Abstract: The methanolic extracts from seeds of tamarind
(Tamarindus indica) was prepared by Soxhlet apparatus extraction
and evaluated for total phenolic content by Folin-Ciocalteu method.
Then, methanolic extract was screened biological activities (In vitro)
for anti-melanogenic activity by tyrosinase inhibition test, antiinflammation
activity by cyclooxygenase 1 (COX-1) and
cyclooxygenase 2 (COX-2) inhibition test, and cytotoxic screening
test with Vero cells. The results showed that total phenolic content,
which contained in extract, was contained 27.72 mg of gallic acid
equivalent per g of dry weight. The ability to inhibit tyrosinase
enzyme, which exerted by Tamarind seed extracts (1 mg/ml) was
52.13 ± 0.42 %. The extract was not possessed inhibitory effect to
COX-1 and COX-2 enzymes and cytotoxic effect to Vero cells. The
finding is concludes that tested seed extract was possessed
antimelanogenic activity with non-toxic effects. However, there was
not exhibited anti-inflammatory activity. Further studies include the
use of advance biological models to confirm this biological activity,
as well as, the isolation and characterization of the purified
compounds that it was contained.
Abstract: Electromyography (EMG) signal processing has been investigated remarkably regarding various applications such as in rehabilitation systems. Specifically, wavelet transform has served as a powerful technique to scrutinize EMG signals since wavelet transform is consistent with the nature of EMG as a non-stationary signal. In this paper, the efficiency of wavelet transform in surface EMG feature extraction is investigated from four levels of wavelet decomposition and a comparative study between different mother wavelets had been done. To recognize the best function and level of wavelet analysis, two evaluation criteria, scatter plot and RES index are recruited. Hereupon, four wavelet families, namely, Daubechies, Coiflets, Symlets and Biorthogonal are studied in wavelet decomposition stage. Consequently, the results show that only features from first and second level of wavelet decomposition yields good performance and some functions of various wavelet families can lead to an improvement in separability class of different hand movements.
Abstract: The present work represents an investigation of the
hydrolysis of hull-less pumpkin (Cucurbita Pepo L.) oil cake protein
isolate (PuOC PI) by pepsin. To examine the effectiveness and
suitability of pepsin towards PuOC PI the kinetic parameters for
pepsin on PuOC PI were determined and then, the hydrolysis process
was studied using Response Surface Methodology (RSM). The
hydrolysis was carried out at temperature of 30°C and pH 3.00. Time
and initial enzyme/substrate ratio (E/S) at three levels were selected
as the independent parameters. The degree of hydrolysis, DH, was
mesuared after 20, 30 and 40 minutes, at initial E/S of 0.7, 1 and 1.3
mA/mg proteins. Since the proposed second-order polynomial model
showed good fit with the experimental data (R2 = 0.9822), the
obtained mathematical model could be used for monitoring the
hydrolysis of PuOC PI by pepsin, under studied experimental
conditions, varying the time and initial E/S. To achieve the highest
value of DH (39.13 %), the obtained optimum conditions for time
and initial E/S were 30 min and 1.024 mA/mg proteins.
Abstract: For stricter drinking water regulations in the future, reducing the humic acid and disinfection byproducts in raw water, namely, trihalomethanes (THMs) and haloacetic acids (HAAs) is worthy for research. To investigate the removal of waterborne organic material using a lab-scale of bio-activated carbon filter under different EBCT, the concentrations of humic acid prepared were 0.01, 0.03, 0.06, 0.12, 0.17, 0.23, and 0.29 mg/L. Then we conducted experiments using a pilot plant with in-field of the serially connected bio-activated carbon filters and hollow fiber membrane processes employed in traditional water purification plants. Results showed under low TOC conditions of humic acid in influent (0.69 to 1.03 mg TOC/L) with an EBCT of 30 min, 40 min, and 50 min, TOC removal rates increases with greater EBCT, attaining about 39 % removal rate. The removal rate of THMs and HAAs by BACF was 54.8 % and 89.0 %, respectively.
Abstract: An experiment was conducted using two aeration
methods (water-into-air and air-into-water) and followed by filtration
processes using manganese greensand material. The properties of
groundwater such as pH, dissolved oxygen, turbidity and heavy metal
concentration (iron and manganese) will be assessed. The objectives
of this study are i) to determine the effective aeration method and ii)
to assess the effectiveness of manganese greensand as filter media in
removing iron and manganese concentration in groundwater. Results
showed that final pH for all samples after treatment are in range from
7.40 and 8.40. Both aeration methods increased the dissolved oxygen
content. Final turbidity for groundwater samples are between 3 NTU
to 29 NTU. Only three out of eight samples achieved iron
concentration of 0.3mg/L and less and all samples reach manganese
concentration of 0.1mg/L and less. Air-into-water aeration method
gives higher percentage of iron and manganese removal compare to
water-into-air method.
Abstract: In this study, the ability of Aspergillus niger and
Penicillium simplicissimum to extract heavy metals from a spent
refinery catalyst was investigated. For the first step, a spent
processing catalyst from one of the oil refineries in Iran was
physically and chemically characterized. Aspergillus niger and
Penicillium simplicissimum were used to mobilize Al/Co/Mo/Ni from
hazardous spent catalysts. The fungi were adapted to the mixture of
metals at 100-800 mg L-1 with increments in concentration of 100 mg
L-1. Bioleaching experiments were carried out in batch cultures. To
investigate the production of organic acids in sucrose medium,
analyses of the culture medium by HPLC were performed at specific
time intervals after inoculation. The results obtained from Inductive
coupled plasma-optical emission spectrometry (ICP-OES) showed
that after the one-step bioleaching process using Aspergillus niger,
maximum removal efficiencies of 27%, 66%, 62% and 38% were
achieved for Al, Co, Mo and Ni, respectively. However, the highest
removal efficiencies using Penicillium simplicissimum were of 32%,
67%, 65% and 38% for Al, Co, Mo and Ni, respectively
Abstract: The direct discharge of palm oil mill effluent (POME) wastewater causes serious environmental pollution due to its high chemical oxygen demand (COD) and biochemical oxygen demand (BOD). Traditional ways for POME treatment have both economical and environmental disadvantages. In this study, a membrane anaerobic system (MAS) was used as an alternative, cost effective method for treating POME. Six steady states were attained as a part of a kinetic study that considered concentration ranges of 8,220 to 15,400 mg/l for mixed liquor suspended solids (MLSS) and 6,329 to 13,244 mg/l for mixed liquor volatile suspended solids (MLVSS). Kinetic equations from Monod, Contois and Chen & Hashimoto were employed to describe the kinetics of POME treatment at organic loading rates ranging from 2 to 13 kg COD/m3/d. throughout the experiment, the removal efficiency of COD was from 94.8 to 96.5% with hydraulic retention time, HRT from 400.6 to 5.7 days. The growth yield coefficient, Y was found to be 0.62gVSS/g COD the specific microorganism decay rate was 0.21 d-1 and the methane gas yield production rate was between 0.25 l/g COD/d and 0.58 l/g COD/d. Steady state influent COD concentrations increased from 18,302 mg/l in the first steady state to 43,500 mg/l in the sixth steady state. The minimum solids retention time, which was obtained from the three kinetic models ranged from 5 to 12.3 days. The k values were in the range of 0.35 – 0.519 g COD/ g VSS • d and values were between 0.26 and 0.379 d-1. The solids retention time (SRT) decreased from 800 days to 11.6 days. The complete treatment reduced the COD content to 2279 mg/l equivalent to a reduction of 94.8% reduction from the original.
Abstract: Biological treatment of secondary effluent wastewater
by two combined denitrification/oxic filtration systems packed with
Lock type(denitrification filter) and ceramic ball (oxic filter) has been
studied for 5months. Two phases of operating conditions were carried
out with an influent nitrate and ammonia concentrations varied from
5.8 to 11.7mg/L and 5.4 to 12.4mg/L,respectively.
Denitrification/oxic filter treatment system were operated under an
EBCT (Empty Bed Contact Time) of 4h at system recirculation ratio in
the range from 0 to 300% (Linear Velocity increased 19.5m/d to
78m/d). The system efficiency of denitrification , nitrification over
95% respectively. Total nitrogen and COD removal range from
54.6%(recirculation 0%) to 92.3%(recirculation 300%) and 10% to
62.5%, respectively.
Abstract: Removal of PCP by a system combining
biodegradation by biofilm and adsorption was investigated here.
Three studies were conducted employing batch tests, sequencing
batch reactor (SBR) and continuous biofilm activated carbon
column reactor (BACCOR). The combination of biofilm-GAC
batch process removed about 30% more PCP than GAC adsorption
alone. For the SBR processes, both the suspended and attached
biomass could remove more than 90% of the PCP after
acclimatisation. BACCOR was able to remove more than 98% of
PCP-Na at concentrations ranging from 10 to 100 mg/L, at empty
bed contact time (EBCT) ranging from 0.75 to 4 hours. Pure and
mixed cultures from BACCOR were tested for use of PCP as sole
carbon and energy source under aerobic conditions. The isolates
were able to degrade up to 42% of PCP under aerobic conditions in
pure cultures. However, mixed cultures were found able to degrade
more than 99% PCP indicating interdependence of species.
Abstract: Engineered nanoparticles’ usage rapidly increased in
various applications in the last decade due to their unusual properties.
However, there is an ever increasing concern to understand their
toxicological effect in human health. Particularly, metal and metal
oxide nanoparticles have been used in various sectors including
biomedical, food and agriculture. But their impact on human health is
yet to be fully understood. In this present investigation, we assessed
the toxic effect of engineered nanoparticles (ENPs) including Ag,
MgO and Co3O4 nanoparticles (NPs) on human mesenchymal stem
cells (hMSC) adopting cell viability and cellular morphological
changes as tools The results suggested that silver NPs are more toxic
than MgO and Co3O4NPs. The ENPs induced cytotoxicity and
nuclear morphological changes in hMSC depending on dose. The cell
viability decreases with increase in concentration of ENPs. The
cellular morphology studies revealed that ENPs damaged the cells.
These preliminary findings have implications for the use of these
nanoparticles in food industry with systematic regulations.
Abstract: The purpose of this research is to establish the experimental conditions for removal of Cibacron Brilliant Yellow 3G-P dye (CBY) from aqueous solutions by sorption onto coffee husks as a low-cost sorbent. The effects of various experimental parameters (e.g. initial CBY dye concentration, sorbent mass, pH, temperature) were examined and the optimal experimental conditions were determined. The results indicated that the removal of the dye was pH dependent and at initial pH of 2, the dye was removed effectively. The CBY dye sorption data were fitted to Langmuir, Freundlich, Temkin and Dubinin-Radushkevich equilibrium models. The maximum sorption capacity of CBY dye ions onto coffee husks increased from 24.04 to 35.04 mg g-1 when the temperature was increased from 293 to 313 K. The calculated sorption thermodynamic parameters including ΔG°, ΔH°, and ΔS° indicated that the CBY dye sorption onto coffee husks is a spontaneous, endothermic and mainly physical in nature.
Abstract: SEMG (Surface Electromyogram) is one of the
bio-signals and is generated from the muscle. And there are many
research results that use forearm EMG to detect hand motions. In this
paper, we will talk about our developed the robot hand system that can
control grasping power by SEMG. In our system, we suppose that
muscle power is proportional to the amplitude of SEMG. The power is
estimated and the grip power of a robot hand is able to be controlled
using estimated muscle power in our system. In addition, to perform a
more precise control can be considered to build a closed loop feedback
system as an object to a subject to pressure from the edge of hand. Our
objectives of this study are the development of a method that makes
perfect detection of the hand grip force possible using SEMG patterns,
and applying this method to the man-machine interface.
Abstract: Simultaneous recovery of copper and DCA from
simulated MEUF concentrated stream was investigated. Effects of
surfactant (DCA) and metal (copper) concentrations, surfactant to
metal molar ratio (S/M ratio), electroplating voltage, EDTA
concentration, solution pH, and salt concentration on metal recovery
and current efficiency were studied. Electric voltage of -0.5 V was
shown to be optimum operation condition in terms of Cu recovery,
current efficiency, and surfactant recovery. Increasing Cu recovery and
current efficiency were observed with increases of Cu concentration
while keeping concentration of DCA constant. However, increasing
both Cu and DCA concentration while keeping S/M ratio constant at
2.5 showed detrimental effect on Cu recovery at DCA concentration
higher than 15 mM. Cu recovery decreases with increasing pH while
current efficiency showed an opposite trend. It is believed that
conductivity is the main cause for discrepancy of Cu recovery and
current efficiency observed at different pH. Finally, it was shown that
EDTA had adverse effect on both Cu recovery and current efficiency
while addition of NaCl salt had negative impact on current efficiency
at concentration higher than 8000 mg/L.
Abstract: In this paper we have proposed a methodology to
develop an amperometric biosensor for the analysis of glucose
concentration using a simple microcontroller based data acquisition
system. The work involves the development of Detachable
Membrane Unit (enzyme based biomembrane) with immobilized
glucose oxidase on the membrane and interfacing the same to the
signal conditioning system. The current generated by the biosensor
for different glucose concentrations was signal conditioned, then
acquired and computed by a simple AT89C51-microcontroller. The
optimum operating parameters for the better performance were found
and reported. The detailed performance evaluation of the biosensor
has been carried out. The proposed microcontroller based biosensor
system has the sensitivity of 0.04V/g/dl, with a resolution of
50mg/dl. It has exhibited very good inter day stability observed up to
30 days. Comparing to the reference method such as HPLC, the
accuracy of the proposed biosensor system is well within ± 1.5%.
The system can be used for real time analysis of glucose
concentration in the field such as, food and fermentation and clinical
(In-Vitro) applications.
Abstract: UML is a collection of notations for capturing a software system specification. These notations have a specific syntax defined by the Object Management Group (OMG), but many of their constructs only present informal semantics. They are primarily graphical, with textual annotation. The inadequacies of standard UML as a vehicle for complete specification and implementation of real-time embedded systems has led to a variety of competing and complementary proposals. The Real-time UML profile (UML-RT), developed and standardized by OMG, defines a unified framework to express the time, scheduling and performance aspects of a system. We present in this paper a framework approach aimed at deriving a complete specification of a real-time system. Therefore, we combine two methods, a semiformal one, UML-RT, which allows the visual modeling of a realtime system and a formal one, CSP+T, which is a design language including the specification of real-time requirements. As to show the applicability of the approach, a correct design of a real-time system with hard real time constraints by applying a set of mapping rules is obtained.
Abstract: Mature landfill leachates contain some macromolecular organic substances that are resistant to biological degradation. Recently, Fenton-s oxidation has been investigated for chemical treatment or pre-treatment of mature landfill leachates. The aim of this study was to reduce the recalcitrant organic load still remaining after the complete treatment of a mature landfill leachate by Fenton-s oxidation post-treatment. The effect of various parameters such as H2O2 to Fe2+ molar ratio, dosage of Fe2+ reagent, initial pH, reaction time and initial chemical oxygen demand (COD) strength, that have an important role on the oxidation, was analysed. A molar ratio H2O2/Fe2+ = 3, a Fe2+ dosage of 4 mmol·L-1, pH 3, and a reaction time of 40 min were found to achieve better oxidation performances. At these favorable conditions, COD removal efficiency was 60.9% and 31.1% for initial COD of 93 and 743 mg·L-1 respectively (diluted and non diluted leachate). Fenton-s oxidation also presented good results for color removal. In spite of being extremely difficult to treat this leachate, the above results seem rather encouraging on the application of Fenton-s oxidation.
Abstract: Rice bran has been abandoned as agricultural waste for million tonnes per year in Thailand, therefore they have been proposed to be utilized as a rich carbon source in the production of bioethanol. Many toxic compounds are possibly released during the pretreatment of rice bran prior the fermentation process. This study aims to analyze on the availability of toxic compounds and the amount of glucose obtained from 2 different pretreatments using sulfuric acid and mixed cellulase enzymes (without and with delignification/ activated charcoal). The concentration of furfural, 5- hydroxymethyl furfural (5-HMF), levulinic acid, vanillin, syringaldehyde and4-hydroxybenzaldehyde (4-HB) and the percent acetic acid were found to be 0.0517 ± 0.049 mg/L, 0.032 ± 0.06 mg/L, 21074 ± 1685.62 mg/L, 126.265 ± 6.005 mg/L, 2.89 ± 0.30 mg/L, 0.37 ± 0.031mg/L and 0.72% under the pretreatment process without delignification/ activated charcoal treatment and 384.47 ± 99.02 g/L, 0.068 mg/L, 142107.62 ± 8664.6 mg/L, 0.19 mg/L, 5.43 ± 3.29 mg/L, 4.80 ± 0.76 mg/L and 0.254% under the pretreatment process with delignification/ activated charcoal treatment respectively. The presence of high concentration of acetic acid was found to impede the growth of Zymomonas mobilis strain TISTR 551 despite the present of high concentration of levulinic acid. Z. mobilis strain TISTR 551 was found to produce 8.96 ± 4.06 g/L of ethanol under 4 days fementation period in biofilm stage in which represented 40% theoretical yield.
Abstract: Feature and model selection are in the center of
attention of many researches because of their impact on classifiers-
performance. Both selections are usually performed separately but
recent developments suggest using a combined GA-SVM approach to
perform them simultaneously. This approach improves the
performance of the classifier identifying the best subset of variables
and the optimal parameters- values. Although GA-SVM is an
effective method it is computationally expensive, thus a rough
method can be considered. The paper investigates a joined approach
of Genetic Algorithm and kernel matrix criteria to perform
simultaneously feature and model selection for SVM classification
problem. The purpose of this research is to improve the classification
performance of SVM through an efficient approach, the Kernel
Matrix Genetic Algorithm method (KMGA).
Abstract: Lung cancer accounts for the most cancer related deaths for men as well as for women. The identification of cancer associated genes and the related pathways are essential to provide an important possibility in the prevention of many types of cancer. In this work two filter approaches, namely the information gain and the biomarker identifier (BMI) are used for the identification of different types of small-cell and non-small-cell lung cancer. A new method to determine the BMI thresholds is proposed to prioritize genes (i.e., primary, secondary and tertiary) using a k-means clustering approach. Sets of key genes were identified that can be found in several pathways. It turned out that the modified BMI is well suited for microarray data and therefore BMI is proposed as a powerful tool for the search for new and so far undiscovered genes related to cancer.
Abstract: The reservoir of Kowsar dam supply water for
different usages such as aquaculture farms , drinking, agricultural and
industrial usages for some provinces in south of Iran. The Kowsar
dam is located next to the city of Dehdashat in Kohgiluye and
Boyerahmad province in southern Iran. There are some towns and
villages on the Kowsar dam watersheds, which Dehdasht and Choram
are the most important and populated twons in this area, which can to
be sources of pollution for water reservoir of the Kowsar dam . This
study was done to determine of water pollution of the Kowsar dam
reservoir which is one of the most important water resources of
Kohkiloye and Boyerahmad and Bushehr provinces in south-west
Iran. In this study , water samples during 12 months were collected to
examine Biochemical Oxygen Demand (BOD) and Dissolved
Oxygen(DO) as a criterion for evaluation of water pollution of the
reservoir. In summary ,the study has shown Maximum, average and
minimum levels of BOD have observed 25.9 ,9.15 and 2.3 mg/L
respectively and statistical parameters of data such as standard
deviation , variance and skewness have calculated 7.88, 62 and 1.54
respectively. Finally the results were compared with Iranian national
standards. Among the analyzed samples, as the maximum value of
BOD (25.9 mg/L) was observed at the May 2010 , was within the
maximum admissible limits by the Iranian standards.