Blood Elements Activation in Hemodialysis – Animal Model Studies
Haemodialysis (HD) is a procedure saving patient lives around the world, unfortunately it brings numerous complications. Oxidative stress is one of the major factors which lead to erythrocytes destruction during extracorporeal circulation. Repeated HD procedures destroy blood elements and the organism is not able to keep up with their production. 30 HD procedures on healthy sheep were performed to evaluate effects of such treatment. Oxidative stress study was performed together with an analysis of basic blood parameters and empirical assessment of dialyzer condition after the procedure. A reversible decline in absolute leukocyte count, during first 30 min of HD, was observed. Blood clots were formed in the area of the blood inlet and outlet of the dialyzer. Our results are consistent with outcomes presented throughout the literature specifically with respect to the effects observed in humans and will provide a basis to evaluate methods for blood protection during haemodialysis.
[1] M. Nałęcz, Ed., Biocybernetyka i inŜynieria biomedyczna 2000 Tom 3
Sztuczne narządy, Warszawa: Akademicka Oficyna Wydawnicza EXIT,
2001, pp. 218-226,
[2] R. Tadeusiewicz Ed., P. Augustyniak Ed., Podstawy inŜynierii
biomedycznej Tom II, Kraków: Wydawnictwa AGH, 2009, pp. 135-143,
[3] W. A. Nockher, J. Wiemer, J. E. Scherberich, Haemodialysis
monocytopenia: differential sequestration kinetics of CD141CD161 and
CD1411 blood monocyte subsets, Clin. Exp. Immunol., 123 (2001), pp.
49-55,
[4] K. Kose, P. Dogan, Z. Gunduz, R.Dusunsel, C. Z. Utas, Oxidative Stress
in Hemodialyzed Patients and the Long-Term Effects of Dialyzer Reuse
Practice, Clinical Biochemistry, Vol. 30, No. 8, 1997, pp. 601–606,
[5] S. Túri, I. Nemeth, I. Vargha, B. Matkovies, E. Dobos, Erythrocyte
defense mechanisms against free oxygen radicals in hemodialyzed
uremic children, Pediatr. Nephrol., 5 (1991), pp. 179–183,
[6] L. Chludzińska, E. Ananicz, A. Jarosławska, M. Komorowska, Near
infrared radiation protects the red cell membrane against oxidation,
Blood Cells, Molecules and Diseases, 35 (2005), pp. 74-79,
[7] R. L. Heath, L. Packer, Photoperoxidation in isolated chloroplasts.
Kinetic and stoichiometry of fatty acid peroxidation, Arch. Biochem.
Biophys. 125 (1968), pp. 189– 198,
[8] R. Hällgren, P. Venge, B. G. Danielson Neutrophil and eosinophil
degranulation during hemodialysis are mediated by the dialysis
membrane, Nephron, 32:1982, pp. 329–334,
[9] L. S. Kaplow, J. A. Goffinet, Profound neutropenia during the early
phase of hemodialysis. J. Am. Med. Assoc. 203 (1968), pp. 1135-1137,
[10] P. R. Craddock, J. Ferh, A. P. Dalmasso et al., Hemodialysis
leukopenia: pulmonary vascular leukostasis resulting from complement
activation by dialyzer cellophane membranes. J. Clin. Invest., 59 (1977),
pp. 879-888,
[11] A. T. Nguyen, C. Lethias, J. Zingraff, A. Herbelin, C. Naret, B.
Descamps-Latscha, Hemodialysis membrane-induced activation of
phagocyte oxidative metabolism detected in vivo and in vitro within
microamounts of whole blood., Kidney Int., 28 (1985), pp. 158–167,
[12] E. Stoyanova, S. B. Sandoval, L. A. Zuniga, N. El-Yamani, E. Coll, S.
Pastor, J. Reyes, E. Andre´s, J. Ballarin, N. Xamena, R. Marcos,
Oxidative DNA damage in chronic renal failure patients. Nephrol. Dial.
Transplant. 25 (2010), pp. 879 – 885,
[13] R. L. Levine, Carbonyl modified proteins in cellular regulations, aging,
and disease. Free Radic. Biol. Med. 32(2002), pp. 790–796.
[1] M. Nałęcz, Ed., Biocybernetyka i inŜynieria biomedyczna 2000 Tom 3
Sztuczne narządy, Warszawa: Akademicka Oficyna Wydawnicza EXIT,
2001, pp. 218-226,
[2] R. Tadeusiewicz Ed., P. Augustyniak Ed., Podstawy inŜynierii
biomedycznej Tom II, Kraków: Wydawnictwa AGH, 2009, pp. 135-143,
[3] W. A. Nockher, J. Wiemer, J. E. Scherberich, Haemodialysis
monocytopenia: differential sequestration kinetics of CD141CD161 and
CD1411 blood monocyte subsets, Clin. Exp. Immunol., 123 (2001), pp.
49-55,
[4] K. Kose, P. Dogan, Z. Gunduz, R.Dusunsel, C. Z. Utas, Oxidative Stress
in Hemodialyzed Patients and the Long-Term Effects of Dialyzer Reuse
Practice, Clinical Biochemistry, Vol. 30, No. 8, 1997, pp. 601–606,
[5] S. Túri, I. Nemeth, I. Vargha, B. Matkovies, E. Dobos, Erythrocyte
defense mechanisms against free oxygen radicals in hemodialyzed
uremic children, Pediatr. Nephrol., 5 (1991), pp. 179–183,
[6] L. Chludzińska, E. Ananicz, A. Jarosławska, M. Komorowska, Near
infrared radiation protects the red cell membrane against oxidation,
Blood Cells, Molecules and Diseases, 35 (2005), pp. 74-79,
[7] R. L. Heath, L. Packer, Photoperoxidation in isolated chloroplasts.
Kinetic and stoichiometry of fatty acid peroxidation, Arch. Biochem.
Biophys. 125 (1968), pp. 189– 198,
[8] R. Hällgren, P. Venge, B. G. Danielson Neutrophil and eosinophil
degranulation during hemodialysis are mediated by the dialysis
membrane, Nephron, 32:1982, pp. 329–334,
[9] L. S. Kaplow, J. A. Goffinet, Profound neutropenia during the early
phase of hemodialysis. J. Am. Med. Assoc. 203 (1968), pp. 1135-1137,
[10] P. R. Craddock, J. Ferh, A. P. Dalmasso et al., Hemodialysis
leukopenia: pulmonary vascular leukostasis resulting from complement
activation by dialyzer cellophane membranes. J. Clin. Invest., 59 (1977),
pp. 879-888,
[11] A. T. Nguyen, C. Lethias, J. Zingraff, A. Herbelin, C. Naret, B.
Descamps-Latscha, Hemodialysis membrane-induced activation of
phagocyte oxidative metabolism detected in vivo and in vitro within
microamounts of whole blood., Kidney Int., 28 (1985), pp. 158–167,
[12] E. Stoyanova, S. B. Sandoval, L. A. Zuniga, N. El-Yamani, E. Coll, S.
Pastor, J. Reyes, E. Andre´s, J. Ballarin, N. Xamena, R. Marcos,
Oxidative DNA damage in chronic renal failure patients. Nephrol. Dial.
Transplant. 25 (2010), pp. 879 – 885,
[13] R. L. Levine, Carbonyl modified proteins in cellular regulations, aging,
and disease. Free Radic. Biol. Med. 32(2002), pp. 790–796.
@article{"International Journal of Medical, Medicine and Health Sciences:65190", author = "Karolina Grzeszczuk-Kuć and Jolanta Bujok and Tomasz Walski and Małgorzata Komorowska", title = "Blood Elements Activation in Hemodialysis – Animal Model Studies", abstract = "Haemodialysis (HD) is a procedure saving patient lives around the world, unfortunately it brings numerous complications. Oxidative stress is one of the major factors which lead to erythrocytes destruction during extracorporeal circulation. Repeated HD procedures destroy blood elements and the organism is not able to keep up with their production. 30 HD procedures on healthy sheep were performed to evaluate effects of such treatment. Oxidative stress study was performed together with an analysis of basic blood parameters and empirical assessment of dialyzer condition after the procedure. A reversible decline in absolute leukocyte count, during first 30 min of HD, was observed. Blood clots were formed in the area of the blood inlet and outlet of the dialyzer. Our results are consistent with outcomes presented throughout the literature specifically with respect to the effects observed in humans and will provide a basis to evaluate methods for blood protection during haemodialysis.
", keywords = "Animal model, blood components, haemodialysis, leukocytes, oxidative stress, sheep.", volume = "7", number = "9", pages = "594-5", }
