Fermentative Production of Dextran using Food Industry Wastes
Dextran is a D-glucose polymer which is produced by
Leuconostoc mesenteroides grown in a sucrose-rich media. The
organism was obtained from the Persian Type Culture Collection
(PTCC) and was transferred in MRS broth medium at 30°C and pH
6.8 for 24 h. After preparation of inoculums, organisms were
inoculated into five liquid fermentation media containing either
molasses or cheese whey or different combinations of cheese whey
and molasses. After certain fermentation period, the produced
dextran was separated and dried. Dextran yield was calculated and
significant differences in different media were observed.
Furthermore, FT-IR analysis was performed and the results showed
that there were no significant differences in the produced dextran
structures.
[1] A. Tecante, A. Lopez-Munguia, and A. Garcia-Rejon, "Rheological
characterization of dextran-enzymatic synthesis media," J. Appl.
Polym.Sci., vol. 31, pp. 2337-2350, 1986.
[2] D. Kim, J. F. Robyt, S. Y. Lee, J. H. Lee, and Y. M. Kim, "Dextran
molecular size and degree of branching as a function of sucrose
concentration, pH, and temperature of reaction of Leuconostoc
mesenteroides B-512FMCM dextransucrase, " . Carbohydrate Research,
vol. 338, pp. 1183-1189, 2003.
[3] H. J. Koepsell, and H. M. Tsuchiya, "Enzymatic synthesis of dextran," J.
Bacteriol., vol. 63, pp. 293-295, 1952.
[4] M. Dols, M. Remaud-Simeon, R. Willemot, M. Vignon, and P. Monsan,
"Structural characterisation of the maltose acceptor-products synthesized
by Leuconostoc mesenteroides NRRL B1299 dextransucrase,"
Carbohydr. Res., vol. 305, pp. 549-559, 1998.
[5] M. Santos, A. Rodriguesb, and J. A. Teixeira, "Production of dextran
and fructose from carob pod extract and cheese whey by Leuconostoc
mesenteroides NRRL B512 (f)," Biochemical Engineering J., vol. 25,
pp. 1-6, 2005.
[6] M. Santos, J. Teixeira, and A. Rodrigues, "Production of dextransucrase,
dextran and fructose from sucrose using Leuconostoc mesenteroides
NRRL B512 (f)," Biochemical Engineering J., vol. 4, pp. 177-188,
2000.
[7] S. M. Holt, H. Al-Sheikh, and K. J. Shin, "Characterization of dextranproducing
Leuconostoc strains," Letters in Applied Microbiology, vol.
32, pp. 185-189, 2001.
[8] V. B. Veljkovic, M. L. Lazic, D. J. Rutic, S.M. Jovanovic, and D.U.
Skala, "Effects of aeration on extracellular dextransucrase production by
Leuconostoc mesenteroides," Enzyme Microb. Technol., vol. 14, pp.
665-668, 1992.
[1] A. Tecante, A. Lopez-Munguia, and A. Garcia-Rejon, "Rheological
characterization of dextran-enzymatic synthesis media," J. Appl.
Polym.Sci., vol. 31, pp. 2337-2350, 1986.
[2] D. Kim, J. F. Robyt, S. Y. Lee, J. H. Lee, and Y. M. Kim, "Dextran
molecular size and degree of branching as a function of sucrose
concentration, pH, and temperature of reaction of Leuconostoc
mesenteroides B-512FMCM dextransucrase, " . Carbohydrate Research,
vol. 338, pp. 1183-1189, 2003.
[3] H. J. Koepsell, and H. M. Tsuchiya, "Enzymatic synthesis of dextran," J.
Bacteriol., vol. 63, pp. 293-295, 1952.
[4] M. Dols, M. Remaud-Simeon, R. Willemot, M. Vignon, and P. Monsan,
"Structural characterisation of the maltose acceptor-products synthesized
by Leuconostoc mesenteroides NRRL B1299 dextransucrase,"
Carbohydr. Res., vol. 305, pp. 549-559, 1998.
[5] M. Santos, A. Rodriguesb, and J. A. Teixeira, "Production of dextran
and fructose from carob pod extract and cheese whey by Leuconostoc
mesenteroides NRRL B512 (f)," Biochemical Engineering J., vol. 25,
pp. 1-6, 2005.
[6] M. Santos, J. Teixeira, and A. Rodrigues, "Production of dextransucrase,
dextran and fructose from sucrose using Leuconostoc mesenteroides
NRRL B512 (f)," Biochemical Engineering J., vol. 4, pp. 177-188,
2000.
[7] S. M. Holt, H. Al-Sheikh, and K. J. Shin, "Characterization of dextranproducing
Leuconostoc strains," Letters in Applied Microbiology, vol.
32, pp. 185-189, 2001.
[8] V. B. Veljkovic, M. L. Lazic, D. J. Rutic, S.M. Jovanovic, and D.U.
Skala, "Effects of aeration on extracellular dextransucrase production by
Leuconostoc mesenteroides," Enzyme Microb. Technol., vol. 14, pp.
665-668, 1992.
@article{"International Journal of Business, Human and Social Sciences:56951", author = "Marzieh Moosavi-Nasab and Mohsen Gavahian and Ali R. Yousefi and Hamed Askari", title = "Fermentative Production of Dextran using Food Industry Wastes", abstract = "Dextran is a D-glucose polymer which is produced by
Leuconostoc mesenteroides grown in a sucrose-rich media. The
organism was obtained from the Persian Type Culture Collection
(PTCC) and was transferred in MRS broth medium at 30°C and pH
6.8 for 24 h. After preparation of inoculums, organisms were
inoculated into five liquid fermentation media containing either
molasses or cheese whey or different combinations of cheese whey
and molasses. After certain fermentation period, the produced
dextran was separated and dried. Dextran yield was calculated and
significant differences in different media were observed.
Furthermore, FT-IR analysis was performed and the results showed
that there were no significant differences in the produced dextran
structures.", keywords = "Dextran, Leuconostoc mesenteroides, Molasses,Whey", volume = "4", number = "8", pages = "1897-3", }