Effect of Cold Plasma-Surface Modification on Surface Wettability and Initial Cell Attachment
A thin coating of hexamethyldisiloxane and subsequent O2-plasma treatment was performed on mirror-polished titanium in order to regulate the wide range of wettability including 106 and almost 0 degrees of contact angles. The adsorption behavior of
fibronectin and albumin in both individual and competitive mode,
and initial attachment of fibroblasts and osteoblasts were investigated.
Individually, fibronectin adsorption showed a biphasic inclination, whereas albumin showed greater adsorption to hydrophobic surfaces.
In competitive mode, in solution containing both fibronectin and albumin, fibronectin showed greater adsorption on hydrophilic
surfaces, whereas Alb predominantly adsorbed on hydrophobic
surfaces. Initial attachment of both cells increased with increase in
surface wettability, in particular, on super-hydrophilic surface, which
correlated well with fibronectin adsorption in competitive mode.
These results suggest that a cold plasma-surface modification enabled
to regulate the surface wettability, and fibronectin adsorption may be
responsible for increasing cell adhesion on hydrophilic surfaces in a
body fluid
[1] S.H. Lee, D.C. Lee, "Preparation and characterization of thin films by plasma polymerization of hexamethyldisiloxane," Thin Solid Films, vol.325, pp.83-86, 1998.
[2] J. Schwarz, M. Schmidt, A. Ohl, "Synthesis of plasma-polymerized hexamethyldisiloxane (HMDSO) films by microwave discharge," Surf.
Coat. Technol., vol.98, pp.859-864, 1998.
[3] B. Janocha, D. Hegemann, C. Oehra, H. Brunner, F. Rupp, J.
Geis-Gerstorfer, "Adsorption of protein on plasma-polysiloxane layers
of different surface energies," Surf. Coat. Technol., vol.142-144, pp. 1051-1055, 2001.
[4] G.C.M. Steffens, L. Nothdurft, G. Buse, H. Thissen, H. Hocker, D. Klee,
"High density binding of proteins and peptides to poly(,-lactide) grafted
with polyacrylic acid," Biomater., vol. 23, pp. 3523-3531, 2002.
[5] I. Bisson, M. Kosinski, S. Ruault, B. Gupta, J. Hilborn, F. Wurm, P. Frey, "Acrylic acid grafting and collagen immobilization on
poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells," Biomater., vol. 23, pp. 3149-3158,2002.
[6] L. Jui-Chea, S.L. Cooper, "Surface characterization and ex vivo blood
compatibility study of plasma-modified small diameter tubing: Effect of
sulphur dioxide and hexamethyl-disiloxane plasmas," Biomater., vol. 16,
pp.1017-1023, 1995.
[7] R. Ganapathy, M. Sarmadi, F. Denes, "Immobilization of
alpha-chymotrypsin on oxygen RF-plasma functionalized PET and PP
surfaces," J. Biomater. Sci. Polym. Ed., vol. 9, pp. 389-404. 1998.
[8] Z. Wu, N. Xanthopoulos, F. Reymond, J.S. Rossier, H.H. Girault,
"Polymer microchips bonded by O2-plasma activation,"
Electrophoresis, vol. 23, pp.782-790, 2002.
[9] J.L. Dewez, A. Doren, Y.J. Schneider, P.G. Rouxhet, "Competitive
adsorption of proteins key of the relationship between substratum
surface properties and adhesion of epithelial cells," Biomater., vol.20,
pp.547-559, 1999.
[10] S.R. Sousa, M. Lamghari, P. Sampaio, P. Moradas-Ferreira, M.A.
Barbosa, "Osteoblast adhesion and morphology on TiO2 depends on the
competitive preadsorption of albumin and fibronectin," J. Biomed. Mater. Res., vol.84, pp.281-290, 2008.
[11] Y. Ozdemir, N. Hasirci, K. Serbetci, "Oxygen plasma modification of
polyurethane membranes," J. Mater. Sci. Mater. Med., vol.13, pp.1147-1151, 2002.
[12] T. Hayakawa, M. Yoshinari, K. Nemoto, "Characterization and
protein-adsorption behavior of deposited organic thin film onto titanium
by plasma polymerization with hexamethyldisiloxane," Biomater., vol.1,
pp.69-79, 2004.
[13] M. Yoshinari, T. Hayakawa, K. Matsuzaka, T. Inoue, Y. Oda, M.
Shimono, "Immobilization of fibronectin onto organic
hexamethyldisiloxane coating with plasma surface modification
-analysis of fibronectin adsorption using quartz crystal microbalance-dissipation technique-, " J. Oral. Tissue Engin. 27,
pp.29-36, 2006.
[14] F. Grinnell, M.K. Feld, "Fibronectin adsorption on hydrophilic and
hydrophobic surfaces detected by antibody binding and analyzed during
cell adhesion in serum-containing medium," J. Biol. Chem., vol.257,
pp.4888-4893, 1982.
[15] L. Hao, J. Lawrence, "Albumin and fibronectin protein adsorption on
CO2-laser-modified biograde stainless steel," Proc. Inst. Mech. Eng., vol. 220, pp.47-55, 2006.
[16] L. de Bartolo, S. Morelli, A. Piscioneri, L.C. Lopez, P. Favia, R. d'Agostino, E. Drioli, "Novel membranes and surface modification
able to activate specific cellular responses," Biomol. Eng., vol.24, pp.23-26, 2007.
[1] S.H. Lee, D.C. Lee, "Preparation and characterization of thin films by plasma polymerization of hexamethyldisiloxane," Thin Solid Films, vol.325, pp.83-86, 1998.
[2] J. Schwarz, M. Schmidt, A. Ohl, "Synthesis of plasma-polymerized hexamethyldisiloxane (HMDSO) films by microwave discharge," Surf.
Coat. Technol., vol.98, pp.859-864, 1998.
[3] B. Janocha, D. Hegemann, C. Oehra, H. Brunner, F. Rupp, J.
Geis-Gerstorfer, "Adsorption of protein on plasma-polysiloxane layers
of different surface energies," Surf. Coat. Technol., vol.142-144, pp. 1051-1055, 2001.
[4] G.C.M. Steffens, L. Nothdurft, G. Buse, H. Thissen, H. Hocker, D. Klee,
"High density binding of proteins and peptides to poly(,-lactide) grafted
with polyacrylic acid," Biomater., vol. 23, pp. 3523-3531, 2002.
[5] I. Bisson, M. Kosinski, S. Ruault, B. Gupta, J. Hilborn, F. Wurm, P. Frey, "Acrylic acid grafting and collagen immobilization on
poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells," Biomater., vol. 23, pp. 3149-3158,2002.
[6] L. Jui-Chea, S.L. Cooper, "Surface characterization and ex vivo blood
compatibility study of plasma-modified small diameter tubing: Effect of
sulphur dioxide and hexamethyl-disiloxane plasmas," Biomater., vol. 16,
pp.1017-1023, 1995.
[7] R. Ganapathy, M. Sarmadi, F. Denes, "Immobilization of
alpha-chymotrypsin on oxygen RF-plasma functionalized PET and PP
surfaces," J. Biomater. Sci. Polym. Ed., vol. 9, pp. 389-404. 1998.
[8] Z. Wu, N. Xanthopoulos, F. Reymond, J.S. Rossier, H.H. Girault,
"Polymer microchips bonded by O2-plasma activation,"
Electrophoresis, vol. 23, pp.782-790, 2002.
[9] J.L. Dewez, A. Doren, Y.J. Schneider, P.G. Rouxhet, "Competitive
adsorption of proteins key of the relationship between substratum
surface properties and adhesion of epithelial cells," Biomater., vol.20,
pp.547-559, 1999.
[10] S.R. Sousa, M. Lamghari, P. Sampaio, P. Moradas-Ferreira, M.A.
Barbosa, "Osteoblast adhesion and morphology on TiO2 depends on the
competitive preadsorption of albumin and fibronectin," J. Biomed. Mater. Res., vol.84, pp.281-290, 2008.
[11] Y. Ozdemir, N. Hasirci, K. Serbetci, "Oxygen plasma modification of
polyurethane membranes," J. Mater. Sci. Mater. Med., vol.13, pp.1147-1151, 2002.
[12] T. Hayakawa, M. Yoshinari, K. Nemoto, "Characterization and
protein-adsorption behavior of deposited organic thin film onto titanium
by plasma polymerization with hexamethyldisiloxane," Biomater., vol.1,
pp.69-79, 2004.
[13] M. Yoshinari, T. Hayakawa, K. Matsuzaka, T. Inoue, Y. Oda, M.
Shimono, "Immobilization of fibronectin onto organic
hexamethyldisiloxane coating with plasma surface modification
-analysis of fibronectin adsorption using quartz crystal microbalance-dissipation technique-, " J. Oral. Tissue Engin. 27,
pp.29-36, 2006.
[14] F. Grinnell, M.K. Feld, "Fibronectin adsorption on hydrophilic and
hydrophobic surfaces detected by antibody binding and analyzed during
cell adhesion in serum-containing medium," J. Biol. Chem., vol.257,
pp.4888-4893, 1982.
[15] L. Hao, J. Lawrence, "Albumin and fibronectin protein adsorption on
CO2-laser-modified biograde stainless steel," Proc. Inst. Mech. Eng., vol. 220, pp.47-55, 2006.
[16] L. de Bartolo, S. Morelli, A. Piscioneri, L.C. Lopez, P. Favia, R. d'Agostino, E. Drioli, "Novel membranes and surface modification
able to activate specific cellular responses," Biomol. Eng., vol.24, pp.23-26, 2007.
@article{"International Journal of Biological, Life and Agricultural Sciences:53599", author = "Masao Yoshinari and Jianhua Wei and Kenichi Matsuzaka and Takashi Inoue", title = "Effect of Cold Plasma-Surface Modification on Surface Wettability and Initial Cell Attachment", abstract = "A thin coating of hexamethyldisiloxane and subsequent O2-plasma treatment was performed on mirror-polished titanium in order to regulate the wide range of wettability including 106 and almost 0 degrees of contact angles. The adsorption behavior of
fibronectin and albumin in both individual and competitive mode,
and initial attachment of fibroblasts and osteoblasts were investigated.
Individually, fibronectin adsorption showed a biphasic inclination, whereas albumin showed greater adsorption to hydrophobic surfaces.
In competitive mode, in solution containing both fibronectin and albumin, fibronectin showed greater adsorption on hydrophilic
surfaces, whereas Alb predominantly adsorbed on hydrophobic
surfaces. Initial attachment of both cells increased with increase in
surface wettability, in particular, on super-hydrophilic surface, which
correlated well with fibronectin adsorption in competitive mode.
These results suggest that a cold plasma-surface modification enabled
to regulate the surface wettability, and fibronectin adsorption may be
responsible for increasing cell adhesion on hydrophilic surfaces in a
body fluid", keywords = "cold plasma-surface modification, wettability, protein adsorption, initial cell attachment.", volume = "3", number = "10", pages = "497-5", }
