Antinociceptive and Anti-inflammatory Effects of Hydroalcohol Extract of Vitex agnus castus Fruit

In present study the effects of anti-inflammatory and antinociceptive of vitex hydro-alcoholic extract were evaluated on male mice. In inflammatory test mice were divided into 7 groups: first group was control. The second group, positive control group, received dexamethasone (15 mg/kg) and the other five groups received different doses of hydroalcohol extract of Vitex fruit (265, 365, 465, 565, and 665 mg/kg). The inflammation was caused by xylene-induced ear edema. Formalin test was used for evaluation of antinociceptive effect of extract. In this test, mice were divided into 7 groups: control, morphine (10mg/kg) as positive control group, and Vitex extract groups ((265, 365, 465, 565, and 665 mg/kg). All drugs were administered intrapritoneally, 30 min before each test. The data were analyzed using one-way ANOVA followed by Tukey-kramer multiple comparison test. Results have shown significant antiinflammatory effects of extract at all dosed as compared with control (P<0.05). In the first phase of formalin test (0-5 min) none doses of extract could inhibit acute pain, but in the second phase (15-30 min) chronic pain decreased at 265, 365, 465, and 565 mg/kg doses (P<0.05). The results of this research indicated that Vitex extract remarkably inhibited inflammation and second phase of nociception (inflammatory pain) and can be used for treatment of inflammatory diseases.

Authors:

• M. Ramezani
• Gh. Amin
• E. Jalili

Keywords:

• Anti-inflammatory
• Antinociceptive
• Mice
• Vitexagnus castus.

References:

[1] A. Zargari, Medical plants. Tehran: Tehran Univ Pub: 1996, pp. 716-718.
[2] B. Roemheld-Hamm, "Chasteberry", Am Fam Physician. , vol 72, no 5,
pp. 821-824, 2005.
[3] C. Daniele, J .Thompson Coon, MH. Pittler and E. Ernst, "Vitex agnus
castus :a systematic review of adverse events", Drug Saf ., vol 28, no 4,
pp. 319-332, 2005.
[4] JJ. Dugoua, D. Seely, D. perri, G. Koren and E. Mills, "Safety and
efficacy of chastetree (Vitex agnus castus) during pregnancy and
lactation", Can J Pharmacol., vol 15, no 1, pp. 74-79, january 2008.
[5] K. Ohyama, "Cytotoxicity and apoptotic inducibility of Vitex agnuscastus
fruit extract in cultured human normal and cancer cells and effect
on growth", Biol Pharm Bull., vol 26, no 1, pp. 10-18, Oct. 2003.
[6] S. Arokiyaraj, K. perinbam, P. Agastian and RM. Kumar, "Phytochemical
analysis and antibacterial activity of Vitex agnus castus", Int J Green
Pharm., vol 3, pp. 162-164, Aug. 2009.
[7] M. Azarnia, S. Ejtemaei-Mehr, A. Shakoor and A. Ansari, "Effects of
Vitex agnus castus on mice fetus development", Acta Medica Iranica,
vol 45, no 4:pp 263-270, Feb. 2007.
[8] MG. Dharmasiri, JR. Javakody, G. Galhena, SS. Livanage and WD.
Ratnasoorya. "Anti-,inflammatory and analgesic activities of mature
fresh leaves of Vitex negundo", J Ethnopharm., vol 87, pp. 199-206,
Apr. 2003.
[9] Y. Hu, HL. xin, QY. Zhang, HC. Zheng, K. Rahman and LP. Qin, "Antinociceptive
and anti-hyperprolactinemia activities of fructus victies and
its effective fractions and chemical constituents". Phytomedicine., vol
14, no 10, pp. 668-674, Mar. 2007.
[10] E. Nyiligira, AM. Viljoen, FR. Van Heerden, RL. Van Zyl, S F. Van
Vuuren, and PA. Steenkamp, "Phytochemistry and in vitro
pharmacological activities of South African Vitex (Verbenaceae)
species". Ethnopharm., vol 119, no 3, pp. 680-685, July. 2008.
[11] S. Nasri and S. Ebrahimi, " Medical effect of Vitex agnus- castus".
Journal of Babol University of medical Science., vol 7, pp. 49-53, 2006.
[12] S, Nasri, SH. Oryan, AH. Rohani and GHR. Amin, "The effect of Vitex
agnus castus extract on LH and testostrone in male mice", Pakistan J
of Biol Sci., vol 10, no14, pp. 2300-2307, 2007.
[13] AH. Atta and A. Alkofahi, "Anti-nociceptive and anti-inflammatory
effects of some Jordanian medical plant extracts". J of Ethnopharm., vol
60,pp. 117-124, Nov. 1998.
[14] S. Hunskaar and K. Hole, "The formalin test in mice:dissociation
between inflammatory and non-inflammatory pain", Pain, vol 30, pp.
103-114, 1987.
[15] M. Ramezani, S. Nasri and N. Yasa, "Antinociceptive and antiinflammatory
effects of isolated fractions from Apium graveolens seeds
in mice". Pharmaceutical Biol, vol 47, no 8, pp. 740-743, Oct. 2009.
[16] A. Tjolsen, DG. Berge, S. Hunskaar, JH. Rosland and K. Hole, "The
formalin test: an evaluation of the method". Pain, vol 51, pp. 5-17,
June. 1992.
[17] D. Cossuta, E. Simndi, J. Vagi, A. Hohmann and I. Prech, "Supercritical
fluid extraction of Vitex agnus castus fruit", J of Supercritical Fluids.,
Vol 47, pp. 188-194, July. 2008.
[18] J.R. Kuiatc, P.H. Amvam Zollo, G. Lamaty, C. Mentu, j.M. Bessciere,
"Composition of essential oils from the leaves of two varieties of Aspilia
ofricana (pers) Cd Adams from Cameroon". Journal of Flavor and
fragrance, vol 14, pp. 167-169, 1999.
[19] D. Falcao, S. Gomes, N. Aquino, N. Teixeira, M. Arcanjo, D.
Vasconcelos and et al. "Antinociceptive effect of the monoterpene R-
(+)-limonene in mice". Biol Pharm Bull, vol 30, no 7, pp. 1217- 1220,
July. 2007.
[20] S. Golshani, F. Karamkhani, HR. Monsef-Esfehani and I. Abdollah
Mohammad, "Antinociceptive effects of the essential oil of
Dracocephalum kotschyi in the mouse writhing test". J Pharm
Pharmaceut Sci, vol 7, no 1, pp. 76-79, Apr. 2004.
[21] S. Lin, H. Zhang, T. Han, JZ Wu, K. Rahman and et al. "In vivo effect of
casticin on acute inflammation". Zhang Xi Yi Jie He Xue Bao., vol 5, no
5, pp. 573-576, Sep. 2007.
[22] MG. Dharmasiri, JR. Javakody, G. Galhena, SS. Livanage and WD.
Ratnasoorya, "Anti-inflammatory and analgesic activities of mature
fresh leaves of Vitex negundo", J Ethnopharm., vol 87, no 2-3, pp. 199-
206, Aug. 2003.
[23] MI. Choudhary, Azizuddin, S. Jalil, SA. Nawaz, Km. Khan, RB. Tareen
and Rahman. Atta-ur, "Anti-inflammatory and lipoxygenase inhibitory
compounds from Vitex agnus-castus". Phytoter Res., vol23, no 9, pp.
1336-1339, sep. 2009.