Separation of Polyphenolics and Sugar by Ultrafiltration: Effects of Operating Conditions on Fouling and Diafiltration

Polyphenolics and sugar are the components of many fruit juices. In this work, the performance of ultra-filtration (UF) for separating phenolic compounds from apple juice was studied by performing batch experiments in a membrane module with an area of 0.1 m2 and fitted with a regenerated cellulose membrane of 1 kDa MWCO. The effects of various operating conditions: transmembrane pressure (3, 4, 5 bar), temperature (30, 35, 40 ºC), pH (2, 3, 4, 5), feed concentration (3, 5, 7, 10, 15 ºBrix for apple juice) and feed flow rate (1, 1.5, 1.8 L/min) on the performance were determined. The optimum operating conditions were: transmembrane pressure 4 bar, temperature 30 ºC, feed flow rate 1 – 1.8 L/min, pH 3 and 10 Brix (apple juice). After performing ultrafiltration under these conditions, the concentration of polyphenolics in retentate was increased by a factor of up to 2.7 with up to 70% recovered in the permeate and with approx. 20% of the sugar in that stream.. Application of diafiltration (addition of water to the concentrate) can regain the flux by a factor of 1.5, which has been decreased due to fouling. The material balance performed on the process has shown the amount of deposits on the membrane and the extent of fouling in the system. In conclusion, ultrafiltration has been demonstrated as a potential technology to separate the polyphenolics and sugars from their mixtures and can be applied to remove sugars from fruit juice.

Authors:

• Diqiao S. Wei
• M. Hossain
• Zaid S. Saleh

Keywords:

• Fouling
• membrane
• polyphenols
• ultrafiltration.

References:

[1] Nawaz, H., Shi, J., Mittal, G. S., Kakuda, Y. (2006). "Extraction of
polyphenols from grape seeds and concentration by ultrafiltration." Sep.
Purif. Technol. 48(2), pp: 176-181.
[2] Alper, N. and Acar, J. (2004). "Removal of phenolic compounds in
pomegranate juice using ultrafiltration and laccase-ultrafiltration
combinations." Nahrung - Food 48(3), pp: 184-187.
[3] Kammerer, D. R., Schieber, A., Carle, R.et al. (2005). "Characterization
and recovery of phenolic compounds from grape pomace - a review." J.
Appl. Bot. Food Quality/Angewandte Botanik 79(3), pp: 189-196.
[4] Mondor, M., Girard, B., Moresoli, C. (2000). "Modeling flux behavior
for membrane filtration of apple juice." Food Res. Intl. 33(7), pp: 539-
548.
[5] De Bruijn, J. P. F., Venegas, A., Martínez, J.A., Bórquez, R.(2003).
"Ultrafiltration performance of Carbosep membranes for the clarification
of apple juice." Food Sci. Technol. 36(4), pp: 397-406.
[6] Alvarez, V., Andres, L.J., Riera, F., Alvarez,R. (1996). "Microfiltration
of apple juice using inorganic membranes: Process optimization and
juice stablity." Canadian J. Chem. Eng. 74, pp: 156-162.
[7] Saleh, Z. S., R. Stanley, R., Nigam, M. (2006). "Separation and
Concentration of Health Compounds by Membrane Filtration." Intl. J.
Food Eng. 2(3).
[8] Tanada-Palmu, P., Jardine, J., Matta, V. (1999). "Production of a banana
(Musa cavendishii) extract containing no polyphenol oxidase by
ultrafiltration." J. Sci. Food Agric. 79(5), pp: 643-647.
[9] Kallioinen, M., Pekkarinen, M., Manttari, M., Nystrom, M., Nuortila-
Jokinen, J. (2006). "Stability of two different regenerated cellulose
ultrafiltration membrane under varying solution temperature."
Desalination 199, pp: 204-206.
[10] Balakrishnan, M., Dua, M., Bhagat, J. J. (2000). "Effect of operating
parameters on sugarcane juice ultrafiltration: Results of a field
experience." Separation and Purification Technology 19(3), pp: 209-220.
[11] Karode, S. K., Gupta, B. B., Courtois, T. (2000). "Ultrafiltration of raw
Indian sugar solution using polymeric and mineral membranes." Sep.
Sci. Technol. 35(15), pp : 2473-2483.
[12] Vladisavljevic, G. T., Vukosavljevic, P., Bukvic, B. (2003). "Permeate
flux and fouling resistance in ultrafiltration of depectinizad apple juice
using ceramic membranes." J. of Food Eng. 60, pp: 241-247.