Gastroprotective Activity of Swietenia Mahagoni Seed Extract on Ethanol-Induced Gastric Mucosal Injury in Rats
Swietenia mahagoni have been used in traditional
medicine for treatment of different diseases. Present study was
performed to evaluate anti-ulcerogenic activity of ethanol seed
extract against ethanol induced gastric mucosal injury in rats. Six
groups of rats were orally pre-treated respectively with
carboxymethyl cellulose, omeprazole 20 mg/kg, 50, 100, 200 and 400
mg/kg plant extract one hour before oral administration of absolute
ethanol to generate gastric mucosal injury. After additional hour, rats
were sacrificed and ulcer areas of gastric walls were determined.
Grossly, carboxymethyl cellulose group exhibited severe mucosal
injury, whereas pre-treatment with plant extract exhibited significant
protection of gastric mucosa. Histology, carboxymethyl cellulose
group exhibited severe damage of gastric mucosa; edema and
leucocytes infiltration of sub mucosa compared to plant extract which
showed gastric protection. Acute toxicity study did not manifest any
toxicological signs in rats. Conclusions, results suggest that S.
mahagoni promotes ulcer protection as ascertained grossly and
histologically.
[1] Mulholland, D., B. Parel, and P. Coombes. The chemistry of the
Meliaceae and Ptaeroxylaceae of southern and eastern Africa and
Madagascar. Current Organic Chemistry, 2000, 4(10): p. 1011-1054.
[2] Munoz, V., Sauvain, M., Bourdy, G., Callapa, J., Rojas, I., Vargas, L.,
Tae A., and Deharo, E. The search for natural bioactive compounds
through a multidisciplinary approach in Bolivia. Part II. Antimalarial
activity of some plants used by Mosetene Indians. Journal of
ethnopharmacology, 2000, 69(2): 139-155.
[3] Maiti, A., S. Dewanjee, and S. Mandal. In vivo evaluation of
antidiarrhoeal activity of the seed of Swietenia macrophylla King
(Meliaceae). Tropical Journal of Pharmaceutical Research, 2007, 6(2):
711-716.
[4] Majid, M., Rahman, I.M.M.; Shipar, M.A.H.; Uddin, M.H.;
Chowdhury, R.. Physico-chemical characterization, antimicrobial
activity and toxicity analysis of Swietenia mahagoni seed oil.
International Journal of Agriculture and Biology (Pakistan), 2004,
6(2),350-354.
[5] Govindachari, T.; Suresh G.; Banumathy B.; Masilamani S.;
Gopalakrishnan G.; KrishnaKumari G.N. Antifungal activity of some
B, D-seco limonoids from two meliaceous plants. Journal of Chemical
Ecology,1999, 25(4): 923-933.
[6] Li, D., Chen, J.H., Chen, Q., Li., G.W, Chen, J., Yue, J.M., Chen, M.L.,
Wang, X.P., Shen, J.H., Shen, X., Jiang, H.L. Swietenia mahagony
extract shows agonistic activity to PPAR and gives ameliorative effects
on diabetic db/db mice. Acta Pharmacologica Sinica, 2005, 26(2): 220-
222.
[7] Nagalakshmi, M.; Thangadurai, D.; Muralidara, D.; Pullaiah, R.T.
Phytochemical and antimicrobial study of Chukrasia tabularis leaves.
Fitoterapia, 2001, 72(1): 62-64.
[8] Bacsal, K.; Havez, L.; Diaz, I.; Espina, S.; Javillo, J.; Manzanilla, H.;
Motalban, J.; Panganiban, C.; Ro driguez, A.; Sumpaico, C.; Talip, B.;
Yap, S. The effect of Switenia mahogany (mahogany) seed extract on
indomethacin-induced gastric ulcers in female Sprague-Dawley rats.
Acta Medica Philippina, 1997, 3:127-139.
[9] Sahgal, G., Ramanathan S, Sasidharan S, Mordi MN, Ismail S, Mansor
SM. In Vitro Antioxidant and Xanthine Oxidase Inhibitory Activities of
Methanolic Swietenia mahagoni Seed Extracts. Molecules (Basel,
Switzerland), 2009, 14(11): 4476-85.
[10] Sahgal, G., Ramanathan, S., Sasidharan, S., Mordi, M.N., Ismail, S. and
Mansor, S.M. Phytochemical and antimicrobial activity of Swietenia
mahagoni crude methanolic seed extract. Tropical Biomedicine,2009,
26(3): 274-279.
[11] Li, X., Andersson, T.B., Ahlstom, M., Weidolf, L. Comparison of
inhibitory effects of the proton pump-inhibiting drugs omeprazole,
esomeprazole, lansoprazole, pantoprazole, and rabeprazole on human
cytochrome P450 activities. Drug metabolism and disposition, 2004,
32(8): 821-827.
[12] Satoh, H., Inatomi, N., Nagaya, H., Ianda, I., Nohara, A., Nakamura, H.
Antisecretory and antiulcer activities of a novel proton pump inhibitor
AG-1749 in dogs and rats. Journal of Pharmacology and Experimental
Therapeutics, 1989, 248(2): 806-815.
[13] Nagaya, H., Inatomi, N., Ohara, A., Satoh, H. (1991). Effects of the
enantiomers of lansoprazole (AG-1749) on (H++ K+)-ATPase activity
in canine gastric microsomes and acid formation in isolated canine
parietal cells. Biochemical pharmacology, 42(10): 1875-1878.
[14] Pedernera, A.M., Guardia, T., Caleron, C.G., Rotelli, A.F., de la Rocha,
N.F., di Genaro, S., Pelze, L.F. Anti-ulcerogenic and anti-inflammatory
activity of the methanolic extract of Larrea divaricata Cav. in rat.
Journal of ethnopharmacology, 2006, 105(3): 415-420.
[15] De Pasquale, R., Germano, M.P., Keita, A., Sanogo, R., Lauk, L.
Antiulcer activity of Pteleopsis suberosa. Journal of
ethnopharmacology, 1995, 47(1): 55-58.
[16] Bergmeyer, H. and M. Horder. IFCC methods for the measurement of
catalytic concentration of enzymes, Part 3. IFCC method for alanine
aminotransferase. J Clin Chem Clin Biochem, 1980, 18: 521-534.
[17] Tietz, N.W., Rinker, A.D., Shaw, L.M. (1983). IFCC methods for the
measurement of catalytic concentration of enzymes, Part 5. IFCC
method for alkaline phosphatase. J Clin Chem Clin Biochem, 1983,
21(11): 731-748.
[18] Garg, G.P., Nigam, S.K., Ogle, C.W. The gastric antiulcer effects of the
leaves of the neem tree. Planta medica, 1993, 59: 215-215.
[19] Hollander, D., Taranawski, A., Krause, W.J., Gergely, H. Protective
effect of sucralfate against alcohol-induced gastric mucosal injury in
the rat. Macroscopic, histologic, ultrastructural, and functional time
sequence analysis. Gastroenterology, 1985, 88(1 Pt 2): 366-374.
[20] Paiva, L.A.F, Rao, V.S.N., Gramosa, N.V., Silveira, F.R.
Gastroprotective effect of Copaifera langsdorffii oleo-resin on
experimental gastric ulcer models in rats. Journal of
ethnopharmacology, 1998, 62(1): 73-78.
[21] Kauffman, G. L., Grossman, M. I. Prostaglandin and cimetidine inhibit
the formation of ulcers produced by parenteral salicylates.
Gastroenterology, 1978, 75(6): 1099-2102.
[22] Njar V. C. O., Adesanwo J. K., Raji Y. Methyl Angolenate: The
Antiulcer Agent from the Stem Bark of Entandrophragma angolense.
Planta medica, 1995, 61: 91-92.
[23] Piper, D.W. and Stiel, D. (1986). Pathogenesis of chronic peptic ulcer,
current thinking and clinical implications. Med Prog, 2: 7-10.
[24] Baron, T.H., Langman, M.T.S. and Wastell, C. Stomach and
duodenum. Recent advances in gastroenterology. Churchill
Livingstone, Edinburgh, 1980, p. 23-86.
[25] Szabo, S., Kusstatscher, S, Sakoulas, G, Sandor, Z, Vincze, A & Jadus,
M. Growth factors: new ÔÇÿendogenous drugs- for ulcer healing.
Scandinavian Journal of Gastroenterology, 1995, 30: p. 15-18.
[26] Marhuenda, E., Martin, M.J. and Alarcon de la Lastra, C.
Antiulcerogenic activity of aescine in different experimental models.
Phytotherapy Research, 2006, 7(1): 13-16.
[27] Mutoh, H., Hiraishi, H., Ota, S., Ivey, K.J. Terano, A. Sugimoto, T.
Role of oxygen radicals in ethanol-induced damage to cultured gastric
mucosal cells. American Journal of Physiology- Gastrointestinal and
Liver Physiology, 1990, 258(4): G603-G609.
[28] Trivedi, N.P., Rawal, U.M. Hepatoprotective and antioxidant property
of Andrographis paniculata (Nees) in BHC induced liver damage in
mice. Indian Journal of Experimental Biology, 2001, 39(1): 41-46.
[29] Ligumsky, M., Sestieri, M., Okon, F., Ginsburg, I. Antioxidants inhibit
ethanol-induced gastric injury in the rat: role of manganese, glycine,
and carotene. Scandinavian Journal of Gastroenterology, 1995, 30(9):
854-860.
[30] Kobayashi, T., Ohta, Y., Yoshino, J., Nakazawa, S. Teprenone
promotes the healing of acetic acid-induced chronic gastric ulcers in
rats by inhibiting neutrophil infiltration and lipid peroxidation in
ulcerated gastric tissues. Pharmacological Research, 2001, 43(1): 23-
30.
[31] Shimizu, N., Watanabe T, Arakawa T, Fujiwara Y, Higuchi K, Kuroki
T. Pentoxifylline accelerates gastric ulcer healing in rats: roles of tumor
necrosis factor alpha and neutrophils during the early phase of ulcer
healing. Digestion, 2000, 61(3): 157-164.
[32] Cheng CL, Koo MWL. Effects of Centella asiatica on ethanol induced
gastric mucosal lesions in rats. Life sciences, 2000, 67(21): 2647-2653.
[33] Fujita, H, Takahashi, S., Okabe, S. Mechanism by which indomethacin
delays the healing of acetic acid-induced ulcers in rats. Role of
neutrophil antichemotactic and chemotactic activities. Journal of
physiology and pharmacology: an official journal of the Polish
Physiological Society, 1998, 49(1): 71-82.
[34] Suzuki, Y., Ishihara M, Ito M. Anti-ulcer effects of antioxidants,
quercetin, -tocopherol, nifedipine and tetracycline in rats. The Japanese
Journal of Pharmacology, 1998, 78(4): p. 435-441.
[35] Zimmerman, J.J. Ciesielski, W., Lewandoski, J. Neutrophil-mediated
phospholipid peroxidation assessed by gas chromatography-mass
spectroscopy. American Journal of Physiology- Cell Physiology, 1997,
273(2): C653-661.
[36] Garrick, T., Buack, S., Bass, P. Gastric motility is a major factor in
cold restraint-induced lesion formation in rats. American Journal of
Physiology- Gastrointestinal and Liver Physiology, 1986, 250(2):191-
199.
[37] Takeuchi, K., Nishiwakki, K., Okabe, S. Effects of dopamine on gastric
mucosal lesions induced by ethanol in rats. Digestive Diseases and
Sciences, 1988, 33(12): 1560-1568.
[38] Takeuch, K. and Nobuhara, Y. Inhibition of gastric motor activity by
16, 16-dimethyl prostaglandin E 2. Digestive Diseases and Sciences,
1985, 30(12): 1181-1188.
[39] Mersereau, A.W. and Hinchey, E.J. Role of gastric mucosal folds in
formation of focal ulcers in the rat. Surgery, 1982, 91(2):150-155.
[1] Mulholland, D., B. Parel, and P. Coombes. The chemistry of the
Meliaceae and Ptaeroxylaceae of southern and eastern Africa and
Madagascar. Current Organic Chemistry, 2000, 4(10): p. 1011-1054.
[2] Munoz, V., Sauvain, M., Bourdy, G., Callapa, J., Rojas, I., Vargas, L.,
Tae A., and Deharo, E. The search for natural bioactive compounds
through a multidisciplinary approach in Bolivia. Part II. Antimalarial
activity of some plants used by Mosetene Indians. Journal of
ethnopharmacology, 2000, 69(2): 139-155.
[3] Maiti, A., S. Dewanjee, and S. Mandal. In vivo evaluation of
antidiarrhoeal activity of the seed of Swietenia macrophylla King
(Meliaceae). Tropical Journal of Pharmaceutical Research, 2007, 6(2):
711-716.
[4] Majid, M., Rahman, I.M.M.; Shipar, M.A.H.; Uddin, M.H.;
Chowdhury, R.. Physico-chemical characterization, antimicrobial
activity and toxicity analysis of Swietenia mahagoni seed oil.
International Journal of Agriculture and Biology (Pakistan), 2004,
6(2),350-354.
[5] Govindachari, T.; Suresh G.; Banumathy B.; Masilamani S.;
Gopalakrishnan G.; KrishnaKumari G.N. Antifungal activity of some
B, D-seco limonoids from two meliaceous plants. Journal of Chemical
Ecology,1999, 25(4): 923-933.
[6] Li, D., Chen, J.H., Chen, Q., Li., G.W, Chen, J., Yue, J.M., Chen, M.L.,
Wang, X.P., Shen, J.H., Shen, X., Jiang, H.L. Swietenia mahagony
extract shows agonistic activity to PPAR and gives ameliorative effects
on diabetic db/db mice. Acta Pharmacologica Sinica, 2005, 26(2): 220-
222.
[7] Nagalakshmi, M.; Thangadurai, D.; Muralidara, D.; Pullaiah, R.T.
Phytochemical and antimicrobial study of Chukrasia tabularis leaves.
Fitoterapia, 2001, 72(1): 62-64.
[8] Bacsal, K.; Havez, L.; Diaz, I.; Espina, S.; Javillo, J.; Manzanilla, H.;
Motalban, J.; Panganiban, C.; Ro driguez, A.; Sumpaico, C.; Talip, B.;
Yap, S. The effect of Switenia mahogany (mahogany) seed extract on
indomethacin-induced gastric ulcers in female Sprague-Dawley rats.
Acta Medica Philippina, 1997, 3:127-139.
[9] Sahgal, G., Ramanathan S, Sasidharan S, Mordi MN, Ismail S, Mansor
SM. In Vitro Antioxidant and Xanthine Oxidase Inhibitory Activities of
Methanolic Swietenia mahagoni Seed Extracts. Molecules (Basel,
Switzerland), 2009, 14(11): 4476-85.
[10] Sahgal, G., Ramanathan, S., Sasidharan, S., Mordi, M.N., Ismail, S. and
Mansor, S.M. Phytochemical and antimicrobial activity of Swietenia
mahagoni crude methanolic seed extract. Tropical Biomedicine,2009,
26(3): 274-279.
[11] Li, X., Andersson, T.B., Ahlstom, M., Weidolf, L. Comparison of
inhibitory effects of the proton pump-inhibiting drugs omeprazole,
esomeprazole, lansoprazole, pantoprazole, and rabeprazole on human
cytochrome P450 activities. Drug metabolism and disposition, 2004,
32(8): 821-827.
[12] Satoh, H., Inatomi, N., Nagaya, H., Ianda, I., Nohara, A., Nakamura, H.
Antisecretory and antiulcer activities of a novel proton pump inhibitor
AG-1749 in dogs and rats. Journal of Pharmacology and Experimental
Therapeutics, 1989, 248(2): 806-815.
[13] Nagaya, H., Inatomi, N., Ohara, A., Satoh, H. (1991). Effects of the
enantiomers of lansoprazole (AG-1749) on (H++ K+)-ATPase activity
in canine gastric microsomes and acid formation in isolated canine
parietal cells. Biochemical pharmacology, 42(10): 1875-1878.
[14] Pedernera, A.M., Guardia, T., Caleron, C.G., Rotelli, A.F., de la Rocha,
N.F., di Genaro, S., Pelze, L.F. Anti-ulcerogenic and anti-inflammatory
activity of the methanolic extract of Larrea divaricata Cav. in rat.
Journal of ethnopharmacology, 2006, 105(3): 415-420.
[15] De Pasquale, R., Germano, M.P., Keita, A., Sanogo, R., Lauk, L.
Antiulcer activity of Pteleopsis suberosa. Journal of
ethnopharmacology, 1995, 47(1): 55-58.
[16] Bergmeyer, H. and M. Horder. IFCC methods for the measurement of
catalytic concentration of enzymes, Part 3. IFCC method for alanine
aminotransferase. J Clin Chem Clin Biochem, 1980, 18: 521-534.
[17] Tietz, N.W., Rinker, A.D., Shaw, L.M. (1983). IFCC methods for the
measurement of catalytic concentration of enzymes, Part 5. IFCC
method for alkaline phosphatase. J Clin Chem Clin Biochem, 1983,
21(11): 731-748.
[18] Garg, G.P., Nigam, S.K., Ogle, C.W. The gastric antiulcer effects of the
leaves of the neem tree. Planta medica, 1993, 59: 215-215.
[19] Hollander, D., Taranawski, A., Krause, W.J., Gergely, H. Protective
effect of sucralfate against alcohol-induced gastric mucosal injury in
the rat. Macroscopic, histologic, ultrastructural, and functional time
sequence analysis. Gastroenterology, 1985, 88(1 Pt 2): 366-374.
[20] Paiva, L.A.F, Rao, V.S.N., Gramosa, N.V., Silveira, F.R.
Gastroprotective effect of Copaifera langsdorffii oleo-resin on
experimental gastric ulcer models in rats. Journal of
ethnopharmacology, 1998, 62(1): 73-78.
[21] Kauffman, G. L., Grossman, M. I. Prostaglandin and cimetidine inhibit
the formation of ulcers produced by parenteral salicylates.
Gastroenterology, 1978, 75(6): 1099-2102.
[22] Njar V. C. O., Adesanwo J. K., Raji Y. Methyl Angolenate: The
Antiulcer Agent from the Stem Bark of Entandrophragma angolense.
Planta medica, 1995, 61: 91-92.
[23] Piper, D.W. and Stiel, D. (1986). Pathogenesis of chronic peptic ulcer,
current thinking and clinical implications. Med Prog, 2: 7-10.
[24] Baron, T.H., Langman, M.T.S. and Wastell, C. Stomach and
duodenum. Recent advances in gastroenterology. Churchill
Livingstone, Edinburgh, 1980, p. 23-86.
[25] Szabo, S., Kusstatscher, S, Sakoulas, G, Sandor, Z, Vincze, A & Jadus,
M. Growth factors: new ÔÇÿendogenous drugs- for ulcer healing.
Scandinavian Journal of Gastroenterology, 1995, 30: p. 15-18.
[26] Marhuenda, E., Martin, M.J. and Alarcon de la Lastra, C.
Antiulcerogenic activity of aescine in different experimental models.
Phytotherapy Research, 2006, 7(1): 13-16.
[27] Mutoh, H., Hiraishi, H., Ota, S., Ivey, K.J. Terano, A. Sugimoto, T.
Role of oxygen radicals in ethanol-induced damage to cultured gastric
mucosal cells. American Journal of Physiology- Gastrointestinal and
Liver Physiology, 1990, 258(4): G603-G609.
[28] Trivedi, N.P., Rawal, U.M. Hepatoprotective and antioxidant property
of Andrographis paniculata (Nees) in BHC induced liver damage in
mice. Indian Journal of Experimental Biology, 2001, 39(1): 41-46.
[29] Ligumsky, M., Sestieri, M., Okon, F., Ginsburg, I. Antioxidants inhibit
ethanol-induced gastric injury in the rat: role of manganese, glycine,
and carotene. Scandinavian Journal of Gastroenterology, 1995, 30(9):
854-860.
[30] Kobayashi, T., Ohta, Y., Yoshino, J., Nakazawa, S. Teprenone
promotes the healing of acetic acid-induced chronic gastric ulcers in
rats by inhibiting neutrophil infiltration and lipid peroxidation in
ulcerated gastric tissues. Pharmacological Research, 2001, 43(1): 23-
30.
[31] Shimizu, N., Watanabe T, Arakawa T, Fujiwara Y, Higuchi K, Kuroki
T. Pentoxifylline accelerates gastric ulcer healing in rats: roles of tumor
necrosis factor alpha and neutrophils during the early phase of ulcer
healing. Digestion, 2000, 61(3): 157-164.
[32] Cheng CL, Koo MWL. Effects of Centella asiatica on ethanol induced
gastric mucosal lesions in rats. Life sciences, 2000, 67(21): 2647-2653.
[33] Fujita, H, Takahashi, S., Okabe, S. Mechanism by which indomethacin
delays the healing of acetic acid-induced ulcers in rats. Role of
neutrophil antichemotactic and chemotactic activities. Journal of
physiology and pharmacology: an official journal of the Polish
Physiological Society, 1998, 49(1): 71-82.
[34] Suzuki, Y., Ishihara M, Ito M. Anti-ulcer effects of antioxidants,
quercetin, -tocopherol, nifedipine and tetracycline in rats. The Japanese
Journal of Pharmacology, 1998, 78(4): p. 435-441.
[35] Zimmerman, J.J. Ciesielski, W., Lewandoski, J. Neutrophil-mediated
phospholipid peroxidation assessed by gas chromatography-mass
spectroscopy. American Journal of Physiology- Cell Physiology, 1997,
273(2): C653-661.
[36] Garrick, T., Buack, S., Bass, P. Gastric motility is a major factor in
cold restraint-induced lesion formation in rats. American Journal of
Physiology- Gastrointestinal and Liver Physiology, 1986, 250(2):191-
199.
[37] Takeuchi, K., Nishiwakki, K., Okabe, S. Effects of dopamine on gastric
mucosal lesions induced by ethanol in rats. Digestive Diseases and
Sciences, 1988, 33(12): 1560-1568.
[38] Takeuch, K. and Nobuhara, Y. Inhibition of gastric motor activity by
16, 16-dimethyl prostaglandin E 2. Digestive Diseases and Sciences,
1985, 30(12): 1181-1188.
[39] Mersereau, A.W. and Hinchey, E.J. Role of gastric mucosal folds in
formation of focal ulcers in the rat. Surgery, 1982, 91(2):150-155.
@article{"International Journal of Medical, Medicine and Health Sciences:64748", author = "Salma Saleh Alrdahe and Mahmood Ameen Abdulla and Shaharudin Abul Razak and Farkaad Abdul Kadir and Pouya Hassandarvish", title = "Gastroprotective Activity of Swietenia Mahagoni Seed Extract on Ethanol-Induced Gastric Mucosal Injury in Rats", abstract = "Swietenia mahagoni have been used in traditional
medicine for treatment of different diseases. Present study was
performed to evaluate anti-ulcerogenic activity of ethanol seed
extract against ethanol induced gastric mucosal injury in rats. Six
groups of rats were orally pre-treated respectively with
carboxymethyl cellulose, omeprazole 20 mg/kg, 50, 100, 200 and 400
mg/kg plant extract one hour before oral administration of absolute
ethanol to generate gastric mucosal injury. After additional hour, rats
were sacrificed and ulcer areas of gastric walls were determined.
Grossly, carboxymethyl cellulose group exhibited severe mucosal
injury, whereas pre-treatment with plant extract exhibited significant
protection of gastric mucosa. Histology, carboxymethyl cellulose
group exhibited severe damage of gastric mucosa; edema and
leucocytes infiltration of sub mucosa compared to plant extract which
showed gastric protection. Acute toxicity study did not manifest any
toxicological signs in rats. Conclusions, results suggest that S.
mahagoni promotes ulcer protection as ascertained grossly and
histologically.", keywords = "Cytoprotection, Gastric ulcer, Histology, Swieteniamahagoni seed.", volume = "4", number = "7", pages = "304-5", }
