Phytotoxicity of Lead on the Physiological Parameters of Two Varieties of Broad Bean (Vicia faba)
The phytotoxicity of heavy metals can be expressed
on roots and visible part of plants and is characterized by molecular
and metabolic answers at various levels of organization of the whole
plant. The present study was undertaken on two varieties of broad
bean Vicia faba (Sidi Aïch and Super Aguadulce). The device was
mounted on a substrate prepared by mixing sand, soil and compost,
the substrate was artificially contaminated with three doses of lead
nitrate [Pb(NO3)2] 0, 500 and 1000 ppm. Our objective is to follow
the behavior of plant opposite the stress by evaluating the
physiological parameters. The results reveal a reduction in the
parameters of the productivity (chlorophyll and proteins production)
with an increase in the osmoregulators (soluble sugars and
proline).These results show that the production of broad bean is
strongly modified by the disturbance of its internal physiology under
lead exposure.
[1] A. Tremel-Schaub and I. Feix, “Contamination des sols Transferts des
sols vers les plantes,” EDP Sciences, ADEME Éditions; 2005.
[2] C. Dumat, K. Quenea, A. Bermond, S. Toinen and MF. Benedetti, “A
study of the trace metal ion influence on the turn-over of soil organic
matter in various cultivated contaminated soils," Environmental
Pollution 2006; 142: 521-529.
[3] P. Sharma and RS. Dubey, "Lead toxicity in plants," Brazilian Journal
of Plant Physiology 2005; 17(1): 35-52.
[4] J. Brunet,A. Repellin, G. Varrault, N. Terryn and Y. Zuily-Fodil, "Lead
accumulation in the roots of grass pea (Lathyrus sativus L.): a novel
plant for phytoremediation systems?," Plant biology and
pathology/Biologie et pathologie végétales. C.R. Biologies 2008; 331:
859-864.
[5] A. Piotrowska, A. Bajguz, B. Godlewska-Zylkiewicz, R. Czerpak and
M. Kaminska, "Jasmonic acid as modulator of Pb toxicity in aquatic
plant Wolffia arrhiza (Lemnaceae)," Environ Exp Bot 2009; 66(3):507-
513.
[6] R. Singh, RD. Tripathi, S. Dwivedi, A. Kumar, PK. Trivedi and D.
Chakrabarty, "Pb bioaccumulation potential of an aquatic macrophyte
Najas indica are related to antioxidant system," Bioresour Technol
2010; 101:3025-3032.
[7] HK. Lichtenthaler, "Chlorophylls and carotenoids: Pigments of
photosynthetic biomembrane," Methods Enzymology 1987; 148: 350–
381.
[8] J. Wang, W. Li, C. Zhang and S. Ke, "Physiological responses and
detoxific mechanisms to Pb, Zn, Cu and Cd in young seedlings of
Paulownia forunei," Journal of Environmental Sciences 2010; 22(12):
1916-1922.
[9] R. Shields and W. Burnett, "Determination of protein bound
carbohydrate in serum by a just modified anthrone method," Anal.
Chem 1960; 32: 885-886.
[10] J. Kjeldahl, "A new method for the determination of nitrogen in organic
matter," Zeitschreft fur Analytische Chemie 1883; 22: 366-382.
[11] C. Mathieu and F. Pieltan, "Analyse chimique des sols- Méthodes
choisis," Ed Tec & Doc, Lavoisier, Paris; 2003, p.351-376.
[12] W. Troll and J. Lindsley, "A photometric method for the determination
of proline," J.Biochem 1955; 215: 655-660.
[13] W. Dreier and M. Göring, "Der einfluss hoher salzkonzentration auf
verschieden physiollogische parameter von maiswurzeln," Wiss Z. der
Humboldt Univ Berlin, Math. Naturwiss. R 1974; 23: 641-644.
[14] W. Jiang and D. Liu, "Pb-induced cellular defense system in the root
meristematic cells of Allium sativum L.," BMC Plant Biol 2010; 10:40-
40.
[15] Z. Xiang, J. Zhao and M. Li, "Lead toxicity in Brassica pekinensis
Rupr.: Effect on nitrate assimilation and growth," Environmental
Toxicology 2006; 21(2): 147-153.
[16] B. Pourrut, "Implication du stress oxydatif dans la toxicité du plomb sur
une plante modèle, Vicia faba," Thèse de doctorat de l’université de
Toulouse 2008; 284p.
[17] AK. Hedaya, "Lead Accumulation and its Effect on Photosynthesis and
Free Amino Acids in Vicia faba Grown Hydroponically," Australian
Journal of Basic and Applied Sciences 2008; 2(3): 438-446.
[18] M. Qureshi, M. Abdin, S. Qadirand M. Iqbal, "Lead-induced oxidative
stress and metabolic alterations in Cassia angustifolia Vahl," Biologia
Plantarum 2007; 51(1): 121-128.
[19] R. John, P. Ahmad, K. Gadgiland S. Sharma, "Effect of cadmium and
lead on growth, biochemical parameters and uptake in Lemna
polyrrhiza L.," Plant Soil Environ 2008; 54 (6): 262-270.
[20] MM. Azooz, MM. Youssef and MA. Al-Omair, "Comparative
evaluation of zinc and lead and their synergistic effects on growth and
some physiological responses of Hassawi Okra (Hibiscus esculentus)
seedlings," American journal of plant physiogy 2011; 6 (6): 269-282.
[1] A. Tremel-Schaub and I. Feix, “Contamination des sols Transferts des
sols vers les plantes,” EDP Sciences, ADEME Éditions; 2005.
[2] C. Dumat, K. Quenea, A. Bermond, S. Toinen and MF. Benedetti, “A
study of the trace metal ion influence on the turn-over of soil organic
matter in various cultivated contaminated soils," Environmental
Pollution 2006; 142: 521-529.
[3] P. Sharma and RS. Dubey, "Lead toxicity in plants," Brazilian Journal
of Plant Physiology 2005; 17(1): 35-52.
[4] J. Brunet,A. Repellin, G. Varrault, N. Terryn and Y. Zuily-Fodil, "Lead
accumulation in the roots of grass pea (Lathyrus sativus L.): a novel
plant for phytoremediation systems?," Plant biology and
pathology/Biologie et pathologie végétales. C.R. Biologies 2008; 331:
859-864.
[5] A. Piotrowska, A. Bajguz, B. Godlewska-Zylkiewicz, R. Czerpak and
M. Kaminska, "Jasmonic acid as modulator of Pb toxicity in aquatic
plant Wolffia arrhiza (Lemnaceae)," Environ Exp Bot 2009; 66(3):507-
513.
[6] R. Singh, RD. Tripathi, S. Dwivedi, A. Kumar, PK. Trivedi and D.
Chakrabarty, "Pb bioaccumulation potential of an aquatic macrophyte
Najas indica are related to antioxidant system," Bioresour Technol
2010; 101:3025-3032.
[7] HK. Lichtenthaler, "Chlorophylls and carotenoids: Pigments of
photosynthetic biomembrane," Methods Enzymology 1987; 148: 350–
381.
[8] J. Wang, W. Li, C. Zhang and S. Ke, "Physiological responses and
detoxific mechanisms to Pb, Zn, Cu and Cd in young seedlings of
Paulownia forunei," Journal of Environmental Sciences 2010; 22(12):
1916-1922.
[9] R. Shields and W. Burnett, "Determination of protein bound
carbohydrate in serum by a just modified anthrone method," Anal.
Chem 1960; 32: 885-886.
[10] J. Kjeldahl, "A new method for the determination of nitrogen in organic
matter," Zeitschreft fur Analytische Chemie 1883; 22: 366-382.
[11] C. Mathieu and F. Pieltan, "Analyse chimique des sols- Méthodes
choisis," Ed Tec & Doc, Lavoisier, Paris; 2003, p.351-376.
[12] W. Troll and J. Lindsley, "A photometric method for the determination
of proline," J.Biochem 1955; 215: 655-660.
[13] W. Dreier and M. Göring, "Der einfluss hoher salzkonzentration auf
verschieden physiollogische parameter von maiswurzeln," Wiss Z. der
Humboldt Univ Berlin, Math. Naturwiss. R 1974; 23: 641-644.
[14] W. Jiang and D. Liu, "Pb-induced cellular defense system in the root
meristematic cells of Allium sativum L.," BMC Plant Biol 2010; 10:40-
40.
[15] Z. Xiang, J. Zhao and M. Li, "Lead toxicity in Brassica pekinensis
Rupr.: Effect on nitrate assimilation and growth," Environmental
Toxicology 2006; 21(2): 147-153.
[16] B. Pourrut, "Implication du stress oxydatif dans la toxicité du plomb sur
une plante modèle, Vicia faba," Thèse de doctorat de l’université de
Toulouse 2008; 284p.
[17] AK. Hedaya, "Lead Accumulation and its Effect on Photosynthesis and
Free Amino Acids in Vicia faba Grown Hydroponically," Australian
Journal of Basic and Applied Sciences 2008; 2(3): 438-446.
[18] M. Qureshi, M. Abdin, S. Qadirand M. Iqbal, "Lead-induced oxidative
stress and metabolic alterations in Cassia angustifolia Vahl," Biologia
Plantarum 2007; 51(1): 121-128.
[19] R. John, P. Ahmad, K. Gadgiland S. Sharma, "Effect of cadmium and
lead on growth, biochemical parameters and uptake in Lemna
polyrrhiza L.," Plant Soil Environ 2008; 54 (6): 262-270.
[20] MM. Azooz, MM. Youssef and MA. Al-Omair, "Comparative
evaluation of zinc and lead and their synergistic effects on growth and
some physiological responses of Hassawi Okra (Hibiscus esculentus)
seedlings," American journal of plant physiogy 2011; 6 (6): 269-282.
@article{"International Journal of Biological, Life and Agricultural Sciences:70708", author = "El H. Bouziani and H. A. Reguieg Yssaad", title = "Phytotoxicity of Lead on the Physiological Parameters of Two Varieties of Broad Bean (Vicia faba)", abstract = "The phytotoxicity of heavy metals can be expressed
on roots and visible part of plants and is characterized by molecular
and metabolic answers at various levels of organization of the whole
plant. The present study was undertaken on two varieties of broad
bean Vicia faba (Sidi Aïch and Super Aguadulce). The device was
mounted on a substrate prepared by mixing sand, soil and compost,
the substrate was artificially contaminated with three doses of lead
nitrate [Pb(NO3)2] 0, 500 and 1000 ppm. Our objective is to follow
the behavior of plant opposite the stress by evaluating the
physiological parameters. The results reveal a reduction in the
parameters of the productivity (chlorophyll and proteins production)
with an increase in the osmoregulators (soluble sugars and
proline).These results show that the production of broad bean is
strongly modified by the disturbance of its internal physiology under
lead exposure.", keywords = "Broad bean, lead, stress, physiological parameters,
phytotoxicity.", volume = "9", number = "8", pages = "942-4", }
