Vetiver Oil Production from Root Culture of Vetiveria zizanioides
Vetiver oil is secondary metabolite that accumulates in Vetiveria zizanioides roots. The aim of this study was to obtain best type of root culture which produce high amount of vetiver oil, and was similar to metabolites produce from its mother plant. Protein analysis was also conducted to detect protein, related to putative enzymes, which have a role in terpenoids synthesis in the root culture. The results showed that root culture derived from crown explant produced the best root growth. The root culture produced primary and lateral roots, ca. 40 branches. The vetiver oil produced from root culture was analyzed by using GC-MS., and the highest content of terpenoids from roots of crown explant attained 19.024%. The result of SDS PAGE showed proteins which were ±61 kD and ± 68 kD, each might be related to putative monoterpene synthase and sesquiterpenes complex, respectively.
[1] Vietmeyer, N. dan Ruskin, F. R. (1993): Vetiveria zizanoides (L.) Nash.
Indian Perfurmer, Current Sciences, 19, 35-73.
[2] Manalu, M. M., (2007): Produlsi Senyawa Metabolit Sekunder Melalui
Kultur Jaringan dan Transformasi Genetic Nicotiana Tabacum dan
Artemisia Annual. Tesis, Institut Teknologi Bandung.
[3] Vaniseree, M., Lee, C. Y., Lo, Nalawadee, S. M., Lin, C. Y. dan Tsay,
H. S., (2004): Studies on The Production of Some Important Secondary
Metabolites from Medicinal Plants Tissue Culture. Bot. Bull. Acad. Sin,
45, 1-22
[4] Leupin, R. E., (2001) : Vetiveria zizanioides: An Approach to Obtain
Essential Oil Variants via Tissue Culture, Disertasi, Swiss Federal
Institute of Technology Zürich
[5] Wulansari. A., (2010): Sintesis Induksi Minyak Vetiver Secara in vitro
Pada Kalus dan Kalus Berakar Vetiveria zizanoides, Tesis, Institut
Teknologi Bandung
[6] Dornenberg, H. dan Knorr, D., (1997): Challenges and Opportunities for
metabolite Production From Plant Cell And Tissue Cultures, Food
Technol, 51, 47- 54
[7] Toma, I., Zbughin, G., (2005): Histoanatomical Aspects of Aerial
Vegetative Organs of Stevia Rebaudiana Bertoni Cultivated In vitro,
Biologie Vegetală, 2, 29-38
[8] Mala, J., Gaudinova, A., Dobrev, P., Eder, J. dan Cvikora, M., (2006):
Role of Phythormones in Organogenic Ability of Elm Multiplicated
Shoots. Biol Plant, 50, 8-14
[9] Verpoorte, R., Heijden, V. R., Hoopen, H. J. G. dan Memelink, J.,
(1999). Metabolic Engineering of Plant Secondary Metabolite Pathways
for The Production of Fine Chemicals, Biotechnology Letters, 21, 467-
479
[10] Endress, R., (1994): Plant Cell Biotechnlogy, Springer-Verlag, Berlin,
53-58
[11] Nurcholis, W., (2008): Profil Senyawa Penciri dan Bioaktivitas
Tanaman Temulawak pada Agrobiofisik Berbeda, Tesis, Institut
Pertanian Bogor,
[12] Giurdice, L. D., Carata, E., Maurizo, Aifano, P., dan Vigliota, G.,
(2008): The Microbial Community of Vetiver root and its Involvement
into Essential Oil Biogenesis, Enviromental Microbiology, 10 (10),
2824-2841
[13] Wong, C. C., (2003): The Role of Mychorrizal Associated with Vetiveria
zizaniodes and Cyperus Polystachyos in The Remediation of Metals
(Lead and Zinc) Contaminated Soils, Thesis, Hong-Kong Baptist
University
[14] Fischbach, R.J., Zimmer, I., Steinbrecher, R., Pfichner, A. dan
Schnitzler, J.P., (2000) : Monoterpene Synthase Activities in Leaves of
Picea Abies (L.) Karst. dan Quercus ilex L, Phytochemistry, 54, 257-265
[15] Sen, S. E. dan Sperry, A. E., (2002): Partial Purification of a Farnesyl
Diphosphate Synthase from Whole-Body Manduca sexta. Insect,
Biochem Mol Biol, 32, 889-899
[16] Takahashi, I. dan Ogura, K., (1981): Farnesyl Pyrophosphate Synthase
from Bacillus subtilis, J Biochem, 89, 1581-1587
[17] Yan, L., Chun, Y. H. dan Feng, L. G., (2002): Cloning, E. coli
Expression and Molecular Analysis of a Novel Sesquiterpen Synthase
Gene From Artemisia Annua., Acta Botanica Sinica, 44, 1450-1455
[18] Schnee, C., Kollner, T. G., Gershenzon, J. dan Degenhardt, J., (2002):
The Maize Gene Terpene Synthase 1 Encodes a Sesquiterpene Synthase
Catalyzing The Formation of (E)-beta-farnesene, (E)-nerolidol, and
(E,E)-farnesol after Herbivore Damage, Plant Physiol, 130, 2049-2060
[19] Fischbach, R. J., Zimmer, I., Steinbrecher, R., Pfichner, A. dan
Schnitzler, J. P., (2000): Monoterpene Synthase Activities in Leaves of
Picea Abies (L.) Karst. dan Quercus ilex L, Phytochemistry, 54, 257-265
[20] Tholl, D., Chen, F., Petri, J., Gershenzon, J.dan Pichersky, E., (2005):
Two Sesquiterpene Synthases are Responsible for The Complex Mixture
of Sesquiterpenes Emitted from Arabidopsis Flowers, Plant J, 42, 757-
71
[21] McNaught, A., D. dan Wilkinson, A., (1997): Compendium of Chemical
Terminology, Blackwell Scientific Pub, Oxford, 1351.
[1] Vietmeyer, N. dan Ruskin, F. R. (1993): Vetiveria zizanoides (L.) Nash.
Indian Perfurmer, Current Sciences, 19, 35-73.
[2] Manalu, M. M., (2007): Produlsi Senyawa Metabolit Sekunder Melalui
Kultur Jaringan dan Transformasi Genetic Nicotiana Tabacum dan
Artemisia Annual. Tesis, Institut Teknologi Bandung.
[3] Vaniseree, M., Lee, C. Y., Lo, Nalawadee, S. M., Lin, C. Y. dan Tsay,
H. S., (2004): Studies on The Production of Some Important Secondary
Metabolites from Medicinal Plants Tissue Culture. Bot. Bull. Acad. Sin,
45, 1-22
[4] Leupin, R. E., (2001) : Vetiveria zizanioides: An Approach to Obtain
Essential Oil Variants via Tissue Culture, Disertasi, Swiss Federal
Institute of Technology Zürich
[5] Wulansari. A., (2010): Sintesis Induksi Minyak Vetiver Secara in vitro
Pada Kalus dan Kalus Berakar Vetiveria zizanoides, Tesis, Institut
Teknologi Bandung
[6] Dornenberg, H. dan Knorr, D., (1997): Challenges and Opportunities for
metabolite Production From Plant Cell And Tissue Cultures, Food
Technol, 51, 47- 54
[7] Toma, I., Zbughin, G., (2005): Histoanatomical Aspects of Aerial
Vegetative Organs of Stevia Rebaudiana Bertoni Cultivated In vitro,
Biologie Vegetală, 2, 29-38
[8] Mala, J., Gaudinova, A., Dobrev, P., Eder, J. dan Cvikora, M., (2006):
Role of Phythormones in Organogenic Ability of Elm Multiplicated
Shoots. Biol Plant, 50, 8-14
[9] Verpoorte, R., Heijden, V. R., Hoopen, H. J. G. dan Memelink, J.,
(1999). Metabolic Engineering of Plant Secondary Metabolite Pathways
for The Production of Fine Chemicals, Biotechnology Letters, 21, 467-
479
[10] Endress, R., (1994): Plant Cell Biotechnlogy, Springer-Verlag, Berlin,
53-58
[11] Nurcholis, W., (2008): Profil Senyawa Penciri dan Bioaktivitas
Tanaman Temulawak pada Agrobiofisik Berbeda, Tesis, Institut
Pertanian Bogor,
[12] Giurdice, L. D., Carata, E., Maurizo, Aifano, P., dan Vigliota, G.,
(2008): The Microbial Community of Vetiver root and its Involvement
into Essential Oil Biogenesis, Enviromental Microbiology, 10 (10),
2824-2841
[13] Wong, C. C., (2003): The Role of Mychorrizal Associated with Vetiveria
zizaniodes and Cyperus Polystachyos in The Remediation of Metals
(Lead and Zinc) Contaminated Soils, Thesis, Hong-Kong Baptist
University
[14] Fischbach, R.J., Zimmer, I., Steinbrecher, R., Pfichner, A. dan
Schnitzler, J.P., (2000) : Monoterpene Synthase Activities in Leaves of
Picea Abies (L.) Karst. dan Quercus ilex L, Phytochemistry, 54, 257-265
[15] Sen, S. E. dan Sperry, A. E., (2002): Partial Purification of a Farnesyl
Diphosphate Synthase from Whole-Body Manduca sexta. Insect,
Biochem Mol Biol, 32, 889-899
[16] Takahashi, I. dan Ogura, K., (1981): Farnesyl Pyrophosphate Synthase
from Bacillus subtilis, J Biochem, 89, 1581-1587
[17] Yan, L., Chun, Y. H. dan Feng, L. G., (2002): Cloning, E. coli
Expression and Molecular Analysis of a Novel Sesquiterpen Synthase
Gene From Artemisia Annua., Acta Botanica Sinica, 44, 1450-1455
[18] Schnee, C., Kollner, T. G., Gershenzon, J. dan Degenhardt, J., (2002):
The Maize Gene Terpene Synthase 1 Encodes a Sesquiterpene Synthase
Catalyzing The Formation of (E)-beta-farnesene, (E)-nerolidol, and
(E,E)-farnesol after Herbivore Damage, Plant Physiol, 130, 2049-2060
[19] Fischbach, R. J., Zimmer, I., Steinbrecher, R., Pfichner, A. dan
Schnitzler, J. P., (2000): Monoterpene Synthase Activities in Leaves of
Picea Abies (L.) Karst. dan Quercus ilex L, Phytochemistry, 54, 257-265
[20] Tholl, D., Chen, F., Petri, J., Gershenzon, J.dan Pichersky, E., (2005):
Two Sesquiterpene Synthases are Responsible for The Complex Mixture
of Sesquiterpenes Emitted from Arabidopsis Flowers, Plant J, 42, 757-
71
[21] McNaught, A., D. dan Wilkinson, A., (1997): Compendium of Chemical
Terminology, Blackwell Scientific Pub, Oxford, 1351.
@article{"International Journal of Biological, Life and Agricultural Sciences:65044", author = "Rizkita R. Esyanti and Iriawati and Olga Mardisadora", title = "Vetiver Oil Production from Root Culture of Vetiveria zizanioides", abstract = "Vetiver oil is secondary metabolite that accumulates in Vetiveria zizanioides roots. The aim of this study was to obtain best type of root culture which produce high amount of vetiver oil, and was similar to metabolites produce from its mother plant. Protein analysis was also conducted to detect protein, related to putative enzymes, which have a role in terpenoids synthesis in the root culture. The results showed that root culture derived from crown explant produced the best root growth. The root culture produced primary and lateral roots, ca. 40 branches. The vetiver oil produced from root culture was analyzed by using GC-MS., and the highest content of terpenoids from roots of crown explant attained 19.024%. The result of SDS PAGE showed proteins which were ±61 kD and ± 68 kD, each might be related to putative monoterpene synthase and sesquiterpenes complex, respectively.
", keywords = "Protein, Root culture, Terpenoids, Vetiver oil.", volume = "7", number = "9", pages = "864-4", }
