Production of Glucose from the Hydrolysis of Cassava Residue using Bacteria Isolates from Thai Higher Termites

The possibility of using cassava residue containing 49.66% starch, 21.47% cellulose, 12.97% hemicellulose, and 21.86% lignin as a raw material to produce glucose using enzymatic hydrolysis was investigated. In the experiment, each reactor contained the cassava residue, bacteria cells, and production medium. The effects of particles size (40 mesh and 60 mesh) and strains of bacteria (A002 and M015) isolated from Thai higher termites, Microcerotermes sp., on the glucose concentration at 37°C were focused. High performance liquid chromatography (HPLC) with a refractive index detector was used to determine the quantity of glucose. The maximum glucose concentration obtained at 37°C using strain A002 and 60 mesh of the cassava residue was 1.51 g/L at 10 h.

[1] Chang, K.L., Thitikorn-amorn, J., Hsieh, J.F., Ou, B.M., Chen, S.H.,
Ratanakhanokchai, K., Huang, P.J., and Chen, S.T. (2011). Enhanced
enzymatic conversion with freeze pretreatment of rice straw. Biomass
and Biology, 35(1), 90 - 95.
[2] Gonzalez, J.F., Rayo, M.C., Roman, S., Gonzalez-Garcia, C.M. and
Ledesma, B. (2010). Modelling non-isothermal degradation of olives
solid waste: influence of variables and kinetics, third international
symposium on energy from biomass and waste. CISA, Environmental
Sanitary Engineering Centre, Italy
[3] Mussatto, S.I. and Teixeira, J.A. (2010). Lignocellulose as raw material
in fermentation processes. Applied Microbiology and Microbial
Biotechnology, 897 - 907.
[4] Pandey, A., Soccol, C.R., Nigam, P., Soccol, V.T., Vandenberghe,
L.P.S. and Mohan, R. (2000). Biotechnological potential of agroindustrial
residues. II: cassava bagasse. Bioresources Technology,
74(1), 81 - 87.
[5] Nair, M.P.D., Padmaja, G. and Moorthy, S.N. (2011). Biodegradation
of cassava starch factory residue and using a combination of cellulases,
xylanases and hemicellulases. Biomass and Bioenergy, 35(1), 1211 -
[6] Sun, Y. and Cheng, J. (2002). Hydrolysis of lignocellulosic materials
for ethanol production: a review. Bioresource Technology, 83(1), 1 -
[7] Soares, I.B., Travassos, J.A., Baudel, H.M., Benachour, M. and Abreu,
C.A.M. (2011). Effects of washing, milling and loading enzymes on the
enzymatic hydrolysis of a steam pretreated sugarcane bagasse.
Industrial Crops and Products, 33(3), 670 - 675.
[8] Taechapoempol, K., Sreethawong, T., Rangsunvigit, P., Namprohm, W.,
Thamprajamchit, B., Rengpipat, S., Chavadej, S. (2010). Cellulase-
Producing Bacteria from Thai Higher Termites, Microcerotermes sp.:
Enzymatic Activities and Ionic Liquid Tolerance. Biochem
Biotechnology, 164, 204-219.
[9] Eourarekullart, W. (2011). Conversion of corncob to sugars by
microbial hydrolysis. M.S. Thesis, The Petroleum and Petrochemical
College, Chulalongkorn University.
[10] Yeh, A., Huang, Y., and Chen, S.H. (2010) Effect of particle size on the
rate of enzymatic hydrolysis of cellulose. Carbohydrate Polymers, 79(1),