Abstract: In this study, the ability of Aspergillus niger and
Penicillium simplicissimum to extract heavy metals from a spent
refinery catalyst was investigated. For the first step, a spent
processing catalyst from one of the oil refineries in Iran was
physically and chemically characterized. Aspergillus niger and
Penicillium simplicissimum were used to mobilize Al/Co/Mo/Ni from
hazardous spent catalysts. The fungi were adapted to the mixture of
metals at 100-800 mg L-1 with increments in concentration of 100 mg
L-1. Bioleaching experiments were carried out in batch cultures. To
investigate the production of organic acids in sucrose medium,
analyses of the culture medium by HPLC were performed at specific
time intervals after inoculation. The results obtained from Inductive
coupled plasma-optical emission spectrometry (ICP-OES) showed
that after the one-step bioleaching process using Aspergillus niger,
maximum removal efficiencies of 27%, 66%, 62% and 38% were
achieved for Al, Co, Mo and Ni, respectively. However, the highest
removal efficiencies using Penicillium simplicissimum were of 32%,
67%, 65% and 38% for Al, Co, Mo and Ni, respectively
Abstract: An organic bulk heterojunction (BHJ) was fabricated using a blended film containing Copper (II) tetrakis(4-acumylphenoxy) phthalocyanine (Tc-CuPc) along with [6,6]-Phenyl C61 butyric acid methyl ester (PCBM). Weight ratio between Tc-CuPc and PCBM was 1:1. The electrical properties of Tc-CuPc: PCBM BHJ were examined. Rectifying nature of the BHJ was displayed by current-voltage (I-V) curves, recorded in dark and at various temperatures. At low voltages, conduction was ohmic succeeded by space-charge limiting current (SCLC) conduction at higher voltages in which exponential trap distribution was dominant. Series resistance, shunt resistance, ideality factor, effective barrier height and mobility at room temperature were found to be 526 4, 482 k4, 3.7, 0.17 eV and 2×10-7 cm2V-1s-1 respectively. Temperature effect towards different BHJ parameters was observed under dark condition.
Abstract: The composition, vapour pressure, and heat capacity
of nine biodiesel fuels from different sources were measured. The
vapour pressure of the biodiesel fuels is modeled assuming an ideal
liquid phase of the fatty acid methyl esters constituting the fuel. New
methodologies to calculate the vapour pressure and ideal gas and
liquid heat capacities of the biodiesel fuel constituents are proposed.
Two alternative optimization scenarios are evaluated: 1) vapour
pressure only; 2) vapour pressure constrained with liquid heat
capacity. Without physical constraints, significant errors in liquid
heat capacity predictions were found whereas the constrained
correlation accurately fit both vapour pressure and liquid heat
capacity.
Abstract: Sulphur dioxide is a harmful gaseous product that
needs to be minimized in the atmosphere. This research work
investigates the use of zeolite as a possible additive that can improve
the sulphur dioxide capture in wet flue gas desulphurisation
dissolution process. This work determines the effect of temperature,
solid to liquid ratio, acid concentration and stirring speed on the
leaching of zeolite using a pH stat apparatus. The atomic absorption
spectrometer was used to measure the calcium ions from the solution.
It was found that the dissolution rate of zeolite decreased with
increase in solid to liquid ratio and increases with increase in
temperature, stirring speed and acid concentration. The activation
energy for the dissolution rate of zeolite in hydrochloric acid was
found to be 9.29kJ/mol. and therefore the product layer diffusion was
the rate limiting step.
Abstract: In April 2009, a new variant of Influenza A virus
subtype H1N1 emerged in Mexico and spread all over the world. The
influenza has three subtypes in human (H1N1, H1N2 and H3N2)
Types B and C influenza tend to be associated with local or regional
epidemics. Preliminary genetic characterization of the influenza
viruses has identified them as swine influenza A (H1N1) viruses.
Nucleotide sequence analysis of the Haemagglutinin (HA) and
Neuraminidase (NA) are similar to each other and the majority of
their genes of swine influenza viruses, two genes coding for the
neuraminidase (NA) and matrix (M) proteins are similar to
corresponding genes of swine influenza. Sequence similarity between
the 2009 A (H1N1) virus and its nearest relatives indicates that its
gene segments have been circulating undetected for an extended
period. Nucleic acid sequence Maximum Likelihood (MCL) and
DNA Empirical base frequencies, Phylogenetic relationship amongst
the HA genes of H1N1 virus isolated in Genbank having high
nucleotide sequence homology.
In this paper we used 16 HA nucleotide sequences from NCBI for
computing sequence relationships similarity of swine influenza A
virus using the following method MCL the result is 28%, 36.64% for
Optimal tree with the sum of branch length, 35.62% for Interior
branch phylogeny Neighber – Join Tree, 1.85% for the overall
transition/transversion, and 8.28% for Overall mean distance.
Abstract: Certain tRNA synthetases have developed highly accurate molecular machinery to discriminate their cognate amino acids. Those aaRSs achieve their goal via editing reaction in the Connective Polypeptide 1 (CP1). Recently mutagenesis studies have revealed the critical importance of residues in the CP1 domain for editing activity and X-ray structures have shown binding mode of noncognate amino acids in the editing domain. To pursue molecular mechanism for amino acid discrimination, molecular modeling studies were performed. Our results suggest that aaRS bind the noncognate amino acid more tightly than the cognate one. Finally, by comparing binding conformations of the amino acids in three systems, the amino acid binding mode was elucidated and a discrimination mechanism proposed. The results strongly reveal that the conserved threonines are responsible for amino acid discrimination. This is achieved through side chain interactions between T252 and T247/T248 as well as between those threonines and the incoming amino acids.
Abstract: Using plug flow model in conjunction with
experimental solute concentration profiles, overall volumetric mass
transfer coefficient based on continuous phase (Koca), in a packed
liquid-liquid extraction column has been optimized. Number of 12
experiments has been done using standard system of water/acid
acetic/toluene in a 6 cm diameter, 120 cm height column. Thorough
consideration of influencing parameters we intended to correlate
dimensionless parameters in term of overall Sherwood number which
has an acceptable average error of about 15.8%.
Abstract: The amount of the information being churned out by the field of biology has jumped manifold and now requires the extensive use of computer techniques for the management of this information. The predominance of biological information such as protein sequence similarity in the biological information sea is key information for detecting protein evolutionary relationship. Protein sequence similarity typically implies homology, which in turn may imply structural and functional similarities. In this work, we propose, a learning method for detecting remote protein homology. The proposed method uses a transformation that converts protein sequence into fixed-dimensional representative feature vectors. Each feature vector records the sensitivity of a protein sequence to a set of amino acids substrings generated from the protein sequences of interest. These features are then used in conjunction with support vector machines for the detection of the protein remote homology. The proposed method is tested and evaluated on two different benchmark protein datasets and it-s able to deliver improvements over most of the existing homology detection methods.
Abstract: Empirical force fields and density functional theory
(DFT) was used to study the binding energies and structures of
methylamine on the surface of activated carbons (ACs). This is a first
step in studying the adsorption of alkyl amines on the surface of
functionalized ACs. The force fields used were Dreiding (DFF),
Universal (UFF) and Compass (CFF) models. The generalized
gradient approximation with Perdew Wang 91 (PW91) functional
was used for DFT calculations. In addition to obtaining the aminecarboxylic
acid adsorption energies, the results were used to establish
reliability of the empirical models for these systems. CFF predicted a
binding energy of -9.227 (kcal/mol) which agreed with PW91 at -
13.17 (kcal/mol), compared to DFF 0 (kcal/mol) and UFF -0.72
(kcal/mol). However, the CFF binding energies for the amine to ester
and ketone disagreed with PW91 results. The structures obtained
from all models agreed with PW91 results.
Abstract: This article introduces the actual problem that is а
study of proposed by the authors Hydrocele ointment in amino acids’
metabolism of cows’ blood in inflammation of traumatic origin.
Hydrocele ointment has shown a positive effect on inflammatory
process and amino acids’ metabolism of animals treated with the
drug. Amino acid levels reached physiological parameters on the 10th
day after treatment; in the control group this parameter was higher
than normal.
Abstract: Extraction of lactic acid by emulsion liquid membrane technology (ELM) using n-trioctyl amine (TOA) in n-heptane as carrier within the organic membrane along with sodium carbonate as acceptor phase was optimized by using response surface methodology (RSM). A three level Box-Behnken design was employed for experimental design, analysis of the results and to depict the combined effect of five independent variables, vizlactic acid concentration in aqueous phase (cl), sodium carbonate concentration in stripping phase (cs), carrier concentration in membrane phase (ψ), treat ratio, and batch extraction time (τ)
with equal volume of organic and external aqueous phase on lactic acid extraction efficiency. The maximum lactic acid extraction efficiency (ηext) of 98.21%from aqueous phase in a batch reactor using ELM was found at the optimized values for test variables, cl, cs, ψ, and τ as 0.06 [M], 0.18 [M], 4.72 (%,v/v), 1.98 (v/v) and 13.36 min respectively.
Abstract: The objective of this work is to produce heterotrophic
microalgal lipid in flask-batch fermentation. Chlorella sp. KKU-S2
supported maximum values of 0.374 g/L/d, 0.478 g lipid/g cells, and
0.112 g/L/d for volumetric lipid production rate, and specific yield of
lipid, and specific rate of lipid production, respectively when culture
was performed on BG-11 medium supplemented with 50g/L glucose.
Among the carbon sources tested, maximum cell yield coefficient
(YX/S, g/L), maximum specific yield of lipid (YP/X, g lipid/g cells) and
volumetric lipid production rate (QP, g/L/d) were found of 0.728,
0.237, and 0.619, respectively, using sugarcane molasses as carbon
source. The main components of fatty acid from extracted lipid were
palmitic acid, stearic acid, oleic acid and linoleic acid which similar
to vegetable oils and suitable for biodiesel production.
Abstract: Oleic acid (C18:1) play an important role in
proliferation of fat cells. In this study, the effect of oleate on cells
viability in 3T3-L1 cells (fat cells) was investigated. The 3T3-L1
cells were treated with various concentrations of oleate in the
presence of 23 mM glucose. Oleate was added to adipogenic media
(day 0) to investigate the influence of oleate on proliferation of
postconfluent preadipocytes after 24 h induction. 0.1 mM oleate
promoted cell division by increasing 33.9% number of cells from
basal control in postconfluent preadipocytes. However, there were no
significantly different in cells viability with control cells when oleate
concentrations were increased up to 0.5 mM. When added to
differentiated adipocytes (day 12) for 48 h, the number of cells
decreased as oleate concentrations increased. 92.7% of cells lost
demonstrated apoptosis and necrosis after 48 h with 0.5 mM oleate.
The fluorochrome staining was examined under fluorescence
microscopy using acridine orange and ethidium bromide double
staining. Furthermore, the presence of high lactate (60.6% increased
from basal control) released into plasma has shown the direct
cytotoxicity of 0.5 mM oleate on adipocytes.
Abstract: Rice bran has been abandoned as agricultural waste for million tonnes per year in Thailand, therefore they have been proposed to be utilized as a rich carbon source in the production of bioethanol. Many toxic compounds are possibly released during the pretreatment of rice bran prior the fermentation process. This study aims to analyze on the availability of toxic compounds and the amount of glucose obtained from 2 different pretreatments using sulfuric acid and mixed cellulase enzymes (without and with delignification/ activated charcoal). The concentration of furfural, 5- hydroxymethyl furfural (5-HMF), levulinic acid, vanillin, syringaldehyde and4-hydroxybenzaldehyde (4-HB) and the percent acetic acid were found to be 0.0517 ± 0.049 mg/L, 0.032 ± 0.06 mg/L, 21074 ± 1685.62 mg/L, 126.265 ± 6.005 mg/L, 2.89 ± 0.30 mg/L, 0.37 ± 0.031mg/L and 0.72% under the pretreatment process without delignification/ activated charcoal treatment and 384.47 ± 99.02 g/L, 0.068 mg/L, 142107.62 ± 8664.6 mg/L, 0.19 mg/L, 5.43 ± 3.29 mg/L, 4.80 ± 0.76 mg/L and 0.254% under the pretreatment process with delignification/ activated charcoal treatment respectively. The presence of high concentration of acetic acid was found to impede the growth of Zymomonas mobilis strain TISTR 551 despite the present of high concentration of levulinic acid. Z. mobilis strain TISTR 551 was found to produce 8.96 ± 4.06 g/L of ethanol under 4 days fementation period in biofilm stage in which represented 40% theoretical yield.
Abstract: The potential of antioxidant activities of the plant
extract Gynura procumbens, Achyranthes aspera and Polygenum
tomentosum were studied by using 1, 1-diphenyl-2-picrylhydrazyl
(DPPH) .Antioxidant activity was qualitatively and quantitatively
determined. In this analysis , Ascorbic acid (Vitamin C) was used as
the standard .The antioxidant activities were observed all three plant
extracts and the EC50 values of G procumbens A.aspera and
P.tomemtosum were 13.7 μg /ml,14.37 μg /ml and 14.35 μg /ml.
Among these plants, G.procumbens is more potent antioxidant
activity then others. Antitumor activities were found with A.aspera
(s2) extracts in the dose of 100ppm in carrot disks and G.procumbens
(s1) and P.tomentosum (s3) in the dose of 1000 ppm. Therefore, these
herbal plants are used in traditional medicines.
Abstract: A new supported liquid membrane (SLM) system for
the selective transport of VO2
+ ions was prepared in this present
work. The SLM was a thin porous polyvinylidene difluoride
(PVDF) membrane soaked with Di-(2-ethylhexyl) phosphoric acid
(D2EHPA) as mobile carrier in Xylene as organic solvent.
D2EHPA acts as a highly selective carrier for the uphill transport of
VO2
+ ions through the SLM. The transport of VO2
+ ions reached to
64%. In the presence of P2O7-2 ion as suitable masking agent in the
feed solution, the interfering effects of other cations were eliminated.
Abstract: Supercritical carbon dioxide (SC-CO2) was used as a
solvent to extract oil from wheat bran. Extractions were carried out in a
semi-batch process at temperatures ranging from 40 to 60ºC and
pressures ranging from 10 to 30 MPa, with a carbon dioxide (CO2)
flow rate of 26.81 g/min. The oil obtained from wheat bran at different
extraction conditions was quantitatively measured to investigate the
solubility of oil in SC-CO2. The solubility of wheat bran oil was found
to be enhanced in high temperature and pressure. The composition of
fatty acids in wheat bran oil was measured by gas chromatography
(GC). Linoleic, palmitic, oleic and γ-linolenic acid were the major
fatty acids of wheat bran oil. Tocopherol contents in oil were analyzed
by high performance liquid chromatography (HPLC). The highest
amount of phenolics and tocopherols (α and β) were found at
temperature of 60ºC and pressure of 30 MPa.
Abstract: An efficient and green protocol for the synthesis of α-
aminonitriles derivatives by one-pot reaction of different aldehydes
with amines and trimethylsilyl cyanides has been developed using
natural alumina, alumina sulfuric acid (ASA), nano-γ-alumina, nanoalumina
sulfuric acid (nano-ASA) under microwave irradiation and
solvent-free conditions. The advantages of methods are short reaction
times, high yields, milder conditions and easy work up. The catalysts
can be recovered for the subsequent reactions and reused without any
appreciable loss of efficiency.
Abstract: Microorganisms isolated from water and soil of
Kazakhstan to identify potential high-effective producers of the
arachidonic acid, exhibiting a wide range of physiological activity
and having practical applications were screened. Based on the results
of two independent tests (the test on the sensitivity of the growth
processes of microorganisms to acetylsalicylic acid - an irreversible
inhibitor of PGH-synthase involved in the metabolism of arachidonic
acid and its derivatives, the test for inhibition of peroxidase activity
of membrane-bounding fraction of PGH - synthase by acetylsalicylic
acid) were selected microbial cultures which are potential highproducer
of arachidonic acid. They are characterized by a stable
strong growth in the laboratory conditions. Identification of
microorganism cultures based on morphological, physiological,
biochemical and molecular genetic characteristics was performed.
Abstract: The dilute acid pretreatment and enzymatic
saccharification of lignocellulosic substrate, cogon grass (Imperata
cylindrical, L.) was optimized prior ethanol fermentation using
simultaneous saccharification and fermentation (SSF) method. The
optimum pretreatment conditions, temperature, sulfuric acid
concentration, and reaction time were evaluated by determining the
maximum sugar yield at constant enzyme loading. Cogon grass, at
10% w/v substrate loading, has optimum pretreatment conditions of
126°C, 0.6% v/v H2SO4, and 20min reaction time. These
pretreatment conditions were used to optimize enzymatic
saccharification using different enzyme combinations. The maximum
saccharification yield of 36.68mg/mL (71.29% reducing sugar) was
obtained using 25FPU/g-cellulose cellulase complex combined with
1.1% w/w of cellobiase, ß-glucosidase, and 0.225% w/w of
hemicellulase complex, after 96 hours of saccharification. Using the
optimum pretreatment and saccharification conditions, SSF of treated
substrates was done at 37°C for 120 hours using industrial yeast
strain HBY3, Saccharomyces cerevisiae. The ethanol yield for cogon
grass at 4% w/w loading was 9.11g/L with 5.74mg/mL total residual
sugar.