In vitro Antioxidant Activity of Two Selected Herbal Medicines
Hot aqueous and methanol extracts of the two selected
herbal medicines such are Vellarugu chooranam (V.C) and
Amukkirai Chooranam (A.C) were examined for total phenolic and
flavonoid contents and in vitro antioxidant activity using four
different methods. The total phenolic and flavonoid contents in
methanol extract of V.C were found to be higher (44.41±1.26mg
GAE/g; 174.44±9.32mg QE/g) than in the methanol extract of A.C
(20.56±0.67mg GAE/g; 7.21±0.85mg QE/g). Hot methanol and
aqueous extracts of both medicines showed low antioxidant activity
in DPPH, ABTS, and FRAP methods and Iron chelating activity not
found at highest possible concentration. V.C contains higher
concentrations of total phenolic and flavonoid contents than A.C and
can also exert greater antioxidant activity than A.C, although the
activities demonstrated were lower than the positive control Trolox.
The in vitro antioxidant activity was not related with the total
phenolic and flavonoid contents of the methanol and aqueous extracts
of both herbal medicines (A.C and V.C).
[1] M. A. Ebrahimzadeh, F. Pourmorad and A. R. Bekhradnia, “Iron
chelating activity, Phenol and flavonoid content of some medicinal
plants from Iran”, African Journal of Biotechnology, vol. 7 (18), 17
September 2008, pp. 3188-3192.
[2] S. Panchawat, “In vitro Free Radical Scavenging Activity of Leaves
Extracts of Withania somnifera” Recent Research in Science and
Technology RRST-Pharmacology, vol.3 (11), 2011, pp. 40-43.
[3] N. R .Bhalodia, P. B. Nariya, R. N. Acharya, and V. J. Shukla,
“Evaluation of in vitro Antioxidant activity of Flowers of Cassia fistula
Linn.”, International Journal of PharmTech Research, vol. 3, No.1, Jan-
Mar 2011; pp 589-599.
[4] P. K. Jain and R. K. Agrawal, “Antioxidant and Free Radical
Scavenging Properties of Developed Mono - and Polyherbal
Formulations”, Asian J. Exp. Sci., vol. 22, No. 3, 2008, pp. 213-220.
[5] K. S. Murukesu muthaliyar, “Gunapadam I (Vegetable kingdom)”,
Siddha maruthuva varrium, Chennai 600106, 1936, pp. 1, 606-60.
[6] S. Kannusamipillai, “Siddha Vaidthiya Patharththa Guna Vilakam,
(Moola varkkam)”, B. Rathinanajakar & Sons, Chennai, 1967 & 1998,
pp. 6, 09-671.
[7] V. L. Singleton, R. Orthofer, and R. M. Lamuela-Raventos, “Analysis of
total phenols and other oxidation substrates and antioxidants by means
of Folin-Ciocaltue reagent”, Meth Enzymol, 299, 1999, pp. 152-178.
[8] A. V. Badarinath, K. Mallikarjuna RAo, C. M. S. Chetty, S. Ramkanth,
T. V. S. Rajan, and K. Gnanaprakash, “A Review on in vitro
Antioxidant Methods: Comparisions, Correlations and Considerations”,
International Journal of Pharm Tech Research, vol. 2. no.2, 2010, pp.
1276-85.
[9] P. Carter, “Spectrophotometric determination of serum iron at the submicrogram
level with a new reagent ferrozine”, Annual Biochemistry,
40, 1971, pp. 450-58.
[10] M. S. Blois, “Antioxidant determination by use of stable free radical”,
Nature, 181, 1958, pp. 1199-1200.
[11] I. F. F. Benzine, and Y. T. Szeto, “Total antioxidant capacity of teas by
the ferric reducing antioxidant power assay”, J. Agric. Food Chem., 47,
1999, pp. 633-36.
[12] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, and M. Yang,
“Antioxidant activity applying an improved ABTS radical cation
decolorization Assay”, Free Radical Biol. Med, 26, 1999, pp. 1231-37.
[13] I. Hinneburg, H. J. D. Dorman, and R. Hiltunen, “Antioxidant activities
of extracts from selected culinary herbs and spices”, Food Chemistry 97
2006, pp. 122–129.
[14] S. M. Nabavi, M. A. Ebrahimzadeli, S. F. Nabavi, A. Hamidina and A.
R. Bekhradnia, “Determination of antioxidant activity, phenol and
flavonoid content of Parrota Persia MEY”, Pharmacology online, 2:
2008, pp. 560-567.
[15] S. A. Yadava, L. F. Hakkim, and R. Sathishkumar, “Antioxidant activity
of Withania somnifera (L) Dunal by different solvent extraction
methods”, Journal of Pharmacy Research, vol. 4 (5), 2011, pp. 1428-
1430.
[16] M. Kessler, G.C. Ubeaud and L. Jung, “Anti- and pro-oxidant activity of
rutin and quercetin derivatives”, J. Pharm. Pharmacol. 53, 2003, pp.
131-142.
[17] A. Prakash, F. Rigelhof and E. Miller, (Dr. Jonathan DeVries, Editor),
“Antioxidant Activity”, Medallion Laboratories Analytical Progress,
www.medallionlabs.com.
[1] M. A. Ebrahimzadeh, F. Pourmorad and A. R. Bekhradnia, “Iron
chelating activity, Phenol and flavonoid content of some medicinal
plants from Iran”, African Journal of Biotechnology, vol. 7 (18), 17
September 2008, pp. 3188-3192.
[2] S. Panchawat, “In vitro Free Radical Scavenging Activity of Leaves
Extracts of Withania somnifera” Recent Research in Science and
Technology RRST-Pharmacology, vol.3 (11), 2011, pp. 40-43.
[3] N. R .Bhalodia, P. B. Nariya, R. N. Acharya, and V. J. Shukla,
“Evaluation of in vitro Antioxidant activity of Flowers of Cassia fistula
Linn.”, International Journal of PharmTech Research, vol. 3, No.1, Jan-
Mar 2011; pp 589-599.
[4] P. K. Jain and R. K. Agrawal, “Antioxidant and Free Radical
Scavenging Properties of Developed Mono - and Polyherbal
Formulations”, Asian J. Exp. Sci., vol. 22, No. 3, 2008, pp. 213-220.
[5] K. S. Murukesu muthaliyar, “Gunapadam I (Vegetable kingdom)”,
Siddha maruthuva varrium, Chennai 600106, 1936, pp. 1, 606-60.
[6] S. Kannusamipillai, “Siddha Vaidthiya Patharththa Guna Vilakam,
(Moola varkkam)”, B. Rathinanajakar & Sons, Chennai, 1967 & 1998,
pp. 6, 09-671.
[7] V. L. Singleton, R. Orthofer, and R. M. Lamuela-Raventos, “Analysis of
total phenols and other oxidation substrates and antioxidants by means
of Folin-Ciocaltue reagent”, Meth Enzymol, 299, 1999, pp. 152-178.
[8] A. V. Badarinath, K. Mallikarjuna RAo, C. M. S. Chetty, S. Ramkanth,
T. V. S. Rajan, and K. Gnanaprakash, “A Review on in vitro
Antioxidant Methods: Comparisions, Correlations and Considerations”,
International Journal of Pharm Tech Research, vol. 2. no.2, 2010, pp.
1276-85.
[9] P. Carter, “Spectrophotometric determination of serum iron at the submicrogram
level with a new reagent ferrozine”, Annual Biochemistry,
40, 1971, pp. 450-58.
[10] M. S. Blois, “Antioxidant determination by use of stable free radical”,
Nature, 181, 1958, pp. 1199-1200.
[11] I. F. F. Benzine, and Y. T. Szeto, “Total antioxidant capacity of teas by
the ferric reducing antioxidant power assay”, J. Agric. Food Chem., 47,
1999, pp. 633-36.
[12] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, and M. Yang,
“Antioxidant activity applying an improved ABTS radical cation
decolorization Assay”, Free Radical Biol. Med, 26, 1999, pp. 1231-37.
[13] I. Hinneburg, H. J. D. Dorman, and R. Hiltunen, “Antioxidant activities
of extracts from selected culinary herbs and spices”, Food Chemistry 97
2006, pp. 122–129.
[14] S. M. Nabavi, M. A. Ebrahimzadeli, S. F. Nabavi, A. Hamidina and A.
R. Bekhradnia, “Determination of antioxidant activity, phenol and
flavonoid content of Parrota Persia MEY”, Pharmacology online, 2:
2008, pp. 560-567.
[15] S. A. Yadava, L. F. Hakkim, and R. Sathishkumar, “Antioxidant activity
of Withania somnifera (L) Dunal by different solvent extraction
methods”, Journal of Pharmacy Research, vol. 4 (5), 2011, pp. 1428-
1430.
[16] M. Kessler, G.C. Ubeaud and L. Jung, “Anti- and pro-oxidant activity of
rutin and quercetin derivatives”, J. Pharm. Pharmacol. 53, 2003, pp.
131-142.
[17] A. Prakash, F. Rigelhof and E. Miller, (Dr. Jonathan DeVries, Editor),
“Antioxidant Activity”, Medallion Laboratories Analytical Progress,
www.medallionlabs.com.
@article{"International Journal of Medical, Medicine and Health Sciences:68335", author = "S. Vinotha and I. Thabrew and S. Sri Ranjani", title = "In vitro Antioxidant Activity of Two Selected Herbal Medicines", abstract = "Hot aqueous and methanol extracts of the two selected
herbal medicines such are Vellarugu chooranam (V.C) and
Amukkirai Chooranam (A.C) were examined for total phenolic and
flavonoid contents and in vitro antioxidant activity using four
different methods. The total phenolic and flavonoid contents in
methanol extract of V.C were found to be higher (44.41±1.26mg
GAE/g; 174.44±9.32mg QE/g) than in the methanol extract of A.C
(20.56±0.67mg GAE/g; 7.21±0.85mg QE/g). Hot methanol and
aqueous extracts of both medicines showed low antioxidant activity
in DPPH, ABTS, and FRAP methods and Iron chelating activity not
found at highest possible concentration. V.C contains higher
concentrations of total phenolic and flavonoid contents than A.C and
can also exert greater antioxidant activity than A.C, although the
activities demonstrated were lower than the positive control Trolox.
The in vitro antioxidant activity was not related with the total
phenolic and flavonoid contents of the methanol and aqueous extracts
of both herbal medicines (A.C and V.C).
", keywords = "Activity, Different extracts, Herbal medicine, in
vitro antioxidant.", volume = "8", number = "12", pages = "833-6", }
