The Effect of Curcumin on Cryopreserved Bovine Semen
Oxidative stress associated with semen cryopreservation may result in lipid peroxidation (LPO), DNA damage and apoptosis, leading to decreased sperm motility and fertilization ability. Curcumin (CUR), a natural phenol isolated from Curcuma longa Linn. has been presented as a possible supplement for a more effective semen cryopreservation because of its antioxidant properties. This study focused to evaluate the effects of CUR on selected oxidative stress parameters in cryopreserved bovine semen. 20 bovine ejaculates were split into two aliquots and diluted with a commercial semen extender containing CUR (50 μmol/L) or no supplement (control), cooled to 4 °C, frozen and kept in liquid nitrogen. Frozen straws were thawed in a water bath for subsequent experiments. Computer assisted semen analysis was used to evaluate spermatozoa motility, and reactive oxygen species (ROS) generation was quantified by using luminometry. Superoxide generation was evaluated with the NBT test, and LPO was assessed via the TBARS assay. CUR supplementation significantly (P<0.001) increased the spermatozoa motility and provided a significantly higher protection against ROS (P<0.001) or superoxide (P<0.01) overgeneration caused by semen freezing and thawing. Furthermore, CUR administration resulted in a significantly (P<0.01) lower LPO of the experimental semen samples. In conclusion, CUR exhibits significant ROS-scavenging activities which may prevent oxidative insults to cryopreserved spermatozoa and thus may enhance the post-thaw functional activity of male gametes.
[1] J. L. Bailey, J. F. Bilodeau, and N. Cormier, “Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon,” Journal of Andrology, vol. 21, p. 1-7, 2000.
[2] J. Bailey, A. Morrier, and N. Cormier, “Semen cryopreservation: Successes and persistent problems in farm species,” Canadian Journal of Animal Science, vol. 83, pp. 393-401, 2003.
[3] W. T. Holt, “Basic aspects of frozen storage of semen,” Animal Reproduction Science, vol. 62, pp. 3-22, 2000.
[4] W. T. Holt, “Fundamental aspects of sperm cryobiology: the importance of species and individual differences,” Theriogenology, vol. 53, pp. 47-58, 2000.
[5] J. G. Alvarez, and B. T. Storey, “Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility,” Biology of Reproduction, vol. 29, pp. 548-555, 1983.
[6] H. P. Nissen, and H.W. Kreysel, “Superoxide dismutase in human semen,” Klinische Wochenschrift, vol. 61, pp. 63-65, 1983.
[7] S. Chatterjee, E. De Lamirande, and C. Gagnon, “Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione,” Molecular Reproduction and Development, vol. 60, pp. 498-506, 2001.
[8] J. Gadea, E. Selles, M. A. Marco, P. Coy, C. Matas, R. Romar, and S. Ruiz, “Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders,” Theriogenology, vol. 62, pp. 690-701, 2004.
[9] A. K. Bansal, and G. S. Bilaspuri, “Impacts of oxidative stress and antioxidants on semen functions,” Veterinary Medicine International, 2011, doi:10.4061/2011/686137.
[10] A. C. Reddy, and B. R. Lokesh, “Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron,” Molecular and Cellular Biochemistry,” vol. 137, pp. 1-8, 1994.
[11] M. K. Unnikrishnan, and M. N. Rao, “Curcumin inhibits nitrogen dioxide induced oxidation of hemoglobin,” Molecular and Cellular Biochemistry,” vol. 146, pp. 35-37, 1995.
[12] Sreejayan, and M. N. Rao, “Curcuminoids as potent inhibitors of lipid peroxidation,” Journal of Pharmacy and Pharmacology, vol. 46, pp. 1013-1016, 1994.
[13] M. R. Salahshoor, C. Jalili, M. Khazaei, and F. Khani, “Effects of curcumin on reproductive parameters in male mice,” Journal of Clinical Research in Paramedical Sciences, vol. 1, pp. 1-3, 2012.
[14] M. N. Bucak, N. Basspinar, B. P. Tuncer, K. Coyan, S. Sariozkan, P. P. Akalin, S. Buyukleblebici, and S. Kucukgunay, “Effects of curcumin and dithioerythritol on frozen-thawed bovine semen,” Andrologia, vol. 44, pp. 102-109, 2012.
[15] A. Soleimanzadeh, and A. Saberivand, “Effect of curcumin on rat sperm morphology after the freeze-thawing process,” Veterinary Research Forum, vol. 4, pp. 185-189, 2013.
[16] R. J. Naz, “Can curcumin provide an ideal contraceptive?” Molecular Reproduction and Development, vol. 78, pp. 116-123, 2011.
[17] E. Tvrdá, N. Lukáč, T. Jambor, J. Lukáčová, and P. Massányi, P., “Curcumin in male fertility: effects on spermatozoa vitality and oxidative balance,” Journal of Microbiology, Biotechnology and Food Sciences, vol. 4, pp. 120-124, 2015.
[18] E. Tvrdá, E. Tušimová, A. Kováčik, D. Paál, H. Greifová, A. Abdramanov, and N. Lukáč, “Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress,” Animal Reproduction Science, 2016, in press.
[19] A. H. Kashou, R. Sharma, and A. Agarwal, “Assessment of oxidative stress in sperm and semen,” Methods in Molecular Biology, vol. 927, 351-361, 2013.
[20] B. A. Ball, “Oxidative stress, osmotic stress and apoptosis: impacts on sperm function and preservation in the horse,” Animal Reproduction Science, vol. 107, pp. 257-267, 2008.
[21] C. Tatone, G. Di Emidio, M. Vento, R. Ciriminna, and P. G. Artini, “Cryopreservation and oxidative stress in reproductive cells. Gynecological Endocrinology, vol. 26, p. 563-567, 2010.
[22] M. N. Bucak, A. Atessahin, and A. Yuce, “Effect of antioxidants and oxidative stress parameters on ram semen after the freeze-thawing process,” Small Ruminant Research, vol. 75, pp. 128-133, 2008.
[23] M. N. Bucak, S. Sariozkan, P. B. Tuncer, F. Sakin, A. Atessahin, R. Kulaksız, and M. Cevik, “The effect of antioxidants on post-thawed Angoragoat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities,” Small Ruminant Research, vol. 89, pp. 24-30, 2010.
[24] J. Elia, N. Imbrogno, M. Delfino, R. Mazzilli, T. Rossi, and F. Mazzilli, “The importance of the sperm motility classes-future directions. Open Andrology Journal, vol. 2, pp. 42-43, 2010.
[25] A. Agarwal, R. A. Saleh, and M. A. Bedaiwy, “Role of reactive oxygen species in the pathophysiology of human reproduction,” Fertility and Sterility, vol.79, pp. 829-843, 2003.
[26] K. Rahman, “Studies on free radicals, antioxidants, and co-factors,” Journal of Clinical Interventions in Aging, vol. 2, pp. 219-236, 2007.
[27] M. Ishihara, M. Itoh, K. Miyamoto, S. Suna, Y. Takeuchi, I. Takenaka, and F. Jitsunari, F., “Spermatogenic disturbance induced bydi-(2-ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat,” International Journal of Andrology, vol. 23, pp. 85-94, 2000.
[28] A. F. Salama, and S. M. El-Bahr, “Effect of curcumin on cadmium induced oxidative testicular damage in rats,” Journal of Medical Research Institute, vol. 28, pp. 167-173, 2007.
[1] J. L. Bailey, J. F. Bilodeau, and N. Cormier, “Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon,” Journal of Andrology, vol. 21, p. 1-7, 2000.
[2] J. Bailey, A. Morrier, and N. Cormier, “Semen cryopreservation: Successes and persistent problems in farm species,” Canadian Journal of Animal Science, vol. 83, pp. 393-401, 2003.
[3] W. T. Holt, “Basic aspects of frozen storage of semen,” Animal Reproduction Science, vol. 62, pp. 3-22, 2000.
[4] W. T. Holt, “Fundamental aspects of sperm cryobiology: the importance of species and individual differences,” Theriogenology, vol. 53, pp. 47-58, 2000.
[5] J. G. Alvarez, and B. T. Storey, “Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility,” Biology of Reproduction, vol. 29, pp. 548-555, 1983.
[6] H. P. Nissen, and H.W. Kreysel, “Superoxide dismutase in human semen,” Klinische Wochenschrift, vol. 61, pp. 63-65, 1983.
[7] S. Chatterjee, E. De Lamirande, and C. Gagnon, “Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione,” Molecular Reproduction and Development, vol. 60, pp. 498-506, 2001.
[8] J. Gadea, E. Selles, M. A. Marco, P. Coy, C. Matas, R. Romar, and S. Ruiz, “Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders,” Theriogenology, vol. 62, pp. 690-701, 2004.
[9] A. K. Bansal, and G. S. Bilaspuri, “Impacts of oxidative stress and antioxidants on semen functions,” Veterinary Medicine International, 2011, doi:10.4061/2011/686137.
[10] A. C. Reddy, and B. R. Lokesh, “Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron,” Molecular and Cellular Biochemistry,” vol. 137, pp. 1-8, 1994.
[11] M. K. Unnikrishnan, and M. N. Rao, “Curcumin inhibits nitrogen dioxide induced oxidation of hemoglobin,” Molecular and Cellular Biochemistry,” vol. 146, pp. 35-37, 1995.
[12] Sreejayan, and M. N. Rao, “Curcuminoids as potent inhibitors of lipid peroxidation,” Journal of Pharmacy and Pharmacology, vol. 46, pp. 1013-1016, 1994.
[13] M. R. Salahshoor, C. Jalili, M. Khazaei, and F. Khani, “Effects of curcumin on reproductive parameters in male mice,” Journal of Clinical Research in Paramedical Sciences, vol. 1, pp. 1-3, 2012.
[14] M. N. Bucak, N. Basspinar, B. P. Tuncer, K. Coyan, S. Sariozkan, P. P. Akalin, S. Buyukleblebici, and S. Kucukgunay, “Effects of curcumin and dithioerythritol on frozen-thawed bovine semen,” Andrologia, vol. 44, pp. 102-109, 2012.
[15] A. Soleimanzadeh, and A. Saberivand, “Effect of curcumin on rat sperm morphology after the freeze-thawing process,” Veterinary Research Forum, vol. 4, pp. 185-189, 2013.
[16] R. J. Naz, “Can curcumin provide an ideal contraceptive?” Molecular Reproduction and Development, vol. 78, pp. 116-123, 2011.
[17] E. Tvrdá, N. Lukáč, T. Jambor, J. Lukáčová, and P. Massányi, P., “Curcumin in male fertility: effects on spermatozoa vitality and oxidative balance,” Journal of Microbiology, Biotechnology and Food Sciences, vol. 4, pp. 120-124, 2015.
[18] E. Tvrdá, E. Tušimová, A. Kováčik, D. Paál, H. Greifová, A. Abdramanov, and N. Lukáč, “Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress,” Animal Reproduction Science, 2016, in press.
[19] A. H. Kashou, R. Sharma, and A. Agarwal, “Assessment of oxidative stress in sperm and semen,” Methods in Molecular Biology, vol. 927, 351-361, 2013.
[20] B. A. Ball, “Oxidative stress, osmotic stress and apoptosis: impacts on sperm function and preservation in the horse,” Animal Reproduction Science, vol. 107, pp. 257-267, 2008.
[21] C. Tatone, G. Di Emidio, M. Vento, R. Ciriminna, and P. G. Artini, “Cryopreservation and oxidative stress in reproductive cells. Gynecological Endocrinology, vol. 26, p. 563-567, 2010.
[22] M. N. Bucak, A. Atessahin, and A. Yuce, “Effect of antioxidants and oxidative stress parameters on ram semen after the freeze-thawing process,” Small Ruminant Research, vol. 75, pp. 128-133, 2008.
[23] M. N. Bucak, S. Sariozkan, P. B. Tuncer, F. Sakin, A. Atessahin, R. Kulaksız, and M. Cevik, “The effect of antioxidants on post-thawed Angoragoat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities,” Small Ruminant Research, vol. 89, pp. 24-30, 2010.
[24] J. Elia, N. Imbrogno, M. Delfino, R. Mazzilli, T. Rossi, and F. Mazzilli, “The importance of the sperm motility classes-future directions. Open Andrology Journal, vol. 2, pp. 42-43, 2010.
[25] A. Agarwal, R. A. Saleh, and M. A. Bedaiwy, “Role of reactive oxygen species in the pathophysiology of human reproduction,” Fertility and Sterility, vol.79, pp. 829-843, 2003.
[26] K. Rahman, “Studies on free radicals, antioxidants, and co-factors,” Journal of Clinical Interventions in Aging, vol. 2, pp. 219-236, 2007.
[27] M. Ishihara, M. Itoh, K. Miyamoto, S. Suna, Y. Takeuchi, I. Takenaka, and F. Jitsunari, F., “Spermatogenic disturbance induced bydi-(2-ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat,” International Journal of Andrology, vol. 23, pp. 85-94, 2000.
[28] A. F. Salama, and S. M. El-Bahr, “Effect of curcumin on cadmium induced oxidative testicular damage in rats,” Journal of Medical Research Institute, vol. 28, pp. 167-173, 2007.
@article{"International Journal of Biological, Life and Agricultural Sciences:74204", author = "Eva Tvrdá and Marek Halenár and Hana Greifová and Alica Mackovich and Faridullah Hashim and Norbert Lukáč", title = "The Effect of Curcumin on Cryopreserved Bovine Semen", abstract = "Oxidative stress associated with semen cryopreservation may result in lipid peroxidation (LPO), DNA damage and apoptosis, leading to decreased sperm motility and fertilization ability. Curcumin (CUR), a natural phenol isolated from Curcuma longa Linn. has been presented as a possible supplement for a more effective semen cryopreservation because of its antioxidant properties. This study focused to evaluate the effects of CUR on selected oxidative stress parameters in cryopreserved bovine semen. 20 bovine ejaculates were split into two aliquots and diluted with a commercial semen extender containing CUR (50 μmol/L) or no supplement (control), cooled to 4 °C, frozen and kept in liquid nitrogen. Frozen straws were thawed in a water bath for subsequent experiments. Computer assisted semen analysis was used to evaluate spermatozoa motility, and reactive oxygen species (ROS) generation was quantified by using luminometry. Superoxide generation was evaluated with the NBT test, and LPO was assessed via the TBARS assay. CUR supplementation significantly (P", keywords = "Bulls, cryopreservation, curcumin, lipid peroxidation, reactive oxygen species, spermatozoa.", volume = "10", number = "11", pages = "707-5", }
