Enhanced Efficiency for Propagation of Phalaenopsis cornu-cervi (Breda) Blume & Rchb. F. Using Trimmed Leaf Technique

The effects of thidiazuron (TDZ) and benzyladenine (BA) on protocorm-like bodies (PLBs) induction from leaf explants was investigated. It was found that TDZ was superior to BA. The highest percentage and number of PLBs per leaf explant at 30 and 5.3, respectively were obtained on ½ MS medium supplemented with 9µM TDZ. The regenerated plantlets were potted and acclimatized in the greenhouse. These plants grew well and developed into normal plants after 3 month of transplantation. The 100% survival of plantlets was achieved when planted on pots containing sphagnum moss. 

• Suphat Rittirat
• Sutha Klaocheed
• Kanchit Thammasiri

• Orchid
• PLBs
• sphagnum moss
• thidiazuron.

[1] P. Kośir, S. Škof, and Z. dan Luthar, "Direct shoot regeneration from nodes of Phalaenopsis orchids.” Acta Agric. Sloven, vol. 83(2), pp. 233-242, 2004.
[2] K. Tokuhara, and M. Mii, "Micropropagation of Pha¬laenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds.” Plant Cell Rep, vol. 13, pp. 7-11, 1993.
[3] T. Murashige, and F. Skoog, "A revised medium for rapid growth and bioassays with tobacco tissue cultures.” Physiol. Plant, vol. 15, pp. 473-497, 1962.
[4] S. Y. Park, E. C. Yeung, and D. Chakrabarty, "An efficient direct induction of Protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture.” Plant Cell Rep, vol. 21, pp. 46-51, 2002.
[5] N. R. Nayak, S. Patnaik, and S. P. Rath, "Direct shoot regeneration from foliar explants of an epiphytic orchid Acampe praemorsa (Roxb.) Blatter and McCann.” Plant Cell Rep, vol. 16, pp. 583-586, 1997.
[6] J. T. Chen, and W. C. Chang, "Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis.” Biologia Plantarum, vol. 50, pp. 169-173, 2006.
[7] C. Chang, and W. C. Chang, "Plant regeneration from callus culture of Cymbidium ensifolium var. misericors.” Plant Cell Rep, vol. 17, pp. 251-255, 1998.
[8] R. Ernst, "Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidacea).” Plant Cell Tissue Organ Cult, vol. 39, pp. 273-275, 1994.