Estimating Enzyme Kinetic Parameters from Apparent KMs and Vmaxs

The kinetic properties of enzymes are often reported using the apparent KM and Vmax appropriate to the standard Michaelis-Menten enzyme. However, this model is inappropriate to enzymes that have more than one substrate or where the rate expression does not apply for other reasons. Consequently, it is desirable to have a means of estimating the appropriate kinetic parameters from the apparent values of KM and Vmax reported for each substrate. We provide a means of estimating the range within which the parameters should lie and apply the method to data for glutamate dehydrogenase from the nematode parasite of sheep Teladorsagia circumcincta.

Authors:

• Simon Brown
• Noorzaid Muhamad
• David C Simcock

Keywords:

• enzyme kinetics
• glutamate dehydrogenase
• intervalanalysis
• parameter estimation.

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