Effect of Bio-Nitrogen as a Partial Alternative to Mineral-Nitrogen Fertiliser on Growth, Nitrate and Nitrite Contents, and Yield Quality in Brassica oleracea L.
Effects of bio-nitrogen fertilizer (bio-N), as a partial
alternative to mineral-nitrogen fertilizer (mineral-N), on growth,
yield and yield quality of broccoli plants were investigated. Bio-N
was applied at 1, 2 or 3 doses in combination with 65% of the
recommended dose of mineral-N (bio-N1, bio-N2 or bio-N3 +
⅔mineral-N). However, 100% of the recommended dose of mineral-
N was applied as a control. Significant positive influences of the bio-
N3 + ⅔mineral-N treatment were observed on growth traits, leaf
contents of nitrogen, phosphorus, potassium, nitrate and nitrite, and
yield quality when compared to the other two combined treatments.
In contrast, there were no significant differences in these parameters
between the bio-N3 + ⅔mineral-N and the control treatments, except
for leaf contents of nitrate and nitrite. They showed lower contents in
the bio-N3 + ⅔mineral-N treatment than the control. Therefore, we
recommend using bio-N as a partial alternative to mineral-N for
healthy nutrition.
<p>[1] A. Hartman, “Ecophysiological aspects of growth and nitrogen fixation
in Azospirillium species”. Plant Soil, vol. 110, 1988, pp. 225–238.
[2] N. P. Singh, R. S. Sachan, P. C. Pandey, and P. S. Bisht, “Effect of a
decade long fertilizer and manure application on soil fertility and
productivity of rice-wheat system in Molisols”. J. Indian Soc. Soil Sci.,
vol. 47, 1999, pp. 72–80.
[3] A. Bhatia, H. Pathak, and H. C. Joshi, “Use of sewage as a source of
plant nutrient: potentials and problems”. Fert. News, vol. 46, 2001, pp.
55–58.
[4] C. A. Palm, C. N. Gachengo, R. J. Delve, G. Cadisch, and K. E. Giller,
“Organic inputs for soil fertility management in tropical agro
ecosystems: application of an organic resource database”. Agric.
Ecosyst. Environ, vol. 83, 2001, pp. 27–42.
[5] E. C. Cocking, “Helping plants get more nitrogen from air”. European
Review, vol. 8, 2000, pp. 193–200.
[6] A. N. Ibrahim, and I. M. Abd El-Aziz, “Solubilization of rock phosphate
by streptomyces”. Agr. Talajton, vol. 26, 1977, pp. 424–434.
[7] M. Fayez, N. F. Eman, and H. E. Makbol, “The possible use of nitrogen
fixing Azospirillum as biofertilizer for wheat plants”. Egypt. J.
Microbiol., vol. 20, 1985, pp. 199–206.
[8] M. R. Abdel-Latif, A. A. El-Bana, and A. A. Galal, “Effect of
biofertilizers Microbein and Phosphorine on bacterial pod blight of guar
and black cumin damping off root rot and with diseases”. Proc. of the
fifth Arabian Horticulture Conference, Ismailia, Egypt, March 2001, pp.
24–28, and pp. 133–140.
[9] M. Abdel-Mouty, H. Mona Ali, A. Nisha, and A. Rizk, “Potato yield as
affected by interaction between bio-and organic fertilizers”. Egypt. J.
Appl. Sci., vol. 16, 2002, pp. 267–276.
[10] F. M. Gadallah, and T. A. El-Masry, “Onion growth and yield as
affected by bio-fertilization”. Annals Agric. Sci. Moshtohor, vol. 44,
2006, pp. 987–1005.
[11] A. Sh. Osman, “Effect of partial substitution of mineral-N by
biofertilization on growth, yield and yield components of potato”. The
3rd Conf. Sustain. Agric. Develop., Fac. Agric., Fayoum Univ., 12–14
Nov. 2007, pp. 381–396.
[12] S. A. Ashour, A. E. Abdel-Fattah, and A. A. Tawfik, “Effect of nitrobin
(biofertilizer) and different levels of nitrogen on growth and yield of
potato (Solanum tuberosum, L.)”. J. Agric. Sci., Mansoura Univ., vol.
22, 1997, pp. 3979–3986.
[13] A. H. Hanafy, N. F. Kheir, and N. B. Talaat, “Physiological studies on
reducing the accumulation of nitrate in jew’s mallow (Corchorus
olitorius) and radish (Raphanus sativus L.)”. Bull. Fac. Agric., Cairo
Univ., vol. 48, 1997, pp. 25–64.
[14] S. Rozek, and R. Wojciechowska, “Effect of urea foliar application and
different levels of nitrogen in soil on broccoli head yield and its quality
in autumn growing cycle”. Sodininkyste-ir Darzininkyste, vol. 24, 2005,
pp. 291–301. (c.a., CAB Abst., 20063014208).
[15] R. Wojciechowska, S. Rozek, and A. Rydz, “Broccoli yield and its
quality in spring growing cycle as dependent on nitrogen fertilization”.
Folia-Horticulturae, vol. 17, 2005, pp. 141–152. (c.a. CAB Abst.
20063029819).
[16] C. Beecher, “Cancer preventive properties of varieties of Brassica
oleracea”. A Review, Amer. J. Clin. Nutri., vol. 59, 1994, pp. 1166–
1170.
[17] M. M. Abou El-Magd, A. M. El Bassiony, and Z. F. Fawzy, “Effect of
organic manure with or without chemical fertilizers on growth, yield and
quality of some varieties of broccoli plants”. J. Appl. Sci. Res., vol. 2,
2006, pp. 791–798.
[18] S. A. Wilde, R. B. Corey, J. G. Lyer, and G. K. Voigt, “Soil and plant
analysis for tree culture”. Oxford and IBM Publishers. New Delhi. India.
3rd Ed., 1985, pp. 93–106.
[19] M. S. Tolba, “Influence of different nitrogenous and potassic
fertilization levels on vegetative growth, heads yield and chemical
composition of broccoli (Brassica oleracea var. italica)”. Ph.D. Thesis,
Fac. Agric., Fayoum Branch, Cairo Univ, Egypt, 2005.
[20] N. A. Hegazi, and S. Niemela, “A note on the estimation of Azotobacter
density by membrane filter technique”. J. Appl. Bacteriol., vol. 41, 1976,
p. 311.
[21] J. Dobereiner, I. E. Marril, and M. Niery, “Ecological distribution of
Spirillum lipoferum, Beijerinek”. Can. J. Microbiol., vol. 22, 1976, pp.
1464–1473.
[22] A.O.A.C., “Association of Official Analytical Chemists. Official
Methods of Analysis”. 15th Ed., Washington, D.C., USA, 1995.
[23] M. L. Jackson, “Soil Chemical Analysis”. New Jersey Prentice-Hall,
Inc., 1967.
[24] A. I. Page, R. H. Miller, and D. R. Keeney, “Methods of Soil Analysis”.
Part 2: Chemical and Microbiological Properties. 2nd Ed., Amer. Soc. of
Agron., Madison, Wisconsin, USA, 1982.
[25] M. Radojevic, and N. V. Bashkin, “Practical Environmental Analysis”.
Royal Society of Chemistry and Thomas Graham House, Cambridge,
UK, 1999, pp. 180–430.
[26] La Motte, “Smart Spectro Water and Waste Water Procedure Analysis
Manual”. La Motte Inc., Washington, DC, USA, 2000, pp. 68–180.
[27] SAS, “SAS/STAT User's Guide”. Version 6.12. SAS Institute, Cary,
NC, USA, 1996. (http://www.sas.com/technologies/analytics/
statistics/stat/index.html).
[28] G. W. Snedecor, and W. G. Cochran, “Statistical Methods”. Oxford and
J. B. H. Publ. Com. 7th ed., 1980.
[29] S. Singh, and K. K. Kapoor, “Inoculation with phosphate-solubilizing
microorganisms and visicular-arbuscular mycorrhizal fungus improves
dry matter yield and nutrient uptake by wheat grown in sandy soil”.
Biol. Ferti. Soils, vol. 28, 1999, pp. 139–144.
[30] J. Balasubramanian, “Studies on the combined effect of Azospirillium
VA mycorrhizal and inorganic fertilizers on growth performance of
French marigold (Tegetes putula L.)”. SIH, vol. 37, 1989, p. 311.
[31] M. S. Premkumari, and A. Balasubramanian, “Effect of combined
inoculation of VAM and Azospirillum on growth and nutrient uptake of
coffee seedlings”. Indian Coffee, vol. 57, 1993, pp. 5–11.
[32] K. J. Vessey, “Plant growth promoting rhizobacteria as biofertilizer”.
Plant Soil, vol. 255, 2003, pp. 571–586.
[33] M. Hemavathi, “Effect of organic manures and biofertilizers on growth
and productivity of chrysanthemum (Chrysanthemum molifolium
Ramat.) cv. Local yellow”. M.Sc.(Agri.) Thesis, Univ. Agric. Sci.,
Bangalore, 1997.
[34] B. M. Shubha, “Integrated nutrient management for growth, flowering
and xanthophylls Yield of marigold (Tagetes erecta L.)”. M.Sc.(Agric.)
Thesis, Univ. Agric. Sci., Dharwad, 2006.
[35] E. Mahmoud, N. Abd El-Kader, P. Robin, N. Akkal-Corfini, and L. Abd
El-Rahman, “Effects of different organic and inorganic fertilizers on
cucumber yield and some soil properties”. World J. Agric. Sci., vol. 5,
2009, pp. 408–414.
[36] B. S. Kundu, and A. C. Gaur, “Effect of phosphor bacteria on the yield
and phosphate Uptake of potato crop”. Current Sci., vol. 49, 1980, pp.
159–160.</p>
<p>[1] A. Hartman, “Ecophysiological aspects of growth and nitrogen fixation
in Azospirillium species”. Plant Soil, vol. 110, 1988, pp. 225–238.
[2] N. P. Singh, R. S. Sachan, P. C. Pandey, and P. S. Bisht, “Effect of a
decade long fertilizer and manure application on soil fertility and
productivity of rice-wheat system in Molisols”. J. Indian Soc. Soil Sci.,
vol. 47, 1999, pp. 72–80.
[3] A. Bhatia, H. Pathak, and H. C. Joshi, “Use of sewage as a source of
plant nutrient: potentials and problems”. Fert. News, vol. 46, 2001, pp.
55–58.
[4] C. A. Palm, C. N. Gachengo, R. J. Delve, G. Cadisch, and K. E. Giller,
“Organic inputs for soil fertility management in tropical agro
ecosystems: application of an organic resource database”. Agric.
Ecosyst. Environ, vol. 83, 2001, pp. 27–42.
[5] E. C. Cocking, “Helping plants get more nitrogen from air”. European
Review, vol. 8, 2000, pp. 193–200.
[6] A. N. Ibrahim, and I. M. Abd El-Aziz, “Solubilization of rock phosphate
by streptomyces”. Agr. Talajton, vol. 26, 1977, pp. 424–434.
[7] M. Fayez, N. F. Eman, and H. E. Makbol, “The possible use of nitrogen
fixing Azospirillum as biofertilizer for wheat plants”. Egypt. J.
Microbiol., vol. 20, 1985, pp. 199–206.
[8] M. R. Abdel-Latif, A. A. El-Bana, and A. A. Galal, “Effect of
biofertilizers Microbein and Phosphorine on bacterial pod blight of guar
and black cumin damping off root rot and with diseases”. Proc. of the
fifth Arabian Horticulture Conference, Ismailia, Egypt, March 2001, pp.
24–28, and pp. 133–140.
[9] M. Abdel-Mouty, H. Mona Ali, A. Nisha, and A. Rizk, “Potato yield as
affected by interaction between bio-and organic fertilizers”. Egypt. J.
Appl. Sci., vol. 16, 2002, pp. 267–276.
[10] F. M. Gadallah, and T. A. El-Masry, “Onion growth and yield as
affected by bio-fertilization”. Annals Agric. Sci. Moshtohor, vol. 44,
2006, pp. 987–1005.
[11] A. Sh. Osman, “Effect of partial substitution of mineral-N by
biofertilization on growth, yield and yield components of potato”. The
3rd Conf. Sustain. Agric. Develop., Fac. Agric., Fayoum Univ., 12–14
Nov. 2007, pp. 381–396.
[12] S. A. Ashour, A. E. Abdel-Fattah, and A. A. Tawfik, “Effect of nitrobin
(biofertilizer) and different levels of nitrogen on growth and yield of
potato (Solanum tuberosum, L.)”. J. Agric. Sci., Mansoura Univ., vol.
22, 1997, pp. 3979–3986.
[13] A. H. Hanafy, N. F. Kheir, and N. B. Talaat, “Physiological studies on
reducing the accumulation of nitrate in jew’s mallow (Corchorus
olitorius) and radish (Raphanus sativus L.)”. Bull. Fac. Agric., Cairo
Univ., vol. 48, 1997, pp. 25–64.
[14] S. Rozek, and R. Wojciechowska, “Effect of urea foliar application and
different levels of nitrogen in soil on broccoli head yield and its quality
in autumn growing cycle”. Sodininkyste-ir Darzininkyste, vol. 24, 2005,
pp. 291–301. (c.a., CAB Abst., 20063014208).
[15] R. Wojciechowska, S. Rozek, and A. Rydz, “Broccoli yield and its
quality in spring growing cycle as dependent on nitrogen fertilization”.
Folia-Horticulturae, vol. 17, 2005, pp. 141–152. (c.a. CAB Abst.
20063029819).
[16] C. Beecher, “Cancer preventive properties of varieties of Brassica
oleracea”. A Review, Amer. J. Clin. Nutri., vol. 59, 1994, pp. 1166–
1170.
[17] M. M. Abou El-Magd, A. M. El Bassiony, and Z. F. Fawzy, “Effect of
organic manure with or without chemical fertilizers on growth, yield and
quality of some varieties of broccoli plants”. J. Appl. Sci. Res., vol. 2,
2006, pp. 791–798.
[18] S. A. Wilde, R. B. Corey, J. G. Lyer, and G. K. Voigt, “Soil and plant
analysis for tree culture”. Oxford and IBM Publishers. New Delhi. India.
3rd Ed., 1985, pp. 93–106.
[19] M. S. Tolba, “Influence of different nitrogenous and potassic
fertilization levels on vegetative growth, heads yield and chemical
composition of broccoli (Brassica oleracea var. italica)”. Ph.D. Thesis,
Fac. Agric., Fayoum Branch, Cairo Univ, Egypt, 2005.
[20] N. A. Hegazi, and S. Niemela, “A note on the estimation of Azotobacter
density by membrane filter technique”. J. Appl. Bacteriol., vol. 41, 1976,
p. 311.
[21] J. Dobereiner, I. E. Marril, and M. Niery, “Ecological distribution of
Spirillum lipoferum, Beijerinek”. Can. J. Microbiol., vol. 22, 1976, pp.
1464–1473.
[22] A.O.A.C., “Association of Official Analytical Chemists. Official
Methods of Analysis”. 15th Ed., Washington, D.C., USA, 1995.
[23] M. L. Jackson, “Soil Chemical Analysis”. New Jersey Prentice-Hall,
Inc., 1967.
[24] A. I. Page, R. H. Miller, and D. R. Keeney, “Methods of Soil Analysis”.
Part 2: Chemical and Microbiological Properties. 2nd Ed., Amer. Soc. of
Agron., Madison, Wisconsin, USA, 1982.
[25] M. Radojevic, and N. V. Bashkin, “Practical Environmental Analysis”.
Royal Society of Chemistry and Thomas Graham House, Cambridge,
UK, 1999, pp. 180–430.
[26] La Motte, “Smart Spectro Water and Waste Water Procedure Analysis
Manual”. La Motte Inc., Washington, DC, USA, 2000, pp. 68–180.
[27] SAS, “SAS/STAT User's Guide”. Version 6.12. SAS Institute, Cary,
NC, USA, 1996. (http://www.sas.com/technologies/analytics/
statistics/stat/index.html).
[28] G. W. Snedecor, and W. G. Cochran, “Statistical Methods”. Oxford and
J. B. H. Publ. Com. 7th ed., 1980.
[29] S. Singh, and K. K. Kapoor, “Inoculation with phosphate-solubilizing
microorganisms and visicular-arbuscular mycorrhizal fungus improves
dry matter yield and nutrient uptake by wheat grown in sandy soil”.
Biol. Ferti. Soils, vol. 28, 1999, pp. 139–144.
[30] J. Balasubramanian, “Studies on the combined effect of Azospirillium
VA mycorrhizal and inorganic fertilizers on growth performance of
French marigold (Tegetes putula L.)”. SIH, vol. 37, 1989, p. 311.
[31] M. S. Premkumari, and A. Balasubramanian, “Effect of combined
inoculation of VAM and Azospirillum on growth and nutrient uptake of
coffee seedlings”. Indian Coffee, vol. 57, 1993, pp. 5–11.
[32] K. J. Vessey, “Plant growth promoting rhizobacteria as biofertilizer”.
Plant Soil, vol. 255, 2003, pp. 571–586.
[33] M. Hemavathi, “Effect of organic manures and biofertilizers on growth
and productivity of chrysanthemum (Chrysanthemum molifolium
Ramat.) cv. Local yellow”. M.Sc.(Agri.) Thesis, Univ. Agric. Sci.,
Bangalore, 1997.
[34] B. M. Shubha, “Integrated nutrient management for growth, flowering
and xanthophylls Yield of marigold (Tagetes erecta L.)”. M.Sc.(Agric.)
Thesis, Univ. Agric. Sci., Dharwad, 2006.
[35] E. Mahmoud, N. Abd El-Kader, P. Robin, N. Akkal-Corfini, and L. Abd
El-Rahman, “Effects of different organic and inorganic fertilizers on
cucumber yield and some soil properties”. World J. Agric. Sci., vol. 5,
2009, pp. 408–414.
[36] B. S. Kundu, and A. C. Gaur, “Effect of phosphor bacteria on the yield
and phosphate Uptake of potato crop”. Current Sci., vol. 49, 1980, pp.
159–160.</p>
@article{"International Journal of Biological, Life and Agricultural Sciences:59566", author = "Saad M. Howladar and Mostafa M. Rady and Ashraf Sh. Osman", title = "Effect of Bio-Nitrogen as a Partial Alternative to Mineral-Nitrogen Fertiliser on Growth, Nitrate and Nitrite Contents, and Yield Quality in Brassica oleracea L.", abstract = "Effects of bio-nitrogen fertilizer (bio-N), as a partial
alternative to mineral-nitrogen fertilizer (mineral-N), on growth,
yield and yield quality of broccoli plants were investigated. Bio-N
was applied at 1, 2 or 3 doses in combination with 65% of the
recommended dose of mineral-N (bio-N1, bio-N2 or bio-N3 +
⅔mineral-N). However, 100% of the recommended dose of mineral-
N was applied as a control. Significant positive influences of the bio-
N3 + ⅔mineral-N treatment were observed on growth traits, leaf
contents of nitrogen, phosphorus, potassium, nitrate and nitrite, and
yield quality when compared to the other two combined treatments.
In contrast, there were no significant differences in these parameters
between the bio-N3 + ⅔mineral-N and the control treatments, except
for leaf contents of nitrate and nitrite. They showed lower contents in
the bio-N3 + ⅔mineral-N treatment than the control. Therefore, we
recommend using bio-N as a partial alternative to mineral-N for
healthy nutrition.
", keywords = "Bio-fertilization, broccoli, growth, nitrate, nitrite,
yield quality.", volume = "7", number = "7", pages = "625-6", }
