Abstract: The genus Azotobacter has been widely used as bio-fertilizer due to its significant effects on the stimulation and promotion of plant growth in various agricultural species of commercial interest. In order to obtain significantly viable cellular concentration, a scale-up strategy for a liquid fermentation process (SmF) with two strains of A. chroococcum (named Ac1 and Ac10) was validated and adjusted at laboratory and pilot scale. A batch fermentation process under previously defined conditions was carried out on a biorreactor Infors®, model Minifors of 3.5 L, which served as a baseline for this research. For the purpose of increasing process efficiency, the effect of the reduction of stirring speed was evaluated in combination with a fed-batch-type fermentation laboratory scale. To reproduce the efficiency parameters obtained, a scale-up strategy with geometric and fluid dynamic behavior similarities was evaluated. According to the analysis of variance, this scale-up strategy did not have significant effect on cellular concentration and in laboratory and pilot fermentations (Tukey, p > 0.05). Regarding air consumption, fermentation process at pilot scale showed a reduction of 23% versus the baseline. The percentage of reduction related to energy consumption reduction under laboratory and pilot scale conditions was 96.9% compared with baseline.
Abstract: Tannase (tannin acyl hydrolase, E.C.3.1.1.20) is an
important hydrolysable enzyme with innumerable applications and
industrial potential. In the present study, a kinetic model has been
developed for the batch fermentation used for the production of
tannase by A.flavus MTCC 3783. Maximum tannase activity of
143.30 U/ml was obtained at 96 hours under optimum operating
conditions at 35oC, an initial pH of 5.5 and with an inducer tannic
acid concentration of 3% (w/v) for a fermentation period of 120
hours. The biomass concentration reaches a maximum of 6.62 g/l at
96 hours and further there was no increase in biomass concentration
till the end of the fermentation. Various unstructured kinetic models
were analyzed to simulate the experimental values of microbial
growth, tannase activity and substrate concentration. The Logistic
model for microbial growth , Luedeking - Piret model for production
of tannase and Substrate utilization kinetic model for utilization of
substrate were capable of predicting the fermentation profile with
high coefficient of determination (R2) values of 0.980, 0.942 and
0.983 respectively. The results indicated that the unstructured models
were able to describe the fermentation kinetics more effectively.
Abstract: A vacuum fractionation technique was introduced to remove ethanol from fermentation broth. The effect of initial glucose and ethanol concentrations were investigated for specific productivity. The inhibitory ethanol concentration was observed at 100 g/L. In order to increase the fermentation performance, the ethanol product was removed as soon as it is produced. The broth was boiled at 35oC by reducing the pressure to 65 mBar. The ethanol/water vapor was fractionated for up to 90 wt% before leaving the column. Ethanol concentration in the broth was kept lower than 25 g/L, thus minimized the product inhibition effect to the yeast cells. For batch extractive fermentation, a high substrate utilization rate was obtained at 26.6 g/L.h and most of glucose was consumed within 21 h. For repeated-batch extractive fermentation, addition of glucose was carried out up to 9 times and ethanol was produced more than 8-fold higher than batch fermentation.
Abstract: In this study, static batch fermentation was used for bacterial cellulose production in date syrup solution (Bx. 10%) at 28°C using Gluconacetobacter. xylinus (PTCC 1734). The physicochemical properties of standard Sigma CMC and the produced carboxymethyl bacterial cellulose (CMBC) were studied using FT-IR spectroscopy, X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM). According to the FT-IR spectra the bands at 1664 and 1431 cm-1 indicate that carboxylic acid groups and carboxylate groups exist on the surface. The SEM imaging of CMBC and CMC carried out in magnification of 1K. Comparing the SEM imaging obviously showed that the ribbon shape in CMC remained but the length of ribbons became shorter while that shape changed to flake shape for CMBC. Determination of the area under XRD patterns demonstrated that the crystallinity amount of CMC was more than that for CMBC (51.08% and 81.84% for CMBC and CMC, respectively).
Abstract: In this study, a mathematical model was proposed and
the accuracy of this model was assessed to predict the growth of
Pseudomonas aeruginosa and rhamnolipid production under nitrogen
limiting (sodium nitrate) fed-batch fermentation. All of the
parameters used in this model were achieved individually without
using any data from the literature.
The overall growth kinetic of the strain was evaluated using a
dual-parallel substrate Monod equation which was described by
several batch experimental data. Fed-batch data under different
glycerol (as the sole carbon source, C/N=10) concentrations and feed
flow rates were used to describe the proposed fed-batch model and
other parameters. In order to verify the accuracy of the proposed
model several verification experiments were performed in a vast
range of initial glycerol concentrations. While the results showed an
acceptable prediction for rhamnolipid production (less than 10%
error), in case of biomass prediction the errors were less than 23%. It
was also found that the rhamnolipid production by P. aeruginosa was
more sensitive at low glycerol concentrations.
Based on the findings of this work, it was concluded that the
proposed model could effectively be employed for rhamnolipid
production by this strain under fed-batch fermentation on up to 80 g l-
1 glycerol.
Abstract: Gluconic acid is one of interesting chemical products
in industries such as detergents, leather, photographic, textile, and
especially in food and pharmaceutical industries. Fermentation is an
advantageous process to produce gluconic acid. Mathematical
modeling is important in the design and operation of fermentation
process. In fact, kinetic data must be available for modeling. The
kinetic parameters of gluconic acid production by Aspergillus niger
in batch culture was studied in this research at initial substrate
concentration of 150, 200 and 250 g/l. The kinetic models used were
logistic equation for growth, Luedeking-Piret equation for gluconic
acid formation, and Luedeking-Piret-like equation for glucose
consumption. The Kinetic parameters in the model were obtained by
minimizing non linear least squares curve fitting.
Abstract: Microbial oil was produced by soil isolated
oleaginous yeast YU5/2 in flask-batch fermentation. The yeast was
identified by molecular genetics technique based on sequence
analysis of the variable D1/D2 domain of the large subunit (26S)
ribosomal DNA and it was identified as Torulaspora globosa. T.
globosa YU5/2 supported maximum values of 0.520 g/L/d, 0.472 g
lipid/g cells, 4.16 g/L, and 0.156 g/L/d for volumetric lipid
production rate, and specific yield of lipid, lipid concentration, and
specific rate of lipid production respectively, when culture was
performed in nitrogen-limiting medium supplemented with 80g/L
glucose. Among the carbon sources tested, maximum cell yield
coefficient (YX/S, g/L), maximum specific yield of lipid (YP/X, g
lipid/g cells) and volumetric lipid production rate (QP, g/L/d) were
found of 0.728, 0.237, and 0.619, respectively, using sweet potato
tubers hydrolysates as carbon source.
Abstract: Simultaneous Saccharification and Fermentation (SSF) of sugarcane bagasse by cellulase and Pachysolen tannophilus MTCC *1077 were investigated in the present study. Important process variables for ethanol production form pretreated bagasse were optimized using Response Surface Methodology (RSM) based on central composite design (CCD) experiments. A 23 five level CCD experiments with central and axial points was used to develop a statistical model for the optimization of process variables such as incubation temperature (25–45°) X1, pH (5.0–7.0) X2 and fermentation time (24–120 h) X3. Data obtained from RSM on ethanol production were subjected to the analysis of variance (ANOVA) and analyzed using a second order polynomial equation and contour plots were used to study the interactions among three relevant variables of the fermentation process. The fermentation experiments were carried out using an online monitored modular fermenter 2L capacity. The processing parameters setup for reaching a maximum response for ethanol production was obtained when applying the optimum values for temperature (32°C), pH (5.6) and fermentation time (110 h). Maximum ethanol concentration (3.36 g/l) was obtained from 50 g/l pretreated sugarcane bagasse at the optimized process conditions in aerobic batch fermentation. Kinetic models such as Monod, Modified Logistic model, Modified Logistic incorporated Leudeking – Piret model and Modified Logistic incorporated Modified Leudeking – Piret model have been evaluated and the constants were predicted.
Abstract: Bacterial cellulose, a biopolysaccharide, is produced by the bacterium, Gluconacetobacter xylinus. Static batch fermentation for bacterial cellulose production was studied in sucrose and date syrup solutions (Bx. 10%) at 28 °C using G. xylinus (PTCC, 1734). Results showed that the maximum yields of bacterial cellulose (BC) were 4.35 and 1.69 g/l00 ml for date syrup and sucrose medium after 336 hours fermentation period, respectively. Comparison of FTIR spectrum of cellulose with BC indicated appropriate coincidence which proved that the component produced by G. xylinus was cellulose. Determination of the area under X-ray diffractometry patterns demonstrated that the crystallinity amount of cellulose (83.61%) was more than that for the BC (60.73%). The scanning electron microscopy imaging of BC and cellulose were carried out in two magnifications of 1 and 6K. Results showed that the diameter ratio of BC to cellulose was approximately 1/30 which indicated more delicacy of BC fibers relative to cellulose.
Abstract: Batch fermentation of 5, 10 and 25 g/L biodiesel
derived crude glycerol was carried out at 30, 37 and 450C by
Clostridium pasteurianum cells immobilized on silica. Maximum
yield of 1,3-propanediol (PDO) (0.60 mol/mol), and ethanol (0.26
mol/mol) were obtained from 10 g/L crude glycerol at 30 and 370C
respectively. Maximum yield of butanol (0.28 mol/mol substrate
added) was obtained at 370C with 25 g/L substrate. None of the three
products were detected at 45oC even after 10 days of fermentation.
Only traces of ethanol (0.01 mol/mol) were detected at 450C with 5
g/L substrate. The results obtained for 25 g/L substrate utilization
were fitted in first order rate equation to obtain the values of rate
constant at three different temperatures for bioconversion of glycerol.
First order rate constants for bioconversion of glycerol at 30, 37 and
45oC were found to be 0.198, 0.294 and 0.029/day respectively.
Activation energy (Ea) for crude glycerol bioconversion was
calculated to be 57.62 kcal/mol.
Abstract: The objective of this work is to produce heterotrophic
microalgal lipid in flask-batch fermentation. Chlorella sp. KKU-S2
supported maximum values of 0.374 g/L/d, 0.478 g lipid/g cells, and
0.112 g/L/d for volumetric lipid production rate, and specific yield of
lipid, and specific rate of lipid production, respectively when culture
was performed on BG-11 medium supplemented with 50g/L glucose.
Among the carbon sources tested, maximum cell yield coefficient
(YX/S, g/L), maximum specific yield of lipid (YP/X, g lipid/g cells) and
volumetric lipid production rate (QP, g/L/d) were found of 0.728,
0.237, and 0.619, respectively, using sugarcane molasses as carbon
source. The main components of fatty acid from extracted lipid were
palmitic acid, stearic acid, oleic acid and linoleic acid which similar
to vegetable oils and suitable for biodiesel production.
Abstract: Ethanol has been known for a long time, being
perhaps the oldest product obtained through traditional biotechnology
fermentation. Agriculture waste as substrate in fermentation is vastly
discussed as alternative to replace edible food and utilization of
organic material. Pineapple peel, highly potential source as substrate
is a by-product of the pineapple processing industry. Bio-ethanol
from pineapple (Ananas comosus) peel extract was carried out by
controlling fermentation without any treatment. Saccharomyces
ellipsoides was used as inoculum in this fermentation process as it is
naturally found at the pineapple skin. In this study, the capability of
Response Surface Methodology (RSM) for optimization of ethanol
production from pineapple peel extract using Saccharomyces
ellipsoideus in batch fermentation process was investigated. Effect of
five test variables in a defined range of inoculum concentration 6-
14% (v/v), pH (4.0-6.0), sugar concentration (14-22°Brix),
temperature (24-32°C) and time of incubation (30-54 hrs) on the
ethanol production were evaluated. Data obtained from experiment
were analyzed with RSM of MINITAB Software (Version 15)
whereby optimum ethanol concentration of 8.637% (v/v) was
determined. The optimum condition of 14% (v/v) inoculum
concentration, pH 6, 22°Brix, 26°C and 30hours of incubation. The
significant regression equation or model at the 5% level with
correlation value of 99.96% was also obtained.
Abstract: Studies on Simultaneous Saccharification and Fermentation (SSF) of corn flour, a major agricultural product as the substrate using starch digesting glucoamylase enzyme derived from Aspergillus niger and non starch digesting and sugar fermenting Saccharomyces cerevisiae in a batch fermentation. Experiments based on Central Composite Design (CCD) were conducted to study the effect of substrate concentration, pH, temperature, enzyme concentration on Ethanol Concentration and the above parameters were optimized using Response Surface Methodology (RSM). The optimum values of substrate concentration, pH, temperature and enzyme concentration were found to be 160 g/l, 5.5, 30°C and 50 IU respectively. The effect of inoculums age on ethanol concentration was also investigated. The corn flour solution equivalent to 16% initial starch concentration gave the highest ethanol concentration of 63.04 g/l after 48 h of fermentation at optimum conditions of pH and temperature. Monod model and Logistic model were used for growth kinetics and Leudeking – Piret model was used for product formation kinetics.