Culture of Oleaginous Yeasts in Dairy Industry Wastewaters to Obtain Lipids Suitable for the Production of II-Generation Biodiesel
The oleaginous yeasts Lipomyces starkey were grown
in the presence of dairy industry wastewaters (DIW). The yeasts were
able to degrade the organic components of DIW and to produce a
significant fraction of their biomass as triglycerides.
When using DIW from the Ricotta cheese production or residual
whey as growth medium, the L. starkey could be cultured without
dilution nor external organic supplement. On the contrary, the yeasts
could only partially degrade the DIW from the Mozzarella cheese
production, due to the accumulation of a metabolic product beyond
the threshold of toxicity. In this case, a dilution of the DIW was
required to obtain a more efficient degradation of the carbon
compounds and an higher yield in oleaginous biomass.
The fatty acid distribution of the microbial oils obtained showed a
prevalence of oleic acid, and is compatible with the production of a II
generation biodiesel offering a good resistance to oxidation as well as
an excellent cold-performance.
[1] Y. Christi, "Biodiesel production from microalgae", Biotechnol. Adv.,
vol. 25, pp. 294-306, 2007.
[2] Y.D. You, J.L. Shie, C.Y. Chang, S.H. Huang, C.Y. Pai, Y.H. Yu, and
C.J. Chang, "Economic cost analysis of biodiesel production: case in
soybean oil", Energy & Fuels, vol. 22, pp. 182-189, 2008.
[3] B. Liu, and Z.K. Zhao, "Biodiesel production by direct methanolysis of
oleaginous microbial biomass", J. Chem. Technol. Biotechnol., vol.
82(8), pp. 775-780, 2007.
[4] Y.H. Li, Z.B. Zhao, and F.W. Bai, "High-density cultivation of
oleaginous yeasts Rhodosporidium toruloides Y4 in fed-batch culture",
Enzyme Microb. Technol., vol. 41, pp. 312-317, 2007.
[5] C.C. Dai, J. Tao, F. Xie, Y.J. Dai, and M. Zhao, "Biodiesel generation
from oleaginous yeast Rhodotorula glutinis with xylose assimilating
capacity", African J. Biotechnol., vol. 6, pp. 2130-2134, 2007.
[6] C. Angerbauer, M. Siebenhofer, M. Mittelbach, and G.M. Guebitzn,
"Conversion of sewage sludge into lipids by Lipomyces starkeyi for
biodiesel production", Biores. Technol., vol. 99, pp. 3051-3056, 2008.
[7] S. Papanikolaou and G. Aggelis, "Lipids of oleaginous yeasts. Part I:
Biochemistry of single cell oil production", Eur. J. Lipid Sci. Technol.
vol. 113, pp. 1031-1051, 2011.
[8] S. Papanikolaou and G. Aggelis, "Lipids of oleaginous yeasts. Part II:
Technology and potential applications", Eur. J. Lipid Sci. Technol. vol.
113, pp. 1052-1073, 2011.
[9] J.E. Holdsworth, M. Veenhuis, and C. Ratledge, "Enzyme activities in
oleaginous yeasts accumulating and utilizing exogenous or endogenous
lipids", J. Gen. Microbiol., vol. 134, pp. 2907-2915, 1988.
[1] Y. Christi, "Biodiesel production from microalgae", Biotechnol. Adv.,
vol. 25, pp. 294-306, 2007.
[2] Y.D. You, J.L. Shie, C.Y. Chang, S.H. Huang, C.Y. Pai, Y.H. Yu, and
C.J. Chang, "Economic cost analysis of biodiesel production: case in
soybean oil", Energy & Fuels, vol. 22, pp. 182-189, 2008.
[3] B. Liu, and Z.K. Zhao, "Biodiesel production by direct methanolysis of
oleaginous microbial biomass", J. Chem. Technol. Biotechnol., vol.
82(8), pp. 775-780, 2007.
[4] Y.H. Li, Z.B. Zhao, and F.W. Bai, "High-density cultivation of
oleaginous yeasts Rhodosporidium toruloides Y4 in fed-batch culture",
Enzyme Microb. Technol., vol. 41, pp. 312-317, 2007.
[5] C.C. Dai, J. Tao, F. Xie, Y.J. Dai, and M. Zhao, "Biodiesel generation
from oleaginous yeast Rhodotorula glutinis with xylose assimilating
capacity", African J. Biotechnol., vol. 6, pp. 2130-2134, 2007.
[6] C. Angerbauer, M. Siebenhofer, M. Mittelbach, and G.M. Guebitzn,
"Conversion of sewage sludge into lipids by Lipomyces starkeyi for
biodiesel production", Biores. Technol., vol. 99, pp. 3051-3056, 2008.
[7] S. Papanikolaou and G. Aggelis, "Lipids of oleaginous yeasts. Part I:
Biochemistry of single cell oil production", Eur. J. Lipid Sci. Technol.
vol. 113, pp. 1031-1051, 2011.
[8] S. Papanikolaou and G. Aggelis, "Lipids of oleaginous yeasts. Part II:
Technology and potential applications", Eur. J. Lipid Sci. Technol. vol.
113, pp. 1052-1073, 2011.
[9] J.E. Holdsworth, M. Veenhuis, and C. Ratledge, "Enzyme activities in
oleaginous yeasts accumulating and utilizing exogenous or endogenous
lipids", J. Gen. Microbiol., vol. 134, pp. 2907-2915, 1988.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:60365", author = "Domenico Pirozzi and Angelo Ausiello and Gaetano Zuccaro and Filomena Sannino and Abu Yousuf", title = "Culture of Oleaginous Yeasts in Dairy Industry Wastewaters to Obtain Lipids Suitable for the Production of II-Generation Biodiesel", abstract = "The oleaginous yeasts Lipomyces starkey were grown
in the presence of dairy industry wastewaters (DIW). The yeasts were
able to degrade the organic components of DIW and to produce a
significant fraction of their biomass as triglycerides.
When using DIW from the Ricotta cheese production or residual
whey as growth medium, the L. starkey could be cultured without
dilution nor external organic supplement. On the contrary, the yeasts
could only partially degrade the DIW from the Mozzarella cheese
production, due to the accumulation of a metabolic product beyond
the threshold of toxicity. In this case, a dilution of the DIW was
required to obtain a more efficient degradation of the carbon
compounds and an higher yield in oleaginous biomass.
The fatty acid distribution of the microbial oils obtained showed a
prevalence of oleic acid, and is compatible with the production of a II
generation biodiesel offering a good resistance to oxidation as well as
an excellent cold-performance.", keywords = "Yeasts, Lipids, Biodiesel, Dairy industry
wastewaters.", volume = "7", number = "4", pages = "196-5", }