Abstract: Characterization and evaluation of the activity of Vespa basalis DPP-IV, which expressed in Spodoptera frugiperda 21 cells. The expression of rDPP-IV was confirmed by SDS–PAGE, Western blot analyses, LC-MS/MS and measurement of its peptidase specificity. One-step purification by Ni-NTA affinity chromatography and the total amount of rDPP-IV recovered was approximately 6.4mg per liter from infected culture medium; an equivalent amount would be produced by 1x109 infected Sf21 insect cells. Through the affinity purification led to highly stable rDPP-IV enzyme was recovered and with significant peptidase activity. The rDPP-IV exhibited classical Michaelis–Menten kinetics, with kcat/Km in the range of 10-500 mM-1×S-1 for the five synthetic substrates and optimum substrate is Ala-Pro-pNA. As expected in inhibition assay, the enzymatic activity of rDPP-IV was significantly reduced by 80 or 60% in the presence of sitagliptin (a DPP-IV inhibitor) or PMSF (a serine protease inhibitor), but was not apparently affected by iodoacetamide (a cysteine protease inhibitor).
Abstract: Carbon nanotubes (CNTs) possess unique structural,
mechanical, thermal and electronic properties, and have been
proposed to be used for applications in many fields. However, to
reach the full potential of the CNTs, many problems still need to be
solved, including the development of an easy and effective
purification procedure, since synthesized CNTs contain impurities,
such as amorphous carbon, carbon nanoparticles and metal particles.
Different purification methods yield different CNT characteristics
and may be suitable for the production of different types of CNTs. In
this study, the effect of different purification chemicals on carbon
nanotube quality was investigated. CNTs were firstly synthesized by
chemical vapor deposition (CVD) of acetylene (C2H2) on a
magnesium oxide (MgO) powder impregnated with an iron nitrate
(Fe(NO3)3·9H2O) solution. The synthesis parameters were selected
as: the synthesis temperature of 800°C, the iron content in the
precursor of 5% and the synthesis time of 30 min. The liquid phase
oxidation method was applied for the purification of the synthesized
CNT materials. Three different acid chemicals (HNO3, H2SO4, and
HCl) were used in the removal of the metal catalysts from the
synthesized CNT material to investigate the possible effects of each
acid solution to the purification step. Purification experiments were
carried out at two different temperatures (75 and 120 °C), two
different acid concentrations (3 and 6 M) and for three different time
intervals (6, 8 and 15 h). A 30% H2O2 : 3M HCl (1:1 v%) solution
was also used in the purification step to remove both the metal
catalysts and the amorphous carbon. The purifications using this
solution were performed at the temperature of 75°C for 8 hours.
Purification efficiencies at different conditions were evaluated by
thermogravimetric analysis. Thermal and electrical properties of
CNTs were also determined. It was found that the obtained electrical
conductivity values for the carbon nanotubes were typical for organic
semiconductor materials and thermal stabilities were changed
depending on the purification chemicals.
Abstract: A laboratory set-up was designed to survey the
effectiveness of UV/O3 advanced oxidation process (AOP) for the
removal of Carbaryl from polluted water in batch reactor. The study
was carried out by UV/O3 process for water samples containing 1 to
20 mg/L of Carbaryl in distilled water. Also the range of drinking
water resources adjusted in synthetic water and effects of contact
time, pH and Carbaryl concentration were studied. The residual
pesticide concentration was determined by applying high
performance liquid chromatography (HPLC). The results indicated
that increasing of retention time and pH, enhances pesticide removal
efficiency. The removal efficiency has been affected by pesticide
initial concentration. Samples with low pesticide concentration
showed a remarkable removal efficiency compared to the samples
with high pesticide concentration. AOP method showed the removal
efficiencies of 80% to 100%. Although process showed high
performance for removal of pesticide from water samples, this
process has different disadvantages including complication,
intolerability, difficulty of maintenance and equipmental and
structural requirements.
Abstract: The main objective of this study was to remove and recover Ni, Cu and Fe from a mixed metal system using sodium hypophosphite as a reducing agent and nickel powder as seeding material. The metal systems studied consisted of Ni-Cu, Ni-Fe and Ni-Cu-Fe solutions. A 5 L batch reactor was used to conduct experiments where 100 mg/l of each respective metal was used. It was found that the metals were reduced to their elemental form with removal efficiencies of over 80%. The removal efficiency decreased in the order Fe>Ni>Cu. The metal powder obtained contained between 97-99% Ni and was almost spherical and porous. Size enlargement by aggregation was the dominant particulate process.
Abstract: Nowadays, web-based technologies influence in
people-s daily life such as in education, business and others.
Therefore, many web developers are too eager to develop their web
applications with fully animation graphics and forgetting its
accessibility to its users. Their purpose is to make their web
applications look impressive. Thus, this paper would highlight on the
usability and accessibility of a voice recognition browser as a tool to
facilitate the visually impaired and blind learners in accessing virtual
learning environment. More specifically, the objectives of the study
are (i) to explore the challenges faced by the visually impaired
learners in accessing virtual learning environment (ii) to determine
the suitable guidelines for developing a voice recognition browser
that is accessible to the visually impaired. Furthermore, this study
was prepared based on an observation conducted with the Malaysian
visually impaired learners. Finally, the result of this study would
underline on the development of an accessible voice recognition
browser for the visually impaired.
Abstract: There are few studies on eggshell of leatherback turtle
which is endangered species in Thailand. This study was focusing on
the ultrastructure and elemental composition of leatherback turtle
eggshells collected from Andaman Sea Shore, Thailand during the
nesting season using scanning electron microscope (SEM). Three
eggshell layers of leatherback turtle; the outer cuticle layer or
calcareous layer, the middle layer or middle multistrata layer and the
inner fibrous layer were recognized. The outer calcareous layer was
thick and porosity which consisted of loose nodular units of various
crystal shapes and sizes. The loose attachment between these units
resulted in numerous spaces and openings. The middle layer was
compact thick with several multistrata and contained numerous
openings connecting to both outer cuticle layer and inner fibrous
layer. The inner fibrous layer was compact and thin, and composed of
numerous reticular fibers. Energy dispersive X-ray microanalysis
detector revealed energy spectrum of X-rays character emitted from
all elements on each layer. The percentages of all elements were
found in the following order: carbon (C) > oxygen (O) > calcium
(Ca) > sulfur (S) > potassium (K) > aluminum (Al) > iodine (I) >
silicon (Si) > chlorine (Cl) > sodium (Na) > fluorine (F) >
phosphorus (P) > magnesium (Mg). Each layer consisted of high
percentage of CaCO3 (approximately 98%) implying that it was
essential for turtle embryonic development. A significant difference
was found in the percentages of Ca and Mo in the 3layers. Moreover,
transition metal, metal and toxic non-metal contaminations were
found in leatherback turtle eggshell samples. These were palladium
(Pd), molybdenum (Mo), copper (Cu), aluminum (Al), lead (Pb), and
bromine (Br). The contamination elements were seen in the outer
layers except for Mo. All elements were readily observed and
mapped using Smiling program. X-ray images which mapped the
location of all elements were showed. Calcium containing in the
eggshell appeared in high contents and was widely distributing in
clusters of the outer cuticle layer to form CaCO3 structure. Moreover,
the accumulation of Na and Cl was observed to form NaCl which was
widely distributing in 3 eggshell layers. The results from this study
would be valuable on assessing the emergent success in this
endangered species.
Abstract: Coagulation of water involves the use of coagulating
agents to bring the suspended matter in the raw water together for
settling and the filtration stage. Present study is aimed to examine the
effects of aluminum sulfate as coagulant in conjunction with Moringa
Oleifera Coagulant Protein as coagulant aid on turbidity, hardness,
and bacteria in turbid water. A conventional jar test apparatus was
employed for the tests. The best removal was observed at a pH of 7
to 7.5 for all turbidities. Turbidity removal efficiency was resulted
between % 80 to % 99 by Moringa Oleifera Coagulant Protein as
coagulant aid. Dosage of coagulant and coagulant aid decreased with
increasing turbidity. In addition, Moringa Oleifera Coagulant Protein
significantly has reduced the required dosage of primary coagulant.
Residual Al+3 in treated water were less than 0.2 mg/l and meets the
environmental protection agency guidelines. The results showed that
turbidity reduction of % 85.9- % 98 paralleled by a primary
Escherichia coli reduction of 1-3 log units (99.2 – 99.97%) was
obtained within the first 1 to 2 h of treatment. In conclusions,
Moringa Oleifera Coagulant Protein as coagulant aid can be used for
drinking water treatment without the risk of organic or nutrient
release. We demonstrated that optimal design method is an efficient
approach for optimization of coagulation-flocculation process and
appropriate for raw water treatment.
Abstract: A double module hollow fiber supported liquid
membrane (HFSLM) was applied to selectively separate lead and
mercury ions from dilute synthetic produced water. The experiments
were investigated on several variables: types of extractants
(D2EHPA, Cyanex 471, Aliquat 336, and TOA), concentration of the
selected extractant and operating time. The results clearly showed
that the double module HFSLM could selectively separate Pb(II) and
Hg(II) in feed solution at a very low concentration to less than the
regulatory discharge limit of 0.2 and 0.005 mg/L issued by the
Ministry of Industry and the Ministry of Natural Resource
Environment, Thailand. The highest extractions of lead and mercury
ions from synthetic produced water were 96% and 100% using 0.03
M D2EHPA and 0.06 M Aliquat 336 as the extractant for the first
and second modules.
Abstract: Residues are produced in all stages of human activities
in terms of composition and volume which vary according to
consumption practices and to production methods. Forms of
significant harm to the environment are associated to volume of
generated material as well as to improper disposal of solid wastes,
whose negative effects are noticed more frequently in the long term.
The solution to this problem constitutes a challenge to the
government, industry and society, because they involve economic,
social, environmental and, especially, awareness of the population in
general. The main concerns are focused on the impact it can have on
human health and on the environment (soil, water, air and sights).
The hazardous waste produced mainly by industry, are particularly
worrisome because, when improperly managed, they become a
serious threat to the environment. In view of this issue, this study
aimed to evaluate the management system of solid waste of a coprocessing
industrial waste company, to propose improvements to the
rejects generation management in a specific step of the Blending
production process.
Abstract: In composting process, N high-organic wastes loss the
great part of its nitrogen as ammonia; therefore, using compost
amendments can promote the quality of compost due to the decrease
in ammonia volatilization. With regard to the effect of pH on
composting, microorganisms- activity and ammonia volatilization,
sulfuric acid and alkaline wastewater of paper mill (as liming agent
with Ca and Mg ions) were used as compost amendments. Study
results indicated that these amendments are suitable for reclamation
of compost quality properties. These held nitrogen in compost caused
to reduce C/N ratio. Both amendments had a significant effect on
total nitrogen, but it should be used sulfuric acid in fewer amounts
(20 ml/kg fresh organic wastes); and the more amounts of acid is not
proposed.
Abstract: This paper employs a new approach to regulate the
blood glucose level of type I diabetic patient under an intensive
insulin treatment. The closed-loop control scheme incorporates
expert knowledge about treatment by using reinforcement learning
theory to maintain the normoglycemic average of 80 mg/dl and the
normal condition for free plasma insulin concentration in severe
initial state. The insulin delivery rate is obtained off-line by using Qlearning
algorithm, without requiring an explicit model of the
environment dynamics. The implementation of the insulin delivery
rate, therefore, requires simple function evaluation and minimal
online computations. Controller performance is assessed in terms of
its ability to reject the effect of meal disturbance and to overcome the
variability in the glucose-insulin dynamics from patient to patient.
Computer simulations are used to evaluate the effectiveness of the
proposed technique and to show its superiority in controlling
hyperglycemia over other existing algorithms
Abstract: Wheat gluten hydrolyzates (WGHs) and anchovy fine
powder hydrolyzates (AFPHs) were produced at 300 MPa using
combinations of Flavourzyme 500MG (F), Alcalase 2.4L (A),
Marugoto E (M) and Protamex (P), and then were compared to those
produced at ambient pressure concerning the contents of soluble solid
(SS), soluble nitrogen and electrophoretic profiles. The contents of SS
in the WGHs and AFPHs increased up to 87.2% according to the
increase in enzyme number both at high and ambient pressure. Based
on SS content, the optimum enzyme combinations for one-, two-,
three- and four-enzyme hydrolysis were determined as F, FA, FAM
and FAMP, respectively. Similar trends were found for the contents of
total soluble nitrogen (TSN) and TCA-soluble nitrogen (TCASN). The
contents of SS, TSN and TCASN in the hydrolyzates together with
electrophoretic mobility maps indicates that the high-pressure
treatment of this study accelerated protein hydrolysis compared to
ambient-pressure treatment.
Abstract: The technical realization of data transmission using
glass fiber began after the development of diode laser in year 1962.
The erbium doped fiber amplifiers (EDFA's) in high speed networks
allow information to be transmitted over longer distances without
using of signal amplification repeaters. These kinds of fibers are
doped with erbium atoms which have energy levels in its atomic
structure for amplifying light at 1550nm. When a carried signal wave
at 1550nm enters the erbium fiber, the light stimulates the excited
erbium atoms which pumped with laser beam at 980nm as additional
light. The wavelength and intensity of the semiconductor lasers
depend on the temperature of active zone and the injection current.
The present paper shows the effect of the diode lasers temperature
and injection current on the optical amplification. From the results of
in- and output power one may calculate the max. optical gain by
erbium doped fiber amplifier.
Abstract: Mycophenolic acid “MPA" is a secondary metabolite
of Penicillium bervicompactum with antibiotic and
immunosuppressive properties. In this study, fermentation process
was established for production of mycophenolic acid by Penicillium
bervicompactum MUCL 19011 in shake flask. The maximum MPA
production, product yield and productivity were 1.379 g/L, 18.6 mg/g
glucose and 4.9 mg/L.h respectively. Glucose consumption, biomass
and MPA production profiles were investigated during fermentation
time. It was found that MPA production starts approximately after
180 hours and reaches to a maximum at 280 h. In the next step, the
effects of methionine and acetate concentrations on MPA production
were evaluated. Maximum MPA production, product yield and
productivity (1.763 g/L, 23.8 mg/g glucose and 6.30 mg/L. h
respectively) were obtained with using 2.5 g/L methionine in culture
medium. Further addition of methionine had not more positive effect
on MPA production. Finally, results showed that the addition of
acetate to the culture medium had not any observable effect on MPA
production
Abstract: In an assessment of the extractability of metals in
green liquor dregs from the chemical recovery circuit of semichemical
pulp mill, extractable concentrations of heavy metals in
artificial gastric fluid were between 10 (Ni) and 717 (Zn) times
higher than those in artificial sweat fluid. Only Al (6.7 mg/kg; d.w.),
Ni (1.2 mg/kg; d.w.) and Zn (1.8 mg/kg; d.w.) showed extractability
in the artificial sweat fluid, whereas Al (730 mg/kg; d.w.), Ba (770
mg/kg; d.w.) and Zn (1290 mg/kg; d.w.) showed clear extractability
in the artificial gastric fluid. As certain heavy metals were clearly
soluble in the artificial gastric fluid, the careful handling of this
residue is recommended in order to prevent the penetration of green
liquor dregs across the human gastrointestinal tract.
Abstract: The distribution, enrichment, and accumulation of zinc
(Zn) in the sediments of Kaohsiung Ocean Disposal Site (KODS),
Taiwan were investigated. Sediment samples from two outer disposal
site stations and nine disposed stations in the KODS were collected per
quarterly in 2009 and characterized for Zn, aluminum, organic matter,
and grain size. Results showed that the mean Zn concentrations varied
from 48 mg/kg to 456 mg/kg. Results from the enrichment factor (EF)
and geo-accumulation index (Igeo) analyses imply that the sediments
collected from the KODS can be characterized between moderate and
moderately severe degree enrichment and between none and none to
medium accumulation of Zn, respectively. However, results of
potential ecological risk index indicate that the sediment has low
ecological potential risk. The EF, Igeo, and Zn concentrations at the
disposed stations were slightly higher than those at outer disposal site.
This indicated that the disposed area centers may be subjected to the
disposal impaction of harbor dredged sediments.
Abstract: The efficiency of chitosan beads processed from 4
marine animal shells; white leg shrimp (Litopenaeus vannamei), mud
crab (Scylla sp.), horseshoe crab (Carcinoscorpius rotundicauda),
and cuttlefish bone (Sepia sp.), for the adsorption experiments of
ammonia and formaldehyde were investigated. The porosities of
chitosan from the shells looked like beads were distinctly examined
under SEM. The original pores of those shells on the surface areas
compose of evenly fine pores. The shell beads of cuttlefish bone and
horseshoe crab show the larger probably even porosity, while on
those white leg shrimp and mud crab contain various large and fine
pores. The best adsorption at pH 9 in 18 mg/l ammonia at 2 hours
yield on cuttlefish bone, horseshoe crab, mud crab and white leg
shrimp with the average percent of 59.12, 51.45, 45.66 and 43.52,
respectively. Within 30 minutes the formaldehyde absorbers (at pH 5
in 8 μg/ml) revealed 46.27, 26.56, and 18.04 percent capacities in
cuttlefish bone, mud crab and white leg shrimp beads; while 22.44
percent in the horseshoe crab at pH 7. The adsorption capacities and
the amounts of beads showed a positive correlation. The adsorption
capacity relationship between pH and the gas concentrations were
affected by these qualities of chitosan beads.
Abstract: Arthrobacter viscosus biomass was used for Cr(VI)
biosorption. The effect of pH on Cr(VI) reduction and removal from
aqueous solution was studied in the range of 1-4. The Cr(VI) removal
involves both redox reaction and adsorption of metal ions on biomass
surface. The removal rate of Cr(VI) was enhanced by very acid
conditions, while higher solution pH values favored the removal of
total chromium. The best removal efficiency and uptake were
reached at pH 4, 72.5 % and 12.6 mgCr/gbiomass, respectively.
Abstract: Prolonged immobilization leads to significant
weakness and atrophy of the skeletal muscle and can also impair the
recovery of muscle strength following injury. Therefore, it is
important to minimize the period under immobilization and accelerate
the return to normal activity. This study examined the effects of heat
treatment and rest-inserted exercise on the muscle activity of the lower
limb during knee flexion/extension. Twelve healthy subjects were
assigned to 4 groups that included: (1) heat treatment + rest-inserted
exercise; (2) heat + continuous exercise; (3) no heat + rest-inserted
exercise; and (4) no heat + continuous exercise. Heat treatment was
applied for 15 mins prior to exercise. Continuous exercise groups
performed knee flexion/extension at 0.5 Hz for 300 cycles without rest
whereas rest-inserted exercise groups performed the same exercise but
with 2 mins rest inserted every 60 cycles of continuous exercise.
Changes in the rectus femoris and hamstring muscle activities were
assessed at 0, 1, and 2 weeks of treatment by measuring the
electromyography signals of isokinetic maximum voluntary
contraction. Significant increases in both the rectus femoris and
hamstring muscles were observed after 2 weeks of treatment only
when both heat treatment and rest-inserted exercise were performed.
These results suggest that combination of various treatment techniques,
such as heat treatment and rest-inserted exercise, may expedite the
recovery of muscle strength following immobilization.
Abstract: Soil microbial activity is adversely affected by pollutants such as heavy metals, antibiotics and pesticides. Organic amendments including sewage sludge, municipal compost and vermicompost are recently used to improve soil structure and fertility. But, these materials contain heavy metals including Pb, Cd, Zn, Ni and Cu that are toxic to soil microorganisms and may lead to occurrence of more tolerant microbes. Among these, Pb is the most abundant and has more negative effect on soil microbial ecology. In this study, Pb levels of 0, 100, 200, 300, 400 and 500 mg Pb [as Pb(NO3)2] per kg soil were added to the pots containing 2 kg of a loamy soil and incubated for 6 months at 25°C with soil moisture of - 0.3 MPa. Dehydrogenase activity of soil as a measure of microbial activity was determined on 15, 30, 90 and 180 days after incubation. Triphenyl tetrazolium chloride (TTC) was used as an electron acceptor in this assay. PICTs (IC50 values) were calculated for each Pb level and incubation time. Soil microbial activity was decreased by increasing Pb level during 30 days of incubation but the induced tolerance appeared on day 90 and thereafter. During 90 to 180 days of incubation, the PICT was gradually developed by increasing Pb level up to 200 mg kg-1, but the rate of enhancement was steeper at higher concentrations.