Codon-optimized Carbonic Anhydrase from Dunaliella species: Expression and Characterization
Carbonic anhydrases (CAs) has been focused as
biological catalysis for CO2 sequestration process because it can
catalyze the conversion of CO2 to bicarbonate. Here, codon-optimized
sequence of α type-CA cloned from Duneliala species. (DsCAopt) was
constructed, expressed, and characterized. The expression level in E.
coli BL21(DE3) was better for codon-optimized DsCAopt than intact
sequence of DsCAopt. DsCAopt enzyme shows high-stability at pH
7.6/10.0. In final, we demonstrated that in the Ca2+ solution, DsCAopt
enzyme can catalyze well the conversion of CO2 to CaCO3, as the
calcite form.
[1] Puxty, G., Rowland, R., Allport, A., Yang, Q., Bown, M., Burns, R.,
Maeder, M., and Attalla, M. (2009) Carbon dioxide postcombustion
capture: a novel screening study of the carbon dioxide absorption
performance of 76 amines. Environ. Sci. Technol. 43, 6427-6433.
[2] Veawab, A., Tontiwachwuthikul, P., and Chakma, A. (1999) Corrosion
behavior of carbon steel in the CO2 absorption process using aqueous
amine solutions.Ind. Eng. Chem. Res. 38, 3917-3924.
[3] Dawson, B., and Spannagle, M. (2009) The Complete Guide to Climate
Change, 1st ed., Routledge, NewYork, USA
[4] Lee, S.W., Park, S.B., Jeong, S.K., Limc, K.S., Lee, S.H. and
Trachtenberg, M.C.(2010) On carbon dioxide storage based on
biomineralization strategies. Micron 41, 273-282.
[5] Frommer, W.B. (2010) CO2mmon Sense. Science 327, 275-276.
[6] Figueroa, J.D., Fout, T., Plasynski, S., McIlvried, H., and Srivastava, R.D.
(2008) Advances in CO2 capture technologyÔÇöThe U.S. department of
energy's carbon sequestration program.Int. J. Green-House Gas Control
2, 9-20.
[7] Puskas, L.G., Inui, M., Zahan, K., and Yukawa, H. (2000) A periplasmic,
╬▒-type carbonic anhydrase from Rhodopseudomonas palustris is essential
for bicarbonate uptake. Microbiology 146, 2957-2966.
[8] Smith, K.S., and Ferry, J.G. (2000) Prokaryotic carbonic anhydrases .
FEMS Microbiol. Rev. 24, 335-366.
[9] Tripp, B.C., Smith, K., and Ferry, J.G. (2001) Carbonic anhydrase: new
insights for an ancient enzyme. J. Biol. Chem. 276, 48615-48618.
[10] So, A.K., Espie, G.S., Williams, E.B., Shively, J.M., Heinhorst, S., and
Cannon, G.C. (2004) A novel evolutionary lineage of carbonic anhydrase
(╬Á class) is a component of the carboxysome shell. J. Bacteriol. 186,
623-630.
[11] Madern, D., Ebel, C., and Zaccai, G. (2000) Halophilic adaptation of
enzymes.Extremophiles 4, 91-98.
[12] Mevarech, M., Frolow, F., and Gloss, L.M. (2000) Halophilic enzymes:
proteins with a grain of salt. Biophys. Chem. 86, 155-164.
[13] Szilagyi F.A.U.A., and Zavodszky, P. (2000) Structural differences
between mesophilic, moderately thermophilic and extremely
thermophilic protein subunits: results of a comprehensive survey. Struct.
Fold Des. 8, 493-504.
[14] Borowitzka, M.A., and Borowitzka, L.J. (1988). Micro-algal
Biotechnology Cambridge University Press, New York, USA.
[15] Fisher, M., Pick, U., and Zamir, A. (1994) A salt-induced 60-kilodalton
plasma membrane protein plays a potential role in the extreme
halotolerance of the alga Dunaliella. Plant Physiol. 106, 1359-1365.
[16] Sambrook, J., and Russel, D.W. (2001). Molecular cloning: A laboratory
manual, 3rd ed, Cold Spring Harbor labora-tory Press, Cold Spring
Harbor, New York, USA.
[17] Laemmli, U. K. (1970) Cleavage of structural proteins during the
assembly of the head of bacteriophage T4. Nature 227, 680-685.
[18] Bradford, M. M. (1976) A rapid and sensitive method for the quantitation
of microgram quantities of protein utilizing the principle of protein-dye
binding Anal. Biochem. 72, 248-254.
[19] Rickli, E.E., Ghazanfar, S.A.S., Gibbons, B.H., and Edsall, J.T. (1964)
Carbonic anhydrases from human erythrocytes: preparation and
properties of two enzymes. J. Biol.Chem. 239, 1065-1078.
[20] Verpoorte, J. A.; Mehta, S., and Edsall, J. T. (1967) Esterase activities of
human carbonic anhydrases B and C. J. Biol. Chem. 242, 4221-4229.
[21] Mirjafari, P., Asghari, K., and Mahinpey, N., (2007) Investigating the
application of enzyme carbonic anhydrase for CO2 sequestration
purposes. Ind. Eng. Chem. Res. 46, 921-926.
[22] Sharma, A., and Bhattacharya, (2010) Enhanced biomimetic
sequestration of CO2 into CaCO3 using purified carbonic anhydrase from
indigenous bacterial strains. J. Mol. Catal. B-Enzym. 67, 122-128.
[23] Lineweaver, H., and Burk, D. (1934) The determination of enzyme
dissociation constants. J. Am. Chem. Soc. 57, 658-666.
[24] Hakan, S., and Beydemir, S. (2011) The impact of heavy metals on the
activity of carbonic anhydrase from rainbow trout (Oncorhynchus mykiss)
kidney.Toxicol. Ind. Health, 28, 296-305.
[25] Ceyhun, S. B., Sent├╝rk, M., Yerlikaya, Emrah, Erdogan, O., K├╝frevioglu,
Ö. I., and Ekinci, D. (2011) Purification and characterization of carbonic
anhydrase from the teleost fish Dicentrarchus labrax (European seabass)
liver and toxicological effects of metals on enzyme activity. Environ.
Toxicol. Phar.32, 69-74.
[26] Ekinci, D., Ceyhun S. B., Sentürk, M., Erdem, D., Küfrevioglu, Ö. I. and
Supuran, C. T. (2011) Characterization and anions inhibition studies of
an ╬▒-carbonic anhydrase from the teleost fish Dicentrarchus
labrax .Bioorgan. Med. Chem. 19,744-748
[27] Kupriyanova, E., Villarejo. A., Markelova, A., Gerasimenko, L., Zavarzin,
G., Samuelsson, G., Los, D.A. and Pronina, N. (2007) Extracellular
carbonic anhydrases of the stromatolite-forming cyanobacterium
Microcoleus chthonoplastes. Microbiology 153, 1149-1156.
[28] Stahler, M.F., Ganter, L., Katherin, L., Manfred, K., and Stephen, B.
(2005) Mutational analysis of the Helicobacter pylori carbonic
anhydrases. FEMS Immunol. Med. Microbiol. 44, 183-189.
[29] de Leeuw, N.H., and Parker, S.C. (1998) Surface structure and
morphology of calcium carbonate polymorphs calcite, aragonite, and
vaterite: an atomistic approach. J. Phys. Chem. B 102, 384, 2914-2922.
[30] Ouhenia, S., Chateigner, D., Belkhir, M.A., Guilmeau, E. and Krauss, C.
(2008) Synthesis of calcium carbonate polymorphs in the presence of
polyacrylic acid. J. Cryst. Growth 310, 2832 -2841.
[31] Garcia-Carmona, J., Morales, J.G., and Clemente, R.R. (2003)
Morphological control of precipitated calcite obtained by adjusting the
electrical conductivity in the Ca(OH)2-H2O-CO2 system . J. Cryst.
Growth 249, 561-571.
[32] Vinoba, M., Kim, D. H., Lim, K. S., Jeong, S. K., Lee, S. W. and Alagar,
M. (2011) Biomimetic sequestration of CO2 and reformation to CACO3
using bovine carbonic anhydrase immobilized on SBA-15. Energy Fuels
25, 438-445.
[1] Puxty, G., Rowland, R., Allport, A., Yang, Q., Bown, M., Burns, R.,
Maeder, M., and Attalla, M. (2009) Carbon dioxide postcombustion
capture: a novel screening study of the carbon dioxide absorption
performance of 76 amines. Environ. Sci. Technol. 43, 6427-6433.
[2] Veawab, A., Tontiwachwuthikul, P., and Chakma, A. (1999) Corrosion
behavior of carbon steel in the CO2 absorption process using aqueous
amine solutions.Ind. Eng. Chem. Res. 38, 3917-3924.
[3] Dawson, B., and Spannagle, M. (2009) The Complete Guide to Climate
Change, 1st ed., Routledge, NewYork, USA
[4] Lee, S.W., Park, S.B., Jeong, S.K., Limc, K.S., Lee, S.H. and
Trachtenberg, M.C.(2010) On carbon dioxide storage based on
biomineralization strategies. Micron 41, 273-282.
[5] Frommer, W.B. (2010) CO2mmon Sense. Science 327, 275-276.
[6] Figueroa, J.D., Fout, T., Plasynski, S., McIlvried, H., and Srivastava, R.D.
(2008) Advances in CO2 capture technologyÔÇöThe U.S. department of
energy's carbon sequestration program.Int. J. Green-House Gas Control
2, 9-20.
[7] Puskas, L.G., Inui, M., Zahan, K., and Yukawa, H. (2000) A periplasmic,
╬▒-type carbonic anhydrase from Rhodopseudomonas palustris is essential
for bicarbonate uptake. Microbiology 146, 2957-2966.
[8] Smith, K.S., and Ferry, J.G. (2000) Prokaryotic carbonic anhydrases .
FEMS Microbiol. Rev. 24, 335-366.
[9] Tripp, B.C., Smith, K., and Ferry, J.G. (2001) Carbonic anhydrase: new
insights for an ancient enzyme. J. Biol. Chem. 276, 48615-48618.
[10] So, A.K., Espie, G.S., Williams, E.B., Shively, J.M., Heinhorst, S., and
Cannon, G.C. (2004) A novel evolutionary lineage of carbonic anhydrase
(╬Á class) is a component of the carboxysome shell. J. Bacteriol. 186,
623-630.
[11] Madern, D., Ebel, C., and Zaccai, G. (2000) Halophilic adaptation of
enzymes.Extremophiles 4, 91-98.
[12] Mevarech, M., Frolow, F., and Gloss, L.M. (2000) Halophilic enzymes:
proteins with a grain of salt. Biophys. Chem. 86, 155-164.
[13] Szilagyi F.A.U.A., and Zavodszky, P. (2000) Structural differences
between mesophilic, moderately thermophilic and extremely
thermophilic protein subunits: results of a comprehensive survey. Struct.
Fold Des. 8, 493-504.
[14] Borowitzka, M.A., and Borowitzka, L.J. (1988). Micro-algal
Biotechnology Cambridge University Press, New York, USA.
[15] Fisher, M., Pick, U., and Zamir, A. (1994) A salt-induced 60-kilodalton
plasma membrane protein plays a potential role in the extreme
halotolerance of the alga Dunaliella. Plant Physiol. 106, 1359-1365.
[16] Sambrook, J., and Russel, D.W. (2001). Molecular cloning: A laboratory
manual, 3rd ed, Cold Spring Harbor labora-tory Press, Cold Spring
Harbor, New York, USA.
[17] Laemmli, U. K. (1970) Cleavage of structural proteins during the
assembly of the head of bacteriophage T4. Nature 227, 680-685.
[18] Bradford, M. M. (1976) A rapid and sensitive method for the quantitation
of microgram quantities of protein utilizing the principle of protein-dye
binding Anal. Biochem. 72, 248-254.
[19] Rickli, E.E., Ghazanfar, S.A.S., Gibbons, B.H., and Edsall, J.T. (1964)
Carbonic anhydrases from human erythrocytes: preparation and
properties of two enzymes. J. Biol.Chem. 239, 1065-1078.
[20] Verpoorte, J. A.; Mehta, S., and Edsall, J. T. (1967) Esterase activities of
human carbonic anhydrases B and C. J. Biol. Chem. 242, 4221-4229.
[21] Mirjafari, P., Asghari, K., and Mahinpey, N., (2007) Investigating the
application of enzyme carbonic anhydrase for CO2 sequestration
purposes. Ind. Eng. Chem. Res. 46, 921-926.
[22] Sharma, A., and Bhattacharya, (2010) Enhanced biomimetic
sequestration of CO2 into CaCO3 using purified carbonic anhydrase from
indigenous bacterial strains. J. Mol. Catal. B-Enzym. 67, 122-128.
[23] Lineweaver, H., and Burk, D. (1934) The determination of enzyme
dissociation constants. J. Am. Chem. Soc. 57, 658-666.
[24] Hakan, S., and Beydemir, S. (2011) The impact of heavy metals on the
activity of carbonic anhydrase from rainbow trout (Oncorhynchus mykiss)
kidney.Toxicol. Ind. Health, 28, 296-305.
[25] Ceyhun, S. B., Sent├╝rk, M., Yerlikaya, Emrah, Erdogan, O., K├╝frevioglu,
Ö. I., and Ekinci, D. (2011) Purification and characterization of carbonic
anhydrase from the teleost fish Dicentrarchus labrax (European seabass)
liver and toxicological effects of metals on enzyme activity. Environ.
Toxicol. Phar.32, 69-74.
[26] Ekinci, D., Ceyhun S. B., Sentürk, M., Erdem, D., Küfrevioglu, Ö. I. and
Supuran, C. T. (2011) Characterization and anions inhibition studies of
an ╬▒-carbonic anhydrase from the teleost fish Dicentrarchus
labrax .Bioorgan. Med. Chem. 19,744-748
[27] Kupriyanova, E., Villarejo. A., Markelova, A., Gerasimenko, L., Zavarzin,
G., Samuelsson, G., Los, D.A. and Pronina, N. (2007) Extracellular
carbonic anhydrases of the stromatolite-forming cyanobacterium
Microcoleus chthonoplastes. Microbiology 153, 1149-1156.
[28] Stahler, M.F., Ganter, L., Katherin, L., Manfred, K., and Stephen, B.
(2005) Mutational analysis of the Helicobacter pylori carbonic
anhydrases. FEMS Immunol. Med. Microbiol. 44, 183-189.
[29] de Leeuw, N.H., and Parker, S.C. (1998) Surface structure and
morphology of calcium carbonate polymorphs calcite, aragonite, and
vaterite: an atomistic approach. J. Phys. Chem. B 102, 384, 2914-2922.
[30] Ouhenia, S., Chateigner, D., Belkhir, M.A., Guilmeau, E. and Krauss, C.
(2008) Synthesis of calcium carbonate polymorphs in the presence of
polyacrylic acid. J. Cryst. Growth 310, 2832 -2841.
[31] Garcia-Carmona, J., Morales, J.G., and Clemente, R.R. (2003)
Morphological control of precipitated calcite obtained by adjusting the
electrical conductivity in the Ca(OH)2-H2O-CO2 system . J. Cryst.
Growth 249, 561-571.
[32] Vinoba, M., Kim, D. H., Lim, K. S., Jeong, S. K., Lee, S. W. and Alagar,
M. (2011) Biomimetic sequestration of CO2 and reformation to CACO3
using bovine carbonic anhydrase immobilized on SBA-15. Energy Fuels
25, 438-445.
@article{"International Journal of Biological, Life and Agricultural Sciences:57174", author = "Seung Pil Pack", title = "Codon-optimized Carbonic Anhydrase from Dunaliella species: Expression and Characterization", abstract = "Carbonic anhydrases (CAs) has been focused as
biological catalysis for CO2 sequestration process because it can
catalyze the conversion of CO2 to bicarbonate. Here, codon-optimized
sequence of α type-CA cloned from Duneliala species. (DsCAopt) was
constructed, expressed, and characterized. The expression level in E.
coli BL21(DE3) was better for codon-optimized DsCAopt than intact
sequence of DsCAopt. DsCAopt enzyme shows high-stability at pH
7.6/10.0. In final, we demonstrated that in the Ca2+ solution, DsCAopt
enzyme can catalyze well the conversion of CO2 to CaCO3, as the
calcite form.", keywords = "Carbonic anhydrase, Codon-optimization,
Duneliala species, CO2 sequestration", volume = "6", number = "8", pages = "598-5", }
