Characterization of Corn Cobs from Microwave and Potassium Hydroxide Pretreatment
The complexity of lignocellulosic biomass requires
a pretreatment step to improve the yield of fermentable sugars. The
efficient pretreatment of corn cobs using microwave and potassium
hydroxide and enzymatic hydrolysis was investigated. The
objective of this work was to characterize the optimal condition of
pretreatment of corn cobs using microwave and potassium
hydroxide enhance enzymatic hydrolysis. Corn cobs were
submerged in different potassium hydroxide concentration at varies
temperature and resident time. The pretreated corn cobs were
hydrolyzed to produce the reducing sugar for analysis. The
morphology and microstructure of samples were investigated by
Thermal gravimetric analysis (TGA, scanning electron microscope
(SEM), X-ray diffraction (XRD). The results showed that lignin
and hemicellulose were removed by microwave/potassium
hydroxide pretreatment. The crystallinity of the pretreated corn
cobs was higher than the untreated. This method was compared
with autoclave and conventional heating method. The results
indicated that microwave-alkali treatment was an efficient way to
improve the enzymatic hydrolysis rate by increasing its
accessibility hydrolysis enzymes.
[1] Carrillo F, Lis MJ, Colom X, Lo'pez-Mesas M, Valldeperas J. Effect
of alkali pretreatment on cellulase hydrolysis of wheat straw: Kinetic
study. Process Biochemistry 2005;40;3360-3364.
[2] Cheng J, Su H, Zhou J, Song W, Cen K. Microwave-assisted alkali
pretreatment of rice straw to promote enzymatic hydrolysis and
hydrogen production in dark- and photo-fermentation. International
Journal of Hydrogen Energy 2011;36;2093-2101.
[3] Zhu S, Wu Y, Yu Z, Liao J, Zhang Y. Pretreatment by
microwave/alkali of rice straw and its enzymic hydrolysis. Process
Biochemistry 2005; 40;3082-3086.
[4] Abdullah SS, Yusup S, Ahmad MM, Ramli A, Ismail L.
Thermogravimetry Study on Pyrolysis of Various Lignocellulosic
Biomass for Potential Hydrogen Production. International Journal of
Chemical and Biological Engineering 2010;72;129-133.
[5] Cao Y, Tan H. Study on crystal structures of enzyme-hydrolyzed
cellulosic materials by X-ray diffraction. Enzyme and Microbial
Technology 2005;36;314-317.
[6] Wang Z, Keshwani DR, Redding AP, Cheng JJ. Sodium hydroxide
pretreatment and enzymatic hydrolysis of coastal Bermuda grass.
Bioresource Technology 2010;101;3583-3585.
[7] Harmsen PFH, Huijgen WJJ, Berm├║dez L├│pez LM, Bakker RRC.
Literature Review of Physical and Chemical Pretreatment Process for
Lignocellulosic Biomass. Food & Biobased Research 2010;Accessed
on 9 May 2011http://www.ecn.nl/publicaties/PdfFetch.aspx?nr=ECNE--
10-013
[8] Zeng J, Singh D, Chen S. Biological pretreatment of wheat straw by
Phanerochaete chrysosporium supplemented with inorganic salts.
Bioresource Technology 2011;102; 3206-3214.
[9] Champagne P, Li C. Enzymatic hydrolysis of cellulosic municipal
wastewater treatment process residuals as feedstocks for the recovery
of simple sugars. Bioresource Technology 2009;100;5700-5706.
[10] Kuila A, Mukhopadhyay M, Tuli DK., Banerjee R. Production of
ethanol from lignicellulosics: an enzymatic venture. Experimental and
Clinical Sciences Journal 2011;85-96;1611-2156.
[1] Carrillo F, Lis MJ, Colom X, Lo'pez-Mesas M, Valldeperas J. Effect
of alkali pretreatment on cellulase hydrolysis of wheat straw: Kinetic
study. Process Biochemistry 2005;40;3360-3364.
[2] Cheng J, Su H, Zhou J, Song W, Cen K. Microwave-assisted alkali
pretreatment of rice straw to promote enzymatic hydrolysis and
hydrogen production in dark- and photo-fermentation. International
Journal of Hydrogen Energy 2011;36;2093-2101.
[3] Zhu S, Wu Y, Yu Z, Liao J, Zhang Y. Pretreatment by
microwave/alkali of rice straw and its enzymic hydrolysis. Process
Biochemistry 2005; 40;3082-3086.
[4] Abdullah SS, Yusup S, Ahmad MM, Ramli A, Ismail L.
Thermogravimetry Study on Pyrolysis of Various Lignocellulosic
Biomass for Potential Hydrogen Production. International Journal of
Chemical and Biological Engineering 2010;72;129-133.
[5] Cao Y, Tan H. Study on crystal structures of enzyme-hydrolyzed
cellulosic materials by X-ray diffraction. Enzyme and Microbial
Technology 2005;36;314-317.
[6] Wang Z, Keshwani DR, Redding AP, Cheng JJ. Sodium hydroxide
pretreatment and enzymatic hydrolysis of coastal Bermuda grass.
Bioresource Technology 2010;101;3583-3585.
[7] Harmsen PFH, Huijgen WJJ, Berm├║dez L├│pez LM, Bakker RRC.
Literature Review of Physical and Chemical Pretreatment Process for
Lignocellulosic Biomass. Food & Biobased Research 2010;Accessed
on 9 May 2011http://www.ecn.nl/publicaties/PdfFetch.aspx?nr=ECNE--
10-013
[8] Zeng J, Singh D, Chen S. Biological pretreatment of wheat straw by
Phanerochaete chrysosporium supplemented with inorganic salts.
Bioresource Technology 2011;102; 3206-3214.
[9] Champagne P, Li C. Enzymatic hydrolysis of cellulosic municipal
wastewater treatment process residuals as feedstocks for the recovery
of simple sugars. Bioresource Technology 2009;100;5700-5706.
[10] Kuila A, Mukhopadhyay M, Tuli DK., Banerjee R. Production of
ethanol from lignicellulosics: an enzymatic venture. Experimental and
Clinical Sciences Journal 2011;85-96;1611-2156.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:55435", author = "Boonyisa Wanitwattanarumlug and Apanee Luengnaruemitchai and Sujitra Wongkasemjit", title = "Characterization of Corn Cobs from Microwave and Potassium Hydroxide Pretreatment", abstract = "The complexity of lignocellulosic biomass requires
a pretreatment step to improve the yield of fermentable sugars. The
efficient pretreatment of corn cobs using microwave and potassium
hydroxide and enzymatic hydrolysis was investigated. The
objective of this work was to characterize the optimal condition of
pretreatment of corn cobs using microwave and potassium
hydroxide enhance enzymatic hydrolysis. Corn cobs were
submerged in different potassium hydroxide concentration at varies
temperature and resident time. The pretreated corn cobs were
hydrolyzed to produce the reducing sugar for analysis. The
morphology and microstructure of samples were investigated by
Thermal gravimetric analysis (TGA, scanning electron microscope
(SEM), X-ray diffraction (XRD). The results showed that lignin
and hemicellulose were removed by microwave/potassium
hydroxide pretreatment. The crystallinity of the pretreated corn
cobs was higher than the untreated. This method was compared
with autoclave and conventional heating method. The results
indicated that microwave-alkali treatment was an efficient way to
improve the enzymatic hydrolysis rate by increasing its
accessibility hydrolysis enzymes.", keywords = "Corn cobs, Enzymatic hydrolysis, Microwave,
Potassium hydroxide, Pretreatment.", volume = "6", number = "4", pages = "300-5", }