Extractive Fermentation of Ethanol Using Vacuum Fractionation Technique
A vacuum fractionation technique was introduced to remove ethanol from fermentation broth. The effect of initial glucose and ethanol concentrations were investigated for specific productivity. The inhibitory ethanol concentration was observed at 100 g/L. In order to increase the fermentation performance, the ethanol product was removed as soon as it is produced. The broth was boiled at 35oC by reducing the pressure to 65 mBar. The ethanol/water vapor was fractionated for up to 90 wt% before leaving the column. Ethanol concentration in the broth was kept lower than 25 g/L, thus minimized the product inhibition effect to the yeast cells. For batch extractive fermentation, a high substrate utilization rate was obtained at 26.6 g/L.h and most of glucose was consumed within 21 h. For repeated-batch extractive fermentation, addition of glucose was carried out up to 9 times and ethanol was produced more than 8-fold higher than batch fermentation.
[1] G.R. Cysewski, and C.R. Wilke, "Rapid ethanol fermentation using vacuum and cell recycle,” Biotechnol. Bioeng., Vol. 19, pp. 1125-1143, 1977.
[2] S.K.C. Lin, C. Du, A. Koutinas, R. Wang, and C. Webb, "Substrate and product inhibition kinetics in succinic acid production by Actinobacillus succinogenes,” Biochem. Eng. J., Vol. 41, pp. 128-135, 2008.
[3] G.J. Lye, and J.M. Woodley, "Application of in situ product removal techniques to biocatalytic processes,” Trend Biotechnol., Vol. 17, pp. 395-402, 1999.
[4] C. Chen, X. Tang, Z. Xiao, Y. Zhou, Y. Jiang, and S. Fu, "Ethanol fermentation kinetics in a continuous and closed-circulating system with a pervaporation membrane bioreactor,” Bioresource Technol., Vol. 114, pp. 707-710, 2012.
[5] D.J. O'Brien, G. Senske, M.J. Kurantz, and J.C. Jr. Craig, "Ethanol recovery from corn fiber hydrolysate fermentations by pervaporation,” Bioresource Technol., Vol. 92, pp.15-19, 2004.
[6] A. Thongsukmak, and K.K. Sirkar, "Extractive pervaporation to separate ethanol from its dilute aqueous solutions characteristic of ethanol-producing fermentation processes,” J. Membr. Sci., Vol. 329, pp. 119-129, 2009.
[7] M. Gryta, A.W. Morawski, and M. Tomaszewska, "Ethanol production in membrane distillation bioreactor,” Catal. Today., Vol. 56, pp. 159-165, 2000.
[8] G. Lewandowicz, W. Bialas, B. Marczewski, and D. Szymanowska, "Application of membrane distillation for ethanol recovery during fuel ethanol production,” J. Membr. Sci., Vol. 375, pp. 212-219, 2011.
[9] F. Tayler, M.A. Marquez, D.B. Johnston, N.M. Goldberg, and K.B. Hicks, "Continuous high-solids corn liquefaction and fermentation with stripping of ethanol,” Bioresource Technol., Vol. 101, pp. 4403-4408, 2010.
[10] R.D. Offeman, S.K. Stephenson, D. Franqui, J.L. Cline, G.H. Robertson, and W.J. Orts, "Extraction of ethanol with higher alcohol solvents and their toxicity to yeast,” Sep. Purif. Technol., Vol. 63, pp. 444-451, 2008.
[11] G.R. Cysewski, and C.R. Wilke, "Rapid ethanol fermentation using vacuum and cell recycle,” Biotechnol. Bioeng., Vol. 19, pp. 1125-1143, 1977.
[12] T.K. Ghose, P.K. Roychoudhury, and P. Ghose, "Simultaneous saccharification and fermentation (SSF) of ligocellulosics to ethanol under vacuum cycling and step feeding,” Biotechnol. Bioeng., Vol. 26, pp. 377-381, 1984.
[13] J.H. Lee, J.C. Woodard, R.J. Pagan, and P.L. Rogers, "Vacuum fermentation for ethanol production using strains of Zymomonas mobilis,” Biotechnol. Lett., Vol. 3, pp. 177-182, 1981.
[14] V.D. Nguyen, J. Auresenia, H. Kosuge, R.R. Tan, and Y. Brondial, "Vacuum fermentation integrated with separation process for ethanol production,” Biochem. Eng. J., Vol. 55, pp. 208-214, 2011.
[15] S. Chovau, G. Gaykawad, A.J.J. Straathof, and B. Van der Bruggen, "Influence of fermentation by-products on the purification of ethanol from water using pervaporation,” Bioresource Technol., Vol. 102, pp. 1669-1674, 2011.
[1] G.R. Cysewski, and C.R. Wilke, "Rapid ethanol fermentation using vacuum and cell recycle,” Biotechnol. Bioeng., Vol. 19, pp. 1125-1143, 1977.
[2] S.K.C. Lin, C. Du, A. Koutinas, R. Wang, and C. Webb, "Substrate and product inhibition kinetics in succinic acid production by Actinobacillus succinogenes,” Biochem. Eng. J., Vol. 41, pp. 128-135, 2008.
[3] G.J. Lye, and J.M. Woodley, "Application of in situ product removal techniques to biocatalytic processes,” Trend Biotechnol., Vol. 17, pp. 395-402, 1999.
[4] C. Chen, X. Tang, Z. Xiao, Y. Zhou, Y. Jiang, and S. Fu, "Ethanol fermentation kinetics in a continuous and closed-circulating system with a pervaporation membrane bioreactor,” Bioresource Technol., Vol. 114, pp. 707-710, 2012.
[5] D.J. O'Brien, G. Senske, M.J. Kurantz, and J.C. Jr. Craig, "Ethanol recovery from corn fiber hydrolysate fermentations by pervaporation,” Bioresource Technol., Vol. 92, pp.15-19, 2004.
[6] A. Thongsukmak, and K.K. Sirkar, "Extractive pervaporation to separate ethanol from its dilute aqueous solutions characteristic of ethanol-producing fermentation processes,” J. Membr. Sci., Vol. 329, pp. 119-129, 2009.
[7] M. Gryta, A.W. Morawski, and M. Tomaszewska, "Ethanol production in membrane distillation bioreactor,” Catal. Today., Vol. 56, pp. 159-165, 2000.
[8] G. Lewandowicz, W. Bialas, B. Marczewski, and D. Szymanowska, "Application of membrane distillation for ethanol recovery during fuel ethanol production,” J. Membr. Sci., Vol. 375, pp. 212-219, 2011.
[9] F. Tayler, M.A. Marquez, D.B. Johnston, N.M. Goldberg, and K.B. Hicks, "Continuous high-solids corn liquefaction and fermentation with stripping of ethanol,” Bioresource Technol., Vol. 101, pp. 4403-4408, 2010.
[10] R.D. Offeman, S.K. Stephenson, D. Franqui, J.L. Cline, G.H. Robertson, and W.J. Orts, "Extraction of ethanol with higher alcohol solvents and their toxicity to yeast,” Sep. Purif. Technol., Vol. 63, pp. 444-451, 2008.
[11] G.R. Cysewski, and C.R. Wilke, "Rapid ethanol fermentation using vacuum and cell recycle,” Biotechnol. Bioeng., Vol. 19, pp. 1125-1143, 1977.
[12] T.K. Ghose, P.K. Roychoudhury, and P. Ghose, "Simultaneous saccharification and fermentation (SSF) of ligocellulosics to ethanol under vacuum cycling and step feeding,” Biotechnol. Bioeng., Vol. 26, pp. 377-381, 1984.
[13] J.H. Lee, J.C. Woodard, R.J. Pagan, and P.L. Rogers, "Vacuum fermentation for ethanol production using strains of Zymomonas mobilis,” Biotechnol. Lett., Vol. 3, pp. 177-182, 1981.
[14] V.D. Nguyen, J. Auresenia, H. Kosuge, R.R. Tan, and Y. Brondial, "Vacuum fermentation integrated with separation process for ethanol production,” Biochem. Eng. J., Vol. 55, pp. 208-214, 2011.
[15] S. Chovau, G. Gaykawad, A.J.J. Straathof, and B. Van der Bruggen, "Influence of fermentation by-products on the purification of ethanol from water using pervaporation,” Bioresource Technol., Vol. 102, pp. 1669-1674, 2011.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:67971", author = "Weeraya Samnuknit and Apichat Boontawan", title = "Extractive Fermentation of Ethanol Using Vacuum Fractionation Technique", abstract = "A vacuum fractionation technique was introduced to remove ethanol from fermentation broth. The effect of initial glucose and ethanol concentrations were investigated for specific productivity. The inhibitory ethanol concentration was observed at 100 g/L. In order to increase the fermentation performance, the ethanol product was removed as soon as it is produced. The broth was boiled at 35oC by reducing the pressure to 65 mBar. The ethanol/water vapor was fractionated for up to 90 wt% before leaving the column. Ethanol concentration in the broth was kept lower than 25 g/L, thus minimized the product inhibition effect to the yeast cells. For batch extractive fermentation, a high substrate utilization rate was obtained at 26.6 g/L.h and most of glucose was consumed within 21 h. For repeated-batch extractive fermentation, addition of glucose was carried out up to 9 times and ethanol was produced more than 8-fold higher than batch fermentation.
", keywords = "Ethanol, Extractive fermentation, Product inhibition, Vacuum fractionation.", volume = "8", number = "9", pages = "1008-6", }
