Viability of Bradyrhizobium japanicum on Soybean Seeds Enhanced by Magnetite Nanoparticles during Desiccation
The aim of this study was to investigate whether
magnetite nanoparticles affect the viability of Bradyrhizobium
japanicum cells residing on the surface of soybean seeds during
desiccation. Different concentrations of nanoparticles suspended in
liquid medium, mixed with and adhering to Bradyrhizobium
japanicum, were investigated at two temperatures, using both
soybean seeds and glass beads as surrogates. Statistical design was a
complete randomized block (CRB) in a factorial 6×2×2×6
experimental arrangement with four replications. The most important
variable was the viability of Bradyrhizobium on the surface of the
seeds. The nanoparticles increased Bradyrhizobium viability and
inoculated seeds stored at low temperature had greater viability when
nanoparticles had been added. At the optimum nanoparticle
concentration, 50% bacterium viability on the seeds was retained
after 5 days at 4ºC. Possible explanations for the observed effects are
proposed.
[1] L. Beney, Simonin H., Mille, Y. 2007. Membrane physical stat as key
parameter for the resistance of the Gram-negative Bradyrhizobium
japanicum to hyperosmotic treatments. Arch. Microbiol. 187: 387-396.
[2] L. Beney, , Gervais P. 2001. Influence of the fluidity of the membrane
on the response of microorganisms to environmental stresses. Appl.
Microbiol. Biotechnol. 57: 34-42.
[3] M. Boumahdi, , Mary, P., Hornez, J-P. 2001. Changes in fatty acid
composition and degree of unsaturation of (brady) rhizobia as a response
to phases of growth, reduced water activities and mild desiccation.
Antonie van Leeuwenhoek. 79: 73-79.
[4] S. Cliquet, , Catroux, G. 1994. Influence of culture medium and growth
stage on the survival of Bradyrhizobium japanicum during desiccation
and storage at two relative humidities. Symbiosis. 16: 279-287.
[5] T. J. Denich, Beaudette, L.A., Lee, H., Trevors, J. T. 2003. Effects of
selected environmental and physico-chemical factors on bacterial
cytoplasmic membranes. J. Microbiol. Methods. 52: 149-182.
[6] A. D. Elbein, , Pan, Y. T., Pastuszak, I, Carroll, D. 2003. New insights
on trehalose: a multifunctional molecule. J. Glycobiol. 13: 17R-27R.
[7] M. R. Ghalamboran, Ramsden, J. J. 2009. Growth rate enhancement of
Bradyrhizobium japanicum due to magnetite nanoparticles. J.
Bionanosci. 3: 1-6.
[8] N. E. Ghittoni, Bueno, M. A. 1995. Peanut rhizobia under salt stress:
role of trehalose accumulation in strain ATCC 51466. Can. J. Microbiol.
41: 1021-1030.
[9] C. Laroche, Gervais, P. 2003. Achievement of rapid osomotic
dehydration at specific temperature could maintain high Saccharomyces
cerevisiae viability. Appl. Microbiol. Biotechnol. 60:743-747.
[10] S. B. Leslie, Israeli, E., Lighthart, B., Crowe, J. H. 1995. Trehalose and
sucrose protect bouth membranes and proteins in intact bacteria during
drying. Appl. Environ. Microbiol. 61: 3592-3597.
[11] P. Marry, Dupuy, N., Dolhem-Biremon, C., Defives, C., Tailliez, R.
1994. Differences among rhizobium meliloti and Bradyrhizobium
japanicum strains in tolerance to desiccation and storage at different
relative humidities. Soil Biol. Biochem. 26: 1125-1132.
[12] H. J. Mclntyre, Davies, H., Hore, T. A., Miller, S. H., Dufour, J. P.,
Ronson, C. W. 2007. Trehalose biosynthesis in rhizobium
leguminosarum bv. Trifolii and its role in desiccation tolerance. Appl.
Environ. Microbiol. 73: 3984-3992.
[13] Y. Mille, Beney, L., Gervais, P. 2002. Viability of Escherchia coli after
combined osmotic and thermal treatment: a plasma membrane
implication. Biochim. Biophys. Acta1567. 41-48.
[14] J. Mugnier, Jung, G. 1985. Survival of bacteria and fungi in relation to
water activity and the solvent properties of water in biopolymer gels.
Appl. Environ. Microbiol. 50: 1453-1458.
[15] R. J. Roughley, Gemell, L. G., Thompson, J. A., Brockwell, J. 1993. The
number of Bradyrhizobium sp.(Lupinus) applied to seed and its effect on
rhizosphere colonization, nodulation and yield of lupin. Soil Biol.
Biochem. 25: 1453-1458.
[16] M. P. Salema, Parker, C. A., Kiby, D. K. 1982. Death of rhizobia on
inoculated seed. Soil Biol. Biochem. 14: 13-14.
[17] P. Somasegaran, Hoben, H.J. 1994. Handbook for Rhizobia. New York:
Springer-Verlag.
[18] J. G. Streeter, 2003. Effect of trehalose on survival of Bradyrhizobium
japanicum during desiccation. J. Appl. Microbiol. 95: 484-491.
[19] J. G. Streeter, 2007. Factors affecting the survival of Bradyrhizobium
applied in liquid cultures to soya bean seeds. J. Appl. Microbiol. 103:
1282-1290.
[20] E. Tomb├ácz, Hajd├║, A., Illés. Water in contact with magnetite
nanoparticles, as seen from experiments and computer simulations. J.
Langmuir Articles., in press.
[21] J. A. C. Veriezen, De Bruijn, F., N├╝sslein, K. 2007. Responses of
rhizobia to desiccation in relation to osmotic stress, oxygen, and
temperature. J. Appl. Environ. Microbiol. 73: 3451-3459.
[22] A. G. Zavaglia, Tymczyszyn, E., De Antoni, G., Disalvo, E. A. 2003.
Action of trehalose on the preservation of Lactobacillus delbrueckii ssp.
bulgaricus by heat and osmotic dehydration. J. Appl. Microbiol. 95:
1315-1320.
[1] L. Beney, Simonin H., Mille, Y. 2007. Membrane physical stat as key
parameter for the resistance of the Gram-negative Bradyrhizobium
japanicum to hyperosmotic treatments. Arch. Microbiol. 187: 387-396.
[2] L. Beney, , Gervais P. 2001. Influence of the fluidity of the membrane
on the response of microorganisms to environmental stresses. Appl.
Microbiol. Biotechnol. 57: 34-42.
[3] M. Boumahdi, , Mary, P., Hornez, J-P. 2001. Changes in fatty acid
composition and degree of unsaturation of (brady) rhizobia as a response
to phases of growth, reduced water activities and mild desiccation.
Antonie van Leeuwenhoek. 79: 73-79.
[4] S. Cliquet, , Catroux, G. 1994. Influence of culture medium and growth
stage on the survival of Bradyrhizobium japanicum during desiccation
and storage at two relative humidities. Symbiosis. 16: 279-287.
[5] T. J. Denich, Beaudette, L.A., Lee, H., Trevors, J. T. 2003. Effects of
selected environmental and physico-chemical factors on bacterial
cytoplasmic membranes. J. Microbiol. Methods. 52: 149-182.
[6] A. D. Elbein, , Pan, Y. T., Pastuszak, I, Carroll, D. 2003. New insights
on trehalose: a multifunctional molecule. J. Glycobiol. 13: 17R-27R.
[7] M. R. Ghalamboran, Ramsden, J. J. 2009. Growth rate enhancement of
Bradyrhizobium japanicum due to magnetite nanoparticles. J.
Bionanosci. 3: 1-6.
[8] N. E. Ghittoni, Bueno, M. A. 1995. Peanut rhizobia under salt stress:
role of trehalose accumulation in strain ATCC 51466. Can. J. Microbiol.
41: 1021-1030.
[9] C. Laroche, Gervais, P. 2003. Achievement of rapid osomotic
dehydration at specific temperature could maintain high Saccharomyces
cerevisiae viability. Appl. Microbiol. Biotechnol. 60:743-747.
[10] S. B. Leslie, Israeli, E., Lighthart, B., Crowe, J. H. 1995. Trehalose and
sucrose protect bouth membranes and proteins in intact bacteria during
drying. Appl. Environ. Microbiol. 61: 3592-3597.
[11] P. Marry, Dupuy, N., Dolhem-Biremon, C., Defives, C., Tailliez, R.
1994. Differences among rhizobium meliloti and Bradyrhizobium
japanicum strains in tolerance to desiccation and storage at different
relative humidities. Soil Biol. Biochem. 26: 1125-1132.
[12] H. J. Mclntyre, Davies, H., Hore, T. A., Miller, S. H., Dufour, J. P.,
Ronson, C. W. 2007. Trehalose biosynthesis in rhizobium
leguminosarum bv. Trifolii and its role in desiccation tolerance. Appl.
Environ. Microbiol. 73: 3984-3992.
[13] Y. Mille, Beney, L., Gervais, P. 2002. Viability of Escherchia coli after
combined osmotic and thermal treatment: a plasma membrane
implication. Biochim. Biophys. Acta1567. 41-48.
[14] J. Mugnier, Jung, G. 1985. Survival of bacteria and fungi in relation to
water activity and the solvent properties of water in biopolymer gels.
Appl. Environ. Microbiol. 50: 1453-1458.
[15] R. J. Roughley, Gemell, L. G., Thompson, J. A., Brockwell, J. 1993. The
number of Bradyrhizobium sp.(Lupinus) applied to seed and its effect on
rhizosphere colonization, nodulation and yield of lupin. Soil Biol.
Biochem. 25: 1453-1458.
[16] M. P. Salema, Parker, C. A., Kiby, D. K. 1982. Death of rhizobia on
inoculated seed. Soil Biol. Biochem. 14: 13-14.
[17] P. Somasegaran, Hoben, H.J. 1994. Handbook for Rhizobia. New York:
Springer-Verlag.
[18] J. G. Streeter, 2003. Effect of trehalose on survival of Bradyrhizobium
japanicum during desiccation. J. Appl. Microbiol. 95: 484-491.
[19] J. G. Streeter, 2007. Factors affecting the survival of Bradyrhizobium
applied in liquid cultures to soya bean seeds. J. Appl. Microbiol. 103:
1282-1290.
[20] E. Tomb├ácz, Hajd├║, A., Illés. Water in contact with magnetite
nanoparticles, as seen from experiments and computer simulations. J.
Langmuir Articles., in press.
[21] J. A. C. Veriezen, De Bruijn, F., N├╝sslein, K. 2007. Responses of
rhizobia to desiccation in relation to osmotic stress, oxygen, and
temperature. J. Appl. Environ. Microbiol. 73: 3451-3459.
[22] A. G. Zavaglia, Tymczyszyn, E., De Antoni, G., Disalvo, E. A. 2003.
Action of trehalose on the preservation of Lactobacillus delbrueckii ssp.
bulgaricus by heat and osmotic dehydration. J. Appl. Microbiol. 95:
1315-1320.
@article{"International Journal of Biological, Life and Agricultural Sciences:58633", author = "M. R. Ghalamboran and J. J. Ramsden", title = "Viability of Bradyrhizobium japanicum on Soybean Seeds Enhanced by Magnetite Nanoparticles during Desiccation", abstract = "The aim of this study was to investigate whether
magnetite nanoparticles affect the viability of Bradyrhizobium
japanicum cells residing on the surface of soybean seeds during
desiccation. Different concentrations of nanoparticles suspended in
liquid medium, mixed with and adhering to Bradyrhizobium
japanicum, were investigated at two temperatures, using both
soybean seeds and glass beads as surrogates. Statistical design was a
complete randomized block (CRB) in a factorial 6×2×2×6
experimental arrangement with four replications. The most important
variable was the viability of Bradyrhizobium on the surface of the
seeds. The nanoparticles increased Bradyrhizobium viability and
inoculated seeds stored at low temperature had greater viability when
nanoparticles had been added. At the optimum nanoparticle
concentration, 50% bacterium viability on the seeds was retained
after 5 days at 4ºC. Possible explanations for the observed effects are
proposed.", keywords = "Bradyrhizobium japanicum, magnetitenanoparticles, soybean seed, viability.", volume = "4", number = "3", pages = "182-6", }