Abstract: The development of Drugs Delivery System (DDS)
has been widely investigated in the last decades. In this paper, first a
general overview of traditional and modern wound dressing is
presented. This is followed by a review of what scientists have done
in the medical environment, focusing on the possibility to develop a
new alternative for DDS through transdermal pathway, aiming to
treat melanoma skin cancer.
Abstract: In this paper, a pipelined version of genetic algorithm,
called PLGA, and a corresponding hardware platform are described.
The basic operations of conventional GA (CGA) are made pipelined
using an appropriate selection scheme. The selection operator, used
here, is stochastic in nature and is called SA-selection. This helps
maintaining the basic generational nature of the proposed pipelined
GA (PLGA). A number of benchmark problems are used to compare
the performances of conventional roulette-wheel selection and the
SA-selection. These include unimodal and multimodal functions with
dimensionality varying from very small to very large. It is seen that
the SA-selection scheme is giving comparable performances with
respect to the classical roulette-wheel selection scheme, for all the
instances, when quality of solutions and rate of convergence are considered.
The speedups obtained by PLGA for different benchmarks
are found to be significant. It is shown that a complete hardware
pipeline can be developed using the proposed scheme, if parallel
evaluation of the fitness expression is possible. In this connection
a low-cost but very fast hardware evaluation unit is described.
Results of simulation experiments show that in a pipelined hardware
environment, PLGA will be much faster than CGA. In terms of
efficiency, PLGA is found to outperform parallel GA (PGA) also.
Abstract: Aim of this work was to compare the efficacy of two
loading methods of proteins onto polymeric nanocarriers: adsorption
and encapsulation methods. Preliminary studies of protein loading
were done using Bovine Serum Albumin (BSA) as model protein.
Nanocarriers were prepared starting from polylactic co-glycolic acid
(PLGA) polymer; production methods used are two different variants
of emulsion evaporation method. Nanoparticles obtained were
analyzed in terms of dimensions by Dynamic Light Scattering and
Loading Efficiency of BSA by Bradford Assay. Loaded
nanoparticles were then submitted to in-vitro protein dissolution test
in order to study the effect of the delivery system on the release rate
of the protein.
Abstract: The expression of LFA-1 diverges from the
physiological condition, thus active targeting carrier can provide the
benefits from difference into LFA-1 expression in various conditions.
Here, the selectivity of cIBR-conjugated nanoparticles (cIBR-NPs),
in terms of uptake, was investigated using PBMCs, Mixed PBMCMolt-
3 cells and Molt-3 cells. The expressions of LFA-1 on Molt-3
cells, from flow cytometry and Western blot, possessed the highest
level whereas PBMCs showed the lowest level. The kinetic uptake
profiles of cIBR-NPs were obtained by flow cytometry, which the
degree of cellular uptake presented a similar trend with the level of
LFA-1 indicating the influence of LFA-1 expression on the cellular
uptake of cIBR-NPs. The conformation of LFA-1 had a slight effect
on the cellular uptake of cIBR-NPs. Overall we demonstrated that
cIBR-NPs enhanced cellular uptake and improved the selectivity of
drug carriers to LFA-1 on the leukemia cells, which related with the
order of LFA-1 expression.