Using Malolactic Fermentation with Acid- And Ethanol- Adapted Oenococcus Oeni Strain to Improve the Quality of Wine from Champs Bourcin Grape in Sapa - Lao Cai

Champs Bourcin black grape originated from Aquitaine, France and planted in Sapa, Lao cai provice, exhibited high total acidity (11.72 g/L). After 9 days of alcoholic fermentation at 25oC using Saccharomyces cerevisiae UP3OY5 strain, the ethanol concentration of wine was 11.5% v/v, however the sharp sour taste of wine has been found. The malolactic fermentation (MLF) was carried out by Oenococcus oeni ATCCBAA-1163 strain which had been preadapted to acid (pH 3-4) and ethanol (8-12%v/v) conditions. We obtained the highest vivability (83.2%) upon malolactic fermentation after 5 days at 22oC with early stationary phase O. oeni cells preadapted to pH 3.5 and 8% v/v ethanol in MRS medium. The malic acid content in wine was decreased from 5.82 g/L to 0.02 g/L after MLF (21 days at 22oC). The sensory quality of wine was significantly improved.

Authors:

• Pham Thu Thuy
• Nguyen Lan Huong
• Chu Ky Son

Keywords:

• Champs Bourcin grape
• malolactic fermentation
• pre-adaptation
• Oenococcus oeni

References:

[1] P. G. P. Versari. A, and Cattaneo. M., "Leuconostoc oenos and
malolactic fermentation in wine: a review," J. Ind. Microbiol., vol. 23,
pp. 447- 455, 1999.
[2] A. Lonvaud-Funel, "Lactic acid bacteria in the quality improvement and
depreciation of wine," Antonie Van Leeuwenhoek, vol. 76, pp. 317-331,
1999.
[3] S. Q. Liu, " A review: malolactic fermentation in wine beyond
deacidification," J Appl Microbiol., vol. 92, pp. 589-601, 2002.
[4] H. Teixeira, M. G. Goncalves, N. Rozes, A. Ramos, and M. V. San
Romao, "Lactobacillic acid accumulation in the plasma membrane of
Oenococcus oeni: a response to ethanol stress," Microb. Ecol., vol. 43,
pp. 146-153, 2002.
[5] R. L. Lopez I., P. Santamaria, C. Torres, and F. Ruiz-Larrea,
"Performance of malolactic fermentation by inoculation of selected
Lactobacillus plantarum and Oenococcus oeni strains isolated from
Rioja red wines," Vitis, vol. 47, pp. 123-129, 2008.
[6] C. T. Lopez I, M. Zarazaga, C. Torres, F. Ruiz-Larrea, "Evidence of
mixed wild populations Oenococcus oeni strains of during wine
spontaneous malolactic fermentations," Eur. Food Res. Technol., vol.
226, pp. 215-232, 2007.
[7] J. Guzzo, M. P. Jobin, F. Delmas, L. C. Fortier, D. Garmyn, R. Tourdot-
Marechal, B. Lee, and C. Divies, "Regulation of stress response in
Oenococcus oeni as a function of environmental changes and growth
phase," Int. J. Food Microbiol. , vol. 55, pp. 27-31, 2000.
[8] A. Bastianini, L. Granchi, S. Guerrini, and M. Vincenzini. , "Fatty acid
composition of malolactic Oenococcus oeni strains exposed to pH and
ethanol stress," Ital. J. Food Sci. , vol. 12, pp. 333-342, 2000.
[9] P. G.-C. Maicas S., S. Ferrer, and I. Pardo, "Production of Oenococcus
oeni biomass to induce malolactic fermentation in wine by control of pH
and substrate addition," Biotechnology Letters, vol. 21, pp. 349-353,
1999.