Optimization of Two-Stage Pretreatment Combined with Microwave Radiation Using Response Surface Methodology
Pretreatment is an essential step in the conversion of
lignocellulosic biomass to fermentable sugar that used for biobutanol
production. Among pretreatment processes, microwave is considered
to improve pretreatment efficiency due to its high heating efficiency,
easy operation, and easily to combine with chemical reaction. The
main objectives of this work are to investigate the feasibility of
microwave pretreatment to enhance enzymatic hydrolysis of
corncobs and to determine the optimal conditions using response
surface methodology. Corncobs were pretreated via two-stage
pretreatment in dilute sodium hydroxide (2 %) followed by dilute
sulfuric acid 1 %. Pretreated corncobs were subjected to enzymatic
hydrolysis to produce reducing sugar. Statistical experimental design
was used to optimize pretreatment parameters including temperature,
residence time and solid-to-liquid ratio to achieve the highest amount
of glucose. The results revealed that solid-to-liquid ratio and
temperature had a significant effect on the amount of glucose.
[1] Rubin, E. (2008). Genomics of cellulosic biofuels. Nature, 454, 841-
845.
[2] Nigam, P., and Singh, A. (2011). Production of liquid biofuels from
renewable resources. Progress in Energy and Combustion Science, 37,
52-68.
[3] Han, M., Moon, S., Kim, Y., Kim, Y., Chung, B., and Choi, G. (2009).
Bioethanol production from ammonia percolated wheat straw.
Biotechnology Bioprocess Engineering, 14, 606-611.
[4] Silverstein, R., Chen, Y., Sharma, R., Boyette, M., and Osborne, J.
(2008). A comparison of chemical pretreatment methods for improving
saccharification of cotton stalks. Bioresource Technology, 98, 3000-
3011.
[5] Clark, D. and Sutton, W. (1996). Microwave Processing of Materials.
Annual Review of Materials Science, 26, 299-331.
[6] Yoo, C., Lee, C., and Kim, T. (2011). Optimization of two-stage
fractionation process for lignocellulosic biomass using response surface
methodology (RSM). Biomass & Bioenergy, 35, 4901-4909.
[7] Cara, C., Moya, M., Ballesteros, I., Negro, M.J., González, A., and Ruiz,
E. (2007). Influence of solid loading on enzymatic hydrolysis of steam
exploded or liquid hot water pretreated olive tree biomass. Process
Biochemistry, 42, 1003-1009.
[8] Kim, T.H., and Lee, Y.Y. (2007). Pretreatment of corn stover by
soaking in aqueous ammonia at moderate temperatures. Applied
Biochemistry and Biotechnology, 136-140, 81-92.
[9] Redding, A., Wang, Z., Keshwani, D., and Cheng, J. (2011). High
temperature dilute acid pretreatment of coastal Bermuda grass for
enzymatic hydrolysis. Bioresource Technology, 102, 1415-1424.
[10] Binod, P., Satyanagalakshmi, K., Sindhu, R., Janu, K.U., Sukumaran,
R.K., and Pandey, A. (2012). Short duration microwave assisted
pretreatment enhances the enzymatic saccharification and fermentable
sugar yield from sugarcane bagasse. Renewable Energy, 39, 109-116.
[11] Chen, W.H., Tu, Y.J., and Sheen, H.K. (2011). Disruption of sugarcane
bagasse lignocellulosic structure by means of dilute sulfuric acid
pretreatment with microwave-assisted heating. Applied Energy, 88,
2726-2734.
[1] Rubin, E. (2008). Genomics of cellulosic biofuels. Nature, 454, 841-
845.
[2] Nigam, P., and Singh, A. (2011). Production of liquid biofuels from
renewable resources. Progress in Energy and Combustion Science, 37,
52-68.
[3] Han, M., Moon, S., Kim, Y., Kim, Y., Chung, B., and Choi, G. (2009).
Bioethanol production from ammonia percolated wheat straw.
Biotechnology Bioprocess Engineering, 14, 606-611.
[4] Silverstein, R., Chen, Y., Sharma, R., Boyette, M., and Osborne, J.
(2008). A comparison of chemical pretreatment methods for improving
saccharification of cotton stalks. Bioresource Technology, 98, 3000-
3011.
[5] Clark, D. and Sutton, W. (1996). Microwave Processing of Materials.
Annual Review of Materials Science, 26, 299-331.
[6] Yoo, C., Lee, C., and Kim, T. (2011). Optimization of two-stage
fractionation process for lignocellulosic biomass using response surface
methodology (RSM). Biomass & Bioenergy, 35, 4901-4909.
[7] Cara, C., Moya, M., Ballesteros, I., Negro, M.J., González, A., and Ruiz,
E. (2007). Influence of solid loading on enzymatic hydrolysis of steam
exploded or liquid hot water pretreated olive tree biomass. Process
Biochemistry, 42, 1003-1009.
[8] Kim, T.H., and Lee, Y.Y. (2007). Pretreatment of corn stover by
soaking in aqueous ammonia at moderate temperatures. Applied
Biochemistry and Biotechnology, 136-140, 81-92.
[9] Redding, A., Wang, Z., Keshwani, D., and Cheng, J. (2011). High
temperature dilute acid pretreatment of coastal Bermuda grass for
enzymatic hydrolysis. Bioresource Technology, 102, 1415-1424.
[10] Binod, P., Satyanagalakshmi, K., Sindhu, R., Janu, K.U., Sukumaran,
R.K., and Pandey, A. (2012). Short duration microwave assisted
pretreatment enhances the enzymatic saccharification and fermentable
sugar yield from sugarcane bagasse. Renewable Energy, 39, 109-116.
[11] Chen, W.H., Tu, Y.J., and Sheen, H.K. (2011). Disruption of sugarcane
bagasse lignocellulosic structure by means of dilute sulfuric acid
pretreatment with microwave-assisted heating. Applied Energy, 88,
2726-2734.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:62861", author = "Jidapa Manaso and Apanee Luengnaruemitchai and Sujitra Wongkasemjit", title = "Optimization of Two-Stage Pretreatment Combined with Microwave Radiation Using Response Surface Methodology", abstract = "Pretreatment is an essential step in the conversion of
lignocellulosic biomass to fermentable sugar that used for biobutanol
production. Among pretreatment processes, microwave is considered
to improve pretreatment efficiency due to its high heating efficiency,
easy operation, and easily to combine with chemical reaction. The
main objectives of this work are to investigate the feasibility of
microwave pretreatment to enhance enzymatic hydrolysis of
corncobs and to determine the optimal conditions using response
surface methodology. Corncobs were pretreated via two-stage
pretreatment in dilute sodium hydroxide (2 %) followed by dilute
sulfuric acid 1 %. Pretreated corncobs were subjected to enzymatic
hydrolysis to produce reducing sugar. Statistical experimental design
was used to optimize pretreatment parameters including temperature,
residence time and solid-to-liquid ratio to achieve the highest amount
of glucose. The results revealed that solid-to-liquid ratio and
temperature had a significant effect on the amount of glucose.", keywords = "Corncobs, Microwave radiation, Pretreatment,
Response Surface Methodology.", volume = "7", number = "4", pages = "212-5", }