Kinetics Study for the Recombinant Cellulosome to the Degradation of Chlorella Cell Residuals
In this study, lipid-deprived residuals of microalgae
were hydrolyzed for the production of reducing sugars by using the
recombinant Bacillus cellulosome, carrying eight genes from the
Clostridium thermocellum ATCC27405. The obtained cellulosome
was found to exist mostly in the broth supernatant with a cellulosome
activity of 2.4 U/mL. Furthermore, the Michaelis-Menten constant
(Km) and Vmax of cellulosome were found to be 14.832 g/L and 3.522
U/mL. The activation energy of the cellulosome to hydrolyze
microalgae LDRs was calculated as 32.804 kJ/mol.
[1] W. Han, W. Clarke, S. Pratt, "Composting of waste algae: A review,"
Waste Manage., vol. 34, pp. 1148-1155, 2014.
[2] C. C. Lin, C. H. Wei, C. I. Chen, C. J. Shieh, Y. C. Liu, "Characteristics of
the photosynthesis microbial fuel cell with a Spirulina platensis biofilm,"
Bioresource Technol., vol. 135, pp. 640-643, 2013.
[3] C. C. Fu, T. C. Hung, J. Y. Chen, C. H. Su, W. T. Wu, "Hydrolysis of
microalgae cell walls for production of reducing sugar and lipid
extraction," Bioresource Technol., vol. 101, pp. 8750-8754, 2010.
[4] Y. Chisti, "Biodiesel from microalgae," Biotechnol. Adv., vol. 25, pp.
294-306, 2007.
[5] C. H. Hsieh, W. T. Wu, "Cultivation of microalgae for oil production with
a cultivation strategy of urea limitation," Bioresource Technol., vol. 100,
pp. 3921-3926, 2009.
[6] E. Sanchez, K. Ojeda, M. El-Halwagi, V. Kafarov, "Biodiesel from
microalgae oil production in two sequential esterification/
transesterification reactors: Pinch analysis of heat integration," Chem.
Eng. J., vol. 176, pp. 211-216, 2011.
[7] Y. M. Dai, K. T. Chen, C. C. Chen, "Study of the microwave lipid
extraction from microalgae for biodiesel production," Chem. Eng. J., vol.
250, pp. 267-273, 2014.
[8] I. Rawat, R. R. Kumar, T. Mutanda, F. Bux, Appl. Energ. pp. 444-467.
2013,
[9] H. G. Gerken, B. Donohoe, E. P. Knoshaug, "Enzymatic cell wall
degradation of Chlorella vulgaris and other microalgae for biofuels
production," Planta, vol. 237, pp. 239-253, 2013
[10] M. Morweiser, O. Kruse, B. Hankamer, C. Posten, "Developments and
perspectives of photobioreactors for biofuel production," Appl.
Microbiol. Biotechnol., vol. 87, pp. 1291-1301, 2010.
[11] M. L. Ghirardi, J. P. Zhang, J. W. Lee, T. Flynn, et al., "Microalgae: a
green source of renewable H-2," Trends Biotechnol., vol. 18, pp. 506-511,
2000.
[12] J. B. Holm-Nielsen, T. Al Seadi, P. Oleskowicz-Popiel, "The future of
anaerobic digestion and biogas utilization," Bioresource Technol., vol.
100, pp. 5478-5484, 2009 [13] A. Vergara-Fernandez, G. Vargas, N. Alarcon, A. Velasco, "Evaluation of
marine algae as a source of biogas in a two-stage anaerobic reactor
system," Biomass Bioenerg., vol. 32, pp. 338-344, 2008.
[14] C. Y. Chen, M. D. Bai, J. S. Chang, "Improving microalgal oil collecting
efficiency by pretreating the microalgal cell wall with destructive
bacteria," Biochem. Eng. J., vol. 81, pp. 170-176, 2013.
[15] M. Girfoglio, M. Rossi, R. Cannio, "Cellulose Degradation by Sulfolobus
solfataricus Requires a Cell-Anchored Endo-beta-1-4-Glucanase," J.
Bacteriol., vol. 194, pp. 5091-5100, 2012.
[16] M. K. Bhat, "Cellulases and related enzymes in biotechnology,"
Biotechnol. Adv., vol. 18, pp. 355-383, 2000.
[17] W. H. Schwarz, "The cellulosome and cellulose degradation by anaerobic
bacteria," Appl. Microbiol. Biotechnol., vol. 56, pp. 634-649, 2001.
[18] C. Y. Ho, J. J. Chang, S. C. Lee, T. Y. Chin, et al., "Development of
cellulosic ethanol production process via co-culturing of artificial
cellulosomal Bacillus and kefir yeast," Appl. Energ., vol. 100, pp. 27-32,
2012.
[19] C. C. Lin, T. T. Liu, S. C. Kan, C. Z. Zang, et al., "Production of
D-hydantoinase via surface display and self-cleavage system," J. Biosci.
Bioeng., vol. 116, pp. 562-566, 2013.
[20] G. L. Miller, "Use of Dinitrosalicylic Acid Reagent for Determination of
Reducing Sugar," Anal. Chem., vol. 31 pp. 426 - 428, 1959.
[21] Y. M. Ko, C. I. Chen, C. C. Lin, S. C. Kan, et al., "Enhanced
D-hydantoinase activity performance via immobilized cobalt ion affinity
membrane and its kinetic study," Biochem. Eng. J., vol. 79, pp. 200-205,
2013.
[22] D. H. Northcote, K. J. Goulding, "The chemical composition and
structure of the cell wall of Chlorella pyrenoidosa," Biochem. J., vol. 70,
pp. 391–397, 1958.
[1] W. Han, W. Clarke, S. Pratt, "Composting of waste algae: A review,"
Waste Manage., vol. 34, pp. 1148-1155, 2014.
[2] C. C. Lin, C. H. Wei, C. I. Chen, C. J. Shieh, Y. C. Liu, "Characteristics of
the photosynthesis microbial fuel cell with a Spirulina platensis biofilm,"
Bioresource Technol., vol. 135, pp. 640-643, 2013.
[3] C. C. Fu, T. C. Hung, J. Y. Chen, C. H. Su, W. T. Wu, "Hydrolysis of
microalgae cell walls for production of reducing sugar and lipid
extraction," Bioresource Technol., vol. 101, pp. 8750-8754, 2010.
[4] Y. Chisti, "Biodiesel from microalgae," Biotechnol. Adv., vol. 25, pp.
294-306, 2007.
[5] C. H. Hsieh, W. T. Wu, "Cultivation of microalgae for oil production with
a cultivation strategy of urea limitation," Bioresource Technol., vol. 100,
pp. 3921-3926, 2009.
[6] E. Sanchez, K. Ojeda, M. El-Halwagi, V. Kafarov, "Biodiesel from
microalgae oil production in two sequential esterification/
transesterification reactors: Pinch analysis of heat integration," Chem.
Eng. J., vol. 176, pp. 211-216, 2011.
[7] Y. M. Dai, K. T. Chen, C. C. Chen, "Study of the microwave lipid
extraction from microalgae for biodiesel production," Chem. Eng. J., vol.
250, pp. 267-273, 2014.
[8] I. Rawat, R. R. Kumar, T. Mutanda, F. Bux, Appl. Energ. pp. 444-467.
2013,
[9] H. G. Gerken, B. Donohoe, E. P. Knoshaug, "Enzymatic cell wall
degradation of Chlorella vulgaris and other microalgae for biofuels
production," Planta, vol. 237, pp. 239-253, 2013
[10] M. Morweiser, O. Kruse, B. Hankamer, C. Posten, "Developments and
perspectives of photobioreactors for biofuel production," Appl.
Microbiol. Biotechnol., vol. 87, pp. 1291-1301, 2010.
[11] M. L. Ghirardi, J. P. Zhang, J. W. Lee, T. Flynn, et al., "Microalgae: a
green source of renewable H-2," Trends Biotechnol., vol. 18, pp. 506-511,
2000.
[12] J. B. Holm-Nielsen, T. Al Seadi, P. Oleskowicz-Popiel, "The future of
anaerobic digestion and biogas utilization," Bioresource Technol., vol.
100, pp. 5478-5484, 2009 [13] A. Vergara-Fernandez, G. Vargas, N. Alarcon, A. Velasco, "Evaluation of
marine algae as a source of biogas in a two-stage anaerobic reactor
system," Biomass Bioenerg., vol. 32, pp. 338-344, 2008.
[14] C. Y. Chen, M. D. Bai, J. S. Chang, "Improving microalgal oil collecting
efficiency by pretreating the microalgal cell wall with destructive
bacteria," Biochem. Eng. J., vol. 81, pp. 170-176, 2013.
[15] M. Girfoglio, M. Rossi, R. Cannio, "Cellulose Degradation by Sulfolobus
solfataricus Requires a Cell-Anchored Endo-beta-1-4-Glucanase," J.
Bacteriol., vol. 194, pp. 5091-5100, 2012.
[16] M. K. Bhat, "Cellulases and related enzymes in biotechnology,"
Biotechnol. Adv., vol. 18, pp. 355-383, 2000.
[17] W. H. Schwarz, "The cellulosome and cellulose degradation by anaerobic
bacteria," Appl. Microbiol. Biotechnol., vol. 56, pp. 634-649, 2001.
[18] C. Y. Ho, J. J. Chang, S. C. Lee, T. Y. Chin, et al., "Development of
cellulosic ethanol production process via co-culturing of artificial
cellulosomal Bacillus and kefir yeast," Appl. Energ., vol. 100, pp. 27-32,
2012.
[19] C. C. Lin, T. T. Liu, S. C. Kan, C. Z. Zang, et al., "Production of
D-hydantoinase via surface display and self-cleavage system," J. Biosci.
Bioeng., vol. 116, pp. 562-566, 2013.
[20] G. L. Miller, "Use of Dinitrosalicylic Acid Reagent for Determination of
Reducing Sugar," Anal. Chem., vol. 31 pp. 426 - 428, 1959.
[21] Y. M. Ko, C. I. Chen, C. C. Lin, S. C. Kan, et al., "Enhanced
D-hydantoinase activity performance via immobilized cobalt ion affinity
membrane and its kinetic study," Biochem. Eng. J., vol. 79, pp. 200-205,
2013.
[22] D. H. Northcote, K. J. Goulding, "The chemical composition and
structure of the cell wall of Chlorella pyrenoidosa," Biochem. J., vol. 70,
pp. 391–397, 1958.
@article{"International Journal of Biological, Life and Agricultural Sciences:70490", author = "C.-C. Lin and S.-C. Kan and C.-W. Yeh and C.-I Chen and C.-J. Shieh and Y.-C. Liu", title = "Kinetics Study for the Recombinant Cellulosome to the Degradation of Chlorella Cell Residuals", abstract = "In this study, lipid-deprived residuals of microalgae
were hydrolyzed for the production of reducing sugars by using the
recombinant Bacillus cellulosome, carrying eight genes from the
Clostridium thermocellum ATCC27405. The obtained cellulosome
was found to exist mostly in the broth supernatant with a cellulosome
activity of 2.4 U/mL. Furthermore, the Michaelis-Menten constant
(Km) and Vmax of cellulosome were found to be 14.832 g/L and 3.522
U/mL. The activation energy of the cellulosome to hydrolyze
microalgae LDRs was calculated as 32.804 kJ/mol.", keywords = "Lipid-deprived residuals of microalgae, cellulosome,
cellulose, reducing sugars, kinetics.", volume = "9", number = "7", pages = "777-4", }
