Detection of Tetracycline Resistance Genes in Lactococcus garvieae Strains Isolated from Rainbow Trout

The present study was done to evaluate the presence
of tetracycline resistance genes in Lactococcus garvieae isolated
from cultured rainbow trout, West Iran. The isolates were examined
for antimicrobial resistance using disc diffusion method. Of the 49
strains tested, 19 were resistant to tetracycline (38.7%), 32 to
enrofloxacin (65.3%), 21 to erythromycin (42.8%), 20 to
chloramphenicol and trimetoprim-sulfamethoxazole (40.8%). The
strains were then characterized for their genotypic resistance profiles.
The results revealed that all 49 isolates contained at least one of the
tetracycline resistance genes. Tet (A) was found in 89.4% of
tetracycline resistant isolates and the frequency of other gene were as
follows: tet (E) 42.1%, tet (B) 47.3%, tet (D) 15.7%, tet (L) 26.3%,
tet (K) 52.6%, tet (G) 36.8%, tet (34) 21%, tet (S) 63.1%, tet (C)
57.8%, tet (M) 73.6%, tet (O) 42.1%. The results revealed high levels
of antibiotic resistance in L. garvieae strains which is a potential
danger for trout culture as well as for public health.

• M. Raissy
• M. Shahrani

• Lactococcus garvieae
• rainbow trout
• tetracycline resistance genes.

[1] M. Raissy, and M. Ansari, “Antibiotic susceptibility of Lactococcus
garvieae isolated from rainbow trout (Oncorhynchus mykiss)”. Afr. J.
Biotechnol, vol. 8, 2011, pp. 1473-1476.
[2] M.D. Collins, C. Ash, J.A.E. Farrow, S. Wallbanks, and A.M.
Williams,“16S ribosomal ribonucleic acid sequence analyses of
lactococci and related taxa. Description of Vagococcusfluvialis gen.
nov.sp. nov”. J. Appl. Bacteriol, vol. 67, 1989, pp. 453-460J.
[3] N. Carson, B.Gudkovs, and B. Austin, “Characteristics of an
Enterococcus-like bacterium from Australia and South Africa,
pathogenic for rainbow trout (Oncorhynchus mykiss Walbaum)”. J. Fish.
Dis. vol. 6, 1993: 381-8.
[4] A. Eldar, C. Ghittino, L. Asanta, E. Bozzetta, M. Goria, M. Prearo, and
H. Bercovier, “Enterococcus seriolicida is a junior synonym
of Lactococcus garvieae, a causative agent of septicemia and
meningoencephalitis in fish. Current”. Microbiol, vol. 32, 1996, pp. 85-
88.
[5] J.A. Elliott, M.D. Collins, N.E. Pigott, and R.R. Facklam,
“Differentiation of Lactococcus lactis and Lactococcus garvieae from
humans by comparison of whole-cell protein patterns”. J. Clin.
Microbiol, vol. 29, 1991, pp. 2731-2734.
[6] C.Y. Wang, H.S. Shie, S.C. Chen, J.P. Huang, I.C. Hsieh, M.S. Wen,
F.C. Lin, D. Wu,“Lactococcus garvieae infections in humans: possible
association with aquaculture outbreaks”. Int. J. Clin. Prac, vol. 61, 2007,
pp. 68-73.
[7] P.H. Chang, C.W. Lin, and Y.C. Lee, “Lactococcus garvieae infection
of cultured rainbow trout, Oncorhynchus mykiss, in Taiwan and
associated biophysical characteristics and histopathology”. Bull. Euro.
Assoc. Fish Pathol. Vol. 22, 2002, pp. 319-27.
[8] S.C. Chen, Y.D. Lin, L.L. Liaw, and P.C. Wang,“Lactococcus garvieae
infection in the giant freshwater prawn Macrobranchium rosenbergii
confirmed by polymerase chain reaction and 16S rDNA sequencing”.
Dis. Aqua. Organs, vol. 45, 2001, pp. 45-52.
[9] S.C. Chen, L.L. Liaw, H.Y. Su, S.C. Ko, C.Y. Wu, H.C. Chaung, Y.H.
Tsai, K.L. Yang, Y.C. Chen, T.H. Chen, G.R. Lin, S.Y. Cheng, Y.D.
Lin, J.L. Lee, C.C. Lai, Y.J. Weng, and S.Y. Chu,“Lactococcus
garvieae, a cause of disease in grey mullet, Mugilcephalus L. in
Taiwan”. J. Fish Dis, vol. 25, 2002, pp. 727-232.
[10] O. Diler, S. Altun, A.K. Adiloglu, A. Kubilay, and B. Isikli, “First
occurrence of Streptococcosis affecting farmed rainbow trout in
Turkey”. Bull. Europ. Assoc. Fish Pathol, vol. 22, 2002, pp. 21-26.
[11] M.A. Palacios, M.J. Zamora, J. VaSquez Zamora, and A. Duran,
“Streptococcosis in rainbow trout (Oncorhynchus mykiss) in Spain”.
Boll. Soc. Ital. Patalog. Itt, vol. 13, 1993, pp.11-6.
[12] M. Soltani, G.H. Nikbatht, H. Mousavi, and N. Ahmadzadeh, “Epizootic
outbreak of lactococcosis caused by Lactococcus garvieae in farmed
rainbow trout (Oncorhynchus mykiss) in Iran”. Bull. Europ. Assoc. Fish
Pathol, vol. 28, 2008, pp. 207-212.
[13] E. Charpentier, G. Gerbaud, and P. Courvalin, “Characterization of a
new class of tetracycline-resistance gene tet(S)in Listeria
monocytogenesBM4210”. Gene, vol. 131, 1993, pp. 27-34.
[14] H. Sharifiyazdi, M. Akhlaghi, M. Tabatabaei, and S.M. MostafaviZadeh,
“Isolation and characterization of Lactococcus garvieae from diseased
rainbow trout (Oncorhynchus mykiss, Walbaum) cultured in Iran”. Iran.
J. Vet. Res, vol. 11, 2010, pp. 342-350.
[15] B. Austin, D.A. Austin, “Bacterial Fish Pathogens: Disease of Farmed
and Wild Fish”, 4th ed. Bristol: Springer, 2007. [16] A. Zlotkin, A. Eldar, C. Ghittino, H. Bercovier, “Identification of
Lactococcus garvieae by PCR”. J. Clin. Microbiol, vol. 36, 1998, pp.
983-985.
[17] CLSI, Clinical and Laboratory Standards Institute. 2007: “Performance
Standards for Antimicrobial Susceptibility Testing; Fifteenth
Informational Supplement”. CLSI document M100-S15. Clinical and
Laboratory Standards Institute. Wayne, Pennsylvania.
[18] K. Kav, and O. Erganis. “Antibiotic Susceptibility of Lactococcus
garvieae in rainbow trout (Oncorhyncus mykiss) Farms”. Bull. Vet.
InsPul, vol. 52, 2008, pp. 223-226.
[19] C. Walther, A. Rossano, A. Thomann, and V. Perreten, “Antibiotic
resistance in Lactococcus species from bovine milk: Presence of a
mutated multidrug transporter mdt(A) gene in susceptible Lactococcus
garvieae strains”. Vet. Microbiol, vol. 131, 2008, pp. 348-357.
[20] P. Alves D’azevedo, C. Perin, F.L. Becker, and G.Z Ramos, “Isolation
and characterization of Lactococcus garvieae”. Rev. Amr. Porto. Alegra.
vol 44, 2000, pp. 81-84.
[21] S.R. Kim, L. Lisa, and S. Suzuki, “Occurrence of tetracycline resistance
genes tet(M) and tet(S)in bacteria from marine aquaculture sites”. FEMS.
Microbiol. Lett, vol 237, 2004, pp. 147-156.
[22] O.G. Kim, O.H. Mitsuko, and S. Suzuk, “Occurrence of Tetracycline
Resistant Bacteria and tet(M) Gene in Seawater from Korean Coast”.
Environ. Poll. Ecotoxicol, vol. 6, 2012, pp. 367-375.