Abstract: Titanium alloys like Ti-6Al-2Sn-4Zr-6Mo (Ti-
6246) are widely used in aerospace applications. Component
manufacturing, however, is difficult and expensive as their
machinability is extremely poor. A thorough understanding of the
chip formation process is needed to improve related metal cutting
operations.In the current study, orthogonal cutting experiments have
been performed and theresulting chips were analyzed by optical
microscopy and scanning electron microscopy.Chips from aTi-
6246ingot were produced at different cutting speeds and cutting
depths. During the experiments, depending of the cutting conditions,
continuous or segmented chips were formed. Narrow, highly
deformed and grain oriented zones, the so-called shear zone,
separated individual segments. Different material properties have
been measured in the shear zones and the segments.
Abstract: In this work we investigated the behavior of methane
hydrates dispersed in crude oils from different fields at temperatures
below 0°C. In case of crude oil emulsion the size of water droplets is
in the range of 50e100"m. The size of hydrate particles formed from
droplets is the same. The self-preservation is not expected in this
field. However, the self-preservation of hydrates with the size of
particles 24±18"m (electron microscopy data) in suspensions is
observed. Similar results were obtained for four different kinds of
crude oil and model system such as asphaltenes, resins and wax in ndecane.
This result can allow developing effective methods to prevent
the formation and elimination of gas-hydrate plugs in pipelines under
low temperature conditions (e. g. in Eastern Siberia). There is a
prospective to use experiment results for working out the technology
of associated petroleum gas recovery.
Abstract: Langmuir–Blodgett (LB) films of polyaniline (PANI) grown onto ITO coated glass substrates were utilized for the fabrication of Uric acid biosensor for efficient detection of uric acid by immobilizing Uricase via EDC–NHS coupling. The modified electrodes were characterized by atomic force microscopy (AFM). The response characteristics after immobilization of uricase were studied using cyclic voltammetry and electrochemical impedance spectroscopy techniques. The uricase/PANI/ITO/glass bioelectrode studied by CV and EIS techniques revealed detection of uric acid in a wide range of 0.05 mM to 1.0 mM, covering the physiological range in blood. A low Michaelis–Menten constant (Km) of 0.21 mM indicates the higher affinity of immobilized Uricase towards its analyte (uric acid). The fabricated uric acid biosensor based on PANI LB films exhibits excellent sensitivity of 0.21 mA/mM with a response time of 4 s, good reproducibility, long shelf life (8 weeks) and high selectivity.
Abstract: The gustatory system allows animals to distinguish
varieties of food and affects greatly the consumption of food, hence
the health and growth of animals. In the current study, we
investigated the histogenesis of vallate papillae (VLP) in the rabbit
tongue using light and scanning electron microscopy. Samples were
obtained from rabbit embryos at the embryonic days 16-30 (E16-30),
and from newborns until maturity; 6 months. At E16, the first
primordia of vallate papillae were observed as small pits on the
surface epithelium of the tongue-s root. At E18, the caudal part was
prominent with loose mesenchymal tissue core; meanwhile the rostral
part of the papilla was remained as a thick mass of epithelial cells. At
E20-24, the side epithelium formed the primitive annular groove. At
E26, the primitive taste buds appeared only at the papillary surface
and reached their maturity by E28. The annular groove started to
appear at E26 became more defined at E28. The definitive vallate
papillae with substantial number of apparently mature taste buds
were observed by the end of the second week. We conclude that the
vallate papillae develop early and mature during the early postnatal
life.
Abstract: Toxoplasma gondii is an intracellular parasite capable
of infecting all nucleated cells in a diverse array of species.
Toxoplasma plaque assay have been described using Bacto Agar.
Because of its experimental advantages carboxymethyl cellulose
overlay, medium viscosity was choosing and the aim of this work
was to develop alternative method for formation of T. gondii plaques.
Tachyzoites were inoculated onto monolayers of Vero cells and
cultured at 37° C under 5 % CO2. The cultures were followed up by
microscopy inspection. Small plaques were visible by naphtol blue
stain 4 days after infection. Larger plaques could be observed by day
10 of culture. The carboxymethyl cellulose is a cheap reagent and the
methodology is easier, faster than assays under agar overlay. This is
the first description of the carboxymethyl cellulose overlay use for
obtaining the formation of T. gondii plaques and may be useful in
consequent obtaining tachyzoites for detailed studies.
Abstract: A biosensor based on glucose oxidase (GOx) immobilized onto nanoparticles zirconium oxide with polyethylene nanocomposite for glucose monitoring has been designed. The CTAB/PEG/ZrO2/GOx nanocomposite was deposited onto screen printed carbon paste (SPCE) electrode via spin coating technique. The properties of CTAB/PEG/ZrO2/GOx were study using scanning electron microscopy (SEM). The SPE modified with the CTAB/PEG/ZrO2/GOx showed electrocatalytical response to the oxidation of glucose when ferrocene carboxaldehyde was used as an artificial redox mediator, which was studied by cyclic voltammetry (CV). Several parameters such as working potential, effect of pH and effect of ZrO2/PEG layers that governed the analytical performance of the biosensor, have been studied. The biosensor was applied to detect glucose with a linear range of 0.4 to 2.0 mmol L−1 with good repetability and reproducibility.
Abstract: Antimicrobial (AM) starch-based films were
developed by incorporating chitosan and lauric acid as antimicrobial
agent into starch-based film. Chitosan has wide range of applications
as a biomaterial, but barriers still exist to its broader use due to its
physical and chemical limitations. In this work, a series of
starch/chitosan (SC) blend films containing 8% of lauric acid was
prepared by casting method. The structure of the film was
characterized by Fourier transform infrared spectroscopy (FTIR), Xray
diffraction (XRD), and scanning electron microscopy (SEM).
The results indicated that there were strong interactions were present
between the hydroxyl groups of starch and the amino groups of
chitosan resulting in a good miscibility between starch and chitosan
in the blend films. Physical properties and optical properties of the
AM starch-based film were evaluated. The AM starch-based films
incorporated with chitosan and lauric acid showed an improvement in
water vapour transmission rate (WVTR) and addition of starch
content provided more transparent films while the yellowness of the
film attributed to the higher chitosan content. The improvement in
water barrier properties was mainly attributed to the hydrophobicity
of lauric acid and optimum chitosan or starch content. AM starch
based film also showed excellent oxygen barrier. Obtaining films
with good oxygen permeability would be an indication of the
potential use of these antimicrobial packaging as a natural packaging
and an alternative packaging to the synthetic polymer to protect food
from oxidation reactions
Abstract: In order to avoid the potentially devastating
consequences of global warming and climate change, the carbon
dioxide “CO2" emissions caused due to anthropogenic activities must
be reduced considerably. This paper presents the first study
examining the feasibility of carbon sequestration in construction and
demolition “C&D" waste. Experiments were carried out in a self
fabricated Batch Reactor at 40ºC, relative humidity of 50-70%, and
flow rate of CO2 at 10L/min for 1 hour for water-to-solids ratio of 0.2
to 1.2. The effect of surface area was found by comparing the
theoretical extent of carbonation of two different sieve sizes (0.3mm
and 2.36mm) of C&D waste. A 38.44% of the theoretical extent of
carbonation equating to 4% CO2 sequestration extent was obtained
for C&D waste sample for 0.3mm sieve size. Qualitative,
quantitative and morphological analyses were done to validate
carbonate formation using X-ray diffraction “X.R.D.," thermal
gravimetric analysis “T.G.A., “X-Ray Fluorescence Spectroscopy
“X.R.F.," and scanning electron microscopy “S.E.M".
Abstract: A simple approach is demonstrated for growing large
scale, nearly vertically aligned ZnO nanowire arrays by thermal
oxidation method. To reveal effect of temperature on growth and
physical properties of the ZnO nanowires, gold coated zinc substrates
were annealed at 300 °C and 400 °C for 4 hours duration in air. Xray
diffraction patterns of annealed samples indicated a set of well
defined diffraction peaks, indexed to the wurtzite hexagonal phase of
ZnO. The scanning electron microscopy studies show formation of
ZnO nanowires having length of several microns and average of
diameter less than 500 nm. It is found that the areal density of wires
is relatively higher, when the annealing is carried out at higher
temperature i.e. at 400°C. From the field emission studies, the values
of the turn-on and threshold field, required to draw emission current
density of 10 μA/cm2 and 100 μA/cm2 are observed to be 1.2 V/μm
and 1.7 V/μm for the samples annealed at 300 °C and 2.9 V/μm and
3.7 V/μm for that annealed at 400 °C, respectively. The field
emission current stability, investigated over duration of more than 2
hours at the preset value of 1 μA, is found to be fairly good in both
cases. The simplicity of the synthesis route coupled with the
promising field emission properties offer unprecedented advantage
for the use of ZnO field emitters for high current density
applications.
Abstract: Chemical vapor deposition method was used to
produce carbon nanotubes on an iron based catalyst from acetylene.
Gas-phase samples collected from the different positions of the tubular reactor were analyzed by GC/MS. A variety of species ranging from hydrogen to naphthalene were observed and changes in their concentrations were plotted against the reactor position. Briefly benzene, toluene, styrene, indene and naphthalene were the main
higher molecular weight species and vinylacetylene and diacetylene were the important intermediates. Nanotube characterization was performed by scanning electron microscopy and transmission electron microscopy.
Abstract: Platinum oxide nanoparticles were prepared by a
simple hydrothermal route and chemical reduction using
carbohydrates (Fructose and sucrose) as the reducing and
stabilizing agents. The crystallite size of these nanoparticles was
evaluated from X-ray diffraction (XRD), atomic force microscopy
(AFM) and transmission electron microscopy (TEM) and was
found to be 10 nm as shown in figure 1, which is the
demonstration of EM bright field and transmission electron
microscopy. The effect of carbohydrates on the morphology of the
nanoparticles was studied using TEM (Figure 1). The
nanoparticles (100 μg/ml) were administered to the Pseudomonas
Stutzeri and Lactobacillus cultures and the incubation was done at
35 oC for 24 hours. The nanocomposites exhibited interesting
inhibitory as well as bactericidal activity against P. Stutzeri and
and Lactobacillus species. Incorporation of nanoparticles also
increased the thermal stability of the carbohydrates.
Abstract: Nanowire arrays of copper with uniform diameters have
been synthesized by potentiostatic electrochemical metal deposition
(EMD) of copper sulphate and potassium chloride solution within
the nano-channels of porous Indium-Tin Oxide (ITO), also known as
Tin doped Indium Oxide templates. The nanowires developed were
fairly continuous with diameters ranging from 110-140 nm along
the entire length. Single as well as poly-crystalline copper wires
have been prepared by application of appropriate potential during the
EMD process. Scanning electron microscopy (SEM), high resolution
transmission electron microscopy (HRTEM), small angle electron
diffraction (SAED) and atomic force microscopy (AFM) were used
to characterize the synthesized nano wires at room temperature. The
electrochemical response of synthesized products was evaluated by
cyclic voltammetry while surface energy analysis was carried out
using a Goniometer.
Abstract: This paper presents the doping profile measurement
and characterization technique for the pocket implanted nano scale
n-MOSFET. Scanning capacitance microscopy and atomic force
microscopy have been used to image the extent of lateral dopant
diffusion in MOS structures. The data are capacitance vs. voltage
measurements made on a nano scale device. The technique is nondestructive
when imaging uncleaved samples. Experimental data from
the published literature are presented here on actual, cleaved device
structures which clearly indicate the two-dimensional dopant profile
in terms of a spatially varying modulated capacitance signal. Firstorder
deconvolution indicates the technique has much promise for
the quantitative characterization of lateral dopant profiles. The pocket
profile is modeled assuming the linear pocket profiles at the source
and drain edges. From the model, the effective doping concentration
is found to use in modeling and simulation results of the various
parameters of the pocket implanted nano scale n-MOSFET. The
potential of the technique to characterize important device related
phenomena on a local scale is also discussed.
Abstract: This paper deals with the evaluation of flow properties
of polymeric matrix with natural animal fillers. Technical university
of Liberec cooperates on the long-term development of “green
materials“ that should replace conventionally used materials
(especially in automotive industry). Natural fibres (of animal and
plant origin) from all over the world are collected and adapted
(drying, cutting etc.) for extrusion processing. Inside the extruder
these natural additives are blended with polymeric (synthetic and
biodegradable - PLA) matrix and created compound is subsequently
cut for pellets in the wet way. These green materials with unique
recipes are then studied and their mechanical, physical and
processing properties are determined. The main goal of this research
is to develop new ecological materials very similar to unfilled
polymers. In this article the rheological behaviour of chosen natural
animal fibres is introduced considering their shape and surface that
were observed with use of SEM microscopy.
Abstract: Among the many promising nanomaterials with antifungal properties, metal nanoparticles (silver nanoparticles) stand out due to their high chemical activity. Therefore, the aim of this study was to evaluate the effect of silver nanoparticles (AgNPs) against Phomopsis sp. AgNPs were synthesized by silver nitrate reduction with sodium citrate and stabilized with ammonia. The synthesized AgNPs have further been characterized by UV/Visible spectroscopy, Biophysical techniques like Dynamic light scattering (DLS) and Scanning Electron Microscopy (SEM). The average diameter of the prepared silver colloidal nanoparticles was about 52 nm. Absolute inhibitions (100%) were observed on treated with a 270 and 540 µg ml-1 concentration of AgNPs. The results from the study of the AgNPs antifungal effect are significant and suggest that the synthesized silver nanoparticles may have an advantage compared with conventional fungicides.
Abstract: Silver/polylactide nanocomposites (Ag/PLA-NCs) were
synthesized via chemical reduction method in diphase solvent. Silver
nitrate and sodium borohydride were used as a silver precursor
and reducing agent in the polylactide (PLA). The properties of
Ag/PLA-NCs were studied as a function of the weight percentages
of silver nanoparticles (8, 16 and 32 wt% of Ag-NPs) relative to
the weight of PLA. The Ag/PLA-NCs were characterized by Xray
diffraction (XRD), transmission electron microscopy (TEM),
electro-optical microscopy (EOM), UV-visible spectroscopy (UV-vis)
and Fourier transform infrared spectroscopy (FT-IR). XRD patterns
confirmed that Ag-NPs crystallographic planes were face centered
cubic (fcc) type. TEM images showed that mean diameters of Ag-NPs
were 3.30, 3.80 and 4.80 nm. Electro-optical microscopy revealed
excellent dispersion and interaction between Ag-NPs and PLA films.
The generation of silver nanoparticles was confirmed from the UVvisible
spectra. FT-IR spectra showed that there were no significant
differences between PLA and Ag/PLA-NCs films. The synthesized
Ag/PLA-NCs were stable in organic solution over a long period of
time without sign of precipitation.
Abstract: Hydrogen is considered to be the most promising
candidate as a future energy carrier. One of the most used
technologies for the electrolytic hydrogen production is alkaline
water electrolysis. However, due to the high energy requirements, the
cost of hydrogen produced in such a way is high. In continuous
search to improve this process using advanced electrocatalytic
materials for the hydrogen evolution reaction (HER), Ni type Raney
and macro-porous Ni-Co electrodes were prepared on AISI 304
stainless steel substrates by electrodeposition. The developed
electrodes were characterized by SEM and confocal laser scanning
microscopy. HER on these electrodes was evaluated in 30 wt.% KOH
solution by means of hydrogen discharge curves and galvanostatic
tests. Results show that the developed electrodes present a most
efficient behaviour for HER when comparing with the smooth Ni
cathode. It has been reported a reduction in the energy consumption
of the electrolysis cell of about 25% by using the developed coatings
as cathodes.
Abstract: A novel physico-chemical route to produce few layer graphene nanoribbons with atomically smooth edges is reported, via acid treatment (H2SO4:HNO3) followed by characteristic thermal shock processes involving extremely cold substances. Samples were studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Raman spectroscopy and X-ray photoelectron spectroscopy. This method demonstrates the importance of having the nanotubes open ended for an efficient uniform unzipping along the nanotube axis. The average dimensions of these nanoribbons are approximately ca. 210 nm wide and consist of few layers, as observed by transmission electron microscopy. The produced nanoribbons exhibit different chiralities, as observed by high resolution transmission electron microscopy. This method is able to provide graphene nanoribbons with atomically smooth edges which could be used in various applications including sensors, gas adsorption materials, composite fillers, among others.
Abstract: This paper investigates the influence of various
parameters on the behaviour of water droplets on polymeric surfaces
under high electric fields. An inclined plane test was carried out to
understand the droplet behaviour in strong electric field. Parameters
such as water droplet conductivity, droplet volume, polymeric
surface roughness and droplet positioning with respect to the
electrodes were studied. The flashover voltage is affected by all
aforementioned parameters. The droplet positioning is in some cases
more vital than the droplet volume. Surface damages were analysed
using Scanning Electron Microscopy (SEM) studies and by Energy
dispersive X-ray Analysis (EDAX). It is observes that magnitude of
discharge have direct influence on amount of surface da
Abstract: Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color" channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes.