Kinetic Studies on Microbial Production of Tannase Using Redgram Husk
Tannase (tannin acyl hydrolase, E.C.3.1.1.20) is an
important hydrolysable enzyme with innumerable applications and
industrial potential. In the present study, a kinetic model has been
developed for the batch fermentation used for the production of
tannase by A.flavus MTCC 3783. Maximum tannase activity of
143.30 U/ml was obtained at 96 hours under optimum operating
conditions at 35oC, an initial pH of 5.5 and with an inducer tannic
acid concentration of 3% (w/v) for a fermentation period of 120
hours. The biomass concentration reaches a maximum of 6.62 g/l at
96 hours and further there was no increase in biomass concentration
till the end of the fermentation. Various unstructured kinetic models
were analyzed to simulate the experimental values of microbial
growth, tannase activity and substrate concentration. The Logistic
model for microbial growth , Luedeking - Piret model for production
of tannase and Substrate utilization kinetic model for utilization of
substrate were capable of predicting the fermentation profile with
high coefficient of determination (R2) values of 0.980, 0.942 and
0.983 respectively. The results indicated that the unstructured models
were able to describe the fermentation kinetics more effectively.
[1] C. N Aguilar, and G. Gutiérrez-Sánchez, “Review: sources, properties,
applications and potential uses of tannin acyl hydrolase,” Food Sci.
Technol. Int, vol.7, pp. 373-382, 2001.
[2] A. A Esener, J. A Roels, N. W Kossen,”Theory and applications of
unstructured growth models: Kinetic and energetic aspects,” Biotechnol
Bioeng, vol. 25, pp.2803-2841, 1983.
[3] A.E Ghaly, M. Kamal, L. R. Correia,”Kinetic modeling of continuous
submerged fermentation of cheese whey for single cell protein
production,” Bioresource Technology, vol. 96, pp. 1143-1152, 2005.
[4] A E. Haggerman, L G. Butler. “Protein precipitation method for
determination of tannins,” J. Agric. Food Chem., vol.26, pp.809–812,
1978.
[5] E. Haslam, J E. Stangroom, “The esterase and depsidase activities of
tannase,” Biochem. J., vol.99, pp.28-31, 1966.
[6] P K. Lekha and B K. Lonsane, “Production and application of tannin
acyl hydrolase; state of the art,” Advances in Applied Microbiology, vol.
44, pp.215–260, 1997.
[7] W. Madhavakrishna, S M. Bose, Y. Nayudamma, “Estimation of tannase
and certain oxidizing enzymes in Indian vegetable tanstuffs,” Bull. Cent.
Leath. Res. Instit, vol. 7, pp. 1-11, 1960.
[8] K. C. Mondal, D. Banerjee, M. Jana and B. R. Pati, “Calorimetric assay
method for determination of the tannase activity,” Analytical
Biochemistry, vol. 295, pp. 168-171, 2001.
[9] J. Monod, “The growth of Bacterial cultures,” Annual Rev Microbiol,
vol.3, pp- 371-394, 1949.
[10] R A Moraine, P Rogovin,” Xanthan biopolymer production at increased
concentration by pH control,” Biotechnol Bioengg, vol.13, pp.381-391,
1971.
[11] G. Narasimha, A. Sridevi, B. Viswanath, M. Subhosh Chandra, B.
Rajasekarreddy, “Nutrient effects on production of cellulytic enzymes
by A. niger,” African Journal of Biotechnology, vol. 5(5), pp. 472-476,
2005.
[12] H. Pourrat, F. Regerat, A. Pourrat, D. Jean, “Production of gallic acid
from tara tannin by a strain of A. niger,” J. Ferment. Technol., vol.63,
pp. 401-403, 1985.
[13] K. Schuegerl, H Bellgardt Bioreaction engineering, modeling and
control. Springer, Berlin Heidelberg New York. 2000.
[14] A. P. M. Tavares, M. A. Z. Coelho, J. A. P. Cautinho, A. M. R. B.
Xavier, “Laccase improvement in submerged cultivation: induced
production and kinetic modeling,” J Chem Technol Biotechnol, vol. 80,
pp. 669- 676, 2005.
[15] M Thilakavathi, T Basak, T Panda, “Modeling of enzyme production
kinetics,” Appl Microbiol Biotechnol, vol.73, pp.991-1007, 2007.
[16] R. M. Weiss, D F. Ollis, “Extracellular microbial polysaccharides:
Substrate, biomass and Product kinetic equations for batch xanthan gum
fermentation,” Biotechnol. Bioengg, vol.22, pp.859-873.
[1] C. N Aguilar, and G. Gutiérrez-Sánchez, “Review: sources, properties,
applications and potential uses of tannin acyl hydrolase,” Food Sci.
Technol. Int, vol.7, pp. 373-382, 2001.
[2] A. A Esener, J. A Roels, N. W Kossen,”Theory and applications of
unstructured growth models: Kinetic and energetic aspects,” Biotechnol
Bioeng, vol. 25, pp.2803-2841, 1983.
[3] A.E Ghaly, M. Kamal, L. R. Correia,”Kinetic modeling of continuous
submerged fermentation of cheese whey for single cell protein
production,” Bioresource Technology, vol. 96, pp. 1143-1152, 2005.
[4] A E. Haggerman, L G. Butler. “Protein precipitation method for
determination of tannins,” J. Agric. Food Chem., vol.26, pp.809–812,
1978.
[5] E. Haslam, J E. Stangroom, “The esterase and depsidase activities of
tannase,” Biochem. J., vol.99, pp.28-31, 1966.
[6] P K. Lekha and B K. Lonsane, “Production and application of tannin
acyl hydrolase; state of the art,” Advances in Applied Microbiology, vol.
44, pp.215–260, 1997.
[7] W. Madhavakrishna, S M. Bose, Y. Nayudamma, “Estimation of tannase
and certain oxidizing enzymes in Indian vegetable tanstuffs,” Bull. Cent.
Leath. Res. Instit, vol. 7, pp. 1-11, 1960.
[8] K. C. Mondal, D. Banerjee, M. Jana and B. R. Pati, “Calorimetric assay
method for determination of the tannase activity,” Analytical
Biochemistry, vol. 295, pp. 168-171, 2001.
[9] J. Monod, “The growth of Bacterial cultures,” Annual Rev Microbiol,
vol.3, pp- 371-394, 1949.
[10] R A Moraine, P Rogovin,” Xanthan biopolymer production at increased
concentration by pH control,” Biotechnol Bioengg, vol.13, pp.381-391,
1971.
[11] G. Narasimha, A. Sridevi, B. Viswanath, M. Subhosh Chandra, B.
Rajasekarreddy, “Nutrient effects on production of cellulytic enzymes
by A. niger,” African Journal of Biotechnology, vol. 5(5), pp. 472-476,
2005.
[12] H. Pourrat, F. Regerat, A. Pourrat, D. Jean, “Production of gallic acid
from tara tannin by a strain of A. niger,” J. Ferment. Technol., vol.63,
pp. 401-403, 1985.
[13] K. Schuegerl, H Bellgardt Bioreaction engineering, modeling and
control. Springer, Berlin Heidelberg New York. 2000.
[14] A. P. M. Tavares, M. A. Z. Coelho, J. A. P. Cautinho, A. M. R. B.
Xavier, “Laccase improvement in submerged cultivation: induced
production and kinetic modeling,” J Chem Technol Biotechnol, vol. 80,
pp. 669- 676, 2005.
[15] M Thilakavathi, T Basak, T Panda, “Modeling of enzyme production
kinetics,” Appl Microbiol Biotechnol, vol.73, pp.991-1007, 2007.
[16] R. M. Weiss, D F. Ollis, “Extracellular microbial polysaccharides:
Substrate, biomass and Product kinetic equations for batch xanthan gum
fermentation,” Biotechnol. Bioengg, vol.22, pp.859-873.
@article{"International Journal of Biological, Life and Agricultural Sciences:70680", author = "S. K. Mohan and T. Viruthagiri and C. Arunkumar", title = "Kinetic Studies on Microbial Production of Tannase Using Redgram Husk", abstract = "Tannase (tannin acyl hydrolase, E.C.3.1.1.20) is an
important hydrolysable enzyme with innumerable applications and
industrial potential. In the present study, a kinetic model has been
developed for the batch fermentation used for the production of
tannase by A.flavus MTCC 3783. Maximum tannase activity of
143.30 U/ml was obtained at 96 hours under optimum operating
conditions at 35oC, an initial pH of 5.5 and with an inducer tannic
acid concentration of 3% (w/v) for a fermentation period of 120
hours. The biomass concentration reaches a maximum of 6.62 g/l at
96 hours and further there was no increase in biomass concentration
till the end of the fermentation. Various unstructured kinetic models
were analyzed to simulate the experimental values of microbial
growth, tannase activity and substrate concentration. The Logistic
model for microbial growth , Luedeking - Piret model for production
of tannase and Substrate utilization kinetic model for utilization of
substrate were capable of predicting the fermentation profile with
high coefficient of determination (R2) values of 0.980, 0.942 and
0.983 respectively. The results indicated that the unstructured models
were able to describe the fermentation kinetics more effectively.", keywords = "Aspergillus flavus, Batch fermentation, Kinetic
model, Tannase, Unstructured models.", volume = "8", number = "9", pages = "1077-4", }