Comparative Growth Rates of Treculia africana Decne: Embryo in Varied Strengths of Murashige and Skoog Basal Medium

This study provides a regeneration protocol for Treculia africana Decne (an endangered plant) through embryo culture. Mature zygotic embryos of T. africana were excised from the seeds aseptically and cultured on varied strengths (full, half and quarter) of Murashige and Skoog (MS) basal medium supplemented. All treatments experienced 100±0.00 percent sprouting except for half and quarter strengths. Plantlets in MS full strength had the highest fresh weight, leaf area, and longest shoot length when compared to other treatments. All explants in full, half, quarter strengths and control had the same number of leaves and sprout rate. Between the treatments, there was a significant difference (P>0.05) in their effect on the length of shoot and root, number of adventitious root, leaf area, and fresh weight. Full strength had the highest mean value in all the above-mentioned parameters and differed significantly (P>0.05) from others except in shoot length, number of adventitious roots, and root length where it did not differ (P<0.05) from half strength. The result of this study indicates that full strength MS basal medium offers a better option for the optimum growth for Treculia africana regeneration in vitro.

Authors:

• Okafor C. Uche
• Agbo P. Ejiofor
• Okezie C. Eziuche

Keywords:

• Medium strengths
• Murashige and Skoog
• Treculia africana
• zygotic embryos.

References:

[1] Agbogidi, O. M. and Onomeregbor, V. A. (2008). Morphological changes in the seedlings of Treculia africana grown in crude oil impacted soils. In: Popoola, L. (ed.). Climate Change and Sustainable Renewable Natural Resources Management. Proceedings of 32nd Annual Conference of the Forestry Association of Nigeria. Umuahia, Abia-State, Nigeria. 20th – 24th Oct; 2008, pp.170-182.
[2] Osuji, J. O. and Owei, S. D. (2010). Mitotic index studies on Treculia africana Decne. in Nigeria. Australian Journal of Agricultural Engineering, 1 (1): 25-28.
[3] Nuga, O.O. and Ofodile, E.A.U. (2010). Potentials of Treculia Africana Decne- An endangered species of Southern Nigeria. Journal of Agriculture and Social Research 10(2): 91-99.
[4] Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473–497.
[5] Aghimien, E. V., Kukogho, J., Ojo, M. O., Adams, B. A. and Akinbosoye, B. S. (2011). Effect of poultry manure, agrosorb and watering regime on the growth of Treculia africana (Decne.) seedlings. Continental Journal of Agronomy, 5 (2): 15 - 21.
[6] Idowu, O. J., Olanite, J. A., Arigbede, O. M., Adedire, M. O., Adeoye, S. A. and Adelusi, O. O. (2013). Effect of storage methods on germination, growth and proximate composition of Treculia africana var. decne(dis cannot be a variety) seedlings. Journal of Agriculture and Biodiversity Research, 2 (6): 117-123.
[7] Oboho, E. G. and Ngalum, E. L. (2014). Germination response of Treculia africana Decne. seeds in relation to moisture content, storage method and its duration. Journal of Applied and Natural Science 6 (1): 88-94.
[8] Edwin, R. (2005). Biology-Botany. Tamil Nadu Textbook Corporation, Chennai India, 216pp.
[9] Pierik, R. L. M. (1987). In vitro Culture of Higher Plants. Martinas Nijkost publishers, wageningen. 344 pp.
[10] Melita, M., Ram, R. and Bhattacharya, A. (2014). A simple and cost effective liquid culture system for the micropropagtion of two commercially important apple rootstocks. Indian Journal of Experimental Biology, 52: 748-754.
[11] Shreeti, P., Tripti, R., Gaurav, P. and Bijaya, P. (2013). Effect of different media on in vitro seed germination and seedling development of Cymbidium aloifolium (L.) Sw. Nepal Journal of Science and Technology, 14 (1): 51-56.
[12] Yildirim, H., Tilkat, E., Onay, A. and Ozen, H. C. (2007). In vitro Embryo Culture of Apricot, Prunus armeniaca L. CV. International Journal of Science and Technology, 2:99-104.
[13] Samuel, K., Debashish, D., Madhumita, B., Padmaja, G., Prasad, S. R., Murthy, V. B. R. and Rao, P. S. (2009). In vitro germination and micropropagation of Givotia rottleriformis Griff. In Vitro Cell Developmental Biology Plant, 5: 466-473.