, nanoparticles, PLGA, proteinadsorption, protein encapsulation.", volume = "5", number = "4", pages = "146-5", }
