Sophorolipids Production by Candida Bombicola using Synthetic Dairy Wastewater
Sophorolipids (SLs) production by the yeast Candida
bombicola was studied in batch shake flasks using synthetic dairy
wastewaters (SDWW) with or without any added external carbon and
nitrogen sources. A maximum SLs production of 38.76 g/l was
observed with the SDWW supplemented with low cost substrate of
sugarcane molasses at 50 g/l and soybean oil at 50 g/l. When the
SDWW was supplemented with more costly glucose, yeast extract,
urea and soybean oil, the production, however, got lowered to only
29.49 g/l, but with a maximum biomass production of 17.38 g/l
together with a complete utilization of the carbon sources.
[1] R. K. Hommel, and K. Huse, "Regulation of sophorose lipid production
by Candida (Torulopsis) apicola," Biotechnol. Lett., 1993, vol 15, pp.
853-858.
[2] 2. H. J. Asmer, S. Lang, F. Wagner, and V. Wray, "Microbial
production, structure elucidation and bioconversion of sophorose lipids,"
JAOCS, 1988, vol. 65, pp. 1460-1466.
[3] A. P. J. Gorin, J. F. T. Spencer, and A. P. Tulloch, "Hydroxy fatty acid
glycosides of sophorose from Torulopsis magnoliae," Can. J. Chem.,
1961, vol. 39, pp. 846-895.
[4] 4. D. K. Y. Solaiman, R. D. Ashby, J. A. Zerkowski, and T. A. Foglia,
"Simplified soy molasses-based medium for reduced-cost production of
sophorolipids by Candida bombicola," Biotechnol. Lett., 2007, vol. 29,
pp. 1341-1347.
[5] U. Rau, S. Hammen, R. Heckmann, V. Wray, and S. Lang,
"Sophorolipids: a source for novel compounds," Ind. Crop Prod. 2001,
vol. 13, pp. 85-92.
[6] C. N. Mulligan, R. N. Yong, and B. F. Gibbs, "Heavy metal removal
from sediments by biosurfactants," J. Hazard. Mater. 2001, vol 85, pp.
111-125.
[7] 7. C. Schippers, K. Gessner, T. Muller, and T. Scheper, "Microbial
degradation of phenanthrene by addition of a sophorolipid mixture," J.
Biotechnol. 2000, vol 83, pp. 189-198.
[8] L. R. Lynd, C. E. Wyman, and T. U. Gerngross, "Biocommodity
engineering," Biotechnol. Prog. 1999, vol 15, pp. 777-793.
[9] A. Daverey, and K. Pakshirajan, Production, characterization, and
properties of sophorolipids from the yeast Candida bombicola using a
low-cost fermentative medium. Appl. Biochem. Biotechnol., to be
published.
[10] M. Deshpande, L. Daniels, "Evaluation of sophorolipid biosurfactant
production by Candida bombicola using animal fat," Bioresour.
Technol. 1995, vol. 54, pp. 143-150.
[11] B. D. Tripathi, and A. R. Upadhyay, "Dairy effluent polishing by aquatic
macrophytes," Water Air Soil Pollut., 2003, vol. 143, pp. 377- 385.
[12] R. Willey, "Fats, oils and greases: The minimization and treatment of
wastewaters generated from oil refining and margarine production,"
Ecotoxicol. Environ. Saf., 2001, vol. 50, pp. 127-133.
[13] M. C. Cammarota, and D. M. G. Freire, "A review on hydrolytic
enzymes in the treatment of wastewater with high oil and grease
content," Bioresour. Technol., 2006, vol. 97, pp. 2195-2210.
[14] K. Tano-Debrah, S. Fukuyama, N. Otonari, F. Taniguchi, and M. Ogura,
"An inoculum for the aerobic treatment of wastewaters with high
concentrations of fats and oils," Bioresour. Technol., 1999, vol. 69, pp.
133-139.
[15] L. Masse, K. J. Kennedy, and S. Chou, "Testing of alkaline and
enzymatic hydrolysis pretreatments for oil particles in slaughterhouse
wastewater" Bioresour. Technol., 2001, vol. 77, pp. 145-155.
[16] G. Sivalingam, S. Chattopadhyay, and G. Madras, "Solvent effects on
lipase catalyzed biodegradation of poly (epsilon-caprolactone) in
solution," Polym. Degrad. Stab., 2003, vol. 79, pp. 413-418.
[17] M. C. M. R. Leal, D. M. G. Freire, M. C. Cammarota, and G. L.
Sant-Anna Jr., "Effect of enzymatic hydrolysis on anaerobic treatment of
dairy wastewater," Process Biochem. 2006, vol. 41, pp. 1173-1178.
[18] S. Joshi, C. Bharucha, S. Jha, S. Yadav, A. Nerurkar, and A. J. Desai,
"Biosurfactant production using molasses and whey under thermophilic
conditions," Bioresour. Technol., 2008, vol. 99, pp. 195-199.
[19] T. A. Scott, Jr., and E. H. Melvin, "Determination of dextran with
anthrone," Anal. Chem., 1953, vol. 25, pp. 1656-1661.
[20] Y. H. Hu, and L-K. Ju, "Sophorolipid production from different lipid
precursors observed with LC-MS," Enzyme Microb. Technol., 2001, vol.
29, pp. 593-601.
[21] 21. A. Daverey, and K. Pakshirajan, Production of sophorolipids by the
yeast Candida bombicola using simple and low cost fermentative
media," Food Res. Intern., to be published.
[1] R. K. Hommel, and K. Huse, "Regulation of sophorose lipid production
by Candida (Torulopsis) apicola," Biotechnol. Lett., 1993, vol 15, pp.
853-858.
[2] 2. H. J. Asmer, S. Lang, F. Wagner, and V. Wray, "Microbial
production, structure elucidation and bioconversion of sophorose lipids,"
JAOCS, 1988, vol. 65, pp. 1460-1466.
[3] A. P. J. Gorin, J. F. T. Spencer, and A. P. Tulloch, "Hydroxy fatty acid
glycosides of sophorose from Torulopsis magnoliae," Can. J. Chem.,
1961, vol. 39, pp. 846-895.
[4] 4. D. K. Y. Solaiman, R. D. Ashby, J. A. Zerkowski, and T. A. Foglia,
"Simplified soy molasses-based medium for reduced-cost production of
sophorolipids by Candida bombicola," Biotechnol. Lett., 2007, vol. 29,
pp. 1341-1347.
[5] U. Rau, S. Hammen, R. Heckmann, V. Wray, and S. Lang,
"Sophorolipids: a source for novel compounds," Ind. Crop Prod. 2001,
vol. 13, pp. 85-92.
[6] C. N. Mulligan, R. N. Yong, and B. F. Gibbs, "Heavy metal removal
from sediments by biosurfactants," J. Hazard. Mater. 2001, vol 85, pp.
111-125.
[7] 7. C. Schippers, K. Gessner, T. Muller, and T. Scheper, "Microbial
degradation of phenanthrene by addition of a sophorolipid mixture," J.
Biotechnol. 2000, vol 83, pp. 189-198.
[8] L. R. Lynd, C. E. Wyman, and T. U. Gerngross, "Biocommodity
engineering," Biotechnol. Prog. 1999, vol 15, pp. 777-793.
[9] A. Daverey, and K. Pakshirajan, Production, characterization, and
properties of sophorolipids from the yeast Candida bombicola using a
low-cost fermentative medium. Appl. Biochem. Biotechnol., to be
published.
[10] M. Deshpande, L. Daniels, "Evaluation of sophorolipid biosurfactant
production by Candida bombicola using animal fat," Bioresour.
Technol. 1995, vol. 54, pp. 143-150.
[11] B. D. Tripathi, and A. R. Upadhyay, "Dairy effluent polishing by aquatic
macrophytes," Water Air Soil Pollut., 2003, vol. 143, pp. 377- 385.
[12] R. Willey, "Fats, oils and greases: The minimization and treatment of
wastewaters generated from oil refining and margarine production,"
Ecotoxicol. Environ. Saf., 2001, vol. 50, pp. 127-133.
[13] M. C. Cammarota, and D. M. G. Freire, "A review on hydrolytic
enzymes in the treatment of wastewater with high oil and grease
content," Bioresour. Technol., 2006, vol. 97, pp. 2195-2210.
[14] K. Tano-Debrah, S. Fukuyama, N. Otonari, F. Taniguchi, and M. Ogura,
"An inoculum for the aerobic treatment of wastewaters with high
concentrations of fats and oils," Bioresour. Technol., 1999, vol. 69, pp.
133-139.
[15] L. Masse, K. J. Kennedy, and S. Chou, "Testing of alkaline and
enzymatic hydrolysis pretreatments for oil particles in slaughterhouse
wastewater" Bioresour. Technol., 2001, vol. 77, pp. 145-155.
[16] G. Sivalingam, S. Chattopadhyay, and G. Madras, "Solvent effects on
lipase catalyzed biodegradation of poly (epsilon-caprolactone) in
solution," Polym. Degrad. Stab., 2003, vol. 79, pp. 413-418.
[17] M. C. M. R. Leal, D. M. G. Freire, M. C. Cammarota, and G. L.
Sant-Anna Jr., "Effect of enzymatic hydrolysis on anaerobic treatment of
dairy wastewater," Process Biochem. 2006, vol. 41, pp. 1173-1178.
[18] S. Joshi, C. Bharucha, S. Jha, S. Yadav, A. Nerurkar, and A. J. Desai,
"Biosurfactant production using molasses and whey under thermophilic
conditions," Bioresour. Technol., 2008, vol. 99, pp. 195-199.
[19] T. A. Scott, Jr., and E. H. Melvin, "Determination of dextran with
anthrone," Anal. Chem., 1953, vol. 25, pp. 1656-1661.
[20] Y. H. Hu, and L-K. Ju, "Sophorolipid production from different lipid
precursors observed with LC-MS," Enzyme Microb. Technol., 2001, vol.
29, pp. 593-601.
[21] 21. A. Daverey, and K. Pakshirajan, Production of sophorolipids by the
yeast Candida bombicola using simple and low cost fermentative
media," Food Res. Intern., to be published.
@article{"International Journal of Biological, Life and Agricultural Sciences:60682", author = "A. Daverey and K. Pakshirajan and P. Sangeetha", title = "Sophorolipids Production by Candida Bombicola using Synthetic Dairy Wastewater", abstract = "Sophorolipids (SLs) production by the yeast Candida
bombicola was studied in batch shake flasks using synthetic dairy
wastewaters (SDWW) with or without any added external carbon and
nitrogen sources. A maximum SLs production of 38.76 g/l was
observed with the SDWW supplemented with low cost substrate of
sugarcane molasses at 50 g/l and soybean oil at 50 g/l. When the
SDWW was supplemented with more costly glucose, yeast extract,
urea and soybean oil, the production, however, got lowered to only
29.49 g/l, but with a maximum biomass production of 17.38 g/l
together with a complete utilization of the carbon sources.", keywords = "Candida bombicola, dairy wastewater, fat and oil,
sophorolipids.", volume = "3", number = "3", pages = "154-3", }
