Product Development and Derivatives Exploration by using Photosynthetic Bacteria
Lycopene, which can be extracted from plants and is
very popular for fruit intake, is restricted for healthy food development
due to its high price. On the other hand, it will get great safety
concerns, especially in the food or cosmetic application, if the raw
material of lycopene is produced by chemical synthesis. In this
project, we provide a key technology to bridge the limitation as
mentioned above. Based on the abundant bioresources of BCRC
(Bioresource Collection and Research Center, Taiwan), a promising
lycopene output will be anticipated by the introduction of fermentation
technology along with industry-related core energy. Our results
showed that addition of tween 80(0.2%) and span 20 produced higher
amount of lycopene. And piperidine, when was added at 48hr to the
cultivation medium, could promote lycopene excretion effectively
also.
[1] H. Koku, I. Eroglu and U. Gunduz. "Kinetics of biological hydrogen
production by the photosynthetic bacterium Rhodobacter sphaeroides",
International Journal Hydrogen Energy , vol 28 (4), pp.381-388, 2003.
[2] Z. Penzkofer and S. Hegemann. "Absorption and fluorescence
spectroscopic characterization of BLUF domain of App.A from
Rhodobacter sphaeroides", Chemical Physiology, vol 315 (8),
pp.142-154, 2005
[3] Britton, G. "Overview of carotenoid biosynthesis"., Carotenoids Biosynthesis
and Metabolism, pp.143-147, 1998
[4] H.J.,Nelis, and A.P.DeLeeheer., "Microbial sources of carotenoid
pigments used in food and feeds", Journal of App.lied. Bacteriology, vol
70, pp. 181-191, 1991.
[5] Deming C.., Yonbin, H., and G. Zhenxin.. "Application of statistical
methodology to the optimization of fermentative medium for carotenoids
production by Rhodobacter sphaeroides.", Process Biochemistry, vol 41,
pp.1773-1778, 2006.
[6] T. W. Goodwin and D. G. " Studies in Carotenoid synthesis in the
photosynthetic bacterium Rhodospirillum sphaeroides." Biochemistry
Journal, vol 59, pp. 491-496,1955.
[7] M. L.Nguyen and S. J. Schwartz . "Lycopene: Chemical and biological
properties", Food Technology, vol 53, pp.38-45, 1999.
[8] J. K. Willcox, G. L. Catignani, and Lazarus, S. "Tomatoes and
cardiovascular diseases", Critical Review in Food Science and Nutrition,
vol 43, pp. 1-18, 2003.
[9] Arab, L., and S. Steck. "Lycopene and cardiovascular disease" , American
Journal of Clinical Nutrition, vol 71, pp.1691-1695, 2000.
[10] Assunta Z, Gianfranco C, Caterina L R., and Elena D. "Antioxidant
activity of tomato lipophilic extracts and interactions between carotenoids
and a-tocopherol in synthetic mixtures", LWT - Food Science and
Technology,vol 43,pp. 67-72, 2010.
[11] A. V. Rao, and Agarwal, S."Role of lycopene as antioxidant carotenoid in
the prevention of chronic diseases.", Nutrition Research, vol 19, pp.
305-323, 1999.
[12] Nutraingredients USA "Global carotenoid market to hit a new high",
<http:// www.nutraingredients.com/news/ print News Bis asp?id =784
87> , 2007.
[13] N.S.Govind, A.R. Amin, and V.V. Modi, " Stimulation of
carotenogenesis in Blakeslea trispora by cupric ions", Photochemistry,
vol 21, pp.1043-1044, 1982.
[14] X. Fang, Q.Yuan. and Zhu Y. "Improved production of lycopene and
β-carotene by Blakeslea trispora with oxygen-vectors", Process
Biochemistry, vol 42, pp. 289-293, 2007.
[15] Sheetal M. Laxmi A, Rekha S.and Singhal. "Use of metabolic
stimulators and inhibitors for enhanced production of β-carotene and
lycopene by Blakeslea trispora NRRL 2895 and 2896". Bioresource
Technology, vol 99, pp.3166-3173, 2008
[16] S.Kerr, J.Cal, and Cabral K." Factors enhancing lycopene production by
a new Mycobacterium aurum mutant", Biotechnology Letters, vol 26, pp.
103-108, 2004.
[17] P.Bhosale, "Environmental and cultural stimulants in the production of
carotenoids from microorganisms", Applied Microbiology
Biotechnology, vol l63, pp. 351-361, 2004.
[1] H. Koku, I. Eroglu and U. Gunduz. "Kinetics of biological hydrogen
production by the photosynthetic bacterium Rhodobacter sphaeroides",
International Journal Hydrogen Energy , vol 28 (4), pp.381-388, 2003.
[2] Z. Penzkofer and S. Hegemann. "Absorption and fluorescence
spectroscopic characterization of BLUF domain of App.A from
Rhodobacter sphaeroides", Chemical Physiology, vol 315 (8),
pp.142-154, 2005
[3] Britton, G. "Overview of carotenoid biosynthesis"., Carotenoids Biosynthesis
and Metabolism, pp.143-147, 1998
[4] H.J.,Nelis, and A.P.DeLeeheer., "Microbial sources of carotenoid
pigments used in food and feeds", Journal of App.lied. Bacteriology, vol
70, pp. 181-191, 1991.
[5] Deming C.., Yonbin, H., and G. Zhenxin.. "Application of statistical
methodology to the optimization of fermentative medium for carotenoids
production by Rhodobacter sphaeroides.", Process Biochemistry, vol 41,
pp.1773-1778, 2006.
[6] T. W. Goodwin and D. G. " Studies in Carotenoid synthesis in the
photosynthetic bacterium Rhodospirillum sphaeroides." Biochemistry
Journal, vol 59, pp. 491-496,1955.
[7] M. L.Nguyen and S. J. Schwartz . "Lycopene: Chemical and biological
properties", Food Technology, vol 53, pp.38-45, 1999.
[8] J. K. Willcox, G. L. Catignani, and Lazarus, S. "Tomatoes and
cardiovascular diseases", Critical Review in Food Science and Nutrition,
vol 43, pp. 1-18, 2003.
[9] Arab, L., and S. Steck. "Lycopene and cardiovascular disease" , American
Journal of Clinical Nutrition, vol 71, pp.1691-1695, 2000.
[10] Assunta Z, Gianfranco C, Caterina L R., and Elena D. "Antioxidant
activity of tomato lipophilic extracts and interactions between carotenoids
and a-tocopherol in synthetic mixtures", LWT - Food Science and
Technology,vol 43,pp. 67-72, 2010.
[11] A. V. Rao, and Agarwal, S."Role of lycopene as antioxidant carotenoid in
the prevention of chronic diseases.", Nutrition Research, vol 19, pp.
305-323, 1999.
[12] Nutraingredients USA "Global carotenoid market to hit a new high",
<http:// www.nutraingredients.com/news/ print News Bis asp?id =784
87> , 2007.
[13] N.S.Govind, A.R. Amin, and V.V. Modi, " Stimulation of
carotenogenesis in Blakeslea trispora by cupric ions", Photochemistry,
vol 21, pp.1043-1044, 1982.
[14] X. Fang, Q.Yuan. and Zhu Y. "Improved production of lycopene and
β-carotene by Blakeslea trispora with oxygen-vectors", Process
Biochemistry, vol 42, pp. 289-293, 2007.
[15] Sheetal M. Laxmi A, Rekha S.and Singhal. "Use of metabolic
stimulators and inhibitors for enhanced production of β-carotene and
lycopene by Blakeslea trispora NRRL 2895 and 2896". Bioresource
Technology, vol 99, pp.3166-3173, 2008
[16] S.Kerr, J.Cal, and Cabral K." Factors enhancing lycopene production by
a new Mycobacterium aurum mutant", Biotechnology Letters, vol 26, pp.
103-108, 2004.
[17] P.Bhosale, "Environmental and cultural stimulants in the production of
carotenoids from microorganisms", Applied Microbiology
Biotechnology, vol l63, pp. 351-361, 2004.
@article{"International Journal of Biological, Life and Agricultural Sciences:53936", author = "Yi-Fang Hung and Jinn-Tsyy Lai", title = "Product Development and Derivatives Exploration by using Photosynthetic Bacteria", abstract = "Lycopene, which can be extracted from plants and is
very popular for fruit intake, is restricted for healthy food development
due to its high price. On the other hand, it will get great safety
concerns, especially in the food or cosmetic application, if the raw
material of lycopene is produced by chemical synthesis. In this
project, we provide a key technology to bridge the limitation as
mentioned above. Based on the abundant bioresources of BCRC
(Bioresource Collection and Research Center, Taiwan), a promising
lycopene output will be anticipated by the introduction of fermentation
technology along with industry-related core energy. Our results
showed that addition of tween 80(0.2%) and span 20 produced higher
amount of lycopene. And piperidine, when was added at 48hr to the
cultivation medium, could promote lycopene excretion effectively
also.", keywords = "photosynthetic bacteria, lycopene, tween80,Piperidine", volume = "5", number = "9", pages = "525-3", }