Abstract: A comparative evaluation of acute toxicity of
synthesized nano silvers using two different procedures (biological
and chemical reduction methods) and silver ions on bacteria
Vibrio fischeri was investigated. The bacterial light inhibition test as
a toxicological endpoint was used by applying of a homemade
luminometer. To compare the toxicity effects as a quantitative
parameter, a nominal effective concentrations (EC) of chemicals and
a susceptibility constant (Z-value) of bacteria, after 5 min and 30 min
exposure times, were calculated. After 5 and 30 min contact times,
the EC50 values of two silver nanoparticles and the EC20 values were
about similar. It demonstrates that toxicity of silvers was independent
of their procedure. The EC values of nanoparticles were larger than
those of the silver ions. The susceptibilities(Z- Values) of V.fischeri
(L/mg) to the silver ions were greater than those of the nano silvers.
According to the EC and Z values, the toxicity of silvers decreased in
the following order: Silver ions >> silver nanoparticles from
chemical reduction method ~ silver nanoparticles from biological
method.
Abstract: The oleaginous yeasts Lipomyces starkey were grown
in the presence of dairy industry wastewaters (DIW). The yeasts were
able to degrade the organic components of DIW and to produce a
significant fraction of their biomass as triglycerides.
When using DIW from the Ricotta cheese production or residual
whey as growth medium, the L. starkey could be cultured without
dilution nor external organic supplement. On the contrary, the yeasts
could only partially degrade the DIW from the Mozzarella cheese
production, due to the accumulation of a metabolic product beyond
the threshold of toxicity. In this case, a dilution of the DIW was
required to obtain a more efficient degradation of the carbon
compounds and an higher yield in oleaginous biomass.
The fatty acid distribution of the microbial oils obtained showed a
prevalence of oleic acid, and is compatible with the production of a II
generation biodiesel offering a good resistance to oxidation as well as
an excellent cold-performance.
Abstract: The toxicity of aqueous extracts of two plants,
Nicotiana tobacum and Eucalyptus globulus were investigated against second instar larvae of Lycoriella auripila, one of the most
important pests of button mushroom, using agar dilution technique.
Seven concentrations of aqueous extracts of both plants were applied
on second instar larvae and their mortality were evaluated after 24, 48 and 72 h. The obtained results revealed that aqueous extracts of N.
tabacum and E. globulus caused 77.55 and 72.5% mortality of larvae
of L. auripila at concentration of 4000 ppm after 72h, respectively. Toxicities of tobacco extract after 24, 48 and 72 h were 1.52, 1.85
and 1.70 times greather than eucalyptus, respectively. The estimated LC50 after 24, 48 and 72 h were 7316.5, 2468.5 and 2013.1 ppm for
tobacco and 64870.0, 6839.5 and 3326.4 ppm for eucalyptus, respectively. These plants merit further study as potential insecticides
for the control of L. auripila.
Abstract: The possible therapeutic effect of cannabidiol, the major non-psychotropic Cannabis constituent, was investigated against acute hepatotoxicity induced by a single oral dose of acetaminophen (500mg/kg) in mice. Cannabidiol (two intraperitoneal injections, 5mg/kg, each) was given 1 hour and 12 hours following acetaminophen administration. Acetaminophen administration caused significant elevations of serum alanine aminotransferase, and hepatic malondialdehyde, and nitric oxide levels, and a significant decrease in hepatic reduced glutathione. Cannabidiol significantly attenuated the deterioration in the measured biochemical parameters resulted from acetaminophen administration. Also, histopathological examination showed that cannabidiol markedly attenuated ameliorated acetaminophen-induced liver tissue damage. These results emphasize that cannabidiol represents a potential therapeutic option to protect against acetaminophen hepartotoxicity which is a common clinical problem.
Abstract: Pyrite (FeS2) is a promising candidate for cathode
materials in batteries because of it`s high theoretical capacity, low
cost and non-toxicity. In this study, nano size iron disulfide thin film
was prepared on graphite substrate through a new method as battery
cathode. In this way, acetylene black and poly vinylidene fluoride
were used as electron conductor and binder, respectively. Fabricated
thin films were analyzed by XRD and SEM. These results and
electrochemical data confirm improvement of battery discharge
capacity in comparison with commercial type of pyrite.
Abstract: Bottle water is getting very popular all through the
world; especially in the gulf countries as the main source of drinking
water. However, concerns over leaching of toxic chemicals are
increasing. In this study, a health risk assessment was conducted in
accordance with the guidelines indicated by United States
Environmental Protection Agency (USEPA). It is conducted based on
leaching of Diethyl Phthalate (DEP) from Polyethylene terephthalate
(PET). The toxicity and exposure assessment of diethyl phthalate was
conducted to characterize its risk on human health. Risk management
is also discussed.
Abstract: The agriculture lignocellulosic by-products are receiving increased attention, namely in the search for filter materials that retain contaminants from water. These by-products, specifically almond and hazelnut shells are abundant in Portugal once almond and hazelnuts production is a local important activity. Hazelnut and almond shells have as main constituents lignin, cellulose and hemicelluloses, water soluble extractives and tannins. Along the adsorption of heavy metals from contaminated waters, water soluble compounds can leach from shells and have a negative impact in the environment. Usually, the chemical characterization of treated water by itself may not show environmental impact caused by the discharges when parameters obey to legal quality standards for water. Only biological systems can detect the toxic effects of the water constituents. Therefore, the evaluation of toxicity by biological tests is very important when deciding the suitability for safe water discharge or for irrigation applications.
The main purpose of the present work was to assess the potential impacts of waters after been treated for heavy metal removal by hazelnut and almond shells adsorption systems, with short term acute toxicity tests.
To conduct the study, water at pH 6 with 25 mg.L-1 of lead, was treated with 10 g of shell per litre of wastewater, for 24 hours. This procedure was followed for each bark. Afterwards the water was collected for toxicological assays; namely bacterial resistance, seed germination, Lemna minor L. test and plant grow. The effect in isolated bacteria strains was determined by disc diffusion method and the germination index of seed was evaluated using lettuce, with temperature and humidity germination control for 7 days. For aquatic higher organism, Lemnas were used with 4 days contact time with shell solutions, in controlled light and temperature. For terrestrial higher plants, biomass production was evaluated after 14 days of tomato germination had occurred in soil, with controlled humidity, light and temperature.
Toxicity tests of water treated with shells revealed in some extent effects in the tested organisms, with the test assays showing a close behaviour as the control, leading to the conclusion that its further utilization may not be considered to create a serious risk to the environment.
Abstract: Chitosan is an attractive polysaccharide obtained by
deacetylation of an abundant natural biopolymer called chitin. Chitin
and chitosan are excellent materials. To improve the potential of
chitin and chitosan modification is needed. In the present study,
grafting of maleic acid on to chitosan by cerium ammonium nitrate in
acetic acid solution was investigated with use of a microwave and
reflux system. The grafted chitosan was characterized by using a
Fourier-transform infrared spectrometry. The solubility and swelling
behavior of grafted chitosans were determined in acetate buffer (pH
3.6), citrophosphate buffer (pH 5.6 and pH 7.0), and boric buffer (pH
9.2) solutions. The sample obtained by microwave system with use of
a chitosan/maleic anhydride/ceric ammonium nitrate 0.2/3.922/0.99
gram of raw material within 30 minute showed the maximum
swelling ratio (13.6) in boric buffer solution.
Abstract: The characterization of κ-carrageenan could provide a
better understanding of its functions in biological, medical and
industrial applications. Chemical and physical analyses of
carrageenan from seaweeds, Euchema cottonii L., were done to offer
information on its properties and the effects of Co-60 γ-irradiation on
its thermochemical characteristics. The structural and morphological
characteristics of κ-carrageenan were determined using scanning
electron microscopy (SEM) while the composition, molecular weight
and thermal properties were determined using attenuated total
reflectance Fourier transform infrared spectroscopy (ATR-FTIR), gel
permeation chromatography (GPC), thermal gravimetric analysis
(TGA) and differential scanning calorimetry (DSC). Further chemical
analysis was done using hydrogen-1 nuclear magnetic resonance (1H
NMR) and functional characteristics in terms of biocompatibility
were evaluated using cytotoxicity test.
Abstract: The antimicrobial, antiplasmid and cytotoxic activities of marine algae Halimeda opuntia and Sarconema filiforme were investigated. Antimicrobial bioassay against some human pathogenic bacteria and yeast were conducted using disc diffusion method. Halimeda extract exhibited antibacterial activity against six species of microrganisms, with significant inhibition against Staphylococcus aureus. While Sarconema extract was better potent as antifungal against Candida albicans. Comparative antibacterial studies showed that Halimeda extract showed equivalent or better activity as compared with commercial antibiotic when tested against Staphylococcus aureus. Further tests conducted using dilution method showed both extracts as having bacteriostatic mode of action against the tested microorganisms. Methanol extract of two species showed significant cytotoxicity (LC50
Abstract: Sensitive and predictive DILI (Drug Induced Liver
Injury) biomarkers are needed in drug R&D to improve early
detection of hepatotoxicity. The discovery of DILI biomarkers that
demonstrate the predictive power to identify individuals at risk to
DILI would represent a major advance in the development of
personalized healthcare approaches. In this healthy volunteer
acetaminophen study (4g/day for 7 days, with 3 monitored nontreatment
days before and 4 after), 450 serum samples from 32
subjects were analyzed using protein profiling by antibody
suspension bead arrays. Multiparallel protein profiles were generated
using a DILI target protein array with 300 antibodies, where the
antibodies were selected based on previous literature findings of
putative DILI biomarkers and a screening process using pre dose
samples from the same cohort. Of the 32 subjects, 16 were found to
develop an elevated ALT value (2Xbaseline, responders). Using the
plasma profiling approach together with multivariate statistical
analysis some novel findings linked to lipid metabolism were found
and more important, endogenous protein profiles in baseline samples
(prior to treatment) with predictive power for ALT elevations were
identified.
Abstract: Phytotoxicity of Daphne gnidium L. was evaluated
through the effect of incorporating leaves, stems and roots biomass
into soil (at 12.5, 25, 50g/Kg) and irrigation by their aqueous extracts
(50g/L), on the growth of two crops (Lactuca sativa L. and Raphanus
sativus L.) and two weeds (Peaganum harmala L. and Scolymus
maculatus L.). Results revealed a perceptible phytotoxic effect which
increased with dose and concentration. At the highest dose, roots and
leaves residues was the most toxic and caused total inhibition
respectively, for lettuce and thistle seedling growth. Irrigation with
aqueous extracts of D. gnidium different organs decreased also
seedlings length of all test species. Stems extract was more inhibitor
on thistle than peganum seedling growth; it induced a significant
reduction of 80% and 67%, for, respectively, roots and shoots.
Results of the present study suggest that different organs of D.
gnidium could be exploited in the management of agro-ecosystems.
Abstract: This study investigated a strategy of blending lead-laden sludge and Al-rich precursors to reduce the release of metals from the stabilized products. Using PbO as the simulated lead-laden sludge to sinter with γ-Al2O3 by Pb:Al molar ratios of 1:2 and 1:12, PbAl2O4 and PbAl12O19 were formed as final products during the sintering process, respectively. By firing the PbO + γ-Al2O3 mixtures with different Pb/Al molar ratios at 600 to 1000 °C, the lead transformation was determined through X-ray diffraction (XRD) data. In Pb/Al molar ratio of 1/2 system, the formation of PbAl2O4 is initiated at 700 °C, but an effective formation was observed above 750 °C. An intermediate phase, Pb9Al8O21, was detected in the temperature range of 800-900 °C. However, different incorporation behavior for sintering PbO with Al-rich precursors at a Pb/Al molar ratio of 1/12 was observed during the formation of PbAl12O19 in this system. In the sintering process, both temperature and time effect on the formation of PbAl2O4 and PbAl12O19 phases were estimated. Finally, a prolonged leaching test modified from the U.S. Environmental Protection Agency-s toxicity characteristic leaching procedure (TCLP) was used to evaluate the durability of PbO, Pb9Al8O21, PbAl2O4 and PbAl12O19 phases. Comparison for the leaching results of the four phases demonstrated the higher intrinsic resistance of PbAl12O19 against acid attack.
Abstract: Grey mold on grape is caused by the fungus Botrytis
cinerea Pers. Trichodex WP, a new biofungicide, that contains fungal
spores of Trichoderma harzianum Rifai, was used for biological
control of Grey mold on grape. The efficacy of Trichodex WP has
been reported from many experiments. Experiments were carried out
in the locality – Banatski Karlovac, on grapevine species – talijanski
rizling. The trials were set according to instructions of methods
PP1/152(2) and PP1/17(3) , according to a fully randomized block
design. Phytotoxicity was estimated by PP methods 1/135(2), the
intensity of infection according to Towsend Heuberger , the
efficiency by Abbott, the analysis of variance with Duncan test and
PP/181(2). Application of Trichodex WP is limited to the first two
treatments. Other treatments are performed with the fungicides based
on a.i. procymidone, vinclozoline and iprodione.
Abstract: Vernonia divergens Benth., commonly known as
“Insulin Plant” (Fam: Asteraceae) is a potent sugar killer. Locally the
leaves of the plant, boiled in water are successfully administered to a
large number of diabetic patients. The present study evaluates the
putative anti-diabetic ingredients, isolated from the in vivo and in
vitro grown plantlets of V. divergens for their antimicrobial and
anticancer activities. Sterilized explants of nodal segments were
cultured on MS (Musashige and Skoog, 1962) medium in presence of
different combinations of hormones. Multiple shoots along with
bunch of roots were regenerated at 1mg l-1 BAP and 0.5 mg l-1 NAA.
Micro-plantlets were separated and sub-cultured on the double
strength (2X) of the above combination of hormones leading to
increased length of roots and shoots. These plantlets were
successfully transferred to soil and survived well in nature. The
ethanol extract of plantlets from both in vivo & in vitro sources were
prepared in soxhlet extractor and then concentrated to dryness under
reduced pressure in rotary evaporator. Thus obtainedconcentrated
extracts showed significant inhibitory activity against gram
negative bacteria like Escherichia coli and Pseudomonas
aeruginosa but no inhibition was found against gram positive
bacteria. Further, these ethanol extracts were screened for in vitro
percentage cytotoxicity at different time periods (24 h, 48 h and 72 h)
of different dilutions. The in vivo plant extract inhibited the growth of
EAC mouse cell lines in the range of 65, 66, 78, and 88% at 100, 50,
25 & 12.5μg mL-1 but at 72 h of treatment. In case of the extract of in
vitro origin, the inhibition was found against EAC cell lines even at
48h. During spectrophotometric scanning, the extracts exhibited
different maxima (ʎ) - four peaks in in vitro extracts as against single
in in vivo preparation suggesting the possible change in the nature of
ingredients during micropropagation through tissue culture
techniques.
Abstract: Biological reactions of individuals of a testing animal
to toxic substance are unique and can be used as an indication of the
existing of toxic substance. However, to distinguish such phenomenon
need a very complicate system and even more complicate to analyze
data in 3 dimensional. In this paper, a system to evaluate in vitro
biological activities to acute toxicity of stochastic self-affine
non-stationary signal of 3D goldfish swimming by using fractal
analysis is introduced. Regular digital camcorders are utilized by
proposed algorithm 3DCCPC to effectively capture and construct 3D
movements of the fish. A Critical Exponent Method (CEM) has been
adopted as a fractal estimator. The hypothesis was that the swimming
of goldfish to acute toxic would show the fractal property which
related to the toxic concentration. The experimental results supported
the hypothesis by showing that the swimming of goldfish under the
different toxic concentration has fractal properties. It also shows that
the fractal dimension of the swimming related to the pH value of FD Ôëê
0.26pH + 0.05. With the proposed system, the fish is allowed to swim
freely in all direction to react to the toxic. In addition, the trajectories
are precisely evaluated by fractal analysis with critical exponent
method and hence the results exhibit with much higher degree of
confidence.
Abstract: Magnesium is used implant material potentially for
non-toxicity to the human body. Due to the excellent
bio-compatibility, Mg alloys is applied to implants avoiding removal
second surgery. However, it is found commercial magnesium alloys
including aluminum has low corrosion resistance, resulting
subcutaneous gas bubbles and consequently the approach as
permanent bio-materials. Generally, Aluminum is known to pollution
substance, and it raises toxicity to nervous system. Therefore
especially Mg-35Zn-3Ca alloy is prepared for new biodegradable
materials in this study. And the pulsed power is used in
constant-current mode of DC power kinds of anodization. Based on
the aforementioned study, it examines corrosion resistance and
biocompatibility by effect of current and frequency variation. The
surface properties and thickness were compared using scanning
electronic microscopy. Corrosion resistance was assessed via
potentiodynamic polarization and the effect of oxide layer on the body
was assessed cell viability. Anodized Mg-35Zn-3Ca alloy has good
biocompatibility in vitro by current and frequency variation.
Abstract: In production of medicinal plants, seed germination is
very important problem. The treated seeds (control, hydro priming
and ZnSO4) of Cumin (Cuminum cyminum L.) were evaluated at
germination and seedling growth for tolerance to salt (NaCl and
Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, -
0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl
solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. The
objective of the study was to determine factors responsible for
germination and early seedling growth due to salt toxicity or osmotic
effect and to optimize the best priming treatment for these stress
conditions. Results revealed that germination delayed in both
solutions, having variable germination with different priming
treatments. Germination, shoot and weight, root and shoot length
were higher but mean germination time and abnormal germination
percentage were lower in NaCl than Na2SO4 at the same water
potential. The root / shoot weight and R/S length increased with
increase in osmotic potential in both NaCl and Na2SO4 solutions.
NaCl had less inhibitor effect on seedling growth than the
germination. It was concluded that inhibition of germination at the
same water potential of NaCl and Na2SO4 resulted from salt toxicity
rather than osmotic effect. Hydro priming increased germination and
seedling growth under salt stress. This protocol has practical
importance and could be recommended to farmers to achieve higher
germination and uniform emergence under field conditions.
Abstract: Magnetic and semiconductor nanomaterials exhibit
novel magnetic and optical properties owing to their unique size and
shape-dependent effects. With shrinking the size down to nanoscale
region, various anomalous properties that normally not present in bulk
start to dominate. Ability in harnessing of these anomalous properties
for the design of various advance electronic devices is strictly
dependent on synthetic strategies. Hence, current research has focused
on developing a rational synthetic control to produce high quality
nanocrystals by using organometallic approach to tune both size and
shape of the nanomaterials. In order to elucidate the growth
mechanism, transmission electron microscopy was employed as a
powerful tool in performing real time-resolved morphologies and
structural characterization of magnetic (Fe3O4) and semiconductor
(ZnO) nanocrystals. The current synthetic approach is found able to
produce nanostructures with well-defined shapes. We have found that
oleic acid is an effective capping ligand in preparing oxide-based
nanostructures without any agglomerations, even at high temperature.
The oleate-based precursors and capping ligands are fatty acid
compounds, which are respectively originated from natural palm oil
with low toxicity. In comparison with other synthetic approaches in
producing nanostructures, current synthetic method offers an effective
route to produce oxide-based nanomaterials with well-defined shapes
and good monodispersity. The nanocystals are well-separated with
each other without any stacking effect. In addition, the as-synthesized
nanopellets are stable in terms of chemically and physically if
compared to those nanomaterials that are previous reported. Further
development and extension of current synthetic strategy are being
pursued to combine both of these materials into nanocomposite form
that will be used as “smart magnetic nanophotocatalyst" for industry
waste water treatment.
Abstract: The removal efficiency of 4-chlorophenol with
different advanced oxidation processes have been studied. Oxidation
experiments were carried out using two 4-chlorophenol
concentrations: 100 mg L-1 and 250 mg L-1 and UV generated from a
KrCl excilamp with (molar ratio H2O2: 4-chlorophenol = 25:1) and
without H2O2, and, with Fenton process (molar ratio H2O2:4-
chlorophenol of 25:1 and Fe2+ concentration of 5 mg L-1).
The results show that there is no significant difference in the 4-
chlorophenol conversion when using one of the three assayed
methods. However, significant concentrations of the photoproductos
still remained in the media when the chosen treatment involves UV
without hydrogen peroxide. Fenton process removed all the
intermediate photoproducts except for the hydroquinone and the
1,2,4-trihydroxybenzene. In the case of UV and hydrogen peroxide
all the intermediate photoproducts are removed.
Microbial bioassays were carried out utilising the naturally
luminescent bacterium Vibrio fischeri and a genetically modified
Pseudomonas putida isolated from a waste treatment plant receiving
phenolic waste. The results using V. fischeri show that with samples
after degradation, only the UV treatment showed toxicity (IC50 =38)
whereas with H2O2 and Fenton reactions the samples exhibited no
toxicity after treatment in the range of concentrations studied. Using
the Pseudomonas putida biosensor no toxicity could be detected for
all the samples following treatment due to the higher tolerance of the
organism to phenol concentrations encountered.