Evaluation of Chlorophyll Content and Chlorophyll Fluorescence Parameters and Relationships between Chlorophyll a, b and Chlorophyll Content Index under Water Stress in Olea europaea cv. Dezful
This study was conducted to determine effect of water stress on chlorophyll content and chlorophyll fluorescence parameter in young `Dezful- olive trees. Three irrigation regimes (40% ETcrop, 65% ETcrop and 100% ETcrop) were used. After irrigation treatments were applied, some of biochemical parameters including chlorophyll a, b, total chlorophyll, chlorophyll fluorescence and also chlorophyll content index (C.C.I) were measured. Results of Analysis of variance showed that irrigation treatments had significant effect on chlorophylla, total chlorophyll (chl a+b), C.C.I and Fv/Fm ratio. The amount of decreased chlorophyll a and total chlorophyll in plants were received 40% ETcrop were 51.55% and 46.86%, respectively, compared with 100% ETcrop.
[1] S. Falk, D. P. Maxwell, D. E. Laudenbach, N. P.A. Huner, and N. R.
Baker, "In Advances in Photosynthesis, V.5, Photosynthesis and the
Environment". Kluwer Academic Publishers, Dordrecht Boston London.
1996, pp. 367-385.
[2] J.L. Araus, T. Amaro, J. Voltas, H. Nakkoul, and M.M Nachit, M M,
"Chlorophyll fluorescence as a selection criterion for grain yield in
durum wheat under Mediterranean conditions". Field Crops Research,
1998, Vol. 55, pp.209-223.
[3] P. Guo, and R. Li, Effects of high nocturnal temperature on
photosynthetic organization in rice leaves". Acta Botanica Sinica, 2000,
Vol. 42, pp. 13-18.
[4] Y. Fracheboud, C. Jompuk, J. M. Ribaut, P. Stamp, and J. Leipner,
"Genetic analysis of cold-tolerance of photosynthesis in maize". Plant Molecular Biology, 2004. Vol. 56, pp.241-253.
[5] K. Gregoriou, K. Pontikis, and S. Vemmos, "Effects of reduced
irradiance on leaf morphology, photosynthetic capacity, and fruit yield in
olive (Olea europaea L.)". Photosynthetica, 2007, Vol.45, No.2,
pp.172-181.
[6] F. Loreto, M. Centritto, and K. Chartzoulakis, "Photosynthetic
limitations in olive cultivars with different sensitivity to salt stress". Plant Cell Environ, 2003, Vol. 26, pp. 595-60 .
[7] J .C. Melgar, L. Guidi, D. Remorini, G. Agati, E. Degli-innocenti, S.
Castelli, M. C. Baratto, C. Faraloni, and M. Tattini, "Antioxidant
defences and oxidative-damage in salt-treated olive plants under
contrasting sunlight irradiance". Tree Physiol., 2009 29: 1187-1198.
[8] J. D. Hiscox, and G.F. Israelstam, " A method for the extraction of
chlorophyll from leaf tissue without maceration". Canadian Journal of
Botany, 1979, Vol. 57, pp.1332-1334.
[9] D.I, Arnon, "Copper enzymes in isolated chloroplasts.
Polyphenoloxidase in Beta vulgaris". Plant Physiology, 1949, Vol. 24, pp.1-15.
[10] G. H. Krause, and E. Weis, "Chlorophyll fluorescence and
photosynthesis: the basics". Annu Rev Plant Physiol Plant Mol Biol.,
1991. Vol. 42, pp. 313-349.
[11] F. Anjum, M. Yaseen, E. Rasul, A. Wahid, and S. Anjum, "Water stress
in barley (Hordeum vulgare L.). II. Effect on chemical composition and
chlorophyll contents". Pakistan J Agric Sci., 2003. Vol.40, pp. 45-49.
[12] M. Farooq, A. Wahid, N. Kobayashi, D. Fujita, and S. M. A. Basra,
ÔÇÿPlant drought stress: effects, mechanisms and management". Agron
Sustain Dev., 2009, Vol. 29, pp. 185-212
[13] A. Massacci,S. M. Nabiev, L. Pietrosanti, S. K. Nematov, T.N.
Chernikova, K. Thor, and J. Leipner, "Response of the photosynthetic
apparatus of cotton (Gossypium hirsutum) to the onset of drought stress
under field conditions studied by gas-exchange analysis and chlorophyll
fluorescence imaging". Plant Physiol Biochem., 2008, Vol. 46, pp.189-195
[14] S. P. Kiani, P. Maury, A. Sarrafi, and P. Grieu, “QTL analysis of
chlorophyll fluorescence parameters in sunflower (Helianthus annuus
L.) under well-watered and water-stressed conditions”. Plant Sci., 2008,
Vol. 175, pp. 565–573
[15] M. Guerfel, O. Baccouri, D. Boujnah, W. Chaı¨bi, and M. Zarrouk,
“Impacts of water stress on gas exchange, water relations, chlorophyll
content and leaf structure in the two main Tunisian olives (Olea
europaea L.) cultivars”. Scientia Horticulturae, 2009a, Vol.119, pp.
257–263.
[16] I. Arji, and K. Arzani, “Effect of water stress on some biochemical
changes in leaf of five olive (Olea europaea L.) cultivars”. Acta
Horticulturae, 2008, Vol. 791, pp. 523-526.
[17] K. Arzani, and N. Yazdani, “The influence of drought stress and
paclobutrazol on quantitative changes of proteins in olive (Olea
europaea L.) cultivars `Bladi' and `Mission'”. Acta Horticulturae , 2008,
Vol.791, pp.527-530.
[18] I. Arji, K. Arzani, H. Ebrahimzadeh, and R. Asghari, “Effect of Drought
stress on Physiological, morphological and biochemical characteristics
of some olive cultivars”. Thesis submitted in partial fulfillment of the
requirement for the degree of doctor of philosophy in Horticulture
Science, Agriculture Engineering. Tarbiat Modares University, Tehran.
Iran. 2003.
[19] M. Guerfel, Y. Ouni, D. Boujnah, and M. Zarrouk, “Photosynthesis
parameters and activities of enzymes of oxidative stress in two young ‘Chemlali’ and ‘Chetoui’ olive trees under water deficit”.
Photosynthetica, 2009b, Vol. 47, No.3, pp.340-346.
[20] B. Demming-Adams , W. W. Adams, D. H. Barker, B. A. Logan, D. R.
Bowling, and A. S. Verhoven, “Using Chlorophyll fluorescence to
assess the fraction of absorbed light allocated to thermal dissipation of
excess excitation”. Physiol. Plant, 1993, Vol. 98 pp. 253–264.
[21] E. A. Bacelar, “Ecophysiological responses of olive (Olea europaea L.)
to restricted water availability: Limitations, damages and drought
resistance mechanisms”. Universidade De Trás-Os-Montes E Alto Douro
Vila Real, 2006.
[22] C. Faraloni, I. Cutino, R. Petruccelli, A. R. Leva, S. Lazzeri, and G.
Torzillo,“Chlorophyll fluorescence technique as a rapid tool for in
vitro screening of olive cultivars” (Olea europaea L.) tolerant to drought
stress”. Environmental and Experimental Botany, 2011, Vol.73, pp.49-
56.
[23] T. M. Cate, and T. D. Perkins,” Chlorophyll content monitoring in sugar
maple (Acer saccharum)”. Tree Physiol., 2003, Vol. 23 No.15, pp.1077–
1079.
[24] B. L. Ma, and L. M. Dwyer, “Determination of nitrogen status in maize
senescing leaves”. J. Plant Nutr., 1997, Vol. 20, pp. 1–8.
[25] L. M. Dwyer, A. M. Anderson, B. L. Ma, D. W. Stewart, M. Tollenaar
and E. Gregorich, “Quantifying the nonlinearity in chlorophyll meter
response to corn leaf nitrogen concentration”. Can J Plant Sci., 1994,
Vol. 75, pp.179–182.
[26] D. W. Reeves, P.L Mask, C. W. Wood, and D. P. Delaney,
“Determination of wheat nitrogen status with a hand-held chlorophyll
meter: influence of management practices”. J Plant Nutr., 1993, Vol.16,
pp.781–796.
[27] R.J. Campbell, K. N. Mobley, R. P. Marini, and D. G. Pfeiffer “
Growing conditions alter the relationship between SPAD-501 values and
apple leaf chlorophyll”. Hortscience, 1990, Vol.25, pp.330–331.
[28] M. Ghasemi, K. Arzani, A. Yadollahi, S. Ghasemi, and S. Sarikhani
Khorrami, “Estimate of Leaf Chlorophyll and Nitrogen Content in Asian
Pear (Pyrus serotina Rehd.) by CCM-200”. Not Sci Biol.,2011, Vol.3,
No.1. pp.:91-94.
[29] D. Bussis, F. Kauder, and D. Heineke, “ Acclimation of potato plants to
polyethylene glycol-induced water deficit. I. Photosynthesis and
metabolism”. J Exp Bot., 1998, Vol. 49, pp.1349–1360.
[30] N. Smirnoff, “The role of active oxygen in the response of plants to
water deficit and desiccation”. New Phytol., 1993, Vol.125, pp.27–58.
[31] C. H. Foyer, and G. “Noctor, Oxygen processing in photosynthesis:
regulation and signaling”. New Phytol., 2000, Vol. 146, pp. 359-388.
[32] M. E. Younis, O. A. El-Shahaby, S. A. Abo-Hamed, and A. H.
Ibrahim,“Effects of water stress on growth, pigments and 14 CO2
assimilation in three sorghum cultivars”. Agron Crop Sci., 2000, Vol.
185, No. 2, pp: 73–82.
[33] J.F. Moran, M. Becan, I. Iturbe-Ormaetyl, S. Frechilla, R. V. Klucas, and
P. Aparicio-Tejo, “Drought induces oxidative stresses in pea plants”.
Planta, 1994, Vol.194, pp.346– 352.
[1] S. Falk, D. P. Maxwell, D. E. Laudenbach, N. P.A. Huner, and N. R.
Baker, "In Advances in Photosynthesis, V.5, Photosynthesis and the
Environment". Kluwer Academic Publishers, Dordrecht Boston London.
1996, pp. 367-385.
[2] J.L. Araus, T. Amaro, J. Voltas, H. Nakkoul, and M.M Nachit, M M,
"Chlorophyll fluorescence as a selection criterion for grain yield in
durum wheat under Mediterranean conditions". Field Crops Research,
1998, Vol. 55, pp.209-223.
[3] P. Guo, and R. Li, Effects of high nocturnal temperature on
photosynthetic organization in rice leaves". Acta Botanica Sinica, 2000,
Vol. 42, pp. 13-18.
[4] Y. Fracheboud, C. Jompuk, J. M. Ribaut, P. Stamp, and J. Leipner,
"Genetic analysis of cold-tolerance of photosynthesis in maize". Plant Molecular Biology, 2004. Vol. 56, pp.241-253.
[5] K. Gregoriou, K. Pontikis, and S. Vemmos, "Effects of reduced
irradiance on leaf morphology, photosynthetic capacity, and fruit yield in
olive (Olea europaea L.)". Photosynthetica, 2007, Vol.45, No.2,
pp.172-181.
[6] F. Loreto, M. Centritto, and K. Chartzoulakis, "Photosynthetic
limitations in olive cultivars with different sensitivity to salt stress". Plant Cell Environ, 2003, Vol. 26, pp. 595-60 .
[7] J .C. Melgar, L. Guidi, D. Remorini, G. Agati, E. Degli-innocenti, S.
Castelli, M. C. Baratto, C. Faraloni, and M. Tattini, "Antioxidant
defences and oxidative-damage in salt-treated olive plants under
contrasting sunlight irradiance". Tree Physiol., 2009 29: 1187-1198.
[8] J. D. Hiscox, and G.F. Israelstam, " A method for the extraction of
chlorophyll from leaf tissue without maceration". Canadian Journal of
Botany, 1979, Vol. 57, pp.1332-1334.
[9] D.I, Arnon, "Copper enzymes in isolated chloroplasts.
Polyphenoloxidase in Beta vulgaris". Plant Physiology, 1949, Vol. 24, pp.1-15.
[10] G. H. Krause, and E. Weis, "Chlorophyll fluorescence and
photosynthesis: the basics". Annu Rev Plant Physiol Plant Mol Biol.,
1991. Vol. 42, pp. 313-349.
[11] F. Anjum, M. Yaseen, E. Rasul, A. Wahid, and S. Anjum, "Water stress
in barley (Hordeum vulgare L.). II. Effect on chemical composition and
chlorophyll contents". Pakistan J Agric Sci., 2003. Vol.40, pp. 45-49.
[12] M. Farooq, A. Wahid, N. Kobayashi, D. Fujita, and S. M. A. Basra,
ÔÇÿPlant drought stress: effects, mechanisms and management". Agron
Sustain Dev., 2009, Vol. 29, pp. 185-212
[13] A. Massacci,S. M. Nabiev, L. Pietrosanti, S. K. Nematov, T.N.
Chernikova, K. Thor, and J. Leipner, "Response of the photosynthetic
apparatus of cotton (Gossypium hirsutum) to the onset of drought stress
under field conditions studied by gas-exchange analysis and chlorophyll
fluorescence imaging". Plant Physiol Biochem., 2008, Vol. 46, pp.189-195
[14] S. P. Kiani, P. Maury, A. Sarrafi, and P. Grieu, “QTL analysis of
chlorophyll fluorescence parameters in sunflower (Helianthus annuus
L.) under well-watered and water-stressed conditions”. Plant Sci., 2008,
Vol. 175, pp. 565–573
[15] M. Guerfel, O. Baccouri, D. Boujnah, W. Chaı¨bi, and M. Zarrouk,
“Impacts of water stress on gas exchange, water relations, chlorophyll
content and leaf structure in the two main Tunisian olives (Olea
europaea L.) cultivars”. Scientia Horticulturae, 2009a, Vol.119, pp.
257–263.
[16] I. Arji, and K. Arzani, “Effect of water stress on some biochemical
changes in leaf of five olive (Olea europaea L.) cultivars”. Acta
Horticulturae, 2008, Vol. 791, pp. 523-526.
[17] K. Arzani, and N. Yazdani, “The influence of drought stress and
paclobutrazol on quantitative changes of proteins in olive (Olea
europaea L.) cultivars `Bladi' and `Mission'”. Acta Horticulturae , 2008,
Vol.791, pp.527-530.
[18] I. Arji, K. Arzani, H. Ebrahimzadeh, and R. Asghari, “Effect of Drought
stress on Physiological, morphological and biochemical characteristics
of some olive cultivars”. Thesis submitted in partial fulfillment of the
requirement for the degree of doctor of philosophy in Horticulture
Science, Agriculture Engineering. Tarbiat Modares University, Tehran.
Iran. 2003.
[19] M. Guerfel, Y. Ouni, D. Boujnah, and M. Zarrouk, “Photosynthesis
parameters and activities of enzymes of oxidative stress in two young ‘Chemlali’ and ‘Chetoui’ olive trees under water deficit”.
Photosynthetica, 2009b, Vol. 47, No.3, pp.340-346.
[20] B. Demming-Adams , W. W. Adams, D. H. Barker, B. A. Logan, D. R.
Bowling, and A. S. Verhoven, “Using Chlorophyll fluorescence to
assess the fraction of absorbed light allocated to thermal dissipation of
excess excitation”. Physiol. Plant, 1993, Vol. 98 pp. 253–264.
[21] E. A. Bacelar, “Ecophysiological responses of olive (Olea europaea L.)
to restricted water availability: Limitations, damages and drought
resistance mechanisms”. Universidade De Trás-Os-Montes E Alto Douro
Vila Real, 2006.
[22] C. Faraloni, I. Cutino, R. Petruccelli, A. R. Leva, S. Lazzeri, and G.
Torzillo,“Chlorophyll fluorescence technique as a rapid tool for in
vitro screening of olive cultivars” (Olea europaea L.) tolerant to drought
stress”. Environmental and Experimental Botany, 2011, Vol.73, pp.49-
56.
[23] T. M. Cate, and T. D. Perkins,” Chlorophyll content monitoring in sugar
maple (Acer saccharum)”. Tree Physiol., 2003, Vol. 23 No.15, pp.1077–
1079.
[24] B. L. Ma, and L. M. Dwyer, “Determination of nitrogen status in maize
senescing leaves”. J. Plant Nutr., 1997, Vol. 20, pp. 1–8.
[25] L. M. Dwyer, A. M. Anderson, B. L. Ma, D. W. Stewart, M. Tollenaar
and E. Gregorich, “Quantifying the nonlinearity in chlorophyll meter
response to corn leaf nitrogen concentration”. Can J Plant Sci., 1994,
Vol. 75, pp.179–182.
[26] D. W. Reeves, P.L Mask, C. W. Wood, and D. P. Delaney,
“Determination of wheat nitrogen status with a hand-held chlorophyll
meter: influence of management practices”. J Plant Nutr., 1993, Vol.16,
pp.781–796.
[27] R.J. Campbell, K. N. Mobley, R. P. Marini, and D. G. Pfeiffer “
Growing conditions alter the relationship between SPAD-501 values and
apple leaf chlorophyll”. Hortscience, 1990, Vol.25, pp.330–331.
[28] M. Ghasemi, K. Arzani, A. Yadollahi, S. Ghasemi, and S. Sarikhani
Khorrami, “Estimate of Leaf Chlorophyll and Nitrogen Content in Asian
Pear (Pyrus serotina Rehd.) by CCM-200”. Not Sci Biol.,2011, Vol.3,
No.1. pp.:91-94.
[29] D. Bussis, F. Kauder, and D. Heineke, “ Acclimation of potato plants to
polyethylene glycol-induced water deficit. I. Photosynthesis and
metabolism”. J Exp Bot., 1998, Vol. 49, pp.1349–1360.
[30] N. Smirnoff, “The role of active oxygen in the response of plants to
water deficit and desiccation”. New Phytol., 1993, Vol.125, pp.27–58.
[31] C. H. Foyer, and G. “Noctor, Oxygen processing in photosynthesis:
regulation and signaling”. New Phytol., 2000, Vol. 146, pp. 359-388.
[32] M. E. Younis, O. A. El-Shahaby, S. A. Abo-Hamed, and A. H.
Ibrahim,“Effects of water stress on growth, pigments and 14 CO2
assimilation in three sorghum cultivars”. Agron Crop Sci., 2000, Vol.
185, No. 2, pp: 73–82.
[33] J.F. Moran, M. Becan, I. Iturbe-Ormaetyl, S. Frechilla, R. V. Klucas, and
P. Aparicio-Tejo, “Drought induces oxidative stresses in pea plants”.
Planta, 1994, Vol.194, pp.346– 352.
@article{"International Journal of Biological, Life and Agricultural Sciences:63030", author = "E. Khaleghi and K. Arzani and N. Moallemi and M. Barzegar", title = "Evaluation of Chlorophyll Content and Chlorophyll Fluorescence Parameters and Relationships between Chlorophyll a, b and Chlorophyll Content Index under Water Stress in Olea europaea cv. Dezful", abstract = "This study was conducted to determine effect of water stress on chlorophyll content and chlorophyll fluorescence parameter in young `Dezful- olive trees. Three irrigation regimes (40% ETcrop, 65% ETcrop and 100% ETcrop) were used. After irrigation treatments were applied, some of biochemical parameters including chlorophyll a, b, total chlorophyll, chlorophyll fluorescence and also chlorophyll content index (C.C.I) were measured. Results of Analysis of variance showed that irrigation treatments had significant effect on chlorophylla, total chlorophyll (chl a+b), C.C.I and Fv/Fm ratio. The amount of decreased chlorophyll a and total chlorophyll in plants were received 40% ETcrop were 51.55% and 46.86%, respectively, compared with 100% ETcrop.
", keywords = "Evatarnspiration (ETcrop), Chlorophyll Content, Chlorophyll Fluorescence, Water stress, Olive", volume = "6", number = "8", pages = "636-4", }
