Antimicrobial Potentials of Flavonoids Isolated from Tagetes erecta
In this study, we are interested in a species of the
family of Asteraceae (Tagetes erecta). This family is considered as a
source of antimicrobial extracts with strong capacity. The extraction
of the flavonoids is carried out by the method of liquid/liquid with the
use of successive solvents. Afterwards, we evaluated the biological
activity of the flavonoids on five pathogenic bacterial stocks such as
Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae,
Pseudomonas aeruginosa and Staphylococcus aureus and two stocks
of yeasts to knowing Candida albicans) and Saccharomyces
cerevisiae, by employing the method of the aromatogramme starting
from a solid disc. The result of the antimicrobial activity shows an
action and a variable degree of sensitivity according to bacterial
stocks tested. It will be noted that the flavonoids have an inhibiting
effect on E. coli, B. subtilis, K. pneumoniae and S. aureus. But a
resistance with respect to the extract by P. aeruginosa, C. albicans
and S. cerevisiae is to be mentioned.
[1] C.Muthu, N.Mir,S.Ignacimuthu, “Medicinal plants used by traditional
healers in kanchee puran district of tamil Nado, India”. Journal of
ethnobiology and ethnomedecine. 2006; 2, 43 doi: 10.1186/1746-4269-
2-43.
[2] O. Catier, D. Roux, “Botanique, pharmacogonosie, phytothérapeutie”
.3rd Wolters Kluwer, 2007. p. 13.
[3] D. Lamnaouer, “Composition chimique et activité biologique de
quelques plantes médicinales du PNT”. Programme de l’UICN en
Afrique du nord, 2001. Phase III, p. 1-9.
[4] D. Lamnaouer, “Réflexions sur l’enseignement de la botanique et des
plantes médicinales”. Colloque « Voyages en Botanique » Maroc; 2010.
p.4.
[5] D.S.Teixeira, “Mining the essential oil of the Anthemideae”. African
Journal of Biotechnology.v.3, n.12, p. 706-720, 2004.
[6] L Pacheco, M. M. Costantine, G. D. V. Hankins,“Physiologic changes
during pregnancy,” in Clincal Pharmacology During Pregnancy ed.
Mattison D. R., editor. (San Diego: Academic Press; ) 5–14, 2013.
[7] D.R. Katerere, G. Graziani, K.M. Thembo, N.Z. Nyazema, A.
Ritieni, “Antioxidant activity of some African medicinal and dietary
leafy African vegetables”. Afr. J. Biotechnol.11:4103- 4108, 2012.
[8] I.Farombi, “Africain indigenous plants with chemotherapeutic potentials
and biotechnological approach to the production of bioactive
prophylactic agent”. African Journal of Biotechnology. v. 2, n. 12, p.
662-671, 2003.
[9] W.F.Sayed, C.T.Wheeler,“Effect of the flavonoid quercetin on culture
and isolation of Frankia from Casuarina root nodules”. Folia Microbiol.
v.44, n.1, p. 59-62, 1999.
[10] R.N Sanches, D.A.Garcia Corte, M.S.Schiavini, “An evaluation of
antibacterial activities of Psidium guajava (L.)”.Brazilian Archives of
Biology and Technology. v.48, n.3, p. 429-436, 2005.
[11] A. Hallimi, “Les plantes médicinales en Algérie”. Ed. Berti, Algérie,
2004. p. 42.
[12] N.Behidj-Benyounes, T.Dahmane, K. Hassak, F.Slimani,“The
antibacterial activity of flavonoïds extracted from Crateagus oxyacantha
(L.) leaves of bainem (Algeria)”.13thInternational Multidisciplinary
Scientific GeoConference SGEM Conference proceedings, June 16-22,
2013Albena (Bulgarie). 2013a. p. 288- 295.
[13] N. Behidj-Benyounes, T. Dahmane, K. Hassak, F.Slimani, “The
antibacterial activity of flavonoïds extracted from Crateagus oxyacantha
(L.) flowers of Boumerdès (Algeria)”. 13thInternational
Multidisciplinary Scientific GeoConference SGEM Conference
proceedings. June 16-22, 2013 Albena (Bulgarie). 2013b p. 296- 303.
[14] J. Bruneton, “Plantes toxiques végétaux dangereux pour l’Homme et les
animaux”. 2nd. Tec and Doc, Paris, France, 2001. p. 542 - 564.
[15] J.L. Fauchere, J.L. Avril, “Bactériologie générale et médicale”. ed.
Ellipses, Paris, France, 2002. p. 365.
[16] M.S. Klaric, I.Kosalec, K.J.Mastelic, E.Pieckova, K.Pepeljna,
“Antifungal activity of thyme (Thymus vulgaris L.) essential oil and
thymol against mould from damp dwellings”. Letters in Applied
Microbiology. v.44, p. 36-42, 2006.
[17] M.A.Pfaller, D.Diekema, M.Mendez, C.Kibbler, P.Erzsebet, S.C.Chang,
D.L.Gibbs, V.A.Newell, “The global antifungal surveillance group,
Candida guilliermondii, an opportunistic fungal pathogen with decreased
susceptibility to fluconazole: Geographic and temporal trends from the
ARTEMIS DISK Antifungal Surveillance Program”. Journal Clinical
Microbiology. v.44, p. 3551-3556, 2004.
[18] C.Su, X.Zhahg, J.P.Dudey, “Génotyping of Toxiplasma gontii PCRRFLP
markers: a high resolution simpal method for identification of
parasites”. International Journal of Parasitology. v.36, p. 441-848,
2006.
[19] T.Essawi, M.Srour, “Screening of some Palestinian plants for
antibacterial activity”. Journal of Ethnopharm.v. 70, p. 343-349. 2000.
[20] M.C.Marjorie,“Plants products as antimicrobial agents”. Clinical
Microbiology Reviews. v.9, p. 564-582, 1999.
[21] B.Shan, Y.Z.Cai, J.D.Brooks, H.Corke, “The in vitro antibacterial
activity of dietary spice and medicinal herb extracts”. Journal
International of Food Microbiology. v.117, p. 112-119, 2007.
[22] T. Askun, G. Tumen, F. Satil, M.Ates,“In vitro activity of methanol
extracts of plants used as spices against Mycobacterium tuberculosis and
other bacteria”. Food Chemistry. v. 104, n. 116, p. 289-294, 2009.
[23] D.Horikrrisha, A.V.N.Approo, M.C.Probharkar, “Pharmacological
investigation of flavonoid glucoside”. Indian Journal of
Pharmacology.v.36, p. 244-250, 2004.
[24] S.Rhama, S.Madhavan,“Antibacterial activity of the flavonoid, patulitrin
isolated from the flowers of Tagetes erecta L.”International Journal of
Pharm Tech Research. v.3, n.3, p. 1407-1409, 2010.
[25] M.C. Pibiri, “Assainissement microbiologique de l’air et du système de
ventilation au moyen d’huile essentielle”.Thèse de Doctorat, Ecole
Polytechnique Fédérale de Lausanne, 2006. p. 177.
[26] M.A.Alvarez, N.B.Debattista, NB.Pappano, “Antimicrobial activity and
synergism of some substituted flavonoids”. Folia Microbiol. v. 53, n.1,
p. 23-28, 2008.
[27] S.Rees, J.B.Harborne, “The role of sesquiterpene lactones and phenolics
in the chemical defense of the chicory plant”. Phytochemistry.v.24, p.
2225-2231, 1985.
[1] C.Muthu, N.Mir,S.Ignacimuthu, “Medicinal plants used by traditional
healers in kanchee puran district of tamil Nado, India”. Journal of
ethnobiology and ethnomedecine. 2006; 2, 43 doi: 10.1186/1746-4269-
2-43.
[2] O. Catier, D. Roux, “Botanique, pharmacogonosie, phytothérapeutie”
.3rd Wolters Kluwer, 2007. p. 13.
[3] D. Lamnaouer, “Composition chimique et activité biologique de
quelques plantes médicinales du PNT”. Programme de l’UICN en
Afrique du nord, 2001. Phase III, p. 1-9.
[4] D. Lamnaouer, “Réflexions sur l’enseignement de la botanique et des
plantes médicinales”. Colloque « Voyages en Botanique » Maroc; 2010.
p.4.
[5] D.S.Teixeira, “Mining the essential oil of the Anthemideae”. African
Journal of Biotechnology.v.3, n.12, p. 706-720, 2004.
[6] L Pacheco, M. M. Costantine, G. D. V. Hankins,“Physiologic changes
during pregnancy,” in Clincal Pharmacology During Pregnancy ed.
Mattison D. R., editor. (San Diego: Academic Press; ) 5–14, 2013.
[7] D.R. Katerere, G. Graziani, K.M. Thembo, N.Z. Nyazema, A.
Ritieni, “Antioxidant activity of some African medicinal and dietary
leafy African vegetables”. Afr. J. Biotechnol.11:4103- 4108, 2012.
[8] I.Farombi, “Africain indigenous plants with chemotherapeutic potentials
and biotechnological approach to the production of bioactive
prophylactic agent”. African Journal of Biotechnology. v. 2, n. 12, p.
662-671, 2003.
[9] W.F.Sayed, C.T.Wheeler,“Effect of the flavonoid quercetin on culture
and isolation of Frankia from Casuarina root nodules”. Folia Microbiol.
v.44, n.1, p. 59-62, 1999.
[10] R.N Sanches, D.A.Garcia Corte, M.S.Schiavini, “An evaluation of
antibacterial activities of Psidium guajava (L.)”.Brazilian Archives of
Biology and Technology. v.48, n.3, p. 429-436, 2005.
[11] A. Hallimi, “Les plantes médicinales en Algérie”. Ed. Berti, Algérie,
2004. p. 42.
[12] N.Behidj-Benyounes, T.Dahmane, K. Hassak, F.Slimani,“The
antibacterial activity of flavonoïds extracted from Crateagus oxyacantha
(L.) leaves of bainem (Algeria)”.13thInternational Multidisciplinary
Scientific GeoConference SGEM Conference proceedings, June 16-22,
2013Albena (Bulgarie). 2013a. p. 288- 295.
[13] N. Behidj-Benyounes, T. Dahmane, K. Hassak, F.Slimani, “The
antibacterial activity of flavonoïds extracted from Crateagus oxyacantha
(L.) flowers of Boumerdès (Algeria)”. 13thInternational
Multidisciplinary Scientific GeoConference SGEM Conference
proceedings. June 16-22, 2013 Albena (Bulgarie). 2013b p. 296- 303.
[14] J. Bruneton, “Plantes toxiques végétaux dangereux pour l’Homme et les
animaux”. 2nd. Tec and Doc, Paris, France, 2001. p. 542 - 564.
[15] J.L. Fauchere, J.L. Avril, “Bactériologie générale et médicale”. ed.
Ellipses, Paris, France, 2002. p. 365.
[16] M.S. Klaric, I.Kosalec, K.J.Mastelic, E.Pieckova, K.Pepeljna,
“Antifungal activity of thyme (Thymus vulgaris L.) essential oil and
thymol against mould from damp dwellings”. Letters in Applied
Microbiology. v.44, p. 36-42, 2006.
[17] M.A.Pfaller, D.Diekema, M.Mendez, C.Kibbler, P.Erzsebet, S.C.Chang,
D.L.Gibbs, V.A.Newell, “The global antifungal surveillance group,
Candida guilliermondii, an opportunistic fungal pathogen with decreased
susceptibility to fluconazole: Geographic and temporal trends from the
ARTEMIS DISK Antifungal Surveillance Program”. Journal Clinical
Microbiology. v.44, p. 3551-3556, 2004.
[18] C.Su, X.Zhahg, J.P.Dudey, “Génotyping of Toxiplasma gontii PCRRFLP
markers: a high resolution simpal method for identification of
parasites”. International Journal of Parasitology. v.36, p. 441-848,
2006.
[19] T.Essawi, M.Srour, “Screening of some Palestinian plants for
antibacterial activity”. Journal of Ethnopharm.v. 70, p. 343-349. 2000.
[20] M.C.Marjorie,“Plants products as antimicrobial agents”. Clinical
Microbiology Reviews. v.9, p. 564-582, 1999.
[21] B.Shan, Y.Z.Cai, J.D.Brooks, H.Corke, “The in vitro antibacterial
activity of dietary spice and medicinal herb extracts”. Journal
International of Food Microbiology. v.117, p. 112-119, 2007.
[22] T. Askun, G. Tumen, F. Satil, M.Ates,“In vitro activity of methanol
extracts of plants used as spices against Mycobacterium tuberculosis and
other bacteria”. Food Chemistry. v. 104, n. 116, p. 289-294, 2009.
[23] D.Horikrrisha, A.V.N.Approo, M.C.Probharkar, “Pharmacological
investigation of flavonoid glucoside”. Indian Journal of
Pharmacology.v.36, p. 244-250, 2004.
[24] S.Rhama, S.Madhavan,“Antibacterial activity of the flavonoid, patulitrin
isolated from the flowers of Tagetes erecta L.”International Journal of
Pharm Tech Research. v.3, n.3, p. 1407-1409, 2010.
[25] M.C. Pibiri, “Assainissement microbiologique de l’air et du système de
ventilation au moyen d’huile essentielle”.Thèse de Doctorat, Ecole
Polytechnique Fédérale de Lausanne, 2006. p. 177.
[26] M.A.Alvarez, N.B.Debattista, NB.Pappano, “Antimicrobial activity and
synergism of some substituted flavonoids”. Folia Microbiol. v. 53, n.1,
p. 23-28, 2008.
[27] S.Rees, J.B.Harborne, “The role of sesquiterpene lactones and phenolics
in the chemical defense of the chicory plant”. Phytochemistry.v.24, p.
2225-2231, 1985.
@article{"International Journal of Biological, Life and Agricultural Sciences:68790", author = "N. Behidj-Benyounes and S. Bennaamane and F. Zohra Bissaad and N. Chebouti and H. Mohandkaci and N. Abdalaziz and S. Iddou", title = "Antimicrobial Potentials of Flavonoids Isolated from Tagetes erecta", abstract = "In this study, we are interested in a species of the
family of Asteraceae (Tagetes erecta). This family is considered as a
source of antimicrobial extracts with strong capacity. The extraction
of the flavonoids is carried out by the method of liquid/liquid with the
use of successive solvents. Afterwards, we evaluated the biological
activity of the flavonoids on five pathogenic bacterial stocks such as
Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae,
Pseudomonas aeruginosa and Staphylococcus aureus and two stocks
of yeasts to knowing Candida albicans) and Saccharomyces
cerevisiae, by employing the method of the aromatogramme starting
from a solid disc. The result of the antimicrobial activity shows an
action and a variable degree of sensitivity according to bacterial
stocks tested. It will be noted that the flavonoids have an inhibiting
effect on E. coli, B. subtilis, K. pneumoniae and S. aureus. But a
resistance with respect to the extract by P. aeruginosa, C. albicans
and S. cerevisiae is to be mentioned.
", keywords = "Antimicrobial activity, flavonoids, microbial strains, Tagetes erecta L.", volume = "8", number = "11", pages = "1265-4", }
