Quantitative Characteristics of Rainbow Trout, Oncorhynchus Mykiss, Neo-Males (XX Genotype) and Super-Males (YY Genotype) Sperm
Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in samples of stripped and testicular sperm obtained from XY and YY males, and testicular sperm of XX males. The motile spermatozoa, as measured by both subjective method and CASA, showed no differences between testicular sperm of XX males and stripped sperm of XY and YY males whereas testicular sperm of XY and YY males had significantly lower sperm motility. Result of protein densitometry showed similarities in protein profile between seminal plasma of XY and YY males and testicular fluids of XX males. Testis of XX males showed specific histological structures of cysts consists hypertrophied Sertoli cells.
[1] G. H. Aas, T. Refstic and B. Gjerde, "Evaluation of milt quality od Atlantic salmon". Aquaculture 95: 125-132, 1991.
[2] I. Babiak, S. Dobosz, K. Goryczko, H. Kuzminski, P. Brzuzan and S.
Ciesielski, "Androgenesis in rainbow trout using cryopreserved
spermatozoa: the effect of processing and biological factors". Theriogenology 57(4): 1229-1249, 2002b.
[3] I. Babiak, S. Dobosz, K. Goryczko, H. Kuzminski, K. Goryczko, S.
Ciesielski, P. Brzuzan, B. Urbanyi, A. Horvarth, F. Lahnsteiner and J.
Piironen, "Failure of interspecies androgenesis in salmonids". J. Fish
Biol. 61: 432-447, 2002a.
[4] K. Bieniarz, K. Goryczko, S. Dobosz and T. Grudniewski, "The effects
of 17a-methylotestosterone on rainbow trout (Oncorhynchus mykiss)".
Pol. Arch. Hydrobiol. 38: 295-301, 1991.
[5] R. Billard, "Utilisation d-un systeme tri-glycocolle pour tamponer le
dilueur d-insemination pour truite" Bulletin Français de Pêche et
Pisciculture 264: 102-112, 1977.
[6] R. Billard, J. Cosson, G. Perchec and O. Linhart, "Biology of sperm and
artificial reproduction in carp". Aquaculture 129: 95-112, 1995.
[7] V. J. Bye and R. F. Lincoln, "Commercial methods for the control of
sexual maturation in rainbow trout (Salmo gairdneri) ". Aquaculture 57:
299-310, 1986.
[8] D. Chourrout "Pressure-induced retention of second polar body and
suppression of first cleavage in rainbow trout: production of all-triploids,
all-tetraploids, and heterozygous and homozygous diploid
gynogenetics". Aquaculture 36: 111-26, 1984.
[9] A. Ciereszko and K. Dabrowski, "Estimation of sperm concentration of
rainbow trout, whitefish and yellow perch using spectrophotometric
technique". Aquaculture 109: 367-373, 1993.
[10] A. Ciereszko and K. Dabrowski, "Relationship between biochemical
constituents of fish sperm and fertility: the effect of short-term storage".
Fish Physiol Biochem. 12: 357-367, 1994
[11] A. Ciereszko, B. Piros, K. Dabrowski, D. Kucharczyk, M. J. Łuczyński,
S. Dobosz, and J. Glogowski, "Serine proteinase inhibitors of seminal
plasma of teleost fish: distribution of activity, electrophoretic profiles
and relation to proteinase inhibitors of blood". J.Fish Bio. 53: 1292-
1305, 1998.
[12] A. Ciereszko, T. Własow, S. Dobosz, K. Goryczko and J. Glogowski,
"Blood cells in rainbow trout Oncorhynchus mykiss ilt: relation to milt
collection method and sampling period". Theriogenology 62: 1353-1364,2004.
[13] G. J. Dietrich, R. Kowalski, M. Wojtczak, S. Dobosz and K. Goryczko,
"Motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa in relation to sequential collection of milt, time of post
mortem storage and anesthesia". Fish Physiol. Biochem. 31: 1-9, 2005b.
[14] G. J. Dietrich, A. Szpyrka, M. Wojtczak, S. Dobosz, K. Goryczko, Ł.
Żakowski and A. Ciereszko, „Effects of UV irradiation and hydrogen
peroxide on DNA fragmentation, motility and fertilizing ability of
rainbow trout (Oncorhynchus mykiss) spermatozoa”. Theriogenology 64:
1809-1822, 2005a
[15] E.M. Donaldson, “Manipulation of reproduction in farmed fish.” Anim
Reprod Sci 42: 381–392, 1996.
[16] C. Dreanno, M. Suquet, E. Desbruyeres, J. Cosson, H. Le Delliou, and
R. Billard, “Effect of urine on sperm quality in turbot (Psetta maxima)”.
Aquaculture 169(3–4): 247–262, 1998.
[17] K. Dziewulska and J. Domagała, “Histology of salmonid testis during
maturation”. Reprod. Biol. 3(1): 47-61, 2003.
[18] J. L. Fitzpatrick, J. C. Henry, N. R. Liley and R. H. Devlin, “Sperm
characteristicks and fertilization succes of masculinized coho salmon
(Oncorhynchus kisutch)”. Aquaculture 249: 459-468, 2005.
[19] M. J. G. Gage, C. P. Macfarlane, S. Yeates, R. G. Ward, J. B. Searle and
G. A. Parker, “Spermatozoal traits and sperm competition in Atlantic
salmon: relative sperm velocity is the primary determinant of
fertilization success”. Curr. Biol. 14: 44–47, 2004.
[20] J. F. Galas, A. Hejmej, J. Glogowski and B. Bilińska, “Morphological
and functional alterations in testis and efferent ducts of homogametic
rainbow trout Oncorhynchus mykiss Walbaum”. Ann N Y Acad Sci 1163:
398–401, 2009.
[21] A. J. Geffen and J. P. Evans, “Sperm traits and fertilization success of
male and sex-reversed female rainbow trout (Oncorhynchus mykiss)”.
Aquaculture 182: 61-72, 2000.
[22] S.E. Hartley, “The chromosomes of Salmonid fishes”. Biol. Rev. 62:
197-214, 1987.
[23] D. J. Hoysak and N. R. Liley. “Fertilization dynamics in sockeye salmon
and a comparison of sperm from alternative male phenotypes”. J. Fish
Biol. 58: 1286-1300, 2000.
[24] D. E. Kime, K. J. W. Van Look, B. G. McAllister, G. Huyskens, E.
Rurangwa and F. Ollevier , “Computer-assisted sperm analysis (CASA)
as a tool for monitoring sperm quality in fish”. Comp Biochem Physiol C
Pharmacol Toxicol Endocrinol 130: 425–433, 2001.
[25] K. Kinnberg, B. Korsgaard, P. Bjerregaard and A. Jespersen, “Effects of
nonylphenol and 17β-estradiol on vitellogenin synthesis and testis
morphology in male platyfish Xiphophorus maculates”. J. Exp. Biol.
203: 171-181, 2000.
[26] M. Koldras, M. Loir, G. Maisse and F. Le Gae, “Study of the
composition of the seminal fluid and of sperm motility along genital
tract, during a spawning season, in the rainbow trout (Oncorhynchus
mykiss) “. Aquat. Living Resour. 9(4): 337-345, 1996.
[27] F. Lahnsteiner, B. Berger, T. Weismann and R. A. Patzner,
„Physiological and biochemical determination of rainbow trout,
Oncorhynchus mykiss, sperm quality for cryopreservation”. J. Appl.
Ichthyol. 6(4): 47-73, 1996.
[28] F. Lahnsteiner, B. Berger, T. Weismann and R. A. Patzner,
„Determination of sperm quality of the rainbow trout, Oncorhynchus
mykiss, by sperm motility, seminal plasma parameters, and spermatozoal
metabolism”. Aquaculture 163: 163-181, 1998.
[29] F. Lahnsteiner, N. Mansour and B. Berger. “Seminal plasma proteins
prolong the viability of rainbow trout (Oncorynchus mykiss)
spermatozoa”. Theriogenology 62: 801-808, 2004.
[30] F. Lahnsteiner, R. A. Patzner and T. Weismann, „Energy resources of
spermatozoa of the rainbow trout Oncorhynchus mykiss (Pisces,
Teleostei)”. Reprod. Nutr. Dev. 33: 349-360, 1993b.
[31] F. Lahnsteiner, R. Patzner and T. Weismann, “The spermatic ducts of
Salmonid fishes (Salmonidae, Teleostei). Morphology, histochemistry
and composition of the secretion”. J. Fish Biol. 42: 79-93, 1993a.
[32] F. Lahnsteiner, R. Patzner and T. Weismann, “The testicular main ducts
and the spermatic ducts in some cyprinid fishes-II. Composition of the
seminal fluid”. J. Fish Biol. 44: 459-467, 1994.
[33] N. Mansour, A. Ramoun and F. Lahnsteiner, “Quality of testicular sperm
of the African catfish Clarias gariepinus (Burchell, 1822) and its
relationship with fertilization and hatching success”. Aquacult. Res. 36:
1422-1428, 2005.
[34] S. Morisawa and M. Morisawa, “Acquisition of potential for sperm
motility in rainbow trout and chum salmon”. J. Exp. Biol. 126: 89-96,
1986.
[35] H. Ohta, T. Unuma, M. Yoshioka and M. Kashiwagi, “Effects of
bicarbonate ions and pH on acquisition and maintenance of potential for
motility in ayu, Plecoglossus altivelis Temminck et Schlegel
(Osmeridae), spermatozoa”. Aquacult. Res. 32: 385-392, 2001.
[36] A. Pecio, F. Lahnsteiner and J. Rafiński, “Ultrastucture of the epithelial
cells in the aspermatogenic part of the testis in Mimagoniates barberi
(Teleostei: Characidae: Glandulocaudinae) and the role of their
secretions in spermatozeugmata formation”. Ann. Anat. 183: 427-435,
2001.
[37] G. Perchec J. , Cosson, F. Andre and R. Billard, “Degradation of the
quality of carp sperm by urine contamination during stripping”.
Aquaculture 129: 135-136, 1995.
[38] K. Ravinder, K. Nasaruddin, K. C. Majumdar and S. Shivaji,
”Computerized analysis of motility, motility patterns and motility
parameters of spermatozoa of carp following short-term storage of
sperm”. J. Fish Biol. 50: 1309-1328, 1997.
[39] H. Schagger and G. von Jagow, “Tricine-sodium dodecyl sulfate-
polyacrylamide gel electrophoresis for the separation of proteins in the
range from 1 to 100 kDa”. Anal. Biochem. 166: 368-379, 1987.
[40] A. P. Scott and S. M. Baynes, “A review of the biology, handling and
storage of salmonid spermatozoa”. J. Fish Biol. 17: 707-739, 1980.
[41] S. Shivaji, J. Peedicayil andD. L. Girija, “Analysis of the motility
parameters of in vitro hyperactivated hamster spermatozoa”. Mol.
Reprod. Dev. 42: 233-247, 1995
[1] G. H. Aas, T. Refstic and B. Gjerde, "Evaluation of milt quality od Atlantic salmon". Aquaculture 95: 125-132, 1991.
[2] I. Babiak, S. Dobosz, K. Goryczko, H. Kuzminski, P. Brzuzan and S.
Ciesielski, "Androgenesis in rainbow trout using cryopreserved
spermatozoa: the effect of processing and biological factors". Theriogenology 57(4): 1229-1249, 2002b.
[3] I. Babiak, S. Dobosz, K. Goryczko, H. Kuzminski, K. Goryczko, S.
Ciesielski, P. Brzuzan, B. Urbanyi, A. Horvarth, F. Lahnsteiner and J.
Piironen, "Failure of interspecies androgenesis in salmonids". J. Fish
Biol. 61: 432-447, 2002a.
[4] K. Bieniarz, K. Goryczko, S. Dobosz and T. Grudniewski, "The effects
of 17a-methylotestosterone on rainbow trout (Oncorhynchus mykiss)".
Pol. Arch. Hydrobiol. 38: 295-301, 1991.
[5] R. Billard, "Utilisation d-un systeme tri-glycocolle pour tamponer le
dilueur d-insemination pour truite" Bulletin Français de Pêche et
Pisciculture 264: 102-112, 1977.
[6] R. Billard, J. Cosson, G. Perchec and O. Linhart, "Biology of sperm and
artificial reproduction in carp". Aquaculture 129: 95-112, 1995.
[7] V. J. Bye and R. F. Lincoln, "Commercial methods for the control of
sexual maturation in rainbow trout (Salmo gairdneri) ". Aquaculture 57:
299-310, 1986.
[8] D. Chourrout "Pressure-induced retention of second polar body and
suppression of first cleavage in rainbow trout: production of all-triploids,
all-tetraploids, and heterozygous and homozygous diploid
gynogenetics". Aquaculture 36: 111-26, 1984.
[9] A. Ciereszko and K. Dabrowski, "Estimation of sperm concentration of
rainbow trout, whitefish and yellow perch using spectrophotometric
technique". Aquaculture 109: 367-373, 1993.
[10] A. Ciereszko and K. Dabrowski, "Relationship between biochemical
constituents of fish sperm and fertility: the effect of short-term storage".
Fish Physiol Biochem. 12: 357-367, 1994
[11] A. Ciereszko, B. Piros, K. Dabrowski, D. Kucharczyk, M. J. Łuczyński,
S. Dobosz, and J. Glogowski, "Serine proteinase inhibitors of seminal
plasma of teleost fish: distribution of activity, electrophoretic profiles
and relation to proteinase inhibitors of blood". J.Fish Bio. 53: 1292-
1305, 1998.
[12] A. Ciereszko, T. Własow, S. Dobosz, K. Goryczko and J. Glogowski,
"Blood cells in rainbow trout Oncorhynchus mykiss ilt: relation to milt
collection method and sampling period". Theriogenology 62: 1353-1364,2004.
[13] G. J. Dietrich, R. Kowalski, M. Wojtczak, S. Dobosz and K. Goryczko,
"Motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa in relation to sequential collection of milt, time of post
mortem storage and anesthesia". Fish Physiol. Biochem. 31: 1-9, 2005b.
[14] G. J. Dietrich, A. Szpyrka, M. Wojtczak, S. Dobosz, K. Goryczko, Ł.
Żakowski and A. Ciereszko, „Effects of UV irradiation and hydrogen
peroxide on DNA fragmentation, motility and fertilizing ability of
rainbow trout (Oncorhynchus mykiss) spermatozoa”. Theriogenology 64:
1809-1822, 2005a
[15] E.M. Donaldson, “Manipulation of reproduction in farmed fish.” Anim
Reprod Sci 42: 381–392, 1996.
[16] C. Dreanno, M. Suquet, E. Desbruyeres, J. Cosson, H. Le Delliou, and
R. Billard, “Effect of urine on sperm quality in turbot (Psetta maxima)”.
Aquaculture 169(3–4): 247–262, 1998.
[17] K. Dziewulska and J. Domagała, “Histology of salmonid testis during
maturation”. Reprod. Biol. 3(1): 47-61, 2003.
[18] J. L. Fitzpatrick, J. C. Henry, N. R. Liley and R. H. Devlin, “Sperm
characteristicks and fertilization succes of masculinized coho salmon
(Oncorhynchus kisutch)”. Aquaculture 249: 459-468, 2005.
[19] M. J. G. Gage, C. P. Macfarlane, S. Yeates, R. G. Ward, J. B. Searle and
G. A. Parker, “Spermatozoal traits and sperm competition in Atlantic
salmon: relative sperm velocity is the primary determinant of
fertilization success”. Curr. Biol. 14: 44–47, 2004.
[20] J. F. Galas, A. Hejmej, J. Glogowski and B. Bilińska, “Morphological
and functional alterations in testis and efferent ducts of homogametic
rainbow trout Oncorhynchus mykiss Walbaum”. Ann N Y Acad Sci 1163:
398–401, 2009.
[21] A. J. Geffen and J. P. Evans, “Sperm traits and fertilization success of
male and sex-reversed female rainbow trout (Oncorhynchus mykiss)”.
Aquaculture 182: 61-72, 2000.
[22] S.E. Hartley, “The chromosomes of Salmonid fishes”. Biol. Rev. 62:
197-214, 1987.
[23] D. J. Hoysak and N. R. Liley. “Fertilization dynamics in sockeye salmon
and a comparison of sperm from alternative male phenotypes”. J. Fish
Biol. 58: 1286-1300, 2000.
[24] D. E. Kime, K. J. W. Van Look, B. G. McAllister, G. Huyskens, E.
Rurangwa and F. Ollevier , “Computer-assisted sperm analysis (CASA)
as a tool for monitoring sperm quality in fish”. Comp Biochem Physiol C
Pharmacol Toxicol Endocrinol 130: 425–433, 2001.
[25] K. Kinnberg, B. Korsgaard, P. Bjerregaard and A. Jespersen, “Effects of
nonylphenol and 17β-estradiol on vitellogenin synthesis and testis
morphology in male platyfish Xiphophorus maculates”. J. Exp. Biol.
203: 171-181, 2000.
[26] M. Koldras, M. Loir, G. Maisse and F. Le Gae, “Study of the
composition of the seminal fluid and of sperm motility along genital
tract, during a spawning season, in the rainbow trout (Oncorhynchus
mykiss) “. Aquat. Living Resour. 9(4): 337-345, 1996.
[27] F. Lahnsteiner, B. Berger, T. Weismann and R. A. Patzner,
„Physiological and biochemical determination of rainbow trout,
Oncorhynchus mykiss, sperm quality for cryopreservation”. J. Appl.
Ichthyol. 6(4): 47-73, 1996.
[28] F. Lahnsteiner, B. Berger, T. Weismann and R. A. Patzner,
„Determination of sperm quality of the rainbow trout, Oncorhynchus
mykiss, by sperm motility, seminal plasma parameters, and spermatozoal
metabolism”. Aquaculture 163: 163-181, 1998.
[29] F. Lahnsteiner, N. Mansour and B. Berger. “Seminal plasma proteins
prolong the viability of rainbow trout (Oncorynchus mykiss)
spermatozoa”. Theriogenology 62: 801-808, 2004.
[30] F. Lahnsteiner, R. A. Patzner and T. Weismann, „Energy resources of
spermatozoa of the rainbow trout Oncorhynchus mykiss (Pisces,
Teleostei)”. Reprod. Nutr. Dev. 33: 349-360, 1993b.
[31] F. Lahnsteiner, R. Patzner and T. Weismann, “The spermatic ducts of
Salmonid fishes (Salmonidae, Teleostei). Morphology, histochemistry
and composition of the secretion”. J. Fish Biol. 42: 79-93, 1993a.
[32] F. Lahnsteiner, R. Patzner and T. Weismann, “The testicular main ducts
and the spermatic ducts in some cyprinid fishes-II. Composition of the
seminal fluid”. J. Fish Biol. 44: 459-467, 1994.
[33] N. Mansour, A. Ramoun and F. Lahnsteiner, “Quality of testicular sperm
of the African catfish Clarias gariepinus (Burchell, 1822) and its
relationship with fertilization and hatching success”. Aquacult. Res. 36:
1422-1428, 2005.
[34] S. Morisawa and M. Morisawa, “Acquisition of potential for sperm
motility in rainbow trout and chum salmon”. J. Exp. Biol. 126: 89-96,
1986.
[35] H. Ohta, T. Unuma, M. Yoshioka and M. Kashiwagi, “Effects of
bicarbonate ions and pH on acquisition and maintenance of potential for
motility in ayu, Plecoglossus altivelis Temminck et Schlegel
(Osmeridae), spermatozoa”. Aquacult. Res. 32: 385-392, 2001.
[36] A. Pecio, F. Lahnsteiner and J. Rafiński, “Ultrastucture of the epithelial
cells in the aspermatogenic part of the testis in Mimagoniates barberi
(Teleostei: Characidae: Glandulocaudinae) and the role of their
secretions in spermatozeugmata formation”. Ann. Anat. 183: 427-435,
2001.
[37] G. Perchec J. , Cosson, F. Andre and R. Billard, “Degradation of the
quality of carp sperm by urine contamination during stripping”.
Aquaculture 129: 135-136, 1995.
[38] K. Ravinder, K. Nasaruddin, K. C. Majumdar and S. Shivaji,
”Computerized analysis of motility, motility patterns and motility
parameters of spermatozoa of carp following short-term storage of
sperm”. J. Fish Biol. 50: 1309-1328, 1997.
[39] H. Schagger and G. von Jagow, “Tricine-sodium dodecyl sulfate-
polyacrylamide gel electrophoresis for the separation of proteins in the
range from 1 to 100 kDa”. Anal. Biochem. 166: 368-379, 1987.
[40] A. P. Scott and S. M. Baynes, “A review of the biology, handling and
storage of salmonid spermatozoa”. J. Fish Biol. 17: 707-739, 1980.
[41] S. Shivaji, J. Peedicayil andD. L. Girija, “Analysis of the motility
parameters of in vitro hyperactivated hamster spermatozoa”. Mol.
Reprod. Dev. 42: 233-247, 1995
@article{"International Journal of Biological, Life and Agricultural Sciences:57881", author = "Radosław K. Kowalski and Beata Sarosiek and Wiesław Demianowicz and Jędrek Judek and Krzysztof Goryczko and Stefan Dobosz and Henryk Kuźmiński and Krystyna Demska-Zakęś and Igor Babiak and Jan Glogowski", title = "Quantitative Characteristics of Rainbow Trout, Oncorhynchus Mykiss, Neo-Males (XX Genotype) and Super-Males (YY Genotype) Sperm", abstract = "Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in samples of stripped and testicular sperm obtained from XY and YY males, and testicular sperm of XX males. The motile spermatozoa, as measured by both subjective method and CASA, showed no differences between testicular sperm of XX males and stripped sperm of XY and YY males whereas testicular sperm of XY and YY males had significantly lower sperm motility. Result of protein densitometry showed similarities in protein profile between seminal plasma of XY and YY males and testicular fluids of XX males. Testis of XX males showed specific histological structures of cysts consists hypertrophied Sertoli cells.
", keywords = "fish, genotype, rainbow trout, sperm.", volume = "5", number = "5", pages = "277-8", }
