Aureobasidium pullulans Used as a Biological Control Agent under Field Conditions Affects the Microbial Quality of Winter Wheat Grain
The biological activity of A. pullulans isolates against
species of the genus Fusarium, bacteria of the genus Azotobacter and
pseudomonads colonizing wheat kernels was evaluated. A field
experiment was carried out in 2009-2011, in north-eastern Poland.
Winter wheat (cv. Bogatka) plants were sprayed with a cell
suspension of A. pullulans at a density of 106 - 108 per cm3 water at
the stem elongation stage and the heading stage. Untreated plants
served as control. The abundance of epiphytic yeasts, bacteria of the
genus Azotobacter, pseudomonads and Fusarium pathogens on wheat
grain was estimated at harvest and after six months’ storage. The
average size of yeast communities was significantly greater on wheat
kernels treated with a cell suspension of A. pullulans, compared with
control samples. In 2010-2011, biological control reduced the
abundance of some species of the genus Fusarium.
<p>[1] L.K. Berghofer, A.D. Hocking, D. Miskelly, E. Jansson, “Microbiology of
wheat and flour milling in Australia” International Journal of Food
Microbiology 85, 2003, pp. 157-149.
[2] J.R. De Freitas, “Yield and N assimilation of winter wheat (Triticum
aestivum L., var. Norstar) inoculated with rhizobacteria” Pedobiologia
44, 2000, pp. 97-104..
[3] U.A. Druvefors, J. Schnürer, “Mold-inhibitory activity of different yeast
species during airtight storage of wheat grain” FEMS Yeast Research 5,
2004, pp. 373-378.
[4] M.B. Ellis, “Demataceous hyphomycetes” The Eastern Press, London
1971, pp. 608.
[5] G.H. Fleet, “Yeasts in foods and beverages: impact on product quality
and safety” Curr. Opin. Biotechnol. 18 (2), 2007, pp. 170-175.
[6] M. Gniewosz, W. Duszkiewicz-Reinhard, “Comparative studies on
pullulan synthesis, melanin synthesis and morphology of white mutant
Aureobasidium pullulans B-1 and parent strain A.p.-3” Carbohydrate
Polymers72, 2008, pp. 431–438.
[7] E. King, M.K. Ward, D.E. Raney, “Two simple media for the
demonstration of pyocyanin and fluorescein” J. Lab. Clin. Med. 44,
1954, pp. 301–307.
[8] V. Kumar, R.K. Behl, N. Narula, “Establishment of phosphatesolubilizing
strains of Azotobacter chroococcum in the rhizosphere and
their effect on wheat cultivars under green house conditions” Microbiol
Res. 156, 2001, pp. 87-93.
[9] J.F. Leslie, B.A. Summerrell, “The Fusarium laboratory manual”
Blackwell Publishing, Oxford, 2006, pp. 388.
[10] J.P. Martin, “Use of acid, rose Bengal and streptomycyn in the plate
metod for estimating soil fungi” Soil Sci. 38, 1950, pp. 215-220.
[11] S. Martyniuk, M. Martyniuk, “Occurence of Azotobacter spp” in Polish
soils. Polish J. Environ. Stud. 12 (3), 2003, pp. 371–374.
[12] M. Olstrope, J. Borling, J. Schnurer, V. Passoth, “ Pichia anomala yeast
improves feed hygiene during storage of moist crimped barley grain
under Swedish farm conditions” Animal Feed Science and Technology
156, 2010, pp. 37–46.
[13] L. Schena, F. Nigro, I. Pentimone, A. Ligorio, A. Ippolito, “Control of
postharvest rots of sweet cherries and table grapes with endophytic
isolates of Aureobasidium pullulans” Postharvest Biology and
Technology, 30, 2003, pp. 209–220.
[14] D.A. Schisler, P.J. Slininger, M.J. Boehm, P.A. Paul, “Co-culture of
Yeast Antagonists of Fusarium Head Blight and their Effect on Disease
Development in Wheat” Plant Pathology Journal 10 (4), 2011, pp. 128–
137.
[15] S. Vero , P. Mondino, J. Burguen˜o, M. Soubes, M. Wisniewski,
“Characterization of biocontrol activity of two yeast strains from
Uruguay against blue mold of apple” Postharvest Biology and
Technology 26(1), 2002, pp. 91–98.
[16] U. Wachowska, K. Kucharska, M. Jędryczka, N. Łobik,
“Microorganisms as biological control agents against Fusarium
pathogens in winter wheat” Pol. J. Environ. Stud. 22 (2), 2013, pp. 591–
597.
[17] S. Zhang, D.A. Schisler, M.J. Boehm, P.J. Slininger, “Utilization of
chemical inducers of resistance and Cryptococcus flavescens OH 182.9
to reduce Fusarium head blight under greenhouse conditions” Biological
Control 42, 2007, pp. 308-315.</p>
<p>[1] L.K. Berghofer, A.D. Hocking, D. Miskelly, E. Jansson, “Microbiology of
wheat and flour milling in Australia” International Journal of Food
Microbiology 85, 2003, pp. 157-149.
[2] J.R. De Freitas, “Yield and N assimilation of winter wheat (Triticum
aestivum L., var. Norstar) inoculated with rhizobacteria” Pedobiologia
44, 2000, pp. 97-104..
[3] U.A. Druvefors, J. Schnürer, “Mold-inhibitory activity of different yeast
species during airtight storage of wheat grain” FEMS Yeast Research 5,
2004, pp. 373-378.
[4] M.B. Ellis, “Demataceous hyphomycetes” The Eastern Press, London
1971, pp. 608.
[5] G.H. Fleet, “Yeasts in foods and beverages: impact on product quality
and safety” Curr. Opin. Biotechnol. 18 (2), 2007, pp. 170-175.
[6] M. Gniewosz, W. Duszkiewicz-Reinhard, “Comparative studies on
pullulan synthesis, melanin synthesis and morphology of white mutant
Aureobasidium pullulans B-1 and parent strain A.p.-3” Carbohydrate
Polymers72, 2008, pp. 431–438.
[7] E. King, M.K. Ward, D.E. Raney, “Two simple media for the
demonstration of pyocyanin and fluorescein” J. Lab. Clin. Med. 44,
1954, pp. 301–307.
[8] V. Kumar, R.K. Behl, N. Narula, “Establishment of phosphatesolubilizing
strains of Azotobacter chroococcum in the rhizosphere and
their effect on wheat cultivars under green house conditions” Microbiol
Res. 156, 2001, pp. 87-93.
[9] J.F. Leslie, B.A. Summerrell, “The Fusarium laboratory manual”
Blackwell Publishing, Oxford, 2006, pp. 388.
[10] J.P. Martin, “Use of acid, rose Bengal and streptomycyn in the plate
metod for estimating soil fungi” Soil Sci. 38, 1950, pp. 215-220.
[11] S. Martyniuk, M. Martyniuk, “Occurence of Azotobacter spp” in Polish
soils. Polish J. Environ. Stud. 12 (3), 2003, pp. 371–374.
[12] M. Olstrope, J. Borling, J. Schnurer, V. Passoth, “ Pichia anomala yeast
improves feed hygiene during storage of moist crimped barley grain
under Swedish farm conditions” Animal Feed Science and Technology
156, 2010, pp. 37–46.
[13] L. Schena, F. Nigro, I. Pentimone, A. Ligorio, A. Ippolito, “Control of
postharvest rots of sweet cherries and table grapes with endophytic
isolates of Aureobasidium pullulans” Postharvest Biology and
Technology, 30, 2003, pp. 209–220.
[14] D.A. Schisler, P.J. Slininger, M.J. Boehm, P.A. Paul, “Co-culture of
Yeast Antagonists of Fusarium Head Blight and their Effect on Disease
Development in Wheat” Plant Pathology Journal 10 (4), 2011, pp. 128–
137.
[15] S. Vero , P. Mondino, J. Burguen˜o, M. Soubes, M. Wisniewski,
“Characterization of biocontrol activity of two yeast strains from
Uruguay against blue mold of apple” Postharvest Biology and
Technology 26(1), 2002, pp. 91–98.
[16] U. Wachowska, K. Kucharska, M. Jędryczka, N. Łobik,
“Microorganisms as biological control agents against Fusarium
pathogens in winter wheat” Pol. J. Environ. Stud. 22 (2), 2013, pp. 591–
597.
[17] S. Zhang, D.A. Schisler, M.J. Boehm, P.J. Slininger, “Utilization of
chemical inducers of resistance and Cryptococcus flavescens OH 182.9
to reduce Fusarium head blight under greenhouse conditions” Biological
Control 42, 2007, pp. 308-315.</p>
@article{"International Journal of Biological, Life and Agricultural Sciences:53460", author = "Urszula Wachowska and Anna Daria Stasiulewicz-Paluch and Iwona Konopka and Katarzyna Kucharska and Justyna Borowska", title = "Aureobasidium pullulans Used as a Biological Control Agent under Field Conditions Affects the Microbial Quality of Winter Wheat Grain", abstract = "The biological activity of A. pullulans isolates against
species of the genus Fusarium, bacteria of the genus Azotobacter and
pseudomonads colonizing wheat kernels was evaluated. A field
experiment was carried out in 2009-2011, in north-eastern Poland.
Winter wheat (cv. Bogatka) plants were sprayed with a cell
suspension of A. pullulans at a density of 106 - 108 per cm3 water at
the stem elongation stage and the heading stage. Untreated plants
served as control. The abundance of epiphytic yeasts, bacteria of the
genus Azotobacter, pseudomonads and Fusarium pathogens on wheat
grain was estimated at harvest and after six months’ storage. The
average size of yeast communities was significantly greater on wheat
kernels treated with a cell suspension of A. pullulans, compared with
control samples. In 2010-2011, biological control reduced the
abundance of some species of the genus Fusarium.
", keywords = "Aureobasidium pullulans, winter wheat grain,
Fusarium, bacteria.", volume = "7", number = "7", pages = "520-4", }
