Determination of Cyclic Citrullinated Peptide Antibodies on Quartz Crystal Microbalance Based Nanosensors
In this study, we have focused our attention on
combining of molecular imprinting into nanofilms and QCM
nanosensor approaches and producing QCM nanosensor for anti-
CCP, chosen as model protein, using anti-CCP imprinted nanofilms.
The nonimprinted nanosensor was also prepared to evaluate the
selectivity of the imprinted nanosensor. Anti-CCP imprinted QCM
nanosensor was tested for real time detection of anti-CCP from
aqueous solution. The kinetic and affinity studies were determined by
using anti-CCP solutions with different concentrations. The
responses related with mass shifts (%m) and frequency shifts (%f)
were used to evaluate adsorption properties. To show the selectivity
of the anti-CCP imprinted QCM nanosensor, competitive adsorption
of anti-CCP and IgM was investigated. The results indicate that anti-
CCP imprinted QCM nanosensor has higher adsorption capabilities
for anti-CCP than for IgM, due to selective cavities in the polymer
structure.
[1] E. Yılmaz, L. Uzun, A.Y. Rad, U. Kalyoncu, S. Ünal, A. Denizli,
“Specific adsorption of the autoantibodies from rheumatoid arthritis
patient plasma using histidine-containing affinity beads,” J. Biomat. Sci-
Polym E, vol. 19, pp. 875-892, 2008.
[2] M.A. Van Boekel, E.R. Vossenaar, F.H. van den Hoogen, W.J. van
Venroo, “Autoantibody systems in rheumatoid arthritis: specificity,
sensitivity and diagnostic value,” Arthritis Res, vol. 4, pp. 87-93, 2002.
[3] F. J. Diaz, A. Rojas-Villarraga, J. C. Salazar, A. Iglesias-Gamarra, R. D.
Mantilla, J.M. Anaya, “Anti-CCP antibodies are associated with early
age at onset in patients with rheumatoid arthritis,” Joint Bone Spine, vol.
78, pp. 175-178, 2011.
[4] J.L. Caro-Oleas, A. Fernández-Suárez, S. Reneses Cesteros, C. Porrino,
A. Núñez-Roldán, I. Wichmann Schlipf, “Evaluation of third generation
anti-CCP antibodies in the diagnosis of rheumatoid arthritis from
undifferentiated polyarthritis after 4 years of follow-up,” Clin Exp
Rheumatol, vol.26, pp. 461-463, 2008.
[5] N. Bereli, Y. Saylan, L. Uzun, R. Say, A. Denizli, “L-Histidine
imprinted supermacroporous cryogels for protein recognition,” Sep Purif
Technol, vol. 82, pp. 28-35, 2011.
[6] Y. Saylan, R. Üzek, L. Uzun, A. Denizli, “Surface imprinting approach
for preparing specific adsorbent for IgG separation,” J. Biomat. Sci-
Polym E, vol. 25, pp. 881-894, 2014.
[7] Y. Lv, T. Tan, F. Svec, “Molecular imprinting of proteins in polymers
attached to the surface of nanomaterials for selective recognition of
biomacromolecules,” Biotechnol. Adv, vol. 31, pp. 1172-1186, 2013.
[8] G. Sener, E. Özgur, E. Yılmaz, L. Uzun, R. Say, A. Denizli, “Quartz
crystal microbalance based nanosensor for lysozyme detection with
lysozyme imprinted nanoparticles,” Biosens Bioelectron, vol. 26, pp.
815-821, 2010.
[9] E. Yilmaz, D. Majidi, E. Ozgur, A. Denizli, “Whole cell imprinting
based Escherichia coli sensors: A study for SPR and QCM,” Sensors
Actuator, vol. 209, pp. 714-721, 2015.
[10] A. H. Wu, M. J. Syu, “Synthesis of bilirubin imprinted polymer thin film
for the continuous detection of bilirubin in an MIP/QCM/FIA system,”
Biosens Bioelectron, vol. 21, pp. 2345-2353, 2006.
[11] S. Emir Diltemiz, D. Hür, A. Ersöz, A. Denizli, R. Say, “Designing of
MIP based QCM sensor having thymine recognition sites based on
biomimicking DNA approach,” Biosensor Bioelectron, vol. 25 pp. 599-
603, 2009.
[12] K. Feng, J. Li, J. H.Jiang, G. L. Shen, R. Q. Yu, “QCM detection of
DNA targets with single-base mutation based on DNA ligase reaction
and biocatalyzed deposition amplification,” Biosens Bioelectron, vol. 22
pp. 1651-1657, 2007. [13] A. Ersöz, S. Emir Diltemiz , A. Atılır Özcan, A. Denizli, R. Say, “8-
OHdG sensing with MIP based solid phase extraction and QCM
technique,” Sensors Actuator, vol. 137, pp. 7-11, 2009.
[14] I. Mannelli, M. Minunni, S. Tombelli, M. Mascini, “Quartz crystal
microbalance (QCM) affinity biosensor for genetically modified
organisms (GMOs) detection,” Biosensor Bioelectron, vol. 18 pp. 129-
140, 2003.
[1] E. Yılmaz, L. Uzun, A.Y. Rad, U. Kalyoncu, S. Ünal, A. Denizli,
“Specific adsorption of the autoantibodies from rheumatoid arthritis
patient plasma using histidine-containing affinity beads,” J. Biomat. Sci-
Polym E, vol. 19, pp. 875-892, 2008.
[2] M.A. Van Boekel, E.R. Vossenaar, F.H. van den Hoogen, W.J. van
Venroo, “Autoantibody systems in rheumatoid arthritis: specificity,
sensitivity and diagnostic value,” Arthritis Res, vol. 4, pp. 87-93, 2002.
[3] F. J. Diaz, A. Rojas-Villarraga, J. C. Salazar, A. Iglesias-Gamarra, R. D.
Mantilla, J.M. Anaya, “Anti-CCP antibodies are associated with early
age at onset in patients with rheumatoid arthritis,” Joint Bone Spine, vol.
78, pp. 175-178, 2011.
[4] J.L. Caro-Oleas, A. Fernández-Suárez, S. Reneses Cesteros, C. Porrino,
A. Núñez-Roldán, I. Wichmann Schlipf, “Evaluation of third generation
anti-CCP antibodies in the diagnosis of rheumatoid arthritis from
undifferentiated polyarthritis after 4 years of follow-up,” Clin Exp
Rheumatol, vol.26, pp. 461-463, 2008.
[5] N. Bereli, Y. Saylan, L. Uzun, R. Say, A. Denizli, “L-Histidine
imprinted supermacroporous cryogels for protein recognition,” Sep Purif
Technol, vol. 82, pp. 28-35, 2011.
[6] Y. Saylan, R. Üzek, L. Uzun, A. Denizli, “Surface imprinting approach
for preparing specific adsorbent for IgG separation,” J. Biomat. Sci-
Polym E, vol. 25, pp. 881-894, 2014.
[7] Y. Lv, T. Tan, F. Svec, “Molecular imprinting of proteins in polymers
attached to the surface of nanomaterials for selective recognition of
biomacromolecules,” Biotechnol. Adv, vol. 31, pp. 1172-1186, 2013.
[8] G. Sener, E. Özgur, E. Yılmaz, L. Uzun, R. Say, A. Denizli, “Quartz
crystal microbalance based nanosensor for lysozyme detection with
lysozyme imprinted nanoparticles,” Biosens Bioelectron, vol. 26, pp.
815-821, 2010.
[9] E. Yilmaz, D. Majidi, E. Ozgur, A. Denizli, “Whole cell imprinting
based Escherichia coli sensors: A study for SPR and QCM,” Sensors
Actuator, vol. 209, pp. 714-721, 2015.
[10] A. H. Wu, M. J. Syu, “Synthesis of bilirubin imprinted polymer thin film
for the continuous detection of bilirubin in an MIP/QCM/FIA system,”
Biosens Bioelectron, vol. 21, pp. 2345-2353, 2006.
[11] S. Emir Diltemiz, D. Hür, A. Ersöz, A. Denizli, R. Say, “Designing of
MIP based QCM sensor having thymine recognition sites based on
biomimicking DNA approach,” Biosensor Bioelectron, vol. 25 pp. 599-
603, 2009.
[12] K. Feng, J. Li, J. H.Jiang, G. L. Shen, R. Q. Yu, “QCM detection of
DNA targets with single-base mutation based on DNA ligase reaction
and biocatalyzed deposition amplification,” Biosens Bioelectron, vol. 22
pp. 1651-1657, 2007. [13] A. Ersöz, S. Emir Diltemiz , A. Atılır Özcan, A. Denizli, R. Say, “8-
OHdG sensing with MIP based solid phase extraction and QCM
technique,” Sensors Actuator, vol. 137, pp. 7-11, 2009.
[14] I. Mannelli, M. Minunni, S. Tombelli, M. Mascini, “Quartz crystal
microbalance (QCM) affinity biosensor for genetically modified
organisms (GMOs) detection,” Biosensor Bioelectron, vol. 18 pp. 129-
140, 2003.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:69227", author = "Y. Saylan and F. Yılmaz and A. Denizli", title = "Determination of Cyclic Citrullinated Peptide Antibodies on Quartz Crystal Microbalance Based Nanosensors", abstract = "In this study, we have focused our attention on
combining of molecular imprinting into nanofilms and QCM
nanosensor approaches and producing QCM nanosensor for anti-
CCP, chosen as model protein, using anti-CCP imprinted nanofilms.
The nonimprinted nanosensor was also prepared to evaluate the
selectivity of the imprinted nanosensor. Anti-CCP imprinted QCM
nanosensor was tested for real time detection of anti-CCP from
aqueous solution. The kinetic and affinity studies were determined by
using anti-CCP solutions with different concentrations. The
responses related with mass shifts (%m) and frequency shifts (%f)
were used to evaluate adsorption properties. To show the selectivity
of the anti-CCP imprinted QCM nanosensor, competitive adsorption
of anti-CCP and IgM was investigated. The results indicate that anti-
CCP imprinted QCM nanosensor has higher adsorption capabilities
for anti-CCP than for IgM, due to selective cavities in the polymer
structure.
", keywords = "Anti-CCP, molecular imprinting, QCM nanosensor,
rheumatoid arthritis.", volume = "9", number = "3", pages = "416-4", }
