Effect of Sperm Concentration and Length of Storage at 5 C on Motility of Goat Spermatozoa
The objective of the present study was to determine
the effect of different concentration of spermatozoa and length of
storage in 5 0C on sperm motility. Semen was collected using
artificial vagina from goat aged 2 to 2.5 years. Fresh goat semen
with sperm motility ≥ 70% was used as material. Semen was
divided into 4 treatments of concentration (40 x 10 6 / ml, 50 x
106/ml, 60x106/ml, 70x106/ml) with length of storage 0,12,24,36 h. in
5 0C. There were interactions (P <0.05) between concentration and
length of storage on individual motility of spermatozoa.
Concentration of spermatozoa and length of storage affect the
motility of individual (P <0.05). It was concluded that Sperm
motility will decrease with increasing concentration and length of
storage in 5 0C. Concentration of sperm 40x106/ ml and length of
storage 0 h in 50C showed the highest motility of spermatozoa
[1] O. J.E. Parks and J.K.Graham. 1992. Effects of cryopreservation
procedures on sperm membranes. Theriogenology;38:209-22.
[2] J. Gadea. F.G. Vasquez, C. Matas, J.C. Gardon, S. Canovas and D.
Gumbao. 2005. Cooling and freezing boar spermatozoa: suplementation
of the freezing media with reduce gluthatione preserves sperm function .
J. Andrology.6:3
[3] P.F. Watson. 2000. The causes of reduced fertility with cryopreserved
semen. Anim Reprod Sci 61:481-92.
[4] J. Dorado, A.M.Serrano and M.Hidalgo.2010. The effect of
cryopreservation on goat semen characteristics related to sperm
freezability. J. Reprod. Sci 121 : 115-123
[5] S. Kumar, J.D.Millar and P.F.Watson. 2003. The effect of cooling rate
on survival of cryopreserved bull,ram and boar spermatozoa : A
comparison of two controlled-rate cooling machines. Cryobiology 46 :
246-253.
[6] B.T.Zhao, D. Han , C.L. Xu , M.J. Luo , Z.L. Chang and J.H.Tan ,
2009. Protocol optimation for long term liquid storage of goat semen in
a chemically defined extender. J. Reproduction in Domestic Animals.
44:865-872
[7] A. Bergeron and P. Manjunath, 2006. New insight towards
understanding the mechanism of sperm protection by egg yolk and milk.
J. Molecular Reproduction and Development 73:1338-1344
[8] R. Ax., Dally. M., Didion. B., Lenz. R., Love. C., Varner. D., Hafez, and
Bellin. M.. 2000. Semen Evaluation. In Reproduction in Farm Animal.
Edited By Hafez, E. S. E., and B. Hafez 7th Edition. Lippincott Williams
& Wilkins. Philadelphia. USA: 365-369.
[9] S. Chatterjee, C. Gagnon. 2001. Production of reactive oxygen species
by spermatozoa undergoing cooling, freezing and thawing. Mol Reprod
Dev 59:451-458.
[10] S.A. Agarwal, Prabakaran, T.M. Said. 2005. Prevention of oxidative
stress injury to sperm. J. Andrology 26 : 654-660
[11] L.Janice, Bailey, N. Cormier. 2000. Semen cryopreservation in
domestic animals : A damaging and capacitating phenomenon. J.
Andrology Vol. 21. No.1
[12] M.N. Munsi, M.M.U. Buiyan, M.G.S. Alam. 2007. Effects of
Exogenous Glutathione on the Quality of Chilled Bull Semen.
Reproduction in Domestic Animal Vol 42:358-362.
[13] G. Kadirvel, S.Kumar and A. Kumaresan. 2009. Lipid
peroxidation,mitochondrial membrane potential and DNA integrity of
spermatozoa in relation to intracellular reactive oxygen spesies in liquid
and frozen-thawed buffalo semen. J. Animal Reproduction Science
114(1-3): 125-134
[14] E.M.E.Aboagla, T. Terada, 2004: Effects of the supplementation of
trehalose extender containing egg yolk with sodium dodecyl sulfate on
the freezability of goat spermatozoa. Theriogenology 62, 809-818.
[15] J.F.Fonseca, C.A.A.Torres,, V.V. Maffili, A.M. Borges, A.D.F. Santos,
M.T. Rodrigues and Oliveira. 2005. The hypoosmotic swelling test in
fresh goat spermatozoa. J. Anim. Reprod. 2:139-144
[1] O. J.E. Parks and J.K.Graham. 1992. Effects of cryopreservation
procedures on sperm membranes. Theriogenology;38:209-22.
[2] J. Gadea. F.G. Vasquez, C. Matas, J.C. Gardon, S. Canovas and D.
Gumbao. 2005. Cooling and freezing boar spermatozoa: suplementation
of the freezing media with reduce gluthatione preserves sperm function .
J. Andrology.6:3
[3] P.F. Watson. 2000. The causes of reduced fertility with cryopreserved
semen. Anim Reprod Sci 61:481-92.
[4] J. Dorado, A.M.Serrano and M.Hidalgo.2010. The effect of
cryopreservation on goat semen characteristics related to sperm
freezability. J. Reprod. Sci 121 : 115-123
[5] S. Kumar, J.D.Millar and P.F.Watson. 2003. The effect of cooling rate
on survival of cryopreserved bull,ram and boar spermatozoa : A
comparison of two controlled-rate cooling machines. Cryobiology 46 :
246-253.
[6] B.T.Zhao, D. Han , C.L. Xu , M.J. Luo , Z.L. Chang and J.H.Tan ,
2009. Protocol optimation for long term liquid storage of goat semen in
a chemically defined extender. J. Reproduction in Domestic Animals.
44:865-872
[7] A. Bergeron and P. Manjunath, 2006. New insight towards
understanding the mechanism of sperm protection by egg yolk and milk.
J. Molecular Reproduction and Development 73:1338-1344
[8] R. Ax., Dally. M., Didion. B., Lenz. R., Love. C., Varner. D., Hafez, and
Bellin. M.. 2000. Semen Evaluation. In Reproduction in Farm Animal.
Edited By Hafez, E. S. E., and B. Hafez 7th Edition. Lippincott Williams
& Wilkins. Philadelphia. USA: 365-369.
[9] S. Chatterjee, C. Gagnon. 2001. Production of reactive oxygen species
by spermatozoa undergoing cooling, freezing and thawing. Mol Reprod
Dev 59:451-458.
[10] S.A. Agarwal, Prabakaran, T.M. Said. 2005. Prevention of oxidative
stress injury to sperm. J. Andrology 26 : 654-660
[11] L.Janice, Bailey, N. Cormier. 2000. Semen cryopreservation in
domestic animals : A damaging and capacitating phenomenon. J.
Andrology Vol. 21. No.1
[12] M.N. Munsi, M.M.U. Buiyan, M.G.S. Alam. 2007. Effects of
Exogenous Glutathione on the Quality of Chilled Bull Semen.
Reproduction in Domestic Animal Vol 42:358-362.
[13] G. Kadirvel, S.Kumar and A. Kumaresan. 2009. Lipid
peroxidation,mitochondrial membrane potential and DNA integrity of
spermatozoa in relation to intracellular reactive oxygen spesies in liquid
and frozen-thawed buffalo semen. J. Animal Reproduction Science
114(1-3): 125-134
[14] E.M.E.Aboagla, T. Terada, 2004: Effects of the supplementation of
trehalose extender containing egg yolk with sodium dodecyl sulfate on
the freezability of goat spermatozoa. Theriogenology 62, 809-818.
[15] J.F.Fonseca, C.A.A.Torres,, V.V. Maffili, A.M. Borges, A.D.F. Santos,
M.T. Rodrigues and Oliveira. 2005. The hypoosmotic swelling test in
fresh goat spermatozoa. J. Anim. Reprod. 2:139-144
@article{"International Journal of Biological, Life and Agricultural Sciences:56491", author = "Sri Wahjuningsih and Hermanto and Nuryadi and Agus Budiarto and Panji Bhintoro", title = "Effect of Sperm Concentration and Length of Storage at 5 C on Motility of Goat Spermatozoa", abstract = "The objective of the present study was to determine
the effect of different concentration of spermatozoa and length of
storage in 5 0C on sperm motility. Semen was collected using
artificial vagina from goat aged 2 to 2.5 years. Fresh goat semen
with sperm motility ≥ 70% was used as material. Semen was
divided into 4 treatments of concentration (40 x 10 6 / ml, 50 x
106/ml, 60x106/ml, 70x106/ml) with length of storage 0,12,24,36 h. in
5 0C. There were interactions (P ", keywords = "Goat, Length of storage, Motility, Sperm Concentration", volume = "6", number = "6", pages = "353-3", }
