Microalgal Lipid Production by Microalgae Chlorella sp. KKU-S2
The objective of this work is to produce heterotrophic
microalgal lipid in flask-batch fermentation. Chlorella sp. KKU-S2
supported maximum values of 0.374 g/L/d, 0.478 g lipid/g cells, and
0.112 g/L/d for volumetric lipid production rate, and specific yield of
lipid, and specific rate of lipid production, respectively when culture
was performed on BG-11 medium supplemented with 50g/L glucose.
Among the carbon sources tested, maximum cell yield coefficient
(YX/S, g/L), maximum specific yield of lipid (YP/X, g lipid/g cells) and
volumetric lipid production rate (QP, g/L/d) were found of 0.728,
0.237, and 0.619, respectively, using sugarcane molasses as carbon
source. The main components of fatty acid from extracted lipid were
palmitic acid, stearic acid, oleic acid and linoleic acid which similar
to vegetable oils and suitable for biodiesel production.
[1] Fukuda, H., Kondo, A. and Noda, H. (2001) Biodiesel fuel production by
transesterification of oils. J Biosci Bioeng 92:405-416.
[2] Chisti, Y. (2007) Biodiesel from microalgae. Biotechnol Adv 25: 294-
306.
[3] Amin, S. (2009) Review on biofuel oil and gas production processes
from microalgae. Energy Conversion and Management 50:1834-1840.
[4] Huang, G.H., Chen, F., Wei, D., Zhang, X.W., and Chen, G. (2010)
Biodiesel production by microalgal biotechnology. Appl Energy 87:38-
46.
[5] Miao, X., and Wu, Q. (2006) Biodiesel production from heterotrophic
microalgal oil. Biores Technol 97:841-846.
[6] Lee, J.M. (1992) Biochemical Engineering. Prentice Hall international,
New Jersey.
[7] Kwon, D.Y. and Rhee, J.S. (1986) A Simple and rapid colorimetric
method for determination of free fatty acids for lipase assay. J Am Oil
Chem Soc 63:89-92.
[8] Lepage, G., and Roy, C.C. (1984) Improved recovery of fatty acid
through direct transesterification without prior extraction or purification.
J Lipid Res 25:1391-1396
[9] Xiong, W., Li, X., Xiang, J., and Wu, Q. (2008) High-density
fermentation of microalga Chlorella protothecoides in bioreactor for
microbio-diesel production. Appl Microbiol Biotechnol 78:29-36
[1] Fukuda, H., Kondo, A. and Noda, H. (2001) Biodiesel fuel production by
transesterification of oils. J Biosci Bioeng 92:405-416.
[2] Chisti, Y. (2007) Biodiesel from microalgae. Biotechnol Adv 25: 294-
306.
[3] Amin, S. (2009) Review on biofuel oil and gas production processes
from microalgae. Energy Conversion and Management 50:1834-1840.
[4] Huang, G.H., Chen, F., Wei, D., Zhang, X.W., and Chen, G. (2010)
Biodiesel production by microalgal biotechnology. Appl Energy 87:38-
46.
[5] Miao, X., and Wu, Q. (2006) Biodiesel production from heterotrophic
microalgal oil. Biores Technol 97:841-846.
[6] Lee, J.M. (1992) Biochemical Engineering. Prentice Hall international,
New Jersey.
[7] Kwon, D.Y. and Rhee, J.S. (1986) A Simple and rapid colorimetric
method for determination of free fatty acids for lipase assay. J Am Oil
Chem Soc 63:89-92.
[8] Lepage, G., and Roy, C.C. (1984) Improved recovery of fatty acid
through direct transesterification without prior extraction or purification.
J Lipid Res 25:1391-1396
[9] Xiong, W., Li, X., Xiang, J., and Wu, Q. (2008) High-density
fermentation of microalga Chlorella protothecoides in bioreactor for
microbio-diesel production. Appl Microbiol Biotechnol 78:29-36
@article{"International Journal of Biological, Life and Agricultural Sciences:50900", author = "Ratanaporn Leesing and Supaporn Kookkhunthod and Ngarmnit Nontaso", title = "Microalgal Lipid Production by Microalgae Chlorella sp. KKU-S2", abstract = "The objective of this work is to produce heterotrophic
microalgal lipid in flask-batch fermentation. Chlorella sp. KKU-S2
supported maximum values of 0.374 g/L/d, 0.478 g lipid/g cells, and
0.112 g/L/d for volumetric lipid production rate, and specific yield of
lipid, and specific rate of lipid production, respectively when culture
was performed on BG-11 medium supplemented with 50g/L glucose.
Among the carbon sources tested, maximum cell yield coefficient
(YX/S, g/L), maximum specific yield of lipid (YP/X, g lipid/g cells) and
volumetric lipid production rate (QP, g/L/d) were found of 0.728,
0.237, and 0.619, respectively, using sugarcane molasses as carbon
source. The main components of fatty acid from extracted lipid were
palmitic acid, stearic acid, oleic acid and linoleic acid which similar
to vegetable oils and suitable for biodiesel production.", keywords = "Microalgal lipid, Chlorella sp. KKU-S2, kineticparameters, biodiesel.", volume = "5", number = "4", pages = "198-4", }