Overview of CARDIOSENSOR Project on the Development of a Nanosensor for Assessing the Risk of Cardiovascular Disease
This paper aims at overviewing the topics of a research project (CARDIOSENSOR) on the field of health sciences (biomaterials and biomedical engineering). The project has focused on the development of a nanosensor for the assessment of the risk of cardiovascular diseases by the monitoring of C-reactive protein (CRP), which has been currently considered as the best validated inflammatory biomarker associated to cardiovascular diseases. The project involves tasks such as: 1) the development of sensor devices based on field effect transistors (FET): assembly, optimization and validation; 2) application of sensors to the detection of CRP in standard solutions and comparison with enzyme-linked immunosorbent assay (ELISA); and 3) application of sensors to real samples such as blood and saliva and evaluation of their ability to predict the risk of cardiovascular disease.
[1] S. Allender, P. Scarborough, V. Peto, M. Rayner, J. Leal, R. Luengo
Fernandez, A. Gray, "European cardiovascular disease statistics - 2008”, Health Econ., vol. 2008, p. 1-112, 2008.
[2] V.L. Roger, A.S. Go, D.M. Lloyd-Jones, E.J. Benjamin, J.D. Berry, W.B. Borden, D.M. Bravata, S. Dai, E.S. Ford, C.S. Fox, H.J. Fullerton, C. Gillespie, S.M. Hailpern, J.A. Heit, V.J. Howard, B.M. Kissela, S.J.
Kittner, D.T. Lackland, J.H. Lichtman, L.D. Lisabeth, D.M. Makuc,
G.M. Marcus, A. Marelli, D.B. Matchar, C.S. Moy, D. Mozaffarian,
M.E. Mussolino, G. Nichol, N.P. Paynter, E.Z. Soliman, P.D. Sorlie, N.
Sotoodehnia, T.N. Turan, S.S. Virani, N.D.Wong, D. Woo, M.B. Turner,
"Heart disease and stroke statistics - 2012 update. A report from the
American Heart Association”, Circulation, vol. 125, p. 2-220, 2012.
[3] P.M. Ridker, "Clinical application of C-reactive protein for
cardiovascular disease detection and prevention”, Circulation, vol. 107,
p. 363-369, 2003.
[4] G.K. Hansson, "Inflammation, atherosclerosis, and coronary artery
disease”, N. Eng. J. Med., vol. 352, p. 1685-1695, 2005.
[5] T. Vo-Dinh, B. Cullum, "Biosensors and biochips: advances in
biological and medical diagnostics”, Fresen. J. Anal. Chem., vol. 366, p.
540-551, 2000.
[6] O. Rogowski, Y. Vered, I. Shapira, M. Hirsh, V. Zakut, S. Berliner,
"Introducing the wide range C-reactive protein (wr-CRP) into clinical
use for the detection of microinflammation”, Clin. Chim. Acta, vol. 358,
p. 151-158, 2005.
[7] S.-C. Shiesh, T.-C. Chou, X.-Z. Lin, P.-C. Kao, "Determination of Creactive
protein with an ultra-sensitivity immunochemiluminometric
assay”, J. Immunol. Methods, vol. 311, p. 87-95, 2006.
[8] M.H.F. Meyer, M. Hartmann, H.J. Krause, G. Blankenstein, B. Mueller-
Chorus, J. Oster, P. Miethe, M. Keusgen, "CRP determination based on
a novel magnetic biosensor”, Biosens. Bioelectron., vol. 22, p. 973-979,
2007.
[9] S. Centi, L.B. Sanmartin, S. Tombelli, I. Palchetti, M. Mascini, "
Detection of C-reactive protein (CRP) in serum by an electrochemical
aptamer-based sandwich assay”, Electroanalysis, vol. 21, p. 1309-1315,
2009.
[10] N. Kim, D.-K. Kim, Y.-J. Cho, "Development of indirect competitive
quartz crystal microbalance immunosensor for C-reactive protein”, Sens.
Actuators B, vol. 143, p. 444-448, 2009.
[11] Y.-N. Yang, H.-I. Lin, J.-H. Wang, S.-C. Shiesh, G.-B. Lee, "An
integrated microfluidic system for C-reactive protein measurement”,
Biosens. Bioelectron., vol. 24, p. 3091-3096, 2009.
[12] A. Quershi, J.H. Niazi, S. Kallempudi, Y. Gurbuz, "Label-free
capacitive biosensor for sensitive detection of multiple biomarkers using
gold interdigitated capacitor arrays”, Biosens. Bioelectron., vol. 25, p.
2318-2323, 2010.
[13] M.T. Gomes, T.A.P. Rocha, A.C. Duarte, J.A.B.P. Oliveira, "The
performance of a tetramethylammonium fluoride tetrahydrated coated
piezoelectric crystal for carbon dioxide detection”, Anal. Chim. Acta,
vol. 355, p. 235-238, 1996.
[14] T.A.P. Rocha, M.T. Gomes, A.C. Duarte, J.A.B.P. Oliveira, "Quartz
crystal microbalance with gold electrodes as a sensor for monitoring gasphase
adsorption/desorption of short chain alkylthiol and alkyl sulfides”,
Anal. Commun., vol. 35, p. 415-416, 1998.
[15] L.I.B. Silva, T.A.P. Rocha-Santos, A.C. Duarte, "Development of a
fluorosiloxane polymer coated optical fibre sensor for detection of
organic volatile compounds”, Sens. Actuators B., vol. 132, p. 280-289,
2008.
[16] L.I.B. Silva, Rocha-Santos T.A.P., Duarte A.C., "Comparison of a gas
chromatography-optical fibre (GC-OF) detector with a gas
chromatography-flame ionization detector (GC-FID) for determination
of alcoholic compounds in industrial atmospheres”, Talanta, vol. 76, p.
395-399, 2008.
[17] L.I.B. Silva, T.A.P. Rocha-Santos, A.C. Duarte, "Sensing of volatile
organic compounds in indoor atmosphere and confined areas of
industrial environments”. Global NEST Journal, vol. 10, 217-225, 2008.
[18] L.I.B. Silva, F.D.P. Ferreira, T.A.P. Rocha-Santos, A.C. Duarte,
"Carbon nanotube field-effect transistor detector associated to gas
chromatography for speciation of benzene, toluene, ethylbenzene, (o-,
m- and p-)xylene”, J. Chromatography A, vol. 1216, p. 6517-6521,
2009.
[19] C.I.L. Justino, T.A.P. Rocha-Santos, A.C. Duarte, "Sampling and
characterization of nanoaerosols in different environments”. Trends
Anal. Chem., vol. 30, p. 554-567, 2011.
[20] L.I.B. Silva, C.I.L. Justino, I. Lopes, R. Pereira, A.C. Freitas, R. Calado,
T.A.P. Rocha-Santos, T.S.L. Panteleitchouk, M.E. Pereira, A.C. Duarte,
"Optical fiber based methodology for assessment of thiocyanate in
seawater” J. Environ. Monitor., vol. 13, 6, 1811-1815, 2011.
[21] C.I.L. Justino, A.C. Freitas, T.A.P. Rocha-Santos, A.C. Duarte,
"Screening of single-walled carbon nanotubes by optical fiber sensing”,
Talanta, vol. 89, p. 105-108, 2012.
[22] M.T. Gomes, T.A. Rocha, A.C. Duarte, J.A.B.P. Oliveira,
"Determination of sulfur dioxide in wine using a quartz crystal
microbalance”. Anal. Chem., vol. 68, p. 1561-1564, 1996.
[23] L.I.B. Silva, F.D.P. Ferreira, A.C. Freitas, T.A.P. Rocha-Santos, A.C.
Duarte, "Optical fiber-based micro-analyzer for indirect measurements
of volatile amines levels in fish”. Food Chem., vol. 123, p. 806–813,
2010.
[24] L.I.B. Silva, D. Rodrigues, A.C. Freitas, A.M. Gomes, T.A.P. Rocha-
Santos, M.E. Pereira, A.C. Duarte, "Optical fibre based methodology for
screening the effect of probiotic bacteria on conjugated linoleic acid
(CLA) in curdled milk”. Food Chem., vol. 127, p. 222-227, 2011.
[25] L.I.B. Silva, F.D.P. Ferreira, A.C. Freitas, T.A.P. Rocha-Santos, A.C.
Duarte, "Optical fiber biosensor coupled to chromatographic separation
for screening of dopamine, norepinephrine and epinephrine in human
urine and plasma”, Talanta, vol. 80, p. 853-857, 2009.
[26] F.D.P. Ferreira, L.I.B. Silva, A.C. Freitas, T.A.P. Rocha-Santos, A.C.
Duarte, "High performance liquid chromatography coupled to an optical
fibre detector coated with laccase for screening catecholamines in
plasma and urine”. J. Chromatography A., vol. 1216, p. 7049-7054,
2009.
[27] C.I.L. Justino, T.A.P. Rocha-Santos, A.C. Duarte, "Review of analytical
figures of merit of sensors and biosensors in clinical applications”,
Trends Anal. Chem., vol. 29, p. 1172-1183, 2010.
[28] L.I.B. Silva, A.C. Freitas, T.A.P. Rocha-Santos, M.E. Pereira, A.C.
Duarte, "Breath analysis by optical fiber sensor for the determination of
exhaled organic compounds with a view to diagnostics”. Talanta, vol.
83, p. 1586-1594, 2011.
[29] L.I.B. Silva, A.M. Gomes, M.M. Pintado, H. Pinheiro, D. Moura, A.C.
Freitas, T.A.P. Rocha-Santos, M.E. Pereira, A.C. Duarte, "Optical fiber
bio-analyzer based on enzymatic coating matrix for catecholamines and
their metabolites assessment in patients with Down syndrome”. IEEE
Sensors J., vol. 12, p. 76-84, 2012.
[1] S. Allender, P. Scarborough, V. Peto, M. Rayner, J. Leal, R. Luengo
Fernandez, A. Gray, "European cardiovascular disease statistics - 2008”, Health Econ., vol. 2008, p. 1-112, 2008.
[2] V.L. Roger, A.S. Go, D.M. Lloyd-Jones, E.J. Benjamin, J.D. Berry, W.B. Borden, D.M. Bravata, S. Dai, E.S. Ford, C.S. Fox, H.J. Fullerton, C. Gillespie, S.M. Hailpern, J.A. Heit, V.J. Howard, B.M. Kissela, S.J.
Kittner, D.T. Lackland, J.H. Lichtman, L.D. Lisabeth, D.M. Makuc,
G.M. Marcus, A. Marelli, D.B. Matchar, C.S. Moy, D. Mozaffarian,
M.E. Mussolino, G. Nichol, N.P. Paynter, E.Z. Soliman, P.D. Sorlie, N.
Sotoodehnia, T.N. Turan, S.S. Virani, N.D.Wong, D. Woo, M.B. Turner,
"Heart disease and stroke statistics - 2012 update. A report from the
American Heart Association”, Circulation, vol. 125, p. 2-220, 2012.
[3] P.M. Ridker, "Clinical application of C-reactive protein for
cardiovascular disease detection and prevention”, Circulation, vol. 107,
p. 363-369, 2003.
[4] G.K. Hansson, "Inflammation, atherosclerosis, and coronary artery
disease”, N. Eng. J. Med., vol. 352, p. 1685-1695, 2005.
[5] T. Vo-Dinh, B. Cullum, "Biosensors and biochips: advances in
biological and medical diagnostics”, Fresen. J. Anal. Chem., vol. 366, p.
540-551, 2000.
[6] O. Rogowski, Y. Vered, I. Shapira, M. Hirsh, V. Zakut, S. Berliner,
"Introducing the wide range C-reactive protein (wr-CRP) into clinical
use for the detection of microinflammation”, Clin. Chim. Acta, vol. 358,
p. 151-158, 2005.
[7] S.-C. Shiesh, T.-C. Chou, X.-Z. Lin, P.-C. Kao, "Determination of Creactive
protein with an ultra-sensitivity immunochemiluminometric
assay”, J. Immunol. Methods, vol. 311, p. 87-95, 2006.
[8] M.H.F. Meyer, M. Hartmann, H.J. Krause, G. Blankenstein, B. Mueller-
Chorus, J. Oster, P. Miethe, M. Keusgen, "CRP determination based on
a novel magnetic biosensor”, Biosens. Bioelectron., vol. 22, p. 973-979,
2007.
[9] S. Centi, L.B. Sanmartin, S. Tombelli, I. Palchetti, M. Mascini, "
Detection of C-reactive protein (CRP) in serum by an electrochemical
aptamer-based sandwich assay”, Electroanalysis, vol. 21, p. 1309-1315,
2009.
[10] N. Kim, D.-K. Kim, Y.-J. Cho, "Development of indirect competitive
quartz crystal microbalance immunosensor for C-reactive protein”, Sens.
Actuators B, vol. 143, p. 444-448, 2009.
[11] Y.-N. Yang, H.-I. Lin, J.-H. Wang, S.-C. Shiesh, G.-B. Lee, "An
integrated microfluidic system for C-reactive protein measurement”,
Biosens. Bioelectron., vol. 24, p. 3091-3096, 2009.
[12] A. Quershi, J.H. Niazi, S. Kallempudi, Y. Gurbuz, "Label-free
capacitive biosensor for sensitive detection of multiple biomarkers using
gold interdigitated capacitor arrays”, Biosens. Bioelectron., vol. 25, p.
2318-2323, 2010.
[13] M.T. Gomes, T.A.P. Rocha, A.C. Duarte, J.A.B.P. Oliveira, "The
performance of a tetramethylammonium fluoride tetrahydrated coated
piezoelectric crystal for carbon dioxide detection”, Anal. Chim. Acta,
vol. 355, p. 235-238, 1996.
[14] T.A.P. Rocha, M.T. Gomes, A.C. Duarte, J.A.B.P. Oliveira, "Quartz
crystal microbalance with gold electrodes as a sensor for monitoring gasphase
adsorption/desorption of short chain alkylthiol and alkyl sulfides”,
Anal. Commun., vol. 35, p. 415-416, 1998.
[15] L.I.B. Silva, T.A.P. Rocha-Santos, A.C. Duarte, "Development of a
fluorosiloxane polymer coated optical fibre sensor for detection of
organic volatile compounds”, Sens. Actuators B., vol. 132, p. 280-289,
2008.
[16] L.I.B. Silva, Rocha-Santos T.A.P., Duarte A.C., "Comparison of a gas
chromatography-optical fibre (GC-OF) detector with a gas
chromatography-flame ionization detector (GC-FID) for determination
of alcoholic compounds in industrial atmospheres”, Talanta, vol. 76, p.
395-399, 2008.
[17] L.I.B. Silva, T.A.P. Rocha-Santos, A.C. Duarte, "Sensing of volatile
organic compounds in indoor atmosphere and confined areas of
industrial environments”. Global NEST Journal, vol. 10, 217-225, 2008.
[18] L.I.B. Silva, F.D.P. Ferreira, T.A.P. Rocha-Santos, A.C. Duarte,
"Carbon nanotube field-effect transistor detector associated to gas
chromatography for speciation of benzene, toluene, ethylbenzene, (o-,
m- and p-)xylene”, J. Chromatography A, vol. 1216, p. 6517-6521,
2009.
[19] C.I.L. Justino, T.A.P. Rocha-Santos, A.C. Duarte, "Sampling and
characterization of nanoaerosols in different environments”. Trends
Anal. Chem., vol. 30, p. 554-567, 2011.
[20] L.I.B. Silva, C.I.L. Justino, I. Lopes, R. Pereira, A.C. Freitas, R. Calado,
T.A.P. Rocha-Santos, T.S.L. Panteleitchouk, M.E. Pereira, A.C. Duarte,
"Optical fiber based methodology for assessment of thiocyanate in
seawater” J. Environ. Monitor., vol. 13, 6, 1811-1815, 2011.
[21] C.I.L. Justino, A.C. Freitas, T.A.P. Rocha-Santos, A.C. Duarte,
"Screening of single-walled carbon nanotubes by optical fiber sensing”,
Talanta, vol. 89, p. 105-108, 2012.
[22] M.T. Gomes, T.A. Rocha, A.C. Duarte, J.A.B.P. Oliveira,
"Determination of sulfur dioxide in wine using a quartz crystal
microbalance”. Anal. Chem., vol. 68, p. 1561-1564, 1996.
[23] L.I.B. Silva, F.D.P. Ferreira, A.C. Freitas, T.A.P. Rocha-Santos, A.C.
Duarte, "Optical fiber-based micro-analyzer for indirect measurements
of volatile amines levels in fish”. Food Chem., vol. 123, p. 806–813,
2010.
[24] L.I.B. Silva, D. Rodrigues, A.C. Freitas, A.M. Gomes, T.A.P. Rocha-
Santos, M.E. Pereira, A.C. Duarte, "Optical fibre based methodology for
screening the effect of probiotic bacteria on conjugated linoleic acid
(CLA) in curdled milk”. Food Chem., vol. 127, p. 222-227, 2011.
[25] L.I.B. Silva, F.D.P. Ferreira, A.C. Freitas, T.A.P. Rocha-Santos, A.C.
Duarte, "Optical fiber biosensor coupled to chromatographic separation
for screening of dopamine, norepinephrine and epinephrine in human
urine and plasma”, Talanta, vol. 80, p. 853-857, 2009.
[26] F.D.P. Ferreira, L.I.B. Silva, A.C. Freitas, T.A.P. Rocha-Santos, A.C.
Duarte, "High performance liquid chromatography coupled to an optical
fibre detector coated with laccase for screening catecholamines in
plasma and urine”. J. Chromatography A., vol. 1216, p. 7049-7054,
2009.
[27] C.I.L. Justino, T.A.P. Rocha-Santos, A.C. Duarte, "Review of analytical
figures of merit of sensors and biosensors in clinical applications”,
Trends Anal. Chem., vol. 29, p. 1172-1183, 2010.
[28] L.I.B. Silva, A.C. Freitas, T.A.P. Rocha-Santos, M.E. Pereira, A.C.
Duarte, "Breath analysis by optical fiber sensor for the determination of
exhaled organic compounds with a view to diagnostics”. Talanta, vol.
83, p. 1586-1594, 2011.
[29] L.I.B. Silva, A.M. Gomes, M.M. Pintado, H. Pinheiro, D. Moura, A.C.
Freitas, T.A.P. Rocha-Santos, M.E. Pereira, A.C. Duarte, "Optical fiber
bio-analyzer based on enzymatic coating matrix for catecholamines and
their metabolites assessment in patients with Down syndrome”. IEEE
Sensors J., vol. 12, p. 76-84, 2012.
@article{"International Journal of Medical, Medicine and Health Sciences:54582", author = "A.C. Duarte and C.I.L. Justino and K. Duarte and A.C. Freitas and R. Pereira and P. Chaves and P. Bettencourt and S. Cardoso and T.A.P. Rocha-Santos", title = "Overview of CARDIOSENSOR Project on the Development of a Nanosensor for Assessing the Risk of Cardiovascular Disease", abstract = "This paper aims at overviewing the topics of a research project (CARDIOSENSOR) on the field of health sciences (biomaterials and biomedical engineering). The project has focused on the development of a nanosensor for the assessment of the risk of cardiovascular diseases by the monitoring of C-reactive protein (CRP), which has been currently considered as the best validated inflammatory biomarker associated to cardiovascular diseases. The project involves tasks such as: 1) the development of sensor devices based on field effect transistors (FET): assembly, optimization and validation; 2) application of sensors to the detection of CRP in standard solutions and comparison with enzyme-linked immunosorbent assay (ELISA); and 3) application of sensors to real samples such as blood and saliva and evaluation of their ability to predict the risk of cardiovascular disease.
", keywords = "Carbon nanotubes field effect transistors, cardiovascular diseases, C-reactive protein, sensor.", volume = "7", number = "1", pages = "45-5", }
